曼氏迭宫绦虫成虫与裂头蚴乳酸脱氢酶和酯酶同工酶的研究

本文应用聚丙烯酰胺凝胶电泳(PAGE)法分析了曼氏迭宫绦虫成虫与裂头蚴乳酸脱氢酶(LDH)和酯酶(EST)同工酶谱。结果表明,成虫LDH同工酶带和裂头蚴一样,仅有一条带,其位置差异不显著;成虫EST显示5条带,主带有2条;裂头蚴EST显示10条带,主带有3条。     

实时超声法测定空腹胃窦面积的临床意义

本文利用实时超声法测定了５８例健康人及５９例胃病及糖尿病病人空腹胃窦面积。结果显示，健康中国人胃窦面积为１．５２ｃｍ２±０．４３ｃｍ２，与性别、年龄、身高、体重无关，胃炎、糠尿病、活动期十二指肠溃疡病人空腹胃窦面积均有不同程度扩大，慢性胃炎伴呃逆，腹胀症状及糖尿病病人异常率为１００％。本文中有２２例病人同时做食管测压检测，结果显示溃疡病病人空腹胃窦面积与食道上、中、下段压力均无关，糖尿病、胃炎病人胃窦面积与ＬＥＳＰ呈负相关。此研究结果提示空腹胃窦面积可在某种程度上代表胃排空功能。     

Procoagulant activity of reversibly acylated human factor Xa

The plasma clotting factors used to treat hemophiliacs who have developed inhibitory antibodies have a shared history of limited clinical safety and utility. To improve on existing bypass factors, we have developed a reversibly acylated form of human plasma factor Xa capable of providing a time-dependent release of procoagulant activity. Factor Xa was treated with p-amidinophenyl p'-anisate to generate anisoyl Xa. The chemical modification of the protein involves acylation of the active site serine residue of factor Xa. Anisoyl Xa deacylated in a time, pH, and temperature-dependent manner. Active factor Xa generated on deacylation of anisoyl Xa exhibited amidolytic and prothrombinase complex activities in in vitro assays, the level being comparable to those of untreated factor Xa. When Anisoyl Xa was infused into rabbits, active factor Xa was generated on deacylation of the acylated enzyme, which shortened the activated partial thromboplastin time (APTT) in a dose-dependent manner. The duration of effect on rabbit APTT could be directly correlated to the level of human plasma factor Xa. Because anisoyl Xa bypasses the "tenase" complex that is compromised in hemophilia A and B and is unaffected by inhibitory antibodies, it has the potential to be used as an effective bypass therapy.     

骨转换指标和骨密度预测骨折危险性的可行性

背景：骨密度虽然可以诊断骨质疏松,但不能及时反映受试者正在发生的骨代谢情况;骨转换指标虽然不能诊断骨质疏松,但它可以及时反映受试个体正在发生的骨转换速率。 目的：概述骨转换指标和骨密度预测骨质疏松症的骨折危险性,从理论上分析,骨折术后愈合与否的预测方法的可行性。 方法：以bone tunrnover,biochemical marker,osteoporosis为检索词,检索Pubmed数据库(1999-01/2009-01);以骨转换,骨代谢,生化标志物,骨密度,骨质疏松,骨折为检索词,检索CNKI数据库(1999-01/2009-01)。文献检索语种限制为英文和中文。纳入与骨转换指标和骨密度预测骨折危险性的研究紧密相关的文章;排除重复文献。 结果与结论：计算机初检得到631篇文献,根据纳入排除标准,对其中31篇文献进行分析。骨质疏松症是老年人发病及死亡的主要原因之一,早期预测骨质疏松症的骨折危险性意义重大。文章介绍了长期制动后的骨代谢机制、骨生化标志物种类、骨密度测量、多细胞基本单位和 OPG-RANKL-RANK 系统在骨重建中的作用;重点介绍了国内外学者运用骨转换指标和骨密度预测骨质疏松症和长期制动后的骨折危险性,从理论上分析骨折术后愈合与否的预测方法的可行性。     

A precise and specific method for quick determinationof sulfur fumigation for moutan cortex

Objective: Establish a quick, precise and specific method to determine whether the moutan cortex obtained from market is processed with sulfur; provide a reliable method for the scientific evaluation.Methods: Three methods, including acid-base titration method, high-performance liquid-chromatography(HPLC) method, and ultraperformance liquid chromatography coupled with time-of-flight mass spectrometry(UHPLC-TOF-MS) method were used to detect whether30 batches of samples were fumigated with sulfur.Results: The results of three methods were substantially the same. Fifteen batches were identified to have been processed with sulfur fumigation, while others were not.Conclusions: The HPLC method was found to be most appropriate for the determination of sulfur fumigation for moutan cortex.     

BCL-2抑制剂联合低剂量阿糖胞苷治疗异基因造血干细胞移植后复发老年急性髓系白血病一例报告并文献复习

老年急性髓系白血病(AML)患者治疗困难,长期生存率低,预后差。文献报道,老年AML患者积极进行异基因造血干细胞移植(allo-HSCT)有助于改善远期生存[1]。但是,移植后复发患者通常难以耐受强化疗,很难再次获得完全缓解(CR)。近期我们采用BCL-2抑制剂联合小剂量阿糖胞苷成功治疗1例allo-HSCT后复发的老年AML患者。     

甲状腺相关眼病的影像学检查

甲状腺相关眼病(thyroid associated ophthalmopathy,TAO)是一种自身免疫性眼眶炎症性疾病,影像学检查是其重要的辅助检查手段。通过影像学检查能充分了解眶内病变,鉴别具有相似临床表现的眼眶疾病; 同时对TAO分期、辅助诊断压迫性视神经病变(compressive optic neuropathy,CON)也具有重要意义。     

颅脑外伤性迟发性出血的CT诊断

目的：用ＣＴ随访研究颅内迟发性血肿,方法：在２３２例颅脑外伤患者中行ＣＴ随访检查,结果：６１例发现有迟发性颅内出血,其中３８例为原血肿增大或出现新的血肿;８例为对冲伤血肿,１５例为脑挫伤与小量散在出血灶,结论：在外伤病例中,即使开初ＣＴ扫描为阴性者,如病人的临床表现有改变,如症状加重,意识丧失,抽搐等,应立即行ＣＴ复查,对ＣＴ随访的价值及迟发出血的机理也进行了讨论。     

妊娠期亲密伴侣暴力筛查及干预研究进展

综述妊娠期亲密伴侣暴力的筛查现状、筛查障碍因素、筛查工具以及干预措施,为今后采取措施预防和减少妊娠期亲密伴侣暴力、改善母婴健康结局提供参考。     

Visualization of suppressed thyroid tissue by Tc-99m MIBI.

The authors describe a case of suppressed normal thyroid tissue in a patient with autonomous toxic nodule, which was visualized by a Tc-99m MIBI thyroid scan without thyrotropin (TSH) stimulation. The findings suggest that Tc-99m MIBI can be a substitute for conventional thyroid imaging using I-131 to visualize suppressed normal thyroid tissue without TSH stimulation and its side effects.