P-gp、p53和VEGF在非小细胞肺癌中的表达及临床意义

目的:探讨P-糖蛋白(P-gp)、p53和血管内皮生长因子(VEGF)在非小细胞肺癌(N SCLC)组织中的表达及临床意义。方法:应用免疫组化检测84例(化疗组40例和非化疗组44例)N SCLC中P-gp、p53和VEGF的表达水平,40例癌旁组织作为对照组,比较三者的表达与生存率的关系。结果:P-gp、p53和VEGF在N SCLC中的阳性表达率分别为40.5%、70.23%和67.9%;P-gp与VEGF呈正相关(χ2=10.805,r=0.360,P=0.001),P-gp与p53无相关性(χ2=138,r=-0.047,P=0.637),p53与VEGF呈正相关(χ2=9.288,r=0.333,P=0.002);化疗组中P-gp、p53和VEGF表达阳性者生存时间明显低于阴性者(P<0.05);Cox显示:临床分期、P-gp、p53和VEGF与N SCLC的预后有关(P<0.05)。结论:P-gp、p53和VEGF检测对肺癌患者化疗效果、预后的判断具有的指导作用。     

亲子鉴定在计划生育工作中的应用分析

目的:探讨同步分型STR位点测试在计划生育工作中亲子鉴定中的应用及意义。方法:采用同步扩增、同步电泳分离,同步银染、同步多个短串联重复序列(STR)分型DNA分析方法进行亲子鉴定。结果:在30例三联体亲子鉴定案例中,排除亲生关系11例,占36.67%。16个位点的累计非父排除率0.99999878,平均匹配概率4.30×10-18。结论:同步分型STR位点的测试简便、快速,所得的结果准确可靠,重复性强。它不但可为司法机关解决相关案件提供有力证据,还可开展为计划生育工作提供法律依据。     

信息技术与中药学课程整合之我见

为适应教学需要，更好地促进教与学，达到更深意义的教学互动、教学互长，笔者将信息技术与中药学课程相整合，其体会如下。     

血府逐瘀汤治疗髋关节置换术后非感染性发热

[目的]探讨髋关节置换术后非感染性发热的防治。[方法]应用血府逐瘀汤加减辨证论治。[结果]43例患者体温恢复正常,其中36例在3天内体温恢复正常。[结论]该方法对髋关节置换术后非感染性发热有较好作用。     

胃灵颗粒治疗消化性溃疡的临床与实验研究

目的:研究胃灵颗粒对消化性溃疡愈合质量(QOUH)和大鼠乙酸慢性胃溃疡愈合质量的影响.方法:用随机分组的方法,观察临床消化性溃疡的复发率并用苏木精-伊红染色对大鼠愈合性胃溃疡再生黏膜进行定量观察,测定胃壁结合黏液量、胃酸、胃蛋白酶活性.结果:胃灵颗粒组能降低消化性溃疡的复发率,改善胃溃疡大鼠胃黏膜的组织损伤并提高胃壁结合黏液量,减少胃酸的排出.结论:胃灵颗粒能提高QOUH,是临床抗消化性溃疡复发的可能机制之一.     

Inhibition of stromal‐interacting molecule 1‐mediated store‐operated Ca2+ entry as a novel strategy for the treatment of acquired imatinib‐resistant gastrointestinal stromal tumors

Imatinib has revolutionized the treatment of gastrointestinal stromal tumors (GIST); however, primary and secondary resistance to imatinib is still a major cause of treatment failure. Multiple mechanisms are involved in this progression. In the present study, we reported a novel mechanism for the acquired resistance to imatinib, which was induced by enhanced Ca²⁺ influx via stromal‐interacting molecule 1 (STIM1)‐mediated store‐operated Ca²⁺ entry (SOCE). We found that the STIM1 expression level was related to the acquired resistance to imatinib in our studied cohort. The function of STIM1 in imatinib‐resistant GIST cells was also confirmed both in vivo and in vitro. The results showed that STIM1 overexpression contributed to SOCE and drug response in imatinib‐sensitive GIST cells. Blockage of SOCE by STIM1 knockdown suppressed the proliferation of imatinib‐resistant GIST cell lines and xenografts. In addition, STIM1‐mediated SOCE exerted an antiapoptotic effect via the MEK/ERK pathway. The results from this study provide a basis for further research into potential novel therapeutic strategies in acquired imatinib‐resistant GIST.     

