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21.
Young June Choe Eun Hwa Choi Jong Woon Choi Byung Wook Eun Lucy Youngmin Eun Yae-Jean Kim Yeo Hyang Kim Young A. Kim Yun-Kyung Kim Ji Hee Kwak Hyuk Min Lee Hyunju Lee Joon Kee Lee June Dong Park Eun-Jin Kim Young Joon Park Jin Gwack Sang Won Lee 《Emerging infectious diseases》2021,27(4):1196
A concerning development during the coronavirus disease pandemic has been multisystem inflammatory syndrome in children. Reports of this condition in East Asia have been limited. In South Korea, 3 cases were reported to the national surveillance system for multisystem inflammatory syndrome in children. All case-patients were hospitalized and survived with no major disease sequelae. 相似文献
22.
Chae HJ Kim HR Lee WG Kwak YK Kim WH Hong ST Cho GH Kim JS Chae SW 《Immunopharmacology and immunotoxicology》2005,27(2):211-232
Combined radiotherapy and chemotherapy have represented major advance in the therapeutic management of cancer therapy. Anthracycline antineoplastic agents are limited by a high incidence of severe and usually irreversible cardiac toxicity, the cause of which remains controversial. When the primary cardiomyocytes isolated from neonatal rats were preirradiated by γ-ray, the cells were highly resistant to adriamycin-induced apoptosis. This study shows that irradiation inhibited apoptosis by enhancing Bcl-2, attenuating Bax induction, and preventing collapse of mitochondrial membrane potential (ΔΨ), cytochrome c release into cytoplasm and caspase-3, -6 and -9 activations.
In addition, the preirradiation stimulated the activity of manganese-superoxide dismutase (Mn-SOD) and the expression of Mn-SOD mRNA and protein. Adriamycin decreased Mn-SOD activity but did not change the activity of copper/zinc (Cu/Zn)-SOD under either pre- or nonirradiated condition. Phosphothioate-linked antisense against Mn-SOD, which specifically knocked down the activity of Mn-SOD but not that of Cu/Zn-SOD, reversed irradiation-induced protective effect in adriamycin-exposed cardiomyocytes. These data suggest that the irradiation-induced expression of Mn-SOD plays an important role in irradiation-mediated protection in adriamycin-exposed rat ventricular cardiomyocytes. 相似文献
In addition, the preirradiation stimulated the activity of manganese-superoxide dismutase (Mn-SOD) and the expression of Mn-SOD mRNA and protein. Adriamycin decreased Mn-SOD activity but did not change the activity of copper/zinc (Cu/Zn)-SOD under either pre- or nonirradiated condition. Phosphothioate-linked antisense against Mn-SOD, which specifically knocked down the activity of Mn-SOD but not that of Cu/Zn-SOD, reversed irradiation-induced protective effect in adriamycin-exposed cardiomyocytes. These data suggest that the irradiation-induced expression of Mn-SOD plays an important role in irradiation-mediated protection in adriamycin-exposed rat ventricular cardiomyocytes. 相似文献
23.
应用四唑盐比色法对5种骨水泥的细胞毒性的测试 总被引:4,自引:1,他引:3
0 引言 应用细胞培养法对牙科材料的生物相容性进行评价是一种简便、有效、经济的方法 .近年来 ,国内外有许多学者报道用 MTT检测细胞活性 ,并认为用 MTT法可以代替活细胞计数、同位素标记进行细胞毒性试验 .我们应用 MTT法对 5种骨水泥的细胞毒性进行了评价 ,并对有关问题进行了讨论 .1 材料和方法1.1 测试材料 1号 :生物水泥 ,2号 :羟基磷灰石人工骨 ,3号 :氰基丙烯酸酯骨水泥 1号 (自制 ) ,4号 :氰基丙烯酸酯骨水泥 2号 (自制 ) ,5号 :氰基丙烯酸酯骨水泥 3号 (自制 ) .阴性对照采用珊瑚 ,阳性对照采用聚氯乙烯 .1.2 实验步骤… 相似文献
24.
目的合成三尖杉碱吲哚类似物。方法以色胺为原料经缩合、环合、水解与脱羧反应得吲哚哌啶酮化合物,经Bischler-Napieralski反应得烯胺。通过烯胺的溴化与氧化-重排反应得到吲哚氮杂 相似文献
25.
Hwan Bae Lee Jong Hwan Kwak Ok Pyo Zee Seung Jo Yoo 《Archives of pharmacal research》1994,17(4):273-277
Six flavonoids were isolated from the aerial parts ofCirsium rhinoceros. The flavonoids were identified as apigenin, luteolin, pectolinarigenin-7-O-β-D-glucopyranoside, linarin, pectolinarin and hispidulin-7-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside on the basis of chemical and spectral evidence. 相似文献
26.
Kwak JY Beer AE Kim SH Mantouvalos HP 《American journal of reproductive immunology (New York, N.Y. : 1989)》1999,41(1):91-98
PROBLEM: Placental lesions of 71 women with documented recurrent spontaneous abortions of unknown etiology were evaluated using immunohistochemical staining. METHOD OF STUDY: Placental tissue blocks (less than 12 weeks gestation) from prior pregnancy losses were obtained, recut, and analyzed utilizing monoclonal antibody to identify the trophoblast (cytokeratin 8/18) and natural killer (NK) cells (CD57) at the implantation site. The following features were evaluated: trophoblast invasion pattern; syncytium formation; vasculitis and thromboembolism of decidual vessels; decidual inflammation; decidual necrosis; fibrin deposition at the decidual necrosis site; mononuclear-cell infiltration in villi and intervillous space; perivillous fibrin deposition; trophoblast morphology; and quantitation of CD57+ NK cells within the decidual tissue near the implantation site. Controls consisted of 20 healthy women with no history of recurrent pregnancy losses, who had their pregnancies electively terminated. RESULTS: Of the women studied, 29.6% demonstrated elevated CD57+ NK cells at the implantation site (P = 0.030), 54.1% had inadequate cytotrophoblast invasion depth (P = 0.000), 44.1% demonstrated inadequate syncytium formation (P = 0.004), and 33.9% presented thromboembolism in decidual vessels (P = 0.025). CONCLUSION: Some women with recurrent spontaneous abortions demonstrate abnormal placental lesions at the implantation site. Immunopathologic evaluation of the placental implantation site that terminated in a spontaneous abortion may reveal the immunopathogenesis of previous pregnancy losses. 相似文献
27.
28.
Cytotoxicity of urushiols isolated from sap of Korean lacquer tree (Rhus vernicifera stokes) 总被引:1,自引:0,他引:1
Hong DH Han SB Lee CW Park SH Jeon YJ Kim MJ Kwak SS Kim HM 《Archives of pharmacal research》1999,22(6):638-641
Cytotoxicities of four urushiols, congeners isolated from the sap of Korean lacquer tree (Rhus vernicifera Stokes), to 29 human cancer cell lines originated from 9 organs were evaluated. Their values of 50% growth inhibition were below 4 microg/ml, and showed cell line specific cytotoxicity. The present result is the first report on the cytotoxicity of urushiols suggesting that they would have an anticancer activity to human cancer cells. 相似文献
29.
Matthias Grube Katayoun Rezvani Adrian Wiestner Hiroshi Fujiwara Giuseppe Sconocchia Jan J Melenhorst Nancy Hensel Gerald E Marti Larry W Kwak Wyndham Wilson John A Barrett 《Clinical cancer research》2004,10(3):1047-1056
PURPOSE: To investigate potential immunotherapeutic strategies in B lymphocytic malignancies we looked for CTLs recognizing CD19 and CD20 epitopes. EXPERIMENTAL DESIGN: Three CD19 and CD20 peptides binding to HLA-A*0201 were identified and used to detect peptide specific CTLs by a quantitative real-time PCR to measure IFN-gamma mRNA expression in 23 healthy individuals and 28 patients (18 chronic lymphocytic leukemia (CLL), 7 follicular lymphoma, 2 acute lymphocytic leukemia, and 1 large cell lymphoma). Peptide-specific CTLs were expanded in culture with CD40-activated B cells to test lytic activity in three patients. RESULTS: In healthy individuals, CD8+ T-cell responses were detected in one to CD19(74-82), in three to CD20(127-135), and three to CD20(188-196). Seven of 27 patients (6 with CLL) had CD8+ T cells recognizing CD19(74-82). Seven patients responded to CD20(127-135) and three to CD20(188-196). All were CLL patients. CD19(74-82)-specific CTLs from three patients were expanded over 4 weeks. These cells were HLA-A*0201 specific and lytic for peptide-loaded antigen-presenting cells but not to malignant or unpulsed B cells. CONCLUSIONS: CTLs that recognize CD19 and CD20 epitopes exist in healthy individuals and may be increased in CLL patients. They are of low avidity and require high doses of peptide for activation. Strategies to increase T-cell avidity would be necessary for T-cell immunotherapeutic approaches using the peptides studied. 相似文献
30.
Sattva S Neelapu Sivasubramanian Baskar Barry L Gause Carol B Kobrin Thelma M Watson Andrea Robin Frye Robin Pennington Linda Harvey Elaine S Jaffe Richard J Robb Mircea C Popescu Larry W Kwak 《Clinical cancer research》2004,10(24):8309-8317
PURPOSE: The idiotype (Id) of the immunoglobulin on a given B-cell malignancy is a clonal marker that can serve as a tumor-specific antigen. We developed a novel vaccine formulation by incorporating Id protein with liposomal lymphokine that was more potent than a prototype, carrier-conjugated Id protein vaccine in preclinical studies. In the present study, we evaluated the safety and immunogenicity of this vaccine in follicular lymphoma patients. EXPERIMENTAL DESIGN: Ten patients with advanced-stage follicular lymphoma were treated with five doses of this second generation vaccine after chemotherapy-induced clinical remission. All patients were evaluated for cellular and humoral immune responses. RESULTS: Autologous tumor and Id-specific type I cytokine responses were induced by vaccination in 10 and 9 patients, respectively. Antitumor immune responses were mediated by both CD4+ and CD8+ T cells, were human lymphocyte antigen class I and II associated, and persisted 18 months beyond the completion of vaccination. Specific anti-Id antibody responses were detected in four patients. After a median follow-up of 50 months, 6 of the 10 patients remain in continuous first complete remission. CONCLUSIONS: This first clinical report of a liposomal cancer vaccine demonstrates that liposomal delivery is safe, induces sustained tumor-specific CD4+ and CD8+ T-cell responses in lymphoma patients, and may serve as a model for vaccine development against other human cancers and infectious pathogens. 相似文献