全文获取类型
收费全文 | 112642篇 |
免费 | 12293篇 |
国内免费 | 7886篇 |
专业分类
耳鼻咽喉 | 1108篇 |
儿科学 | 1106篇 |
妇产科学 | 925篇 |
基础医学 | 11337篇 |
口腔科学 | 2292篇 |
临床医学 | 14512篇 |
内科学 | 13976篇 |
皮肤病学 | 1033篇 |
神经病学 | 4954篇 |
特种医学 | 4410篇 |
外国民族医学 | 48篇 |
外科学 | 11922篇 |
综合类 | 24093篇 |
现状与发展 | 27篇 |
一般理论 | 12篇 |
预防医学 | 9058篇 |
眼科学 | 2917篇 |
药学 | 12564篇 |
167篇 | |
中国医学 | 8237篇 |
肿瘤学 | 8123篇 |
出版年
2024年 | 486篇 |
2023年 | 1796篇 |
2022年 | 4642篇 |
2021年 | 6471篇 |
2020年 | 5231篇 |
2019年 | 3867篇 |
2018年 | 3998篇 |
2017年 | 4078篇 |
2016年 | 3764篇 |
2015年 | 5799篇 |
2014年 | 7177篇 |
2013年 | 7058篇 |
2012年 | 10576篇 |
2011年 | 10959篇 |
2010年 | 7967篇 |
2009年 | 6531篇 |
2008年 | 7228篇 |
2007年 | 6631篇 |
2006年 | 5958篇 |
2005年 | 5069篇 |
2004年 | 3274篇 |
2003年 | 2770篇 |
2002年 | 2133篇 |
2001年 | 1673篇 |
2000年 | 1492篇 |
1999年 | 1341篇 |
1998年 | 739篇 |
1997年 | 708篇 |
1996年 | 584篇 |
1995年 | 492篇 |
1994年 | 407篇 |
1993年 | 266篇 |
1992年 | 263篇 |
1991年 | 229篇 |
1990年 | 225篇 |
1989年 | 168篇 |
1988年 | 155篇 |
1987年 | 133篇 |
1986年 | 112篇 |
1985年 | 90篇 |
1984年 | 56篇 |
1983年 | 41篇 |
1982年 | 22篇 |
1981年 | 20篇 |
1980年 | 18篇 |
1979年 | 20篇 |
1978年 | 20篇 |
1975年 | 11篇 |
1974年 | 11篇 |
1969年 | 9篇 |
排序方式: 共有10000条查询结果,搜索用时 218 毫秒
1.
2.
Bortezomib is a novel proteasome inhibitor, which has been successfully used to treat mantle cell lymphoma and multiple myeloma. However, the direct effects of bortezomib on acute promyelocytic leukaemia (APL) have not been fully investigated. In the present study, the WST-8 assay, western blotting, flow cytometry, monodansylcadaverine staining and transmission electron microscopy were performed. It was demonstrated that bortezomib treatment induced a time- and dose-dependent decrease in the viability of NB4 cells. Bortezomib treatment induced cell apoptosis in NB4 cells, as assessed by Annexin V/propidium iodide analysis, and the detection of cleaved caspase-3, cleaved poly(ADP-ribose) polymerase, Bax and Bcl-2 expression. Furthermore, bortezomib treatment induced autophagy in NB4 cells, as indicated by autophagosome formation, p62 degradation, LC3-I to LC3-II conversion and formation of acidic autophagic vacuoles. Notably, autophagy induced by bortezomib was initiated prior to apoptosis. Inhibition of autophagy by knocking down Beclin-1 expression increased bortezomib-induced apoptosis in NB4 cells. Therefore, the present study revealed that the combination of bortezomib and autophagy inhibition may be a potential treatment strategy for APL. 相似文献
3.
“三全育人”视角下思政教育机制实践与探索——“杏林成长导师”计划的构建 《医学教育管理》2021,7(6):656-660
“三全育人”是高校思政教育工作的关键一环,也是中医药高校推动思政教育的重要内容。以“三全育人”为视角对北京中医药大学思想政治教育举措“杏林成长导师”计划路径、内容深入分析,运用统计分析和文献研究方法,剖析该计划对中医学专业大学生的学业、思想和实践等多个层面的现实成效,从而为“三全育人”理念在中医药院校制度建构中的应用提供新的视角与方法。 相似文献
5.
Lei Yang You-Ling Shi Yan Ma Wei-Wei Ren Guang-Ming Pang Jiao Liu 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2022,130(1):43-52
Krüppel-like factor 16 (KLF16), a member of the Krüppel-like factor (KLF) family, has been extensively investigated in multiple cancer types. However, the role of KLF16 in oral squamous cell carcinoma (OSCC) remains unknown. Thus, we conducted this study to investigate its related mechanism. KLF16 expression in OSCC cell lines was quantified by western blotting. Then, OECM1 and OC3 cells were divided into Blank, siCtrl, siKLF16#1 and siKLF16#2 groups. Subsequently, cell proliferation was detected using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assays, cell migration and invasion were detected with wound healing and Transwell assays, and cell cycle distribution and cell apoptosis were detected via flow cytometry. KLF16, p21, CDK4, Cyclin D1 and p-Rb expression was detected by western blotting. Finally, xenograft models were established in nude mice to observe the in vivo effects of KLF16 on OSCC. KLF16 protein expression was upregulated in OSCC cells. Compared to the cells in the Blank group, the OECM1 and OC3 cells in the siKLF16#1 group and siKLF16#2 group exhibited a sharp decrease in proliferation but a remarkable increase in apoptosis. Moreover, the proportion of cells in the G0/G1 phase notably increased and that in the S phase decreased, with evident decreases in cell invasion and migration. Moreover, KLF16, cyclin-dependent kinase 4 (CDK4), Cyclin D1 and p-Rb protein expression was upregulated, but p21 expression was downregulated. The mice in the siKLF16#1 and siKLF16#2 xenograft model groups exhibited slower tumour growth and smaller tumours with evident downregulation of Ki67 expression compared to the mice in the Blank group. KLF16 expression was upregulated in OSCC cells, and interfering with KLF16 led to cell cycle arrest, inhibited OSCC cell growth and promoted cell apoptosis. 相似文献
6.
Clinical and Experimental Medicine - Recently, the use of novel targeted drugs significantly improved the overall response rate (ORR) and survival of patients with relapsed/refractory chronic... 相似文献
7.
8.
9.
目的通过Meta分析评价安宁疗护对社区临终患者生活质量的影响。方法计算机检索PubMed、CINAHL、ScienceDirect、MEDLINE、Cochrane Library、SAGE数据库、中国知网、维普中文科技期刊数据库、万方数据库和中国生物医学文献数据库中安宁疗护对社区临终患者的随机对照试验,由2名研究人员独立筛选文献并提取资料,对符合质量标准的随机对照试验进行Meta分析。结果共纳入7篇文献,包括1095名社区临终患者。Meta分析结果显示,安宁疗护可以改善社区临终患者的总体生活质量(SMD=0.48,95%CI0.07~0.88,P=0.02)和情绪功能(SMD=1.27,95%CI0.59~1.95,P<0.001),在改善生活质量的躯体功能、角色功能、认知功能和社会功能水平上差异无统计学意义(P>0.05)。结论安宁疗护能改善社区临终患者的总体生活质量和情绪功能,但对生活质量其他功能的改善情况还需要设计更加规范、多中心、大样本的随机对照试验进一步证实。 相似文献
10.