iTRAQ‐based proteomic analysis of differentially expressed proteins in sera of seronegative and seropositive rheumatoid arthritis patients

ObjectiveThe diagnosis of seronegative rheumatoid arthritis (SNRA) is often difficult due to the unavailability of reliable laboratory markers. The aim of this study was to identify differentially expressed proteins in sera of SNRA, seropositive RA (SPRA), and healthy donors (HD).MethodsA total of 32 seropositive RA patients, 32 SNRA patients, and 35 HD were enrolled in our study. Differentially expressed proteins between 3 groups were identified via isobaric tags for relative and absolute quantitation (iTRAQ)‐based proteomic analysis, and an ELISA test was used for the validation test. Correlation analysis was conducted by GraphPad Prism.ResultsUsing iTRAQ quantitative proteomics, we identified 14 proteins were significantly different between SPRA and SNRA, including 4 upregulated proteins and 10 downregulated proteins. Four differentially expressed proteins were validated by ELISA test, and the results showed that SAA1 protein was significantly higher in SPRA and SNRA patients compared with HD, and PSME1 was elevated in SPRA patients. What''s more, SAA1 was increased in the anti‐CCP or RF high‐level group in RA patients, and PSME1 was increased in the RF high‐level group. Alternatively, SAA1 was positively correlated with inflammation indicators in RA patients, while PSME1 showed no correlation with inflammation indicators.ConclusionsiTRAQ proteomic approaches revealed variations in serum protein composition among SPRA patients, SNRA patients, and HD and provided new idea for advanced diagnostic methods and precision treatment of RA.     

自噬介导的STEMI心肌缺血-再灌注损伤中PAR-2、Bcl-2、Beclin1的表达及其相关性

目的:探究自噬介导的急性ST段抬高型心肌梗死(STEMI)心肌缺血再灌注损伤中蛋白酶激活受体-2(PAR-2)、B细胞淋巴瘤因子-2(Bcl-2)、人抑癌基因Beclin1的表达及其相关性.方法:选取2017年1月至2018年7月诊断为STEMI患者50例为试验组,有胸痛症状但冠状动脉造影检查正常者50例为对照组,检测...     

Metallurgical and interfacial characterization of PFM Co–Cr dental alloys fabricated via casting,milling or selective laser melting

Objectives

Bulk and interfacial characterization of porcelain fused to metal (PFM) Co–Cr dental alloys fabricated via conventional casting, milling and selective laser melting.

Methods

Three groups of metallic specimens made of PFM Co–Cr dental alloys were prepared using casting (CST), milling (MIL) and selective laser sintering (SLM). The porosity of the groups was evaluated using X-ray scans. The microstructures of the specimens were evaluated via SEM examination, EDX and XRD analysis. Vickers hardness testing was utilized to measure the hardness of the specimens. Interfacial characterization was conducted on the porcelain-covered specimens from each group to test the elemental distribution with and without the application of INmetalbond. The elemental distribution of the probed elements was assessed using EDX line profile analysis. Hardness results were statistically analyzed using one-way ANOVA and Holm–Sidak's method (α = 0.05).

Results

X-ray radiography revealed the presence of porosity only in the CST group. Different microstructures were identified among the groups. Together with the γ phase matrix, a second phase, believed to be the Co₃Mo phase, was also observed by SEM and subsequent XRD analysis. Cr₇C₃ and Cr₂₃C₆ carbides were also identified via XRD analysis in the CST and MIL groups. The hardness values were 320 ± 12 HV, 297 ± 5 HV and 371 ± 10 HV, and statistically significant differences were evident among the groups.

Significance

The microstructure and hardness of PFM Co–Cr dental alloys are dependent on the manufacturing technique employed. Given the differences in microstructural and hardness properties among the tested groups, further differences in their clinical behavior are anticipated.     

Identification of an IDO1‐based immune classifier for survival prediction of upper tract urothelial carcinoma

The limited response rate of immunotherapy in upper tract urothelial carcinoma (UTUC) might be attributed to additional immunosuppressive mechanisms in vivo. As a promising immune checkpoint target, the expression and prognostic role of indoleamine 2,3‐dioxygenase 1 (IDO1) in UTUC remains unknown. In this study, the expression and prognostic value of IDO1 was analyzed in 251 patients from 3 independent cohorts. The least absolute shrinkage and selection operator (LASSO) Cox regression model was used to construct an IDO1‐based immune classifier and external validation was performed to further validate the classifier. RNA sequencing and immunofluorescence were used to explore the immune contexture of different risk groups stratified by classifier. We found that high IDO1 expression on tumor cells (TC) indicated a poorer overall survival and disease‐free survival in all cohorts. Patients with high expression of IDO1 TC possessed increased infiltration of CD4⁺, CD8⁺ and Foxp3⁺ T cells. An immune classifier based on intratumoral CD8⁺ lymphocytes, IDO1 TC, and stromal PD‐L1 expression status was developed, with its area under the curves (AUCs) values for overall survival at 5 y being 0.79 (95% confidence interval [CI] 0.65‐0.93) in the discovery cohort, 0.75 (95% CI 0.58‐0.92) and 0.78 (95% CI 0.65‐0.92) in the internal and external validation cohorts, respectively. The high‐risk group stratified by the immune classifier was associated with immunosuppressive contexture, accompanied by enhanced CD8⁺ T cells exhaustion patterns. Our IDO1‐based immune classifier can provide a superior accuracy for survival prediction and lead to individual stratification of UTUC immune subtypes.     

