B组轮状病毒WH-2株vp7基因的原核表达与抗体的制备

目的：在大肠杆菌中表达人B组轮状病毒WH-2株VP7蛋白并制备其兔抗血清。方法：根据B组轮状病毒（GBRV）WH-2株vp7基因的全序列设计引物,用PCR的方法扩增得到vp7基因的编码区。将其克隆到原核GST融合表达载体pGEX-KG内,转化大肠杆菌E.coliDH5α,IPTG诱导表达人B组轮状病毒WH-2株VP7蛋白,经SDS-PAGE分离纯化表达的蛋白免疫新西兰兔,制备人B组轮状病毒WH-2株VP7蛋白抗血清。结果：经鉴定确认,vp7基因以正确的方式插入到载体中,此重组表达载体经IPTG诱导后,可表达相对分子量为53.4 kDa的GST-VP7融合蛋白。制备的抗血清经同样诱导表达的表达载体pGEX-KG表达产物吸收后1：500倍稀释后用Western Blot分析,与53.4 kDa的GST-VP7融合蛋白获得特异性显色信号。结论：人B组轮状病毒WH-2株VP7蛋白成功在大肠杆菌中GST融合表达,所表达的蛋白和制备的抗体不但为研究结构与功能提供了物质基础,也为GBRV所引起的疾病预防、诊断和治疗等流行病学研究和临床诊断奠定了基础,具有重要实际应用价值。     

原发性骨小细胞肿瘤的病理形态和免疫组化分析

目的：探讨免疫组化染色及病理形态在原发性骨小细胞肿瘤鉴别诊断中的意义。方法：对39例骨的小细胞肿瘤[其中尤因肉瘤/外周性原始神经外胚层肿瘤（EW/PNET瘤）22例、小细胞骨肉瘤6例、间叶性软骨肉瘤3例和恶性淋巴瘤8例]，观察其病理形态并作免疫组化分析，所用抗体O13、NSE、S-100、Actin和LCA。结果：EW/PNET瘤20/22例为O13阳性。NSE:EW/PNET瘤16/22例和1例骨肉瘤阳性。7例EW/PNET瘤、1例骨肉瘤及3例间叶性软骨肉瘤均有S-100阳性。所有恶性淋巴瘤的LCA均为阳性。骨肉瘤均见骨样基质，间叶性软骨肉瘤可见分化较好的软骨岛。结论：O13的染色对于EW/PNET瘤有诊断意义。小细胞骨肉瘤的成骨和间叶性软骨肉瘤的软骨分化为形态上的诊断要点。     

CD117阳性小肠间质瘤中Ki67和p53蛋白的表达及在预后中的意义

目的 :研究小肠间质瘤 (smallbowelstromaltumors,SBST)Ki6 7和p5 3免疫组织化学的表达 ,探讨两者在肿瘤良恶性划分和预后中的作用。方法 :选用CD117阳性的SBST 33例进行光镜观察 ,用EnVisionTM+二步法免疫组化方法检测Ki6 7和 p5 3蛋白在肿瘤中的表达情况并与形态学进行比较。 结果 :33例SBST患者 ,男 2 0例 ,女13例 ,年龄 2 1～ 71岁 ,发生于十二指肠 5例 ,空肠 2 2例 ,回肠 6例。肿瘤最大径的范围在 1.5～ 2 0cm之间。在光镜下出现肿瘤内坏死的有 11例。肿瘤核分裂象计数每 5 0个高倍镜视野 0个有 6例 ,1～ 2个 5例 ,≥ 3个的有 2 2例。 33例中良性 4例 ,交界性 4例 ,恶性 2 5例。随访时间为 3～ 180个月 (平均 73.3个月 ) ,结果为良性组 4例均无瘤生存 ;交界性组 2例无瘤生存 ,1例失访 ,1例在无瘤生存 18个月后失访 ;恶性组无瘤生存有 7例 ,带瘤生存者及死亡有 16例 ,2例失访。Ki6 7染色阴性 2例 ,均为良性 ;其余 31例阳性中 ,增殖指数 (阳性细胞数 )大于 5 %的有恶性 16例 (94 .1% ) ,交界性 1例。在免疫组化p5 3染色中 ,恶性组 2 5例均呈阳性表达 (平均阳性细胞数 4 2 .9% ) ,其中 12例阳性细胞数大于 5 0 %。当Ki6 7和 p5 3均为阴性或两者阳性细胞数分别是 <1%和 <10 %时 ,肿瘤呈良性经过 ,预后     

