转染特异性报导基因成骨细胞生物学性状及其与骨支架材料生物相容性的研究

目的研究转染特异性报导基因成骨细胞的生物学性状，并观察该细胞在骨支架材料上的生长特性和黏附性。方法常规复苏培养转染12×SBE—OC—Luc报导基因的前成骨细胞株OCTl细胞，通过倒置相差显微镜、HE染色、I型胶原免疫组化法染色、碱性磷酸酶（ALP）偶氮偶联法染色、矿化结节茜素红法染色及四环素法染色观察该转基因成骨细胞的生物学性状；并将其与纳米磷酸钙复合骨支架进行体外复合培养，通过扫描电镜观察该转基因成骨细胞在骨支架材料上的生长特性和黏附性。结果转染12×SBE—OC—Luc报导基因的前成骨细胞株OCTl细胞经培养后能贴壁生长，形态和成纤维细胞相似，能分泌胶原基质和ALP，经I型胶原免疫组化法染色及ALP偶氮偶联法染色呈强阳性；并能够形成矿化结节，茜素红法染色及四环素法染色呈阳性；该转基因成骨细胞在骨支架材料上具有良好的生长特性和黏附性，并能正常分化、增殖和成熟，分泌大量胶原基质和钙结节。结论转染12×SBE—OC—Luc报导基因后成骨细胞生物学性状未发生改变，其与骨支架材料亦具有良好的生物相容性。     

Performance measurement of the microPET focus 120 scanner.

The microPET Focus 120 scanner is a third-generation animal PET scanner dedicated to rodent imaging. Here, we report the results of scanner performance testing. METHODS: A (68)Ge point source was used to measure energy resolution, which was determined for each crystal and averaged. Spatial resolution was measured using a (22)Na point source with a nominal size of 0.25 mm at the system center and various off-center positions. Absolute sensitivity without attenuation was determined by extrapolating the data measured using an (18)F line source and multiple layers of absorbers. Scatter fraction and counting rate performance were measured using 2 different cylindric phantoms simulating rat and mouse bodies. Sensitivity, scatter fraction, and noise equivalent counting rate (NECR) experiments were repeated under 4 different conditions (energy window, 250 approximately 750 keV or 350 approximately 650 keV; coincidence window, 6 or 10 ns). A performance phantom with hot-rod inserts of various sizes was scanned, and several animal studies were also performed. RESULTS: Energy resolution at a 511-keV photopeak was 18.3% on average. Radial, tangential, and axial resolution of images reconstructed with the Fourier rebinning (FORE) and filtered backprojection (FBP) algorithms were 1.18 (radial), 1.13 (tangential), and 1.45 mm full width at half maximum (FWHM) (axial) at center and 2.35 (radial), 1.66 (tangential), and 2.00 mm FWHM (axial) at a radial offset of 2 cm. Absolute sensitivities at transaxial and axial centers were 7.0% (250 approximately 750 keV, 10 ns), 6.7% (250 approximately 750 keV, 6 ns), 4.0% (350 approximately 650 keV, 10 ns), and 3.8% (350 approximately 650 keV, 6 ns). Scatter fractions were 15.9% (mouse phantom) and 35.0% (rat phantom) for 250 approximately 750 keV and 6 ns. Peak NECR was 869 kcps at 3,242 kBq/mL (mouse phantom) and 228 kcps at 290 kBq/mL (rat phantom) at 250 approximately 750 keV and 6 ns. Hot-rod inserts of 1.6-mm diameter were clearly identified, and animal studies illustrated the feasibility of this system for studies of whole rodents and mid-sized animal brains. CONCLUSION: The results of this independent field test showed the improved physical characteristics of the F120 scanner over the previous microPET series systems. This system will be useful for imaging studies on small rodents and brains of larger animals.     

