全文获取类型
收费全文 | 52101篇 |
免费 | 4194篇 |
国内免费 | 118篇 |
专业分类
耳鼻咽喉 | 637篇 |
儿科学 | 1846篇 |
妇产科学 | 1216篇 |
基础医学 | 6578篇 |
口腔科学 | 717篇 |
临床医学 | 8089篇 |
内科学 | 9409篇 |
皮肤病学 | 620篇 |
神经病学 | 5060篇 |
特种医学 | 1197篇 |
外国民族医学 | 2篇 |
外科学 | 5384篇 |
综合类 | 669篇 |
一般理论 | 58篇 |
预防医学 | 6935篇 |
眼科学 | 625篇 |
药学 | 3634篇 |
中国医学 | 65篇 |
肿瘤学 | 3672篇 |
出版年
2023年 | 387篇 |
2022年 | 542篇 |
2021年 | 1069篇 |
2020年 | 741篇 |
2019年 | 1153篇 |
2018年 | 1368篇 |
2017年 | 980篇 |
2016年 | 1053篇 |
2015年 | 1152篇 |
2014年 | 1624篇 |
2013年 | 2464篇 |
2012年 | 3635篇 |
2011年 | 3828篇 |
2010年 | 2039篇 |
2009年 | 1882篇 |
2008年 | 3371篇 |
2007年 | 3385篇 |
2006年 | 3298篇 |
2005年 | 3237篇 |
2004年 | 2994篇 |
2003年 | 2692篇 |
2002年 | 2603篇 |
2001年 | 725篇 |
2000年 | 635篇 |
1999年 | 659篇 |
1998年 | 598篇 |
1997年 | 499篇 |
1996年 | 413篇 |
1995年 | 366篇 |
1994年 | 355篇 |
1993年 | 340篇 |
1992年 | 521篇 |
1991年 | 446篇 |
1990年 | 389篇 |
1989年 | 386篇 |
1988年 | 413篇 |
1987年 | 361篇 |
1986年 | 337篇 |
1985年 | 291篇 |
1984年 | 340篇 |
1983年 | 261篇 |
1982年 | 222篇 |
1981年 | 205篇 |
1980年 | 176篇 |
1979年 | 229篇 |
1978年 | 150篇 |
1977年 | 140篇 |
1976年 | 146篇 |
1975年 | 146篇 |
1974年 | 135篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
201.
Taylor LE Rich JD Tashima KT 《The New England journal of medicine》2004,351(22):2340-2; author reply 2340-2
202.
Zhao H Bailey LA Elsas LJ Grinzaid KA Grabowski GA 《American journal of medical genetics. Part A》2003,(1):52-56
Gaucher disease, a common lysosomal storage disorder, is associated with mutations at the acid beta-glucosidase (GCase) locus. Two affected individuals are described to share a common mutant allele, but manifest different clinical categorical phenotypes. A 57-year-old female, with Gaucher disease type 1 and Cherokee ancestry, was homozygous for a rare mutant allele encoding Lys79Asn (K79N). A 2-year-old Caucasian male, with Gaucher disease type 3 and Cherokee ancestry, was a heteroallelic homozygote for this same allele (K79N) and a novel complex mutation (null allele). The shared alleles were identical as determined by complete gene sequencing, suggesting a founder effect. The discrepant phenotypes (types 1 and 3) in these two patients provide support for a threshold of residual activity necessary to "protect" the central nervous system (CNS) from the pathogenic effects of Gaucher disease, indicating an allele dose-effect. Designation of genotype associations with specific phenotypes must be assessed with this perspective. 相似文献
203.
A new case of congenital dyserythropoietic anaemia, type III: studies of the cell cycle distribution and ultrastructure of erythroblasts and of nucleic acid synthesis in marrow cells. 下载免费PDF全文
S N Wickramasinghe T E Parry C Williams A N Bond M Hughes S Crook 《Journal of clinical pathology》1982,35(10):1103-1109
The clinical and laboratory findings in an asymptomatic 19-year-old Welshman with congenital dyserythropoietic anaemia (CDA) type III are described. The blood film showed macrocytosis and red cell fragmentation and there was biochemical evidence of intravascular haemolysis. The bone marrow showed erythroid hyperplasia, megaloblastic erythropoiesis and several giant multinucleate erythroblasts. Some mononucleate erythroblasts were large and had relative DNA contents of 4-8c and the bi- and multinucleate erythroblasts had total DNA contents of 2-16c. Some of the multinucleate erythroblasts displayed a variety of ultrastructural abnormalities, including marked differences in the appearances of the individual nuclei within the same cell. The marrow cells gave a normal deoxyuridine-suppressed value indicating that the megaloblastic changes were not caused by an impairment of the methylation of deoxyuridylate. The rates of incorporation of 14C-glycine and 14C-adenine into both the DNA and RNA of bone marrow cells were within the normal range. Furthermore, the average rate of elongation of newly-synthesised, 3H-thymidine-labelled daughter DNA strands, assessed by hydroxyapatite chromatography of alkali-denatured DNA was found to be normal. The results suggest that there is no impairment of DNA replication in the majority of the erythroblasts and that the abnormality of erythropoiesis resulted from disturbances during mitosis and the G2 phase. 相似文献
204.