11β-羟类固醇脱氢酶与2型糖尿病

11β-羟类固醇脱氢酶(11β-HSDs)催化氢化可的松(皮质酮)和可的松(脱氢皮质酮)的相互转化，对调节局部糖皮质激素的活性起着重要作用，而糖皮质激素可诱导胰岛素抵抗。肝、脂肪等组织内11β-HSD活性的改变与肥胖、2型糖尿病等疾病密切相关。     

Studies on monoclonal anti-isotypic and anti-idiotypic antibodies against leukemia and myeloma: IV modulation of membrane fluidity of leukemic cell lines and tonsillar cell stimulated with McAbs

Summary In this study the technique of labelling the cell membrane with DPH fluorescence polarization was used to observe the membrane fluidity of B lymphocytic cell lines and tonsillar cells from healthy persons; the modulation effect on membrane-fluidity induced by McAbs against isotypic and idiotypic determinants of IgM from patients with leukemia was studied as well. The expression of the corresponding isotypic and idiotypic determinants of IgM on the cell membrane was determined. The results show that the membrane fluidity of leukemic cell lines is remarkably higher than that of tonsillar cells from healthy persons, and McAbs against isotypic determinants of leukemic IgM can enhance the membrane fluidity of all kinds of cells mentioned above. However, the anti-idiotypic monoclonal antibody increased only the membrane fluidity of leukemic cell lines. These results indicated that there was a close relationship between the effect of McAbs on cell membrane fluidity and the expression of corresponding isotypic and idiotypic determinants of IgM on the cell membrane.     

浅论记者的职业心理动机

采访与写作是新闻记者的一种社会性活动，社会的政治经济动向、科教文化潮流、外交军事突变以及各种风云人物的活动都是这一工作反映的内容。要写出一篇好的作品．必须要有一定的社交能力、应变能力、观察能力及写作能力．这是脑力劳动和体力劳动的综合行为。当记者在枯燥的材料堆里翻开资料，当他与一个初次采访的人物去谈话．在面临危险的环境中找新闻时……．是何种动机支配着记者去克服困难．完成这些采访任务的呢？心理学认为动机是直接推动个体活动以达到一定目的的内部动力。     

Physical and spectroscopic methods for the evaluation of the interactions of antimitotic agents with tubulin.

The physico-chemical methods for the study of the binding of ligands to tubulin are examined in-depth, emphasizing the assumptions on which they are based and their limitations. The criteria of specificity and linkage to protein self-association are presented. It is shown that, of the direct equilibrium binding techniques, Hummel-Dreyer gel permeation chromatography and rapid ultracentrifugation are applicable only when binding is not linked to protein self-association. Disc filtration is valid only when the reverse unbinding reaction is very low. Binding linked to protein self-association can be measured by batch gel permeation or by dialysis equilibrium. The indirect techniques, such as fluorescence perturbation or difference absorption spectroscopy are discussed in terms of the assumptions on which they are based. They are shown to be used best only after characterization of the binding by direct techniques. Equilibrium binding parameters can also be deduced from careful kinetic experiments. Comparison of calorimetrically measured enthalpies of binding to van't Hoff enthalpies derived from equilibrium measurements indicates that the method of choice is calorimetric, while comparison with van't Hoff analysis can reveal the existence of reaction steps not detected by equilibrium measurements. Use of other indirect approaches, such as titration of an enzymic activity, can also lead to the detection of additional steps. The criteria are set up for the proper data analysis of ligand binding linked to protein self-association and the selection of the proper mode of linkage. It is shown how the thermodynamic contributions of various moieties of a ligand can be established by a rational structural modification of the ligand and the proper analysis of the binding measurements, in which all non-specific entropic contributions are taken into account. It is demonstrated also how a similar analysis of binding data can lead to conclusions about the reaction pathway from a comparison of equilibrium thermodynamic measurements on judiciously modified ligand molecules.     

支气管扩张症1例

患儿,男,12岁,以反复咳嗽、咯痰5年、加重2个月为主诉入院。5年前开始反复咳嗽,咯黄痰,每年至少发作4次,每次持续10~20 d。近2个月病情明显加重,无咯血,盗汗较重,体质量无减轻,无结核接触史。查体:神清,呼吸平稳,营养中等,咽充血,扁桃体Ⅰ度大。双肺底可闻及密集吸气末中小湿音,以左肺底为主。血白细胞17.4×109/L,中性粒细胞0.84,红细胞5.0×1012/L,血红蛋白151 g/L,血沉53 mm/1 h,降钙素原1.56 ng/L,C-反应蛋白67.4 mg/L。病原学检查:肺炎支原体、肺炎衣原体、腺病毒、合胞病毒、流感病毒、副流感病毒、柯萨奇病毒、埃可病毒、腮腺炎病…