全文获取类型
收费全文 | 5795篇 |
免费 | 387篇 |
国内免费 | 40篇 |
专业分类
耳鼻咽喉 | 84篇 |
儿科学 | 147篇 |
妇产科学 | 130篇 |
基础医学 | 838篇 |
口腔科学 | 615篇 |
临床医学 | 521篇 |
内科学 | 1121篇 |
皮肤病学 | 163篇 |
神经病学 | 497篇 |
特种医学 | 66篇 |
外科学 | 508篇 |
综合类 | 31篇 |
一般理论 | 1篇 |
预防医学 | 630篇 |
眼科学 | 111篇 |
药学 | 486篇 |
中国医学 | 47篇 |
肿瘤学 | 226篇 |
出版年
2024年 | 9篇 |
2023年 | 77篇 |
2022年 | 161篇 |
2021年 | 286篇 |
2020年 | 194篇 |
2019年 | 249篇 |
2018年 | 301篇 |
2017年 | 177篇 |
2016年 | 235篇 |
2015年 | 251篇 |
2014年 | 336篇 |
2013年 | 328篇 |
2012年 | 541篇 |
2011年 | 605篇 |
2010年 | 300篇 |
2009年 | 237篇 |
2008年 | 342篇 |
2007年 | 375篇 |
2006年 | 272篇 |
2005年 | 231篇 |
2004年 | 183篇 |
2003年 | 120篇 |
2002年 | 144篇 |
2001年 | 41篇 |
2000年 | 32篇 |
1999年 | 31篇 |
1998年 | 19篇 |
1997年 | 9篇 |
1996年 | 14篇 |
1995年 | 7篇 |
1994年 | 11篇 |
1993年 | 6篇 |
1992年 | 11篇 |
1991年 | 5篇 |
1990年 | 11篇 |
1989年 | 4篇 |
1988年 | 3篇 |
1987年 | 5篇 |
1986年 | 7篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1981年 | 5篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1978年 | 4篇 |
1976年 | 3篇 |
1975年 | 4篇 |
1974年 | 5篇 |
1973年 | 3篇 |
1968年 | 4篇 |
排序方式: 共有6222条查询结果,搜索用时 15 毫秒
71.
BACKGROUND: Data on patients with cancer who have a prolonged length of stay (LOS) in the ICU are scarce. The aim of the present study was to evaluate the characteristics and the outcomes of cancer patients with life-threatening complications with an ICU stay >/= 21 days. METHODS: A cohort study performed at a 10-bed oncology medical-surgical ICU from May 2000 to December 2005. Prolonged ICU LOS was defined as an ICU stay >/= 21 days. RESULTS: During the period, 1,090 patients were admitted to the ICU and 163 patients (15%) had a prolonged ICU LOS. These patients, however, accounted for 48% (5,828/12,224) of the total ICU bed-days. The hospital and 6-month mortality rates were 50% and 60%, respectively, and similar to patients with ICU LOS < 21 days (51% and 61%, respectively). ICU-acquired events and complications were common, and the most frequent were infections (90%), mechanical ventilation (99%), and need for vasopressors (88%). The number of organ failures, older age, and poor performance status were the main outcome predictors. The median long-term follow-up after hospital discharge was 537 days (range, 193 to 1,119 days), and 29 patients (18%) were alive. CONCLUSIONS: Fifteen percent of critically ill patients with cancer had a prolonged ICU LOS. Short- and long-term survival rates were reasonable, and the prognosis was better than expected a priori. In our opinion, the length of ICU admission per se should not be used in the clinical decisions regarding the continuation of treatment in these patients. 相似文献
72.
