Simultaneous determination of benzocaine and cetylpiridinium chloride in tablets by first-derivative spectrophotometric method

A new spectrophotometric method for the simultaneous determination of benzocaine and cetylpiridinium chloride in pharmaceutical tablets, which does not require any preliminary separation or treatment of the samples, is described. The quantitative determination of both drugs was carried out using the first derivative values measured at 231.40 and 310.00 nm for benzocaine and at 220.70 nm for cetylpiridinium chloride using the zero-crossing method. The calibration graphs were linear in the ranges from 10 to 25 mg/l of benzocaine and from 4 to 20 mg/l of cetylpiridinium chloride. The developed method was successfully applied for the assay of pharmaceutical tablets and proved to be simple, sensitive and selective. Thermogravimetric techniques, Karl Fischer and loss on drying were also used for a stoichiometric evaluation of the substances studied.     

Inhibitory effect of buspirone and diazepam, but not of 8-OH-DPAT, on maternal behavior and aggression

The action of diazepam (0.0, 1.0, and 2.0 mg/kg) and the serotonergic compounds buspirone (0.0, 2.5, and 5.0 mg/kg) and 8-OH-DPAT (0.0, 0.1, and 1.0 mg/kg) on maternal behavior and aggression were studied. An activity test was made after these treatments to control for unspecific actions due to motor impairment. Diazepam and buspirone dose-dependently inhibited the expression of maternal aggression and the active components of maternal behavior such as retrieving and nest building. 8-OH-DPAT did not affect these behaviors. 8-OH-DPAT (1.0 mg/kg) provoked the serotonergic syndrome and hypothermia; however, ovariectomized animals showed more signs of the syndrome and a decrease in body temperature after 8-OH-DPAT than lactating rats. Buspirone, but not the other anxiolytics, reduced motor activity. The role of drugs acting at the serotonergic, dopaminergic, and GABA-benzodiazepine systems in the control of maternal behavior and aggression is discussed.     

Coeliac disease in identical twins.

Coeliac disease occurred at the same age in MZ twins. The diagnosis was confirmed by histology of the small intestine, rapid response to a gluten-free diet, and relapse after reintroduction of gluten.     

Pharmacological activity and chemical composition of callus culture extracts from selected species of Phyllanthus

This study was conducted in order to determine the chemical composition and the possible antinociceptive effects in mice of some species of Phyllanthus in vitro. The methanolic extracts obtained from callus cultures of P. fraternus, P. stipulatus and P. caroliniensis caused significant inhibition in to the late phase of the formalin test, whereas the extract from P. urinaria inhibited both neurogenic and inflammatory phases of the test. Conventional chromatographic methods (TLC, GC) permitted the detection of some steroids or triterpenes, including beta-sitosterol, glochidonol and glochidone, which seem be responsible for the antinociceptive effects of the callus extracts studied.     

Oral and Genital Human Herpesvirus 8 and Human Papillomavirus in heterosexual partners

Background: The purpose of this study was to verify a possible co‐infection of human herpesvirus 8 (HHV‐8) in commonly associated human papillomavirus (HPV) penile lesions and to determine the frequency of detection of these viruses in the oral mucosa of their female counterparts. Methods: Thirty‐one male subjects underwent penile swabs from clinical HPV‐related lesions. Their female counterparts underwent swabs of the vagina, uterine cervix, and oral mucosa. HPV and HHV‐8 detection was performed by polymerase chain reaction using the consensus primers MY11/MY09 and KS1/KS2, respectively. Results: HPV DNA was detected in 31/31 penile lesions. HPV DNA was also detected in 18/31 (58%) female genital brushings and 17/31 (54%) female oral brushings. HHV‐8 DNA was detected in 1/31 (3.2%) male genital brushings and 3/31 (9.6%) female oral mucosa brushings. None of the female genital brushings were HHV‐8 DNA‐infected. Conclusions: Based upon the results of this study, co‐infection between HPV and HHV‐8 in malignant and pre‐malignant penile lesions is an unlikely finding.     

Socioeconomic inequalities in the distribution of dental caries in Brazilian preschool children

Objectives: This study assessed the inequality in caries distribution and the association between socioeconomic indicators and caries experience of preschool children in a city in Brazil. Methods: A cross‐sectional study in a multistage random sample of 455, 1‐5‐year‐old children was conducted on National Children's Vaccination Day in Santa Maria, Brazil. Calibrated examiners evaluated the prevalence of dental caries and parents provided information about several socioeconomic indicators by means of a semi‐structured questionnaire. Data were analyzed using Poisson regression model. Results: The caries prevalence was 23.5 percent and the means for the decayed, missing and filled primary teeth was 0.8. A high inequality in the caries distribution with Gini coefficient of 0.8 and Significant Caries Index of 2.8 was observed. The oldest children, non‐white, with mothers having low level of education and from low household income had the highest prevalence of dental caries. Conclusion: Socioeconomic factors are strong predictors for the inequality in caries distribution in Brazilian preschool children.     