Bielanski and Kaczmarski (1979) reported the presence of microtubules in the neck region of mature stallion spermatozoa. It was hypothesized that these microtubules are derived from the manchette (a microtubular organelle present during spermiogenesis). In order to test this hypothesis, testes from 15 mature stallions were collected, perfused with 2% phosphatebuffered glutaraldehyde, and prepared for transmission electron microscopy. Spermatozoa from the caudae epididymides of each stallion were prepared in a similar manner. Spermiogenesis was observed in general, and the presence of a microtubular manchette was established in this species, juxtapositioned posterior to the nuclear ring and extending distally into the cytoplasmic collar which surrounds the prospective midpiece. Interlocking arms between the microtubules of the manchette were observed in transverse sections at all levels within the cytoplasmic collar before, during, and after caudal migration of the nuclear ring. Consequent to caudal migration of the nuclear ring and the annulus, as well as adluminal movement of the spermatid, the cytoplasmic collar was transformed into the residual cytoplasm. Within the residual cytoplasm, the manchette remained as a microtubular organelle which undergoes degeneration. The mature spermatozoa from the caudae epididymides of these stallions lacked the microtubules reported by Bielanski and Kaczmarski. The occurrence of such microtubules in the neck region of stallion spermatozoa is probably an abnormality. 相似文献
205.
206.
207.
Myelotoxicity of serum and spinal fluid in multiple sclerosis: a critical assessment 总被引:1,自引:0,他引:1 下载免费PDF全文
Explants of neonatal rat cerebellum have been cultured on rat-tail collagen coated cover-slips for 2–4 weeks under conditions which yielded well myelinated nerve fibres suitable for testing myelotoxicity of serum. Eighty-four per cent of twenty-five acute multiple sclerosis serum samples were myelotoxic, as opposed to 62% of twenty-six sera from cases of motor neurone disease. Twenty-four per cent of thirty-four normal sera also showed distinct myelotoxicity. The difficulties in setting up and interpreting tests for in vitro demyelinating activity of serum are discussed. Six spinal fluids from acute cases of multiple sclerosis were without activity. It is concluded that although myelotoxicity exists in most samples of serum from acute multiple sclerosis, it is not limited to such subjects and that its high incidence in motor neurone disease sera indicates that it may well be a consequence of myelin destruction rather than a primary factor in its causation. 相似文献
208.
Homeostasis and the age-associated defect of CD4 T cells 总被引:1,自引:0,他引:1
Survival and homeostatic division of naive CD4 T cells is regulated by the cellular and non-cellular milieu and together these processes ensure that a population of naive CD4 T cells persists into old age. However, the naive CD4 T cells from aged animals show reduced IL-2 production, proliferation, helper function and effector generation and memory function. We explore here whether the age-related defects in naive CD4 T cells are due to the aged environment from which they come or to intrinsic defects that are caused by homeostasis and their long lifespan. 相似文献
209.
Owens EB Hinshaw SP Kraemer HC Arnold LE Abikoff HB Cantwell DP Conners CK Elliott G Greenhill LL Hechtman L Hoza B Jensen PS March JS Newcorn JH Pelham WE Severe JB Swanson JM Vitiello B Wells KC Wigal T 《Journal of consulting and clinical psychology》2003,71(3):540-552
Using receiver operating characteristics, the authors examined outcome predictors (variables associated with outcome regardless of treatment) and moderators (variables identifying subgroups with differential treatment effectiveness) in the Multimodal Treatment Study of Children with Attention-Deficit/Hyperactivity Disorder (ADHD; MTA). Treatment response was determined using parent- and teacher-reported ADHD and oppositional defiant symptoms, with levels near or within the normal range indicating excellent response. Among 9 baseline child and family characteristics, none predicted but 3 moderated treatment response. In medication management and combined treatments, parental depressive symptoms and severity of child ADHD were associated with decreased rates of excellent response; when these 2 characteristics were present, below-average child IQ was an additional moderator. No predictors or moderators emerged for behavioral and community comparison treatments. The authors discuss conceptual and clinical implications of research on treatment moderators. 相似文献
210.
Schild LJ Phillips DH Osborne MR Hewer A Beland FA Churchwell MI Brown K Gaskell M Wright E Poirier MC 《Mutagenesis》2005,20(2):115-124
Liver homogenates from rats fed tamoxifen (TAM) in the diet were shared among four different laboratories. TAM-DNA adducts were assayed by high pressure liquid chromatography-electrospray tandem mass spectrometry (HPLC-ES-MS/MS), TAM-DNA chemiluminescence immunoassay (TAM-DNA CIA), and (32)P-postlabeling with either thin layer ((32)P-P-TLC) or liquid chromatography ((32)P-P-HPLC) separation. In the first study, rats were fed a diet containing 500 p.p.m. TAM for 2 months, and the values for measurements of the (E)-alpha-(deoxyguanosin-N(2)-yl)-tamoxifen (dG-N(2)-TAM) adduct in replicate rat livers varied by 3.5-fold when quantified using 'in house' TAM-DNA standards, or other approaches where appropriate. In the second study, rats were fed 0, 50, 250 or 500 p.p.m. TAM for 2 months, and TAM-DNA values were quantified using both 'in house' approaches as well as a newly synthesized [N-methyl-(3)H]TAM-DNA standard that was shared among all the participating groups. In the second study, the total TAM-DNA adduct values varied by 2-fold, while values for the dG-N(2)-TAM varied by 2.5-fold. Ratios of dG-N(2)-TAM:(E)-alpha-(deoxyguanosin-N(2)-yl)-N-desmethyltamoxifen (dG-N(2)-N-desmethyl-TAM) in the second study were approximately 1:1 over the range of doses examined. The study demonstrated a remarkably good agreement for TAM-DNA adduct measurements among the diverse methods employed. 相似文献