Nutlin-3-induced redistribution of chromatin-bound IFI16 in human hepatocellular carcinoma cells in vitro is associated with p53 activation

Aim:

Interferon-γ inducible protein 16 (IFI16), a DNA sensor for DNA double-strand break (DSB), is expressed in most human hepatocellular carcinoma cell (HCC) lines. In this study we investigated the re-localization of chromatin-bound IFI16 by Nutlin-3, a DNA damage agent, in HCC cells in vitro, and the potential mechanisms.

Methods:

Human HCC SMMC-7721 (wild-type TP53), Huh-7 (mutant TP53), Hep3B (null TP53) and normal fetal liver L02 cell lines were examined. DSB damage in HCC cells was detected via γH2AX expression and foci formation assay. The expression of IFI16 and IFNB mRNA was measured using RT-PCR, and subcellular localization and expression of the IFI16 protein were detected using chromatin fractionation, Western blot analysis, and fluorescence microscopy.

Results:

Treatment of SMMC-7721 cells with Nutlin-3 (10 μmol/L) or etoposide (40 μmol/L) induced significant DSB damage. In SMMC-7721 cells, Nutlin-3 significantly increased the expression levels of IFI16 and IFNB mRNA, and partially redistributed chromatin-bound IFI16 protein to the cytoplasm. These effects were blocked by pretreatment with pifithrin-α, a p53 inhibitor. Furthermore, Nutlin-3 did not induce ectopic expression of IFI16 protein in Huh-7 and Hep3B cells. Moreover, the association of IFI16 with chromatin and Nutlin-3-induced changes in localization were not detected in L02 cells.

Conclusion:

Nutlin-3 regulates the subcellular localization of IFI16 in HCC cells in vitro in a p53-dependent manner.     

Down-regulation of miR-622 in gastric cancer promotes cellular invasion and tumor metastasis by targeting ING1 gene

AIM:To evaluate the biological and clinical characteristics of miR-622 in gastric cancer. METHODS:We analyzed the expression of miR-622 in 57 pair matched gastric neoplastic and adjacent non-neoplastic tissues by quantitative real-time polymerase chain reaction. Functional analysis of miR-622 expression was assessed in vitro in gastric cancer cell lines with miR-622 precursor and inhibitor. The roles of miR-622 in tumorigenesis and tumor metastasis were analyzed using a stable miR-622 expression plasmid in ...     

IL-37b gene transfer enhances the therapeutic efficacy of mesenchumal stromal cells in DSS-induced colitis mice

Aim:

To investigate whether the transfer of the IL-37b gene, a newly identified inhibitor of both innate and adaptive immunity, could improve the therapeutic efficacy of mesenchumal stromal cells (MSCs) in inflammatory bowel disease (IBD).

Methods:

The expression of IL-37 in biopsied specimens of the patients with active ulcerative colitis (UC) was detected using RT-PCR and immunohistochemistry. Mice were treated with 3% dextran sulfate sodium (DSS) for 8 days to induce colitis. Before DSS treatment, the mice were injected with MSCs, MSC-eGFP or MSC-IL37b. Their body weight was measured each day, and the colons and spleens were harvested on d 10 for pathological and biochemical analyses.

Results:

In biopsied specimens of the patients with active UC, the expression of IL-37 was dramatically elevated in inflamed mucosa, mainly in epithelial cells and infiltrating immune cells. Compared to MSC-eGFP or MSCs, MSC-IL37b administration significantly attenuated the body weight and colon length reduction, and decreased the histological score in DSS-induced colitis mice. Furthermore, MSC-IL37b administration increased the percentage of myeloid-derived suppressor cells (MDSCs) among total splenic mononuclear cells as well as the percentage of regulatory T cells (Tregs) among splenic CD4+ T cells in the mice. Moreover, MSC-IL37b administration increased the IL-2+ cells and decreased the IFN-γ+ cells among splenic CD4+ T cells.

Conclusion:

IL-37 is involved in the pathophysiology of UC. IL-37b gene transfer enhances the therapeutic efficacy of MSCs in DSS-induced colitis mice by inducing Tregs and MDSCs and regulating cytokine production.     

