头风痛丸质量标准

目的:建立头风痛丸的质量标准。方法:采用薄层色谱法(TLC)对头风痛丸中的川芎进行定性鉴别,用高效液相色谱法(HPLC)测定白芷中欧前胡素的含量。结果:TLC法能检出川芎,且斑点清晰,分离度好,易于识别;欧前胡素在0.323 2～1.616μg与峰面积呈良好线性关系(r=0.999 9),平均回收率为98.0%(RSD 0.84%)。结论:该方法简便、可靠,可用于头风痛丸的质量控制。     

Effect of dentin porcelain thickness on the shade of PFM

目的 探讨牙本质瓷厚度对金属烤瓷修复体颜色的影响.方法 用SHOFU Vintage Halo A2色牙本质瓷粉制作牙本质瓷为不同厚度(0.3mm、0.6mm、0.9mm、1.2mm、1.5mm)的金属烤瓷试件,用色度计测量试件的L*、a*、b*值,并计算其⊿E 和C*ab.比较试件颜色与VITA A2色比色片颜色的差异.结果 随着牙本质瓷厚度的增加,金属烤瓷试件的明度L*值逐渐下降,而a*、b*值逐渐升高,当厚度为1.2mm～1.5mm时与比色片的颜色无明显差异.结论 牙本质瓷厚度的变化对金属烤瓷修复体的颜色有显著影响.牙本质瓷只有在一定厚度范围才能达到与所选比色片颜色相一致的效果.     

牙本质瓷厚度对Ni-Cr合金烤瓷修复体颜色的影响

目的 探讨牙本质瓷厚度对金属烤瓷修复体颜色的影响.方法 用SHOFU Vintage Halo A2色牙本质瓷粉制作牙本质瓷为不同厚度(0.3mm、0.6mm、0.9mm、1.2mm、1.5mm)的金属烤瓷试件,用色度计测量试件的L*、a*、b*值,并计算其⊿E 和C*ab.比较试件颜色与VITA A2色比色片颜色的差...     

Juvenile Hyaline Fibromatosis: An Expanded Clinicopathologic Spectrum

A female infant had the typical cutaneous and soft tissue lesions of juvenile hyaline fibromatosis with the characteristic histologic and ultrastructural features of the disease. Her clinical course and autopsy findings strongly resembled those of infantile systemic hyalinosis, suggesting that the two conditions may be parts of a spectrum.     

Effects of extracellular matrices on human keratinocyte adhesion and growth and on its secretion and deposition of fibronectin in culture

Extracellular matrices (ECMs) play an important role as components of basement membrane of normal human skin and in migrating epidermal cells in wound healing. We investigated the effects of various ECMs on human keratinocyte adhesion and growth as well as on its secretion and deposition of fibronectin (FN) in vitro using a serum-free, low-calcium culture system. Since cell adhesion is the first step of cell growth, we performed cell adhesion assay for 14 h. Human keratinocytes adhered best on FN and less well on types I/III collagen, type IV collagen, and heparan sulfate proteoglycan (HSPG) as compared with bovine serum albumin (BSA) (control) or laminin (La). Cell growth assayed for 7-8 days on the dishes coated with various extracellular matrices revealed significantly increased keratinocyte growth on FN and on types I/III collagen in comparison with that on type IV collagen, HSPG, BSA (control), or La. Morphology of keratinocytes and of their colonies on FN and types I/III collagen was strikingly different from that of the control; the colonies were not so compact as in the control, but rather loose and larger; each keratinocyte was spread out more on these substrata. These morphologic features seemed to correlate with the increased keratinocyte growth on these extracellular matrices. Both immunofluorescence study for FN with keratinocytes in 8-day culture on various extracellular matrices and enzyme-linked immunosorbent assay for FN measurement on substratum or in conditioned medium with keratinocytes in 5-day culture demonstrated that extracellular matrices modulated the secretion and deposition of FN by human keratinocytes in culture; the keratinocyte growth correlated with the amount of FN detected on substratum but not with that in medium. Based on the results of the present investigation, we think that the growth of human keratinocytes depends on the amount of FN on substratum.     

