钙黏蛋白E、桥粒芯糖蛋白2、磷酸化Akt和转录因子Snail在侵袭性前列腺癌中的作用

目的  探讨钙黏蛋白E（E-cadherin）、桥粒芯糖蛋白（2DSG2）、磷酸化Akt（pAkt）和转录因子Snail在前列腺癌发病进程中的作用;阐明E-cadherin、DSG2、pAkt和Snail对前列腺癌发病进程的影响及在临床预后中的作用。方法  组织芯片法检测E-cadherin、DSG2、pAkt和Snail在前列腺癌组织中的表达情况。结果  前列腺癌组织中E-cadherin表达明显低于正常前列腺组织,细胞质和细胞核中均可观察到pAkt表达,且在肿瘤高分化区和低分化区内均有较高表达。E-cadherin和DSG2表达呈显著正相关。E-cadherin和DSG2表达下降提示前列腺癌患者血清前列腺特异性抗原（PSA）浓度含量增加,Gleason评分较高及病理分期更高。结论  前列腺癌低分化细胞中钙黏蛋白表达降低,钙黏蛋白低表达提示肿瘤细胞侵袭性更强,转移发生率高。钙黏蛋白在前列腺癌发展中发挥关键作用,E-cadherin和DSG2有望成为侵袭性前列腺癌的预后因子。

     

复发性抑郁障碍患者尿酸水平及其相关因素分析

目的:探讨复发性抑郁障碍患者外周血清尿酸水平,及与临床特征的相关性。方法:对128例复发性抑郁障碍患者(复发抑郁障碍组)、120例首发抑郁障碍患者(首发抑郁障碍组)和252例健康对照者(正常对照组)行血清尿酸检测,同时采用9条目患者健康问卷(patient health questionnaire-9,PHQ-9)、广泛性焦虑障碍量表(generalized anxiety disorder-7,GAD-7)评定复发抑郁障碍组和首发抑郁障碍组抑郁焦虑症状的严重程度。结果:①多重线性回归分析提示,复发抑郁障碍组(β=-0.327,P<0.001)和首发抑郁障碍组(β=-0.154,P<0.001)血清尿酸水平低于正常对照组,复发抑郁障碍组(β=-0.169,P<0.001)血清尿酸水平低于首发抑郁障碍组,差异均有统计学意义。②复发抑郁障碍组血清尿酸水平与本次发作时间呈负相关(r=-0.242,P<0.001)。结论:抗氧化性能的下降可能与抑郁障碍有关,复发性抑郁障碍患者的抗氧化水平显著降低,且与本次发作时间相关。     

基于网络药理学和生物信息学研究苍附导痰丸治疗多囊卵巢综合征的分子机制＊

目的 探讨苍附导痰丸治疗多囊卵巢综合征（Polycystic ovary syndrome，PCOS）的潜在机制。方法 通过检索中药系统药理学数据库和分析平台（Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform，TCMSP）中苍附导痰丸组成药物的成分数据，筛选其有效成分和作用靶点；检索Drug Bank、GeneCards、OMIM、PharmGKB及Uniprot疾病数据库中PCOS相关靶点；检索GEO数据库，选择多囊卵巢综合征的差异表达基因。构建苍附导痰丸和PCOS成分、靶点间的相互关系网络，利用筛选出的关键靶点，构建蛋白互作（Protein-protein interaction，PPI）网络；在GO功能和KEGG信号通路富集的基础上，得出苍附导痰丸治疗PCOS的作用机制，进一步用分子对接技术验证靶点之间结合的可靠性。结果 共筛选出活性成分单体46个，159个苍附导痰丸治疗PCOS的共同靶点。结论 苍附导痰丸治疗PCOS具有多成分、多靶点、多通路的特点，推测苍附导痰丸可能通过调节炎症微环境从而改善肥胖型PCOS患者胰岛素抵抗，通过调节雌激素信号通路改善卵巢功能，因而可以改善肥胖及卵巢排卵的临床症状，为后期研究苍附导痰丸治疗PCOS的作用机制提供了新的理论支撑。     

高弹力无纺布衬垫在气压止血带中的应用效果

目的 探讨高弹力无纺布衬垫在气压止血带应用中的临床效果.方法 取80例四肢骨折患者,将其随机分为两组.对照组用普通绷带做气压止血带的衬垫;实验组则直接在肢体上套一层高弹力无纺布衬垫,然后环绕气压止血带;比较两组临床应用效果.结果 总有效率实验组为97.5％,对照组为90.0％;医生对手术中止血效果及衬垫满意率实验组为95.0％,对照组为85.0％;皮肤反应发生率实验组为2.5％,对照组为10.0％;两组比较,均P＜ 0.05,差异均具有统计学意义.结论 四肢手术中气压止血带使用率较高,高弹力无纺布衬垫比普通绷带衬垫效果好,具有无压痕、无皱折、不滑脱、不松动、高弹力等优点,值得推广使用.     