顾氏喉科温补脾肾-“碧雪散”治疗口腔扁平苔藓

杨义芳认为口腔扁平苔藓多由外感六淫、饮食不当、情志所伤、素体虚弱等引起,治疗一是善用温药以温补脾肾,补肾之精气,精气旺盛,正气充足,邪不可干;二是使用清热活血及祛风除湿之药,升提收毒上行,以渗利下导为务;三是配合特色外吹散剂-"碧雪散"缓解局部口腔黏膜红肿疼痛。附验案1例。     

2014年诺贝尔生理学或医学奖获得者的科学方法研究

在医学领域,科学研究经过了经验医学、观察医学和实验医学几个阶段,科学研究的主要方向是探索人类未知的世界,因此在进行科学研究时就要求我们要掌握科学的研究方法,针对特定的目的,特定的需要,策划出可行的方案,并且在此基础上进行一定的创新。这些对于医学领域的研究有着十分重要的意义,并因此涌现了多位做出卓越贡献的科学家。2014年诺贝尔生理学或医学奖由一位美国科学家和两位挪威科学家共同分享,他们各自的科学研究方法都独具特色。论文中主要分为三个部分,第一部分对三位获奖者做了一些介绍,包括他们的个人经历以及所获成就。第二部分注重就他们的研究历程和方法做出科学研究方法的分析。第三部分是对于全文的总结,是我从对获奖者分析中所获得的启发和个人的看法。     

Comparison of the EuroQol and short form 6D in Singapore multiethnic Asian knee osteoarthritis patients scheduled for total knee replacement

OBJECTIVE: To compare the EuroQol (EQ-5D) and Short Form 6D (SF-6D) among multiethnic Asian patients with knee osteoarthritis (OA) scheduled for total knee replacement in Singapore. METHODS: Patients were asked to complete questionnaires including the EQ-5D, Short Form 36, Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), and Lequesne knee index. EQ-5D and SF-6D utility scores were calculated using the scoring algorithms developed from the UK general population. Agreement between the 2 instruments was assessed by comparing their score distributions, means, medians, intraclass correlation coefficients (ICCs), and a Bland-Altman plot. Correlations of the EQ-5D and SF-6D with WOMAC and Lequesne knee index scores were also examined. RESULTS: A consecutive sample of 258 knee OA patients (127 English-speaking and 131 Chinese-speaking) participated. The mean +/- SD EQ-5D utility score was 0.49 +/- 0.31 (range -0.25-1.00) and the mean SF-6D utility score was 0.63 +/- 0.12 (range 0.32-0.89). In a hypothetical example, this 0.14-point difference in mean utility scores yielded a difference of $10,000/quality-adjusted life year (QALY) in cost-effectiveness ratios. The score distribution was bimodal for the EQ-5D and normal for the SF-6D. This poor agreement was also demonstrated by the Bland-Altman plot and the low ICC (range 0.18-0.54). Correlations of the WOMAC and Lequesne index with the EQ-5D were higher than with the SF-6D. CONCLUSION: Using different preference-based health-related quality of life instruments may yield different utility scores, which could have a great impact on QALY estimates. This highlights the importance of selecting appropriate instruments for economic evaluation. Additional research is needed to determine which instrument (the EQ-5D or the SF-6D) should be used in OA patients.     

肺癌中医护理方案对Ⅲ-Ⅳ期肺癌患者生活质量的影响

目的:观察综合性中医护理方案对Ⅲ-Ⅳ期肺癌患者生活质量的影响,为寻找有效的护理方案提供临床研究依据。方法:将80例Ⅲ-Ⅳ期肺癌住院患者按入院先后顺序随机分为治疗组和对照组各40例,分别采用综合性中医护理方案和常规护理方法进行护理后,运用肺癌的主要临床症状分度表评价症状疗效,运用卡氏评分(KPS)评价患者生活质量。结果:治疗组在护理后中医证候总积分高于对照组,差异有统计学意义(P<0.05),护理后卡氏评分试验组优于对照组(P<0.05)。结论:综合性中医护理方案可改善Ⅲ-Ⅳ期肺癌患者症状并提高患者的生活质量。     

赵玉庸教授治疗无症状IgA肾病临床经验研究

无症状IgA肾病患者临床多见,西医无特殊办法,中医治疗有一定优势,赵玉庸教授根据多年临床经验,认为IgA肾病主要病机为脾肾亏虚,瘀血阻络,采用病证结合方法辨证治疗,包括结合IgA肾病临床特点治疗、结合IgA肾病病理改变治疗、基础方结合实验室检查加减治疗等方面,疗效较好,故进行总结研究,为临床治疗此类患者提供一些思路和方法。     

心电图多指标诊断冠心病的意义

对３０例冠心病患者及２８例正常对照者进行心电图踏车运动试验，测量ＳＴ及非ＳＴ参数，结果显示：（１）各指标诊断冠心病的敏感性依次为：ΔＱ－Ｔｃ（８３．３％）、ΔＱ（７６．７％）、ΔＲＳＴ及ΔＪ－Ｔｃ（６６．７％）、ΔＲ（６３．３％）、ΣＲ（５６．７％）、ＳＴ（５０％）、Ｕ波倒置（３０％）；特异性依次为：Ｕ波倒置（１００％）、ΣＲ（８９．３％）、ＳＴ及ΔＪ－Ｔｃ（８２．１％）、ΔＲ（７８．６％）、ΔＲ