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41.
Doi A Okano M Akagi H Nishizaki K Taguchi T Murakami T Ohtsuka A 《Anatomical science international / Japanese Association of Anatomists》2003,78(1):62-67
The musk shrew, Suncus murinus, is one of the primitive mammals and has a pair of palatine tonsils. In the present study,
we investigated the blood microvascular architecture of the tonsil in this animal by scanning electron microscopy of corrosion
casts. The paranodular arterioles entered the lymph nodule to form a coarse capillary plexus within the nodule. Some of the
arterioles reached the dome region to give rise to a fine meshwork of dome subepithelial capillaries. This dome subepithelial
capillary network did not show any hairpin or switch-back patterns, as seen in human and rabbit tonsils. Both of the nodular
and dome capillaries were drained into the postcapillary venules in the periphery of the nodular or the paranodular region.
On the surface of these cast venules, oval-shaped indentations were seen corresponding to the luminal surface of the high
endothelial venules. These venules were collected into the large vein at the bottom of the tonsil. The blood vascular architecture
of the musk shrew tonsil is basically the same as those of other mucosa-associated lymphoid tissues in mammals. 相似文献
42.
Masayuki Ishihara PhD Kiyohaya Obara MD Singo Nakamura PhD Masanori Fujita MD PhD Kazunori Masuoka MD Yasuhiro Kanatani MD PhD Bonpei Takase MD PhD Hidemi Hattori PhD Yuji Morimoto MD PhD Miya Ishihara PhD Tadaaki Maehara MD PhD Makoto Kikuchi PhD 《Journal of artificial organs》2006,9(1):8-16
An aqueous solution of photocrosslinkable chitosan containing azide groups and lactose moieties (Az-CH-LA) incorporating paclitaxel
formed an insoluble hydrogel within 30 s of ultraviolet light (UV) irradiation. The chitosan hydrogel showed strong potential
for use as a new tissue adhesive in surgical applications and wound dressing. The fibroblast growth factor (FGF)-2 molecules
retained in the chitosan hydrogel and in an injectable chitosan/IO4-heparin hydrogel remain biologically active, and were gradually released from the hydrogels as they biodegraded in vivo.
The controlled release of biologically active FGF-2 molecules from the hydrogels caused induction of angiogenesis and collateral
circulation occurred in healing-impaired diabetic (db/db) mice and in the ischemic limbs of rats. Paclitaxel, which is an antitumor reagent, was also retained in the chitosan hydrogel
and remained biologically active as it was released on degradation of the hydrogel in vivo. The chitosan hydrogels incorporating
paclitaxel effectively inhibited tumor growth and angiogenesis in mice. The purpose of this review is to describe the effectiveness
of chitosan hydrogel as a local drug delivery carrier for agents (e.g., FGF-2 and paclitaxel) to control angiogenesis. It
is thus proposed that chitosan hydrogel may be a promising new local carrier for drugs such as FGF-2 and paclitaxel to control
vascularization. 相似文献
43.
Watanabe M Goto N Watanabe Y Nishiguchi S Shimada K Yasunga T Yamanishi H 《International journal of molecular medicine》2005,15(4):561-566
Interleukin-18 (IL-18) is one of the pivotal cytokines controlling the defense mechanism called inflammation. As a first step to develop proteins for controlling the IL-18 level, we initiated a study of IL-18-binding proteins (IL-18BPs). Twenty-four IL-18BP family members, 11 from vertebrates and 13 from chordopoxviruses, were picked from the NCBI database. Eight of these vertebrate IL-18BPs and two of the chordopoxvirus IL18-BPs were identified here and characterized as new members of the IL-18BP family. Their IL-18 binding domains were aligned and the distribution of highly conserved critical amino acid residues was analyzed and used to construct a phylogenetic tree. From this tree it was inferred that at least two independent events created two different ancestral viral IL-18BP genes by retroposition of IL-18BP genes from the vertebrate lineage. These two events are estimated to have occurred after an ancient mammalian IL-18BP gene diverged from birds, and before the mammalian IL-18BP gene diverged into human, ungulate and rodent IL-18BP genes. Moreover, our results suggest that IL-18BP and interleukin-1 receptor, type II (IL-1R2) had a common ancestral gene and diverged from the ancestral gene into IL-18BP and IL-1R2 genes in the fish period. 相似文献
44.
Hajime Kuhara Kazunori Ikei Takashi Wakabayashi Hidemasa Kishimoto 《Pathology international》1986,36(7):1083-1088
A 58-year-old man with liposarcoma originating from the right paratesticular area was reported. Histologically, liposarcoma showed a well-differentiated sclerosing type. Coexistence of transitional cell carcinoma of the urinary bladder and liposarcoma was also revealed. The cases of paratesticular liposarcoma in the literature are briefly reviewed. 相似文献
45.
Masahito Katoh Yasushi Itoh Kazumasa Ogasawara Kiichi Kajino Hiroki Nishihori Akio Takahashi Naoto Matsuki Kazuya Iwabuchi Takato O. Yoshida Robert A. Good Kazunori Ono 《European journal of immunology》1996,26(5):976-980
In a previous study, we identified T cell receptor and major histocompatibility complex (MHC) contact sites on the pigeon cytochrome c p43-58 peptide. Positions 46 and 54 of p43-58 were shown to be the MHC-binding sites. Specific amino acids were identified on the MHC-binding sites which bound to the relevant I-A molecule. In the present study, using NOD (I-Ag7) mice, we established a T cell hybridoma, NOE33-1-2, specific for a p43-58 analog 46R50E54A with arginine (R) and alanine (A) at positions 46 and 54, respectively. Interestingly, NOE 33-1-2 recognized 46R50E54A in the presence of not only I-Ag7, but also I-Ad, s, u and v. In contrast to previous reports that promiscuous T cells were able to recognize peptide antigens with various HLA-DR or I-E molecules consist of monomorphic α and polymorphic β chains, the promiscuous T cell clone NOE33-1-2 recognized peptides with various I-A molecules lacking the monomorphic chain. 相似文献
46.
