苦瓜提取物对实验性Ⅱ型糖尿病大鼠的血糖的影响

目的:观察苦瓜提取物对链脲霉素引起的Ⅱ型糖尿病大鼠模型的血糖、血清胰岛素及血脂的影响。方法:大鼠尾静脉注射小剂量链脲霉素25mg·kg-1,同时喂以高糖高脂饲料复制Ⅱ型糖尿病大鼠模型.然后给予苦瓜提取物14,28,56mg·kg-1,连续灌胃给药4周后,测定血糖、血清胰岛素及血脂。结果:苦瓜提取物14,28,56mg·kg-1能降低实验性Ⅱ型糖尿病大鼠血糖及血清胰岛素水平,能升高HDL-C含量,而对血脂其它指标无明显影响。结论:苦瓜提取物对Ⅱ型糖尿病大鼠有治疗作用。     

IL-17 Eliminates the Therapeutic Effects of Myelin Basic Protein-Induced Nasal Tolerance in Experimental Autoimmune Encephalomyelitis by Activating IL-6

Interleukin (IL)-17 is a proinflammatory cytokine primarily secreted by Th17 cells, which are a CD4⁺ T-cell subset. Th17 cells and IL-17 are important in the pathogenesis of multiple sclerosis and in its established animal model, experimental autoimmune encephalomyelitis (EAE). However, it is unclear whether IL-17 contributes to EAE immune tolerance. We used the myelin basic protein (MBP) peptide MBP 68–86 to induce nasal tolerance to EAE, and simultaneously interfered with the tolerance by treatment with different doses of IL-17. We found that IL-17 dramatically interfered with MBP 68–86-induced immune tolerance. IL-17 administration increased IL-6 release, skewing T cell differentiation towards Th17 cells and decreasing the number of Treg cells. This led to an imbalance between Treg cells and Th17 cells and spurred the development of EAE.     

Ketamine inhibits LPS-induced calcium elevation and NF-kappa B activation in monocytes

Objective:To investigate whether ketamine could inhibit lipopolysaccharide (LPS)-induced intracellular calcium elevation and NF-kappa B activation in monocytes. Materials and methods:Isolated rat monocytes were challenged with 10 g/ml LPS with or without the presence of various concentrations of ketamine (10, 100, 1000 M). Intracellular calcium was monitored by laser confocal microscopy. NF-kappa B activity of the nuclear extracts of monocytes was analyzed by electrophoretic mobility shift assay (EMSA). Results:LPS provoked a significant calcium elevation and enhanced NF-kappa B activity in monocytes. Ketamine above concentration of 100 M inhibited endotoxin-induced intracellular calcium elevation and NF-kappa B activity. Ketamine itself had no effect on either of them. Conclusions:These findings suggest that ketamine could suppress NF-kappa B in monocytes exposed to endotoxin, and this anti-inflammatory effect might act through attenuating intracellular calcium elevation.Received 31 October 2003; returned for revision 18 December 2003; accepted by I. Ahnfelt-Rønne 26 Januaryy 2004     

Pyriform sinus fistula in children: A comparison of endoscopic-assisted surgery and endoscopic radiofrequency ablation

BackgroundPyriform sinus fistula (PSF) is a rare congenital anomaly, and the preferred definitive treatment is yet to be verified. In this study, we investigated the treatment outcomes of PSF specifically comparing endoscopic-assisted surgery and endoscopic radiofrequency ablation (RA).MethodsThe medical records of patients treated for PSF at the Shanghai Children's Hospital between October 2016 and September 2019 were retrospectively evaluated.ResultsThere were 93 girls and 98 boys. The median age at onset and operation was 3 years and 5 years, respectively. Endoscopic-assisted surgery was performed in 143 patients. During the same period, RA was performed in 48 patients, and 10 of them concurrently underwent incision and drainage of neck abscesses. Longer hospital stay was found in the endoscopic-assisted surgery group than in the RA group (10.50 ± 3.93 vs. 5.02 ± 3.30 days, P < 0.001). Postoperative complications were not significantly different between the two groups, except for neck infection (0 vs. 8.3%, P = 0.004). After a median follow-up period of 21 months, no significant difference was found between the two groups in terms of recurrence (1.4% vs. 0, P = 0.560).ConclusionPatients treated with RA had a significantly shorter hospital stay than those treated with endoscopic-assisted surgery. Outcomes of endoscopic-assisted surgery and RA were not significantly different for the management of PSF and treatment method should be tailored to the patient.Level of evidenceIV.     

不育男性精浆转铁蛋白含量与精子顶体形态的关系

目的 :探讨不育男性精浆转铁蛋白 (Tf)含量与精子顶体完整性的关系。方法 :149例不育男性患者按精子密度≥ 2 0× 10 6 / ml和 <2 0× 10 6 / ml分正常精子密度组和少精子组 ,同时进行精浆 Tf和精子顶体完整率 (PIA)分析。结果 :不育男性少精子组精浆 Tf含量显著低于正常精子密度组 (P<0 .0 1) ;正常精子密度的不育男性精子 PIA<80 %组精浆 Tf含量显著低于精子 PIA≥ 80 %组 (P<0 .0 1)。结论 :正常精子密度的不育男性精子 PIA下降与精浆 Tf水平有密切的关系     

氟伐他汀治疗肾病综合征高脂血症的量效观察

目的　观察氟伐他汀对肾病综合征高脂血症剂量与疗效的关系。方法　在常规治疗的基础上 ,6 4例肾病综合征高脂血症患者分为 2组 ,A组 33例口服氟伐他汀 80mg/d ,B组 31例口服氟伐他汀 2 0mg/d ,观察疗效和安全性。结果　A组服药 2周后 ,血清TC ,TG ,LDL -C水平与治疗前比较分别降低 2 4 .6 5 % ,2 5 .0 8% ,32 .0 3% (P <0 .0 0 1) ;B组分别降低4 .83% ,0 .33% ,5 .87% (P >0 .0 5 )。以上结果的组间比较均有统计学差别。A组服用 2周时降低TC ,TG ,LDL -C的显效率及降低TC总有效率均明显高于B组。两组治疗期间均未发现明显的不良反应。结论　氟伐他汀治疗肾病综合征高脂血症时 ,治疗效果与剂量存在明显的正相关 ,对肾病综合征高脂血症患者应采用大剂量氟伐他汀方法 ,对及时纠正高脂血症、改善病情有利     

注射用阿莫西林钠克拉维酸钾细菌内毒素检查

目的建立注射用阿莫西林钠克拉维酸钾细菌内毒素检查方法.方法按中国药典2000年版附录细菌内毒素检查法要求进行试验.结果注射用阿莫西林钠克拉维酸的浓度为2.5mg/ml时不干扰鲎试剂与细菌内毒素的凝胶反应.结论细菌内毒素检查法适用于检测注射用阿莫西林钠克拉维酸钾中的内毒素.     

Effects of histaminergic neuron on the developmental process of conditioned reward induced by morphine

To investigate the effect of histaminergic neuron on morphine - induced conditioned place preference （ CPP）, the model of CPP was used to assess the rewarding effect of morphine. Morphine （2, 5, 10 mg/kg） significantly produced a CPP in a dose-dependent manner. In addition, morphine simultaneously and markedly lessened the histaminergic neurons in the TM,