MRI软骨下骨髓水肿与早期膝骨关节炎临床表现的相关性研究?

目的：探讨早期膝骨关节炎(KOA)MRI 软骨下骨髓水肿的程度,并探究骨髓水肿与 KOA 夜间休息痛、关节肿胀程度和骨关节炎功能(the western Ontario and McMaster Universities osteoarthritis index,WOMAC)评分的相关性。方法本研究按纳排标准收集了早期 KOA 病例60例,其中伴骨髓水肿改变30例,设为观察组,不伴骨髓水肿改变30例,设为对照组。观察骨髓水肿程度评分、夜间休息痛视觉模拟(visular analogue scale, VAS)评分、关节肿胀程度及 WOMAC 功能评分情况。结果1)两组患者在夜间休息痛 VAS 评分、关节肿胀程度和 WOMAC 评分上比较,差异有统计学意义(P <0.05);2)观察组骨髓水肿程度越重,夜间休息痛 VAS 评分与WOMAC 评分越高;3)观察组骨髓水肿和夜间休息痛情况的相关系数 r =0.933(r >0)(P <0.05),二者呈正相关性;骨髓水肿与 WOMAC 评分的相关系数 r=0.555(r>0)(P <0.05),二者呈正相关性;骨髓水肿同关节肿胀程度相关系数 r=0.148(r>0)(P >0.05),二者间无明显相关性。结论MRI 软骨下骨髓水肿样改变与早期 KOA 夜间休息痛、WOMAC 评分具有正相关性;与关节肿胀情况无相关性。     

2006～2007年桐乡市不同人群HIV监测检测结果分析

目的:分析不同人群HIV监测结果及感染者特征,制定有效的防控措施。方法:采用EXECL数据库对2006~2007年桐乡市各艾滋病实验室检测数据和网络直报个案资料进行整理、分析。结果:2006年检测38674份血清,确认HIV抗体阳性为10例,占0.03%,2007年检测37287份,确认HIV抗体阳性为6例,占0.02%,HIV阳性者的家属的检测确认阳性率都最高,分别为14.29%和16.67%。感染者特征中21~25岁为感染主要年龄段,以无业为主,以性行为为HIV传播的高危行为,住在城镇较多。结论:桐乡市的艾滋病处于低流行水平,且以特定人群为主,有向一般人群扩散的趋势,故要继续加强监测并采取以开展健康教育为主的干预,特别是面向农村和农民,提高自我防范意识,以遏制艾滋病在我市的流行和蔓延。     

苏州地区城乡生活饮用水水质调查与分析

目的掌握苏州地区城镇与农村生活饮用水水质卫生状况及水质指标自然本底值,为建立健全城乡生活饮用水质监测网络提供依据。方法按现行《生活饮用水标准检验方法》（GB／T5750．2）中《水样的采集与保存》,于2010年丰水期在沿长江和环太湖等典型地区采集城镇和农村集中式供水单位的出厂水水样,按《生活饮用水标准检验方法》（GB／T5750—2006）进行水质全项目检测分析。结果2010年丰水期苏州地区城乡饮用水主要不合格指标为铝和三氯乙醛,主要理化常规指标铅、氟化物、硝酸盐、三氯甲烷、铝、氯化物、硫酸盐、溶解性总固体、总硬度、耗氧量等本底值,分别为0．0014、0．62、1．21、0．0046、0．17、48．6、70．0、288．3、118．1、1．44mg／L。结论苏州沿长江和环太湖等典型地区城乡饮用水水质指标基本符合《生活饮用水卫生标准》（GB5749—2006）水质要求,能够保障城乡居民饮水安全及社会发展需求。     