Mário Sérgio Lima de Lavor Nancy Scardua Binda Fabíola Bono Fukushima Fátima Maria Caetano Caldeira Juliana Figueira da Silva Carla Maria Osório Silva Karen Maciel de Oliveira Bernardo de Caro Martins Bruno Benetti Junta Torres Isabel Rodrigues Rosado Renato Santiago Gomez Marcus Vinícius Gomez Eliane Gon?alves de Melo 《International journal of clinical and experimental pathology》2015,8(9):9941-9949
This work aimed at determining the ideal ischemia time in an in vitro ischemia-reperfusion model of spinal cord injury. Rat spinal cord slices were prepared and then exposed or not to oxygen deprivation and low glucose (ODLG) for 30, 45, 60, 75 and 90 minutes. Cell viability was assessed by triphenyltetrazolium (TTC), lactate dehydrogenase (LDH) release, and fluorochrome dyes specific for cell dead (ethidium homodimer) using the apotome system. Glutamate release was enzymatically measured by a fluorescent method. Gene expression of apoptotic factors was assessed by real time RT-PCR. Whereas spinal cord slices exposed to ODLG exhibited mild increase in fluorescence for 30 minutes after the insult, the 45, 60, 75 and 90 minutes caused a 2-fold increase. ODLG exposure for 45, 60, 75 or 90 minutes, glutamate and LDH release were significantly elevated. nNOS mRNA expression was overexpressed for 45 minutes and moderately increased for 60 minutes in ODLG groups. Bax/bcl-xl ratio, caspase 9 and caspase 3 mRNA expressions were significantly increased for 45 minutes of ODLG, but not for 30, 60, 75 and 90 minutes. Results showed that cell viability reduction in the spinal cord was dependent on ischemic time, resulting in glutamate and LDH release. ODLG for 45 minutes was adequate for gene expression evaluation of proteins and proteases involved in apoptosis pathways. 相似文献
73.
74.
Marie-Luise Berres Karen Phaik Har Lim Tricia Peters Jeremy Price Hitoshi Takizawa Hélène Salmon Juliana Idoyaga Albert Ruzo Philip J. Lupo M. John Hicks Albert Shih Stephen J. Simko Harshal Abhyankar Rikhia Chakraborty Marylene Leboeuf Monique Beltr?o Sérgio A. Lira Kenneth M. Heym Bj?rn E. Clausen Venetia Bigley Matthew Collin Markus G. Manz Kenneth McClain Miriam Merad Carl E. Allen 《The Journal of experimental medicine》2015,212(2):281
75.
76.
77.
Ana Teresa P Carvalho Barbara C Esberard Renata S B Fróes Davy C M Rapozo Ana B Grinman Tatiana A Simo Juliana C V C Santos Antonio José V Carneiro Luis Felipe Ribeiro-Pinto Heitor S P de Souza 《World journal of gastroenterology : WJG》2014,20(12):3327-3334
AIM:To analyze the prevalence of thiopurine-methyltransferase(TPMT)genotypes and their associationwith drug toxicity in inflammatory bowel disease(IBD)patients from southeastern Brazil.METHODS:A total of 219 consecutive patients with IBD,of which 146 had Crohn’s disease and 73 had ulcerative colitis,regularly seen at the outpatient unit of the Division of Gastroenterology at the University Hospital Pedro Ernesto of the State University of Rio de Janeiro,a tertiary referral center,were enrolled in this study from February 2009 to January 2011.We analyzed the presence of major TPMT genetic variants(TPMT*2,*3A,*3C)in IBD patients by means of a specific allele and RFLP-PCR.Genomic DNA was isolated from peripheral blood leukocytes by proteinase-K/Sodium Dodecyl Sulfate digestion and phenol-chloroform extraction.TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes were detected by real-time polymerase chain reaction followed by direct sequencing with specific primers.Clinical data were systematically recorded,and correlated with the genotype results.RESULTS:The distribution of the selected TPMT gene polymorphism TPMT*2(C238G),TPMT*3A(G460A/A719G),and TPMT*3C(A719G)genotypes was 3.6%,5.4%,and 7.7%of the patients,respectively.Among the side effects recorded from patients taking azathioprine,14 patients presented with pancreatitis and/or an elevation of pancreatic enzymes,while 6 patients had liver toxicity,and 2 patients exhibited myelosuppression/neutropenia.TPMT polymorphisms were detected in 37/219 patients(8 heterozygous for*2,11 heterozygous for*3A,and 18 heterozygous for*3C).No homozygotic polymorphisms were found.Despite the prevalence of the TPMT*3C genotype,no differences among the genotype frequencies were significant.Although no association was detected regarding myelotoxicity or hepatotoxicity,a trend towards the elevation of pancreatic enzymes was observed for TPMT*2 and TPMT*3C genotypes.CONCLUSION:The prevalence of TPMT genotypes was high among Brazilian patients.Variants genes*2and*3C may be associated with azathioprine pancreatic toxicity in a IBD southeastern Brazilian population. 相似文献
78.
