改良V-W成形矫正内眦赘皮

目的寻求一种简单有效的内眦赘皮矫治方法.方法采用改良V-W成形,即赘皮皮肤的V-W推进改形,加上新内眦点与鼻根外侧的筋膜或骨膜固定、松解皮下粘连、修整错构的眼轮匝肌等,全面治疗内眦赘皮.大部分病例与重睑术同期进行.结果1999年至今矫治明显的内眦赘皮67例,术后效果满意.术后1个月有5例出现内眦部切口增生发红,3-4月逐渐软化消退.所有病例随访1个月到1年,均获得满意疗效.结论改良V-W成形手术切口设计简单明了,术后内眦形态良好,不易复发,瘢痕不显,是矫正内眦赘皮较理想的方法.     

白细胞介素-4和γ-干扰素基因联合治疗胶质瘤

目的 探讨逆转录病毒载体介导的白细胞介素(IL)-4和γ-干扰素(IFN)基因联合治疗胶质瘤的作用。方法构建携带IL-4或IFN-γ基因的逆转录病毒载体，将其导入逆转录病毒包装细胞；将携带IL-4和IFN-γ基因的逆转录病毒包装细胞分别或联合注射到脑内荷瘤大鼠的肿瘤组织中，观察其治疗作用。结果成功获得携带治疗基因的逆转录病毒包装细胞PA317IFN-γ和PA317IL-4，所产病毒滴度分别为2x10^6cfu／ml和2．5x10^6cfu／ml。包装细胞瘤内注射能够诱导大鼠产生抗肿瘤免疫反应，杀伤肿瘤细胞，联合应用具有协同治疗作用。结论携带IL-4或IFN-γ基因的逆转录病毒包装细胞瘤内注射是一种有效的胶质瘤基因治疗方法，两者联合应用具有协同治疗作用。     

原发性肝癌合并糖尿病的介入治疗

目的　研究原发性肝癌合并糖尿病的介入治疗方法。方法　临床明确诊断的 3 4例肝癌合并糖尿病患者在介入治疗前后通过口服降糖药或注射胰岛素将血糖、尿糖控制在安全范围内 ,并对隐性感染或感染源进行治疗 ,然后再行肝动脉化疗灌注栓塞术。结果　 3 4例患者介入治疗前血糖均降到 9.0mmol/L以下 ,尿糖控制在 -～ ++,术后均未发生严重并发症 ,仅有 2例出现慢性胆囊炎急性发作 ,经抗炎、利胆治疗后安全出院。结论　肝癌合并糖尿病患者在血糖、尿糖降到一定的范围内 ,消除潜在的感染 ,行肝动脉化疗栓塞治疗是安全可行的     

反相高压液相色谱法测定血清中部分氨基酸

目的：建立测定血清中精氨酸含量的方法。方法：反相高压液相色谱法。ＨｙｐｅｒｓｉｌＢＤＳ柱，邻苯二甲醛柱前衍生化、萤光检测法检测。结果：精氨酸线性范围为３８３μｍｏｌ／Ｌ～４７８９μｍｏｌ／Ｌ，８种氨基酸的最低检测限为２μｍｏｌ／Ｌ，日内变异系数为２７５％～１１９３％，日间变异系数为７０５％～１２３９％，５种氨基酸的回收率为９６３３％～１００７８％。正常人血清精氨酸水平为９２７７±１７９０μｍｏｌ／Ｌ，糖尿病患者血清精氨酸水平则为７８６１±３３０３μｍｏｌ／Ｌ（Ｐ＞００５）。结论：本方法简单易行，方法学实验结果满意，适用于临床测定相关氨基酸的需要     

脑肿瘤中p53基因突变的分析

检测２５例脑肿瘤中抑制癌基因Ｐ５３突变情况。突用复合ＰＣＲ，聚合酶链反应－