骨髓增生异常综合征难治性贫血与巨幼细胞贫血细胞形态学比较

目的通过对骨髓增生异常综合征--难治性贫血(MDS-RA)和巨幼细胞贫血(MA)进行细胞形态学比较,提高其诊断与鉴别水平。方法对23例MDS-RA与28例MA患者进行常规骨髓穿刺涂片,进行瑞氏染色光学显微镜下观察比较。结果MDS-RA以双核红细胞及奇数核红细胞、淋巴样小巨核最有特征。MA骨髓红系呈明显的“幼核老浆”改变;粒系以中幼粒以下各阶段细胞巨型改变;未发现淋巴杆小巨核细胞。结论淋巴样小巨核细胞是MDS-RA主要特征;MA骨髓细胞呈”幼核老桨”,并以红系为明显。     

Th17 cell‐derived miR‐155‐5p modulates interleukin‐17 and suppressor of cytokines signaling 1 expression during the progression of systemic sclerosis

Background miR‐155‐5p is associated with autoimmune diseases. T helper 17 (Th17) cells, interleukin (IL)‐17, and suppressor of cytokines signaling 1 (SOCS1) have important roles in the pathogenesis of systemic sclerosis (SSc). The purpose of this study was to explore the role of miR‐155‐5p in the regulation of IL‐17 and SOCS1 expression in Th17 cells and the subsequent effect on SSc disease progression.MethodsTh17 cells were isolated from peripheral blood mononuclear cells of SSc patients and healthy controls (HCs). RT‐qPCR and western blotting were used to examine the expression patterns of miR‐155‐5p, IL‐17, and SOCS1. Luciferase reporter assays were performed to confirm SOCS1 as a target of miR‐155‐5p. RNA pull‐down assays were performed to detect the interaction of IL‐17 and SOCS1 with miR‐155‐5p. In situ hybridization was performed to analyze the co‐expression pattern of miR‐155‐5p and IL17A in Th17 cells.ResultsThe levels of Th17 cell‐derived miR‐155‐5p were significantly up‐regulated in SSc patients compared with HCs, and its levels were negatively correlated with SOCS1 levels. Meanwhile, miR‐155‐5p positively regulated IL‐17 expression levels in Th17 cells isolated from SSc patients as the disease progressed. Using pmirGLO vectors, SOCS1 was confirmed as a target of miR‐155‐5p. The binding status of IL‐17 and SOCS1 to miR‐155‐5p was related to SSc progression. An increase in the co‐localization of miR‐155‐5p and IL‐17 was associated with greater SSc progression.ConclusionsIL‐17 and SOCS1 expression modulated by Th17 cell‐derived miR‐155‐5p are critical for SSc progression, which may provide novel insights into the pathogenesis of SSc.     

170例肝移植病例医疗质量费用分析

目的:分析肝移植病例住院费用构成并评价其医疗质量.方法:收集手术操作代码为50.5001的病案170份,采用<病例分型质量费用管理系统>软件和SAS6.1 2,进行描述性统计分析.结果与讨论:肝移植治疗水平不断提高,但费用十分昂贵,必须采取各种有效措施,降低患者负担.     

台州市2018/2019年度社区老年人接种流感疫苗的保护效果

目的评估台州市2018/2019年度社区老年人接种三价灭活流感疫苗(TIV)的保护效果(VE)。方法运用前瞻性队列研究设计,招募接种和未接种TIV的≥60岁社区老年人随访6个月,观察流感样疾病(ILI)、因ILI就诊、因ILI或肺炎住院、因呼吸或循环系统疾病住院4种临床结局,计算TIV的VE。结果 TIV接种组、未接种组分别纳入研究对象1 048名、1 025名。接种TIV后1-3月预防4种临床结局的VE(95%CI)分别为-25.1(-80.4-13.2)%、-33.1(-99.2-11.1)%、35.8(-124.9-81.7)%和-12.6(-229.2-61.5)%;接种TIV后4-6月分别为25.5(-7.5-48.4)%、35.1(3.0-56.5)%、1.4(-249.1-72.1)%和-11.5(-240.9-63.5)%;接种TIV后1-6月分别为5.9(-24.2-28.7)%、11.6(-18.9-34.3)%、21.7(-99.2-69.2)%和-13.9(-155.5-49.2)%。结论台州市2018/2019年度社区老年人接种TIV对预防ILI病例发病、就诊和相关住院具有一定的保护效果。     