Effect of activated human mast cells and mast cell-derived mediators on proliferation,type I collagen production and glycosaminoglycans synthesis by human dermal fibroblasts

Although an increased number of mast cells in fibrotic tissues such as scleroderma, keloid or healing wound has been highlighted, it is still unclear whether or not mast cells are fibrogenic. The aim of the present study is to determine whether functionally active human mast cells can provide human dermal fibroblasts directly with fibrogenic properties. In order to examine the effects of IgE-mediated mast cell activation on fibroblast proliferation and synthesis of type I collagen, we utilized an in vitro defined system in which cultured human mast cells were co-cultured with human dermal fibroblasts. We also employed a three-dimensional fibroblast culture system using supplementation of L-ascorbic acid as an assay system to investigate the effects of mast cell-derived mediators on synthesis of glycosaminoglycans by human fibroblast. Fibroblast proliferation was actively stimulated with IgE-activated mast cells. However, this stimulatory effect was canceled in co-cultures with a higher number of IgE-activated mast cells. In the presence of a higher number of activated mast cells, the fibroblast cell layer was destroyed, in contrast to an intact cell layer in the presence of same number of the mast cells without activation. Type I collagen synthesis was unchanged in fibroblasts co-cultured with mast cells. The total amount of main disaccharide units, particularly DELTADi-HA, was increased when fibroblasts were exposed to histamine. Thus, we conclude that other factors, in addition to mast cells, are important in the development of human tissue fibrosis or sclerosis.     

肱二头肌超声参数联合临床资料诊断慢性肾脏病患者蛋白质能量消耗的临床价值

目的 应用超声测量肱二头肌相关定量参数,探讨其联合临床资料诊断慢性肾脏病(CKD)患者蛋白质能量消耗(PEW)的临床价值。方法 选取于我院肾内科血液净化中心接受血液透析治疗的CKD患者83例,其中未合并PEW者18例(对照组),合并PEW者65例(异常组);比较两组临床资料及肱二头肌超声定量参数的差异;应用多因素Logistic回归分析CKD患者PEW的独立影响因素,并建立联合诊断模型;绘制受试者工作特征(ROC)曲线分析该模型预测CKD患者PEW的诊断效能。结果 两组体质量指数(BMI)、透析前血肌酐(Cr)、肱三头肌皮褶厚度(TSF)、上臂中段肌围(AMC),以及超声所测肱二头肌舒张状态纵径、舒张状态面积、舒张状态与收缩状态纵径比值比较差异均有统计学意义(均P<0.05)。多因素Logistic回归分析显示,BMI、肱二头肌舒张状态与收缩状态纵径比值均为CKD患者发生PEW的独立影响因素(OR=0.733、9.028,均P<0.05)。建立联合诊断模型为：Logit(P)=1/[1+e⁽-(8.228-0.310×BMI+2.200×肱二头肌舒张状...     

食管全周浅表癌内镜黏膜下剥离术后长期保留胃管对食管狭窄的预防及治疗作用

为探讨食管全周浅表癌行内镜黏膜下剥离术（endoscopic submucosal dissection,ESD）后长期保留胃管对食管狭窄预防及治疗的影响,回顾性分析2018年1月—2021年12月在南京医科大学第一附属医院行ESD的食管全周浅表癌患者,术后置入胃管患者15例（胃管置入组）,无胃管置入患者23例（无胃管置入组）,比较两组患者基础情况、病变位置、病理分期、术后并发症、食管狭窄程度（进水情况）、疼痛情况、住院次数及医疗费用等资料。结果显示,两组患者在年龄、性别、病变位置及术后病理分期的构成方面差异无统计学意义（P>0.05）。与无胃管置入组相比,胃管置入组患者可进水率较高（11/15比6/23,P<0.05）,发生疼痛次数较少［（7.3±3.1）次比（10.7±3.6）次,t=3.00,P<0.05］,ESD后至食管支架置入前及置入后的住院次数和医疗总费用均明显低于无胃管置入组（P<0.05）。ESD后迟发性出血率、穿孔率以及首次狭窄出现时间,两组差异均无统计意义（P>0.05）。研究结果初步表明食管全周病变患者行ESD后长期保留胃管可减轻食管狭窄程度,具有较良好的安全性。     