A Placebo-Controlled Trial of Dextromethorphan as an Adjunct in Opioid-Dependent Patients Undergoing Methadone Maintenance Treatment

Background:

Low-dose dextromethorphan (DM) might have anti-inflammatory and neurotrophic effects mechanistically remote from an NMDA receptor. In a randomized, double-blind, controlled 12 week study, we investigated whether add-on dextromethorphan reduced cytokine levels and benefitted opioid-dependent patients undergoing methadone maintenance therapy (MMT).

Methods:

Patients were randomly assigned to a group: DM60 (60mg/day dextromethorphan; n = 65), DM120 (120mg/day dextromethorphan; n = 65), or placebo (n = 66). Primary outcomes were the methadone dose required, plasma morphine level, and retention in treatment. Plasma tumor necrosis factor (TNF)-α, C-reactive protein, interleukin (IL)-6, IL-8, transforming growth factor–β1, and brain-derived neurotrophic factor (BDNF) levels were examined during weeks 0, 1, 4, 8, and 12. Multiple linear regressions with generalized estimating equation methods were used to examine the therapeutic effect.

Results:

After 12 weeks, the DM60 group had significantly longer treatment retention and lower plasma morphine levels than did the placebo group. Plasma TNF-α was significantly decreased in the DM60 group compared to the placebo group. However, changes in plasma cytokine levels, BDNF levels, and the methadone dose required in the three groups were not significantly different.

Conclusions:

We provide evidence—decreased concomitant heroin use—of low-dose add-on DM’s efficacy for treating opioid-dependent patients undergoing MMT.     

Solvent-assisted thermal reduction of microcrystalline graphene oxide with excellent microwave absorption performance

To prepare a new kind of electromagnetic wave absorber, and improve the processing technology and accessional value of natural microcrystalline graphite minerals (NMGMs), reduced microcrystalline graphene oxide (rGO-M), a novel absorber with high absorption, low reflection and a wide absorption band, was prepared from NMGMs using a solvent-assisted thermal reduction method. Moreover, the as-produced rGO-M with adjustable electrical resistivity can be easily transferred into well distributed bulk materials by freeze-drying technology. These unique structures and compositions make a great contribution to the impedance match, and cause strong conductive loss and various dipole polarization effects which greatly enhance the absorption. Meanwhile, the effective bandwidths below −5 dB and −10 dB are 11.7 GHz and 3.32 GHz respectively, and the reflection loss can reach −42.68 dB. The study will be beneficial to the development of carbon resources and carbon materials research. Besides, it can provide a scientific basis for the further improvement of the comprehensive utilization and the level of deep processing technology of NMGM resources.

Reduced microcrystalline graphene oxide (rGO-M), a novel absorber with high absorption, low reflection and a wide absorption band, was prepared from NMGMs using a solvent-assisted thermal reduction method.     

Design,synthesis and pharmacological evaluation of novel 2-chloro-3-(1H-benzo[d]imidazol-2-yl)quinoline derivatives as antitumor agents: in vitro and in vivo antitumor activity,cell cycle arrest and apoptotic response

A series of novel 2-chloro-3-(1H-benzo[d]imidazol-2-yl)quinoline derivatives (3a₁−3d₆) were designed and synthesized as antitumor agents. In vitro antitumor assay results showed that some compounds exhibited moderate to high inhibitory activities against HepG2, SK-OV-3, NCI-H460 and BEL-7404 tumor cell lines, and most compounds exhibited much lower cytotoxicities against HL-7702 normal cell line compared to 5-FU and cisplatin. In vivo antitumor assay results showed that the representative compound 3a₁ exhibited effective inhibition on tumor growth in the HepG2 xenograft mouse model. Mechanistic studies suggested that 3a₁ may exert antitumor activity by the up-regulation of Bax, intracellular Ca²⁺ release, ROS generation, p21, p27 and p53, downregulation of Bcl-2, activation of caspase-9 and caspase-3 and subsequent cleavage of PARP, and inhibition of CDK activity.