骨折相关感染治疗的研究进展

骨折相关感染(FRI)是骨科常见的手术并发症,FRI发生的多样性以及近年来对抗生素的滥用给FRI的诊断和治疗带来了一定难度.术前体格检查、影像学检查及相关的实验室检查对明确诊断具有重要意义.治疗方法主要以静脉应用抗生素、局部清创、术中植入抗生素载体为主,目前临床应用效果最好且满意度较高的是术中植入抗生素载体,其具有较好...     

Early postoperative pain after subxiphoid uniportal thoracoscopic major lung resection: a prospective,single- blinded,randomized controlled trial

Open in a separate window OBJECTIVESPreoperative selection bias led to the inability to generalize the proposed benefit of subxiphoid uniportal video-assisted thoracoscopic surgery (SVATS) as having less postoperative pain than uniportal intercostal VATS. So, we conducted this prospective, single-blinded, randomized controlled trial to investigate the hypothesis that SVATS may have less early postoperative pain than UVATS in patients who undergo major lung resection for early-stage lung cancer.METHODSA total of262 patients were randomly allocated between 2 groups (each with 131 patients), the first being the UVATS group and the second being the SVATS group. The values indicated on the numerical rating scale (NRS) of pain were collected at 24 h and 48 h during rest and during coughing. In addition, different perioperative variables were analysed and compared between the 2 groups.RESULTSMultiple linear regression analysis showed that the type of surgical approach was a significant predictor of the postoperative NRS values. The postoperative NRS pain values were significantly lower in the SVATS group after 24 h during rest and coughing and after 48 h during coughing. Postoperatively, patients in the SVATS group got out of bed significantly earlier [16.37 (2.54) vs 18.05 (3.29) h, p < 0.001]. The SVATS group showed a significantly higher rate of intraoperative arrhythmia [20 (15.3%) vs 3 (2.3%) patients, p = 0.03].CONCLUSIONSSVATS major pulmonary resection in early-stage lung cancer is associated with less early postoperative pain than the UVATS approach. Operating on patients with cardiac problems using the SVATS approach is still a limiting factor for randomization due to the potential compression on the heart with resulting arrhythmia.Clinical trial registrationThe trial was registered under clinical trials.gov Identifier: {"type":"clinical-trial","attrs":{"text":"NCT03331588","term_id":"NCT03331588"}}NCT03331588. https://clinicaltrials.gov/ct2/show/{"type":"clinical-trial","attrs":{"text":"NCT03331588","term_id":"NCT03331588"}}NCT03331588.     

Targeted peptide-modified oxidized mesoporous carbon nanospheres for chemo-thermo combined therapy of ovarian cancer in vitro

Ovarian cancer remains one of serious hazards to human health due to many drawbacks of existing available treatment options. In this study, a multifunctional chemo-thermo combined therapy nanoplatform (OMCNPID) was successfully prepared, which is composed of I₆P₈ peptide as a targeting moiety to interleukin-6 receptors (IL-6Rs), oxidized mesoporous carbon nanospheres (OMCN) as a near infrared (NIR)-triggered drug carrier and doxorubicin (DOX) as a chemotherapeutic drug and fluorescent agent. The synthesized multifunctional nanoplatform displayed high storage capacity for drugs and excellent photothermal properties. Besides, DOX was rapidly released from OMCNPID at the condition of low pH and NIR laser irradiation due to the dissociation of DOX from graphitic cores of OMCN. In vitro experimental results verified that OMCNPID could be markedly taken up by SKOV-3 monolayer cells and tumor spheroids, and revealed a remarkable synergistic chemo-photothermal effect against ovarian cancer. All the results demonstrated that OMCNPID is a pH/NIR dual-stimulus responsive nanoplatform and can achieve efficient chemo-thermo combined therapy.