颗粒蛋白前体衍生物 Atsttrin对骨关节炎的保护作用及对 CD+4T细胞的调节作用

目的 揭示颗粒蛋白前体衍生物Atsttrin对骨关节炎的保护作用及对CD+4 T细胞的调节作用.方法 本研究起止时间为2018年10月至2019年12月,60只5周龄SD雄性大鼠购自西安交通大学医学部实验动物中心.采用膝关节前交叉韧带横断法建立骨关节炎大鼠模型,通过关节内注射15μL Atsttrin(1μg/μL)对模型大鼠进行治疗,每周注射1次,连续注射4周.通过番红O固绿染色检测软骨损伤程度,采用OARSI评分系统评价软骨退化情况.ELISA法检测大鼠血清干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)、白细胞介素-17(IL-17)和转化生长因子-β(TGF-β)水平.流式细胞仪分析血清Th1、Th2、Th17和Treg细胞比例.应用终浓度为10μg/L的肿瘤坏死因子-α(TNF-α)诱导滑膜细胞和软骨细胞48 h,并分别用终浓度为10μg/L、20μg/L、50μg/L的Atsttrin处理滑膜细胞和软骨细胞48 h.通过5-溴-2-脱氧尿苷(BrdU)细胞增殖测定试剂盒检测滑膜或软骨细胞的增殖.通过RT-PCR和Western blotting检测软骨细胞中解聚蛋白样金属蛋白酶-4(ADAMTS-4)、基质金属蛋白酶-13(MMP-13)、IFN-γ、IL-4、IL-17和TGF-β的信使核糖核酸(mRNA)和蛋白表达.结果 与模型组相比,Atsttrin组大鼠的膝关节软骨破坏情况明显减轻(P<0.05).与模型组相比,Atsttrin组大鼠国际骨关节炎研究学会(OARSI)评分显著降低[(2.56±0.21)比(1.08±0.11),P=0.001].Atsttrin组大鼠的血清IFN-γ和IL-17水平显著低于模型组,而IL-4和TGF-β水平显著高于模型组(P<0.05).Atsttrin组大鼠的血清Th1和Th17细胞比例显著低于模型组,而Th2和Treg细胞比例显著高于模型组(P<0.05).Atst?trin处理显著降低了TNF-α诱导的滑膜细胞的增殖能力,并提高了TNF-α诱导的软骨细胞的增殖能力(P<0.05).Atsttrin显著下调了TNF-α诱导的软骨细胞中ADAMTS-4、MMP-13、IFN-γ和IL-17的mRNA和蛋白表达水平,并上调了IL-4和TGF-β的表达水平(P<0.05).结论 Atsttrin可有效抑制骨关节炎大鼠模型的软骨病变并提高关节功能.Atsttrin可通过抑制滑膜细胞增殖、促进软骨细胞增殖、抑制软骨机制降解来维持关节内微环境的稳定.此外,Atsttrin的关节软骨保护作用与纠正Th1/Th2细胞以及Th17/Treg细胞的失衡有关.     

老年睑板腺功能障碍蠕形螨感染率及茶树精油联合氟米龙的疗效

目的：研究老年睑板腺功能障碍(MGD)蠕形螨感染率及茶树精油联合氟米龙的疗效。

方法：回顾性病例对照研究。选择2017-09/2018-03在宁波市眼科医院门诊就诊的老年MGD患者59例118眼。将睫毛蠕形螨镜检为阳性的38例患者76眼进行症状评分、蠕形螨数量、泪膜破裂时间(BUT)、角膜荧光素染色(FL)、Schirmer I试验(SIaT)等检查,并按不同治疗方法分成3组,A组使用0.02%氟米龙滴眼液滴眼24眼; B组使用茶树精油(TTO)湿巾敷眼26眼; C组用0.02%氟米龙滴眼液滴眼联合TTO湿巾敷眼26眼,随访4wk。

结果：118眼中76眼(64.4%)睫毛蠕形螨镜检为阳性。睫毛蠕形螨阳性的三组患者治疗后的主观症状评分均较治疗前有改善(P<0.05)。治疗后A组蠕形螨数量较治疗前无显著差异(P=0.11); B、C组较治疗前明显减少(均P<0.01)。三组患者BUT治疗前后比较,A组BUT无显著差异(P=0.22); B、C组BUT明显延长(均P<0.05)。治疗后C组的BUT较A、B组明显延长(均P<0.05)。三组患者治疗前后的FL评分均较治疗前明显改善(P<0.05)。

结论：老年MGD患者睫毛蠕形螨感染率较高,睑板腺热疏通后TTO湿巾联合0.02%氟米龙滴眼液能有效驱螨、缓解MGD患者局部症状。