A series of novel 2-chloro-3-(1H-benzo[d]imidazol-2-yl)quinoline derivatives were designed and synthesized as antitumor agents under the combination principle. The antitumor activity and mechanisms were then evaluated.     

前列腺癌患者血清甲状旁腺相关蛋白测定的意义

目的观察前列腺癌患者血清甲状旁腺相关蛋白（ＰＴＨｒＰ）与病情及前列腺特异抗原（ＰＳＡ）的关系。方法采用免疫放射法（ＩＲＭＡ）和放射免疫法（ＲＩＡ）测定３１例前列腺癌患者血清ＰＴＨｒＰ、ＰＳＡ和钙离子，并以５０例前列腺增生症（ＢＰＨ）、５５例健康人和５７例其它恶性肿瘤患者作对照。结果前列腺癌组血清ＰＴＨｒＰ（４．６±４．４ｐｍｏｌ／Ｌ）和ＰＳＡ（８０．０±５２．６μｇ／Ｌ）明显高于ＢＰＨ和健康组（均为Ｐ＜０．０１）。３１例前列腺癌患者中１３例（４１．６％）血清ＰＴＨｒＰ增高，这１３例均属Ｄ期，其血清ＰＴＨｒＰ水平明显高于Ａ期（Ｐ＜０．０１）。２１例前列腺癌伴发高钙血症（ＨＨＭ）者血清ＰＴＨｒＰ与ＰＳＡ呈正相关关系（ｒ＝０．８２３，Ｐ＜０．００１）。ＢＰＨ组无１例血清ＰＴＨｒＰ增高。结论血清ＰＴＨｒＰ与前列腺癌的病程进展有关，其含量增高表明肿瘤已属晚期     

Mental Health Multimorbidity among Caregivers of Older Adults During the COVID-19 Epidemic

ObjectiveTo investigate mental health status and associated factors among caregivers of older adults during the COVID-19 epidemic in China.MethodsFrom March 1 to 31, 2020, 916 caregivers of older adults participated in an online cross-sectional survey on the prevalence of anxiety, depression, and sleep problems. The seven-item Generalized Anxiety Disorder Scale (GAD-7) was administered to measure anxiety symptoms, the two-item Patient Health Questionnaire (PHQ-2) was used to assess depressive symptoms, and a self-developed questionnaire was used to assess sleep quality and duration. Six questions about COVID-19-related experiences were used to assess community-level infection contact and the level of exposure to media information. The prevalence rates of anxiety, depression and sleep problems were computed. The Wald χ² were applied to compare the differences between subgroups. Multiple logistic regression analyses were performed to investigate factors associated with anxiety, depression, sleep problems, and multimorbidity.ResultsThe prevalence rates of anxiety, depression, and sleep problems were 46.8%, 29.8%, and 10.8%, respectively. Approximately 263 participants (28.7%) presented with two or more mental health problems. Being female (OR, 2.254; 95% CI, 1.510–3.363), having community-level COVID-19 contact (OR, 1.856; 95% CI, 1.189–2.898), and having a mental disorder (OR, 3.610; 95% CI, 1.644–7.930) were associated with increased risk of multimorbidity among caregivers. Caregivers who preferred positive information (OR, 0.652; 95% CI, 0.472–0.899) had reduced risk of multimorbidity.ConclusionAnxiety and depression were common among caregivers of older adults during the COVID-19 epidemic. Being female and having community-level COVID-19 contact were independent risk factors for experiencing multiple mental health problems. Preexisting mental disorders increased the risk of multimorbidity among caregivers, while enhanced access to positive media information decreased the risk of multimorbidity.     

Clinical and genetic analysis of six Chinese children with Poirier-Bienvenu neurodevelopmental syndrome caused by CSNK2B mutation

neurogenetics - Mutations in CSNK2B lead to Poirier-Bienvenu neurodevelopmental syndrome (POBINDS), a rare neurodevelopmental disorder. Only 14 cases of POBINDS have been reported worldwide. The...