Astragaloside IV inhibits palmitate-mediated oxidative stress and fibrosis in human glomerular mesangial cells via downregulation of CD36 expression

Background

The increased in?ux of free fatty acids (FFAs) into the kidney is a risk factor for diabetes nephropathy (DN). In the present study we investigated the effects of astragaloside IV (AS-IV) on FFA-induced lipid accumulation, oxidative stress, and activation of TGF-β1 signaling in human glomerular mesangial cells (HMCs).

既往血吸虫感染者体检数据分析及其与脂肪肝、冠心病的关系

目的 了解既往血吸虫感染者的健康状况,并研究其与脂肪肝、冠心病的关系。方法 在华东疗养院2016年6万余例体检人群中,筛选出既往血吸虫感染者272人为研究对象;采用计算机产生的随机数字以1∶100的比例,从同期体检人群中抽取277人为对照组。收集研究对象的详细病史、彩色多普勒超声和实验室检查结果。利用Logistic回归分析既往血吸虫感染与脂肪肝、冠心病等疾病的关系。结果 既往血吸虫感染组中脂肪肝和冠心病患病人数分别为26例和5例,对照组相应为119例和14例,两组比较差异均有统计学意义（P<0.05）;既往血吸虫感染组的TG、AIP、空腹血糖以及血小板分别为（1.28±0.67） mmol/L、（-0.08±0.27）、（5.82±1.19） mmol/L和（182.01±49.20）×10⁹/L,均比对照组的（1.79±1.26） mmol/L、（0.11±0.30）、（6.09±1.63） mmol/L和（199.22±47.03）×10⁹/L低,两组比较差异均有统计学意义（P<0.05）;既往血吸虫感染组的HDL-C为（1.42±0.35） mmol/L¹、总胆红素为（14.59±5.08） μmol/L,分别与对照组的（1.22±0.34） mmol/L和（13.26±4.38） μmol/L比较差异均有统计学意义（P<0.05）。结论 既往血吸虫感染者高血压、糖尿病患病率与同年龄段人群相仿,但既往血吸虫感染可能与轻度脂肪肝、冠心病呈负相关。     

化疗后血红蛋白水平与晚期胃肠癌患者临床疗效的相关性分析

目的：分析化疗后血红蛋白（Hb）水平与晚期胃肠癌患者临床疗效的相关性。方法收集我院2006年6月-2012年6月符合条件的晚期胃肠癌患者80例，对其化疗前后的临床资料包括Hb水平、生存情况和临床疗效等进行回顾性分析。结果化疗前男女性患者间以及男性患者化疗前后的Hb值差异有统计学意义（P〈0.05）。PR和SD患者化疗前后Hb值之间的差异均无统计学意义（P〉0.05）；PD患者化疗前后Hb值之间的差异具有统计学意义（P〈0.05）。贫血程度较轻的患者平均生存时间相对长，但差异并无统计学意义。结论晚期胃肠癌患者化疗后的Hb值的变化与化疗疗效相关，化疗前后Hb值与生存时间及生存率呈正相关，临床应重视化疗所致的贫血的治疗。     

大剂量甲氨蝶呤治疗儿童急性淋巴细胞白血病79例毒副作用临床观察

目的观察大剂量甲氨蝶呤(HD-MTX)治疗儿童急性淋巴细胞白血病(ALL)所致毒副作用的临床特点。方法 79例ALL患儿按诊断标准分为标危组(MTX使用剂量为3g/m~2)和中高危组(MTX使用剂量为5g/m~2),接受HD-MTX治疗。在MTX开始36h后予亚叶酸钙解救,检测42~48h MTX血药浓度,同时观察患儿HD-MTX治疗后的毒副作用。结果 79例ALL患儿接受HD-MTX化疗后,发生中性粒细胞减少、血红蛋白降低和血小板减少的分别为52例(66%)、43例(54%)和10例(13%),发生肝脏毒性、黏膜损害和胃肠道反应者各为24例(30%)、29例(37%)和16例(20%),无一例发生肾脏毒性。42~48h MTX血药浓度与毒副作用的发生风险差异有显著性(P0.05)。比较标危组与中高危组MTX使用毒副作用的发生风险差异无显著性(P0.05)。结论 HD-MTX治疗ALL时毒副作用多为骨髓抑制、胃肠道反应、黏膜损害和肝脏毒性。毒副作用的发生与MTX血药浓度有关联,未发现使用3g/m~2和5g/m~2的MTX剂量与毒副作用的发生风险存在关联。     

Molecular identification of twelve medicinal plants of Huperziaceae

Twelve species of Huperziaceae have been identified for resolving the resource depletion of Huperziaceae plants. The chloroplast genes rbcL, rpL16, and psbA-trnH intergenic spacers were used in this study. Identification of different species of Huperziaceae is possible by analyzing the information obtained from rbcL, rpL16, and psbA-trnH intergenic spacer sequences. The result also can provide information with regard to the use of plant tissue culture and cuttage propagation to solve the problem of the resource depletion of these plants.     