A common mutation in methylenetetrahydrofolate reductase gene among the Japanese population 总被引:7,自引:0,他引:7
Hisahide Nishio Myeong Jin Lee Motoko Fujii Kazuomi Kario Kazunori Kayaba Kazuyuki Shimada Masafumi Matsuo Kimiaki Sumino 《Journal of human genetics》1996,41(2):247-251
Summary Hyperhomocysteinemia has been reported as an independent risk factor for atherosclerotic cerebrovascular and coronary heart diseases. 5,10-Methylenetetrahydrofolate reductase (MTHFR) is one of the enzymes responsible for hyperhomocysteinemia. The C to T transition of the MTHFR gene at nucleotide position 677 results in decreasing the enzymatic activity and increasing the plasma homocysteine level. We studied the distribution of the MTHFR gene mutation among the Japanese population. The subjects were 129 Japanese males (aged 40–59 years). The allele frequency of the mutation was 0.38. The frequencies of the three genotypes were as follows: +/+, 11%; +/–, 54%; –/–, 35% (+ and – indicate the presence and absence of the mutation, respectively). We also studied the frequency of the MTHFR gene mutation in the middle-aged Japanese males with hypertension to investigate the possibility that this mutation is related to essential hypertension. The normotensive and hypertensive subjects were identical in the distribution of the mutated allele and the frequencies of the three genotypes. Furthermore, the prevalence of hypertension in each genotype group was same, although the mean diastolic pressure of the group with homozygous mutation was significantly higher than that of other groups (p<0.05). 相似文献
47.
Fumiyuki Yamasaki Yoshinori Kajiwara Seiji Hama Taro Murakami Toshikazu Hidaka Taiichi Saito Hiroyuki Yoshioka Kazuhiko Sugiyama Kazunori Arita Kaoru Kurisu 《Pathobiology》2007,74(1):22-31
OBJECTIVE: To investigate the mechanism of staurosporine-induced glioma cell death and cell cycle arrest using adenovirus-mediated gene transfection, as well as the function of retinoblastoma (Rb) and genetic instability induced by staurosporine. METHODS: Cell cycle regulation, cell death and nuclear abnormalities induced by staurosporine were examined using an adenovirus vector expressing Rb, p16 or p21 genes in human glioma cell lines. RESULTS: The Rb-defective SF-539 cell line was resistant to staurosporine compared with cell lines expressing intact Rb. SF-539 glioma cells exposed to staurosporine became multinucleated and then died. Multinucleation was prevented in SF-539 cells transfected with the Rb gene, thus decreasing the death rate of these cells. CONCLUSIONS: These results imply that enforced Rb expression protects cells from genomic instability induced by staurosporine regardless of its upstream molecular effects. 相似文献
48.
49.
Masayuki Yokoyama Hayato Anazawa Akeo Takahashi Shohei Inoue Kazunori Kataoka Nobuhiko Yui Yasuhisa Sakurai 《Macromolecular chemistry and physics.》1990,191(2):301-311
Novel segmented multiblock copolymers ( 7 ) were synthesized by linking poly(ethylene oxide) (PEO) blocks with poly(β-benzyl L -aspartate)(PBLA) blocks via urethane and urea bonds, which were formed by the reaction of 4,4′-methylenediphenyl isocyanate ( 5 ) with the terminal hydroxyl groups of α-hydro-ω-hydroxypoly(oxyethylene) ( 4 ) and the terminal amino groups of poly(β-benzyl L -aspartate)-block-iminohexamethyleneimino-block-poly(β-benzyl L -aspartate) ( 3 ) [prepared from 1,6-hexanediamine ( 1 ) and β-benzyl L -aspartate N-carboxy anhydride ( 2 )], respectively. Membranes with various water contents were obtained from these copolymers by changing the lengths of the PEO and PBLA segments. The study of the permeation of 1-phenyl-1,2-ethanediol, vitamin B12 and myoglobin through the membranes showed a high dependency of the permeability on the molecular weight of the solutes. 相似文献
50.
Taki Y Goto R Evans A Zijdenbos A Neelin P Lerch J Sato K Ono S Kinomura S Nakagawa M Sugiura M Watanabe J Kawashima R Fukuda H 《Neurobiology of aging》2004,25(4):455-463
The objectives of this study were to evaluate the correlations of the volumes of the gray matter and white matter with age, and the correlations of the tissue probabilities of the gray matter and white matter with age and several cerebrovascular risk factors. We obtained magnetic resonance (MR) images of the brain and clinical information from 769 normal Japanese subjects. We processed the MR images automatically by correcting for inter-individual differences in brain size and shape, and by segmenting the MR images into the gray matter and white matter. Volumetry of the brain revealed a significant negative correlation between the gray matter volume and age, which was not observed between white matter volume and age. Voxel-based morphometry showed that age, systolic blood pressure, and alcohol drinking correlated with the regional tissue probabilities of the gray matter and white matter. 相似文献