近红外染料在宫颈癌早期诊断中的应用

目的:探讨近红外荧光染料在宫颈癌早期临床诊断中的应用价值。方法:选取我院2013年4月-2015年4月期间内收治的宫颈疾病患者100例,对其临床病理资料和早期诊断结果进行回顾性分析,以病理学诊断为金标准,对比分析近红外荧光染料吲哚菁绿(ICG)和阴道镜这两种检查方式对宫颈上皮内瘤样病变(CIN)、宫颈癌及炎症的检测符合率。结果:经病理学检查证实,所有宫颈疾病患者中早期宫颈癌患者30例,其中腺癌4例、鳞状细胞癌26例,CIN 20例,其中CIN I级4例、CIN II级7例、CIN III级6例、原位癌3例,宫颈炎症50例;ICG检查对早期宫颈瘤、CIN、宫颈炎症的诊断符合率分别为100%、95%、96%,均高于阴道镜。二者的综合诊断符合率比较,差异具有统计学意义(P<0.05)。结论:应用近红外染料荧光学检测对宫颈疾病患者进行诊断,可以提高早期宫颈癌及癌前病变的诊断准确率,具有较好的应用前景。     

大肠癌患者心理状况及应对方式对生存质量的影响

目的：探讨大肠癌患者不同的应对方式、心理状况对生存质量的影响,为采取相应的措施而提供科学依据。方法：于2014年11－2015年11月对某两所医院确诊住院的248例大肠癌患者应用癌症患者生存质量量表、医院焦虑抑郁量表评价及简单应对方式问卷进行调查评价。结果：大肠癌患者的生存质量各维度均处于中等偏下的水平;男性、居住于城市及经商的患者生存质量高于女性、居住于农村及其他职业的患者;无配偶只在躯体维度评分低于有配偶的,差异有统计学意义（P＜0．05）;采取积极应对方式的大肠癌患者的生存质量高于消极应对方式的患者,差异有统计学意义（P＜0．05）;消极应对方式和焦虑、抑郁是大肠癌患者生存质量的影响因素,差异有统计学意义（P＜0．05）。消极应对方式、焦虑、抑郁与QOL－LC总分呈负相关;积极应对方式与QOL－LC总分呈正相关。结论：医护人员应该关注患者的心理状态,鼓励其积极面对疾病,从而提高患者的生存质量。     

Utility of BRAF protein overexpression in predicting the metastasis potential of papillary thyroid carcinoma

V-raf murine sarcoma viral oncogene homolog B1 (BRAF) is a significant member of the MAPK pathway, the point mutation (V600E) of which is a common genetic event in papillary thyroid carcinoma (PTC). Investigators showed that the variations in BRAF expression levels were independent of the V600E mutation. These variations were involved in the pathogenesis of thyroid carcinomas. This study evaluated the feasibility of BRAF, proliferating cell nuclear antigen (PCNA) and hMSH2 as markers for the prediction of the metastatic potential of PTC. Using immunohistochemistry, the expression of BRAF, PCNA and hMSH2 proteins was studied in 70 PTC and 29 nodular goiter (NG) tissues. The results indicated that i) the positive rate of BRAF, PCNA and hMSH2 expression in PTCs was significantly higher than that in NGs (P=0.000, P=0.000 and P=0.003, respectively), ii) the positive rate of BRAF expression in the lymph node metastasis (LNM) group was significantly higher than that in the non-LNM group (P=0.019), iii) the age at diagnosis of PTC patients with LNM was significantly older compared to that without LNM (P=0.021) and iv) the positive rate of BRAF expression significantly correlated with that of PCNA and hMSH2 expression (P=0.000 and P=0.019, respectively). In conclusion, BRAF, PCNA and hMSH2 overexpression appeared to be molecular events of PTC carcinogenesis. Older patients with BRAF overexpression appear to be a high-risk group for PTC metastasis. Detection of BRAF expression is likely to aid in the prediction of the metastatic potential of the carcinoma.     

益母草水提物对异丙肾上腺素致大鼠心肌重构的影响

目的:从心肌组织胶原表达的角度观察益母草水提物对实验性大鼠心肌重构的影响。方法:SD大鼠皮下注射异丙肾上腺素建立心肌重构模型,随机分为正常对照组、模型组、依那普利组及益母草水提物低、高剂量组,药物干预15 w。检测大鼠心脏血流动力学指标、心输出量、心脏质量系数、左室质量系数、心肌羟脯氨酸含量、心肌组织Ⅰ、Ⅲ型胶原含量。结果:与模型组比较,益母草水提物高剂量能改善模型大鼠LVSP、+dp/dtmax和CO(P<0.05),益母草低剂量能改善-dp/dtmax、心脏质量系数和左室质量系数、心肌组织羟脯氨酸含量及Ⅰ、Ⅲ型胶原含量、Ⅰ/Ⅲ型胶原比(P<0.05)。结论:高剂量益母草水提物能改善ISO致大鼠心肌重构模型心脏收缩功能;低剂量益母草水提物能改善ISO致大鼠心肌重构模型舒张功能,下调胶原表达,改善心肌胶原构成比,减轻心肌重构程度。