Development and Validation of a Metabolic-related Prognostic Model for Hepatocellular Carcinoma

Background and Aims: Growing evidence suggests that metabolic-related genes have a significant impact on the occurrence and development of hepatocellular carcin...     

整合素和粘着斑激酶在JWA基因抑制诱导的人肺动脉平滑肌细胞迁移调控中的作用

目的探讨整合素和粘着斑激酶是否在JWA基因抑制诱导的人肺动脉平滑肌细胞（PASMCs）迁移调控中起作用。方法前期实验将JWA核酶基因构建到逆转录病毒载体pLXSN上，转染人体外培养的人PASMCs内，并证实转染的JWA特异性核酶基因能抑制PASMCs内JWA基因的表达。该研究应用改良Boyden小室法测定细胞迁移情况．半定量RT-PCR（sqRT-PCR）法测定细胞内整合素αv、整合素β3。粘着斑激酶的mRNA表达量．Western blot法检测细胞内整合素αv、整合素β3、粘着斑激酶的蛋白表达量。结果与空载体转染细胞（P-pLXSN组）及未转染细胞（PASMCs组）比较．转染JWA核酶基因的细胞（P-JWARZ组）迁移率显著增加，细胞内整合素αv、整合素β3、粘着斑激酶的mRNA及蛋白表达量均显著增加。结论整合素、粘着斑激酶信号通路可能在JWA基因抑制诱导的人肺动脉平滑肌细胞迁移中起调控作用。     

Pancreaticocolonic fistula: a complication of pancreatitis.

In three cases of pancreaticocolonic fistula presenting before the stage of exsanguinating hemorrhage of severe sepsis the problem was diagnosed on the basis of the clinical history, visualization of the terminal part of the fistula by roentgenography after a barium enema had been given and, in two cases, demonstration of the communication with the pancreatic ductal system by endoscopic retrograde pancreatography. The lesions were repaired surgically. Pancreaticocolonic fistula should be suspected in a patient with upper abdominal pain who has a history of abdominal pain and excessive alcohol consumption and in whom diarrhea and fever, hematochezia or a disappearing abdominal mass develops. Characteristically barium will collect in the terminal part of the fistula and thus permit a tentative diagnosis; the diagnosis can then be confirmed by endoscopic retrograde pancreatography. With this approach surgical treatment can be carried out earlier and the often fatal course of the disorder can be averted.     

Noscapine suppresses angiotensin converting enzyme inhibitors-induced cough

BACKGROUND: Dry cough is a common side-effect of the angiotensin converting enzyme inhibitors (ACEI) and is a major limiting factor of their use. It has been suggested that ACEI cause this side-effect by potentiation of the bradykinin effect. Previous work in our laboratory has shown that noscapine, an antitussive drug, inhibits the effect of bradykinin. METHODS: To investigate the effect of noscapine on ACEI-induced cough, 611 hypertensive patients who were being treated with ACEI were evaluated for the incidence of persistent dry cough. RESULTS: A cough had developed in 65 (10.6%) patients, two (3.1%) of whom also had severe respiratory distress that required hospitalisation and immediate discontinuation of the ACEI. Forty-two (64.6%) patients had developed a mild cough and 21 (32.3%) patients had developed a moderate to severe cough. The patients with moderate to severe cough received 15 mg of noscapine, orally three times daily, while they continued ACEI. Noscapine effectively resolved the cough in 19 (90%) patients within 4-9 days of starting treatment. CONCLUSION: Noscapine, possibly by inhibition of bradykinin synthesis, eliminates ACEI-induced cough in the majority of patients and allows them to continue with ACEI therapy.     

肺大泡自发性气胸手术麻醉处理：—附28例临床分析

目的：探讨肺大泡自发气胸病人在麻醉期间的危险性及预防措施，方法：为了预防麻醉诱导和维持期间的肺大泡破裂，本组麻醉诱导面罩吸氧应用法控制呼吸囊辅助通气和控制通气时，轻压呼吸囊，使胸廓略有起伏，血氧饱和度在基础状态下逐渐上升，同时监测气道压力，使气道压不超过20cmH2O(1.96kPa)，以SpO2平稳上升为手法控制呼吸囊的适宜程度，同时选用双腔管插管。结果：本组28例肺大泡自发性气胸在麻醉期间无1例出现麻醉期间肺大泡破裂。结论：对此类病人选用全麻快速诱导，双腔管插管，手法控制通气量，严密监测SpO2和气道压，可防止肺大泡破裂。