79.
Lindisley F. Gomides Onésia C. O. Lima Natália A. Matos Kátia M. Freitas Janetti Nogueira Francischi Juliana Carvalho Tavares André Klein 《Inflammation research》2014,63(11):935-941
Objective and design
The activation of proteinase-activated receptors (PARs) has been implicated in the development of important hallmarks of inflammation, including in vivo leukocyte recruitment; however, its role in the regulation of leukocyte migration in response to inflammatory stimuli has not been elucidated until now. Here, we examined the effects of the PAR4 antagonist YPGKF-NH 2 (tcY-NH2) on neutrophil recruitment in experimentally induced inflammation.Methods
BALB/c mice were intrapleurally injected with tcY-NH2 (40 ng/kg) prior to intrapleural injection of carrageenan (Cg) or neutrophil chemoattractant CXCL8; the number of infiltrating neutrophils was evaluated after 4 h, and KC production was assessed at different times after Cg injection. Neutrophil adhesion and rolling cells were studied using a brain circulation preparation 4 h after the Cg or CXCL8 challenge in tcY-NH2-treated mice.Results
PAR4 blockade inhibited CXCL8- and Cg-induced neutrophil migration into the pleural cavity of BALB/c mice and reduced neutrophil rolling and adherence. Surprisingly, PAR4 blockade increased the level of KC in response to carrageenan.Conclusion
These results demonstrated that PAR4 blockade impairs neutrophil migration in vivo, suggesting that PAR4 plays an important role in the regulation of inflammation, at least in part because of its ability to inhibit the actions of the neutrophil chemoattractant CXCL8. 相似文献80.
Anna Sessa Alessandra Mazzola Chetana Lim Mohammed Atif Juliana Pappatella Valerie Pourcher Olivier Scatton Filomena Conti 《World journal of gastroenterology : WJG》2020,26(44):7076-7084
BACKGROUNDCoronavirus disease (COVID) is a new and highly contagious infectious disease caused by the coronavirus (COVID-19 or severe acute respiratory syndrome coronavirus 2). There is limited data regarding the incidence and management of COVID-19 in immunocompromised patients’ post-transplantation. In the pre-COVID-19 era, these patients were already at an increased risk of developing opportunistic infections. These often manifested with atypical symptoms.CASE SUMMARYWe report another case of uneventful COVID-19 pneumonia in a 58-year old male who was 18 mo’ post liver transplantation. He received tacrolimus monotherapy since July 2019. The clinical manifestations included only epigastric pain radiating to the right hypochondrium, nausea and vomiting. He had no fevers, cough, shortness of breath, anosmia or dysgeusia even if the chest computed tomography scan revealed an extension of the multiple patchy ground-glass density shadows to the upper lobe of the left lung too. He was hospitalised and received a course of oral chloroquine (200 mg × 3 per day) for a period of 10 d. Interestingly, the COVID 19 infection was uneventful though there were no modifications to his tacrolimus dosing. He was successfully discharged. We performed subsequent follow-up via telemedicine.CONCLUSIONIn light of the current pandemic, it is even more important to identify how the liver recipient’s patients present and are managed, especially for immunosuppression treatment. 相似文献