Menopause symptoms in women infected with HIV: Prevalence and associated factors

Objective. To evaluate the prevalence and factors associated with menopause symptoms in HIV-infected women.

Methods. A cross-sectional study of two groups of women was conducted: 96 with HIV and 155 without HIV. Women aged 40 years or older, non-users of hormone therapy in the last 6 months and native Brazilians were included. The prevalence of menopause symptoms was calculated according to the studied variables. Symptoms were grouped into six categories: vasomotor, psychological, genitourinary, weight gain, palpitations and insomnia. The generalized estimating equation model was applied to identify the factors associated with menopause symptoms in all women and for HIV-infected women only.

Results. The mean (±standard deviation) age of women with and without HIV was 48.9 ± 7.4 and 51.0 ± 8.7 years (p = 0.07), respectively. The median age at menopause for HIV-infected women was 47.5 years. Menopause symptoms were more frequent in HIV-infected women, highlighting psychological and vasomotor symptoms. HIV infection was associated with menopause symptoms (odds ratio (OR) = 1.65, p = 0.03), as well as age ranging from 45 to 54 years (OR = 1.77, p = 0.01), higher parity (OR = 2.38, p = 0.01) and self-perception of health as fair/poor (OR = 2.07, p < 0.01). Among HIV-infected women, the likelihood of presenting symptoms decreased in those aged 55 or older (OR = 0.16, p = 0.03) and increased in retired women (OR = 2.61, p = 0.02).

Conclusion. Menopause symptoms were common in HIV-infected women. HIV infection was independently associated with menopause symptoms, whereas age and being retired were associated with the occurrence of these symptoms in HIV-infected women.     

Emissions from residential combustion sector: how to build a high spatially resolved inventory

Usually, annual emission data from residential combustion sector are spatially disaggregated by population density to the sub-municipality spatial level. The aim of the present work is to define a methodological approach to develop/build a high-resolution emission inventory from residential combustion following a bottom–up approach. The conceptual model considers different approaches by fuel category (solid fuel–wood, gas and liquid fuels) according to distinct spatial coverage and type of activity data available for each category. For solid fuels, detailed activity data (per district), disaggregated according to the number and type of equipment, burned wood species and consumption rate, as well as specific emission factors (per wood species) were used. With regard to the gas and liquid fuels, the total national emission by fuel type using national consumption data and broader emission factors was disaggregated to the sub-municipality scale based only on the number of heating equipment. The choice of these disaggregation factors was influenced by both data availability and relevance. The results of the new disaggregated emission data have been compared with emission values resulting from the classical top–down approach using census/population data. The selected case study is Portugal. The results pointed out that major differences exist when comparing both approaches, namely regarding the spatial distribution/allocation of emissions. In the new approach, emissions are more redistributed over the territory, while in the old distribution, emissions are concentrated in the coastal urban areas (with hotspots in the main urban areas of Porto and Lisbon).     

Is Surgical Root Coverage Effective for the Treatment of Cervical Dentin Hypersensitivity? A Systematic Review

Background: Cervical dentin hypersensitivity (CDH) is characterized by tooth pain arising from root exposure. The aim of the present systematic review is to survey the literature on the efficacy of surgical root coverage techniques at reducing CDH in cases of gingival recession. Methods: An online electronic search was performed in PubMed, Web of Science, and Cochrane Library databases. Randomized clinical trials dating from the inception of the respective databases through November 2011 were selected. Studies addressing clinical parameters of periodontal plastic surgery outcomes and variables related to CDH in patients ≥18 years of age were included. The studies were evaluated by two independent reviewers. For each article, methodologic quality, size effect, the periodontal parameters measured, study design, methods, and results were analyzed. Results: Nine relevant articles were analyzed in the present review. A decrease in CDH was observed after periodontal surgery for root coverage. The risk of bias was considered low in two studies, and the size effect was considered large in one study. Conclusions: There is not enough scientific evidence to conclude that surgical root coverage procedures predictably reduce CDH. Well‐conducted clinical trials are needed to establish scientific evidence that allows periodontists to indicate root coverage as treatment for CDH.