125I粒子组织间置入治疗骨转移瘤

目的探讨^125I粒子组织间永久置入在骨转移瘤中的治疗价值。方法对22例不同来源骨转移瘤患者采取^125I粒子永久置入的方法进行治疗，观察治疗后骨痛缓解及术后病灶影像改变的情况。结果^125I粒子永久置入治疗骨转移瘤止痛有效率为91％（20／22），术后2个月粒子置入病灶的影像检查结果显示，32个骨转移灶中，完全缓解（CR）4个、部分缓解（PR）18个、稳定（NC）10个，总缓解率68．7％，所有患者均未出现严重不良反应。结论组织间永久置入^125I粒子治疗骨转移瘤止痛效果好，不良反应发生率低且程度轻，是一种疗效较好的治疗方法。     

妊娠期肝内胆汁淤积症病人的护理

妊娠期肝内胆汁淤积症(intrahepatic cholestasis of prenancy,ICP)是妊娠期特有的一种并发症,多发生于妊娠中期、晚期,表现为皮肤瘙痒和黄疸,主要危害胎儿,可导致早产、羊水胎粪污染及胎儿宫内窘迫,甚至死胎.目前已受到产科临床工作者的高度重视.我院自2000年1月-2004年11月收治ICP病人173例,经过积极治疗、严密监护、适时终止妊娠,母婴情况良好.现将有关护理总结如下.……     

Molecular and Serological Evidence for Anaplasma platys and Babesia sp. Infection in a Dog,Imported in Belgium,from Southern Spain

This case report describes a dog suffering from a co‐infection with Babesia and Anaplasma parasites. Anaplasma platys was found to be responsible for the anaplasmosis by molecular biology techniques, while microscopical and serological evidence was found for a coexistent babesiosis, although this could not be confirmed by polymerase chain reaction. Moreover, the possible risk of import of exotic pathogens is highlighted.     

腰椎峡部裂并滑脱CT扫描技术与CT征象分析

1资料与方法本组共35例,男25例,女10例,年龄25~56岁。其中腰痛伴单或双侧下肢疼痛及麻木19例,单纯腰痛15例,骶尾部疼痛1例,均无急性外伤史。使用美国GE公司Pro-speedAI螺旋CT机,层厚3 mm,层距3 mm。20例采用常规扫描,即L3~S1三个椎间盘,对峡部裂椎体从椎体下缘向上按椎间盘扫描     

听神经病的纯音听阈检查结果分析

目的 探讨听神经病纯音听阈(PTT)检查的特征。方法 对48例听神经病患者的PTT检查结果进行分析，并与耳蜗性聋进行对照比较。结果 听神经病的PTT图有上升型、峰型、匙型、W型、倒S型、水平型和缓降型。双耳对称的PTT图有38例，不对称的有10例。听神经病的听阈升高程度呈轻、中度。左右耳听阈升高程度基本一致。低频听阈升高或以低频听阈升高为主的PTT图型占93．75％(90／96耳)。结论 双耳对称或基本对称的低频听阈升高或以低频听阈升高为主是听神经病的重要特征。     

诺新康与巴曲酶联用治疗进展性脑梗死的临床研究

目的观察诺新康联合巴曲酶治疗进展性脑梗死的临床疗效及其可能的作用机制。方法选择我院急性进展性脑梗死的患者为研究对象，随机分成两组，治疗组和对照组各26例，对照组予以常规治疗，治疗组在常规治疗的基础上加诺新康与巴曲酶。结果治疗组对神经功能缺损的治疗效果及控制梗死进展优于对照组。结论诺新康与巴曲酶联合对进展性脑梗死具有很好的治疗效果。     

软组织扩张中扩张囊内外的细菌学分析

目的研究软组织扩张术患者扩张囊内、外的细菌数量及种类，为临床上防治扩张术感染提供依据。方法连续采集33例扩张术患者的51枚扩张囊内、外的液体标本，进行常规细菌培养和药敏试验，并将其结果进行统计学处理，采用Y0检验和Kappa检验。结果扩张囊内、外的细菌检出率分别为3．92％（2枚）、17．65％（9枚），囊外阳性率显著高于囊内（P〈0．05），囊内、外细菌的检出结果一致性较差（Kappa〈0．4）。细菌种类为金黄色葡萄球菌和凝固酶阴性葡萄球菌，对庆大霉素、去甲万古霉素、环丙沙星均敏感。33例患者（51枚）中，有10例患者（10枚）检出细菌，仅有1例患者（1枚）出现临床感染症状，致病菌为金黄色葡萄球菌。结论严格消毒后进行穿刺注水导致感染的几率很低，而感染后的细菌培养和药敏试验是治疗的关键。