Simultaneous knockdown of APRIL via multiple shRNAs reduces the malignancy of SW480 cells

A proliferation-inducing ligand (APRIL) is a key factor involved in the tumor development and progression in some tumor tissues and cells. Its overexpression and as gene target in SW480 colon carcinoma cells was confirmed in our previous study. To seek a more potent way to treat colon carcinoma using a gene therapy method, herein, we constructed a multiple short hairpin RNA (shRNA) expression vector containing four shRNAs against the APRIL gene in SW480 cells. APRIL expression levels and cell biological behavior were detected after transfection with different kinds of vectors. As expected, we found that our multiple shRNA vector produced a more significant knockdown effect of APRIL than the vectors containing only one APRIL shRNA. Furthermore, our findings indicate that silencing APRIL expression in SW480 cells decreased their malignancy by reducing proliferation, invasion and adhesion, as well as inducing apoptosis. Based on our findings, vectors containing multiple shRNAs to silence the expression of APRIL may be exploited as a novel therapeutic strategy for tumors.     

高通量药物敏感性筛选系统在非小细胞肺癌伴恶性胸腔积液治疗中的应用

目的 ·探讨体外高通量药物敏感性筛选系统指导非小细胞肺癌(non-small cell lung cancer,NSCLC)伴恶性胸腔积液(malignant pleural effusion,MPE)患者个体化治疗的可行性.方法 ·纳入2019年1月—2020年5月就诊于南京市胸科医院的30例NSCLC伴MPE患者,...     

Single nucleotide polymorphisms of matrix metallopeptidase 3 and risk of gliomas in a Chinese han population

Matrix metallopeptidases (MMPs) play an important role in central nervous system tumor growth, invasion and spreading. The currently available data provide clear evidence for the involvement of MMP3 in the pathophysiology of glioma. The study aims to explore the association of single nucleotide polymorphisms (SNPs) across the MMP3 gene with glioma risk. Three haplotype tagging and additional two promoter SNPs were genotyped among 766 glioma patients and 824 cancer-free controls from East China. None of these polymorphisms alone had a significant effect on risk of gliomas. However, when three promoter polymorphisms were evaluated together by the number of putative risk of genotypes (i.e., rs645419AA, 632478CA+AA, rs522616AA), a statistically significantly increased risk of gliomas was associated with the combined genotypes with two to three risk genotypes, compared with those with zero to one risk genotypes (adjusted odds ratio (OR)?=?1.32; 95% confidence interval (CI)?=?1.03-1.68). This increased risk was also more pronounced among adults (adjusted OR?=?1.14, 95%CI?=?1.02-1.27), males (adjusted OR?=?1.19, 95%CI?=?1.05-1.36), smokers (adjusted OR?=?1.28, 95%CI?=?1.07-1.52), subjects with no family history of cancer (adjusted OR?=?1.21, 95%CI?=?1.07-1.37), and patients with nonastrocytic gliomas (adjusted OR?=?1.23, 95%CI?=?1.06-1.43). In summary, our results suggest that any one of MMP3 variants may not have a substantial effect on glioma risk, but a joint effect of MMP3 promoter polymorphisms may contribute to risk of gliomas, particularly for adult gliomas. ? 2011 Wiley Periodicals, Inc.     

酵母双杂交系统在膜蛋白相互作用研究中的应用

膜相关蛋白约占细胞总蛋白质中的1/3,它们大都参与了细胞的诸多生理、病理过程和药物反应机理.研究膜蛋白的相互作用对于揭示细胞的生命活动规律及寻找药物作用靶标都有重要的意义.由于膜蛋白本身的特性及其难以进入核内等原因,经典的酵母双杂交技术并不适用于检测膜蛋白间的相互作用.针对在活细胞中研究膜蛋白相互作用的需要,近年来国际上先后发展了一系列用于膜蛋白相互作用研究的酵母双杂交新系统,并取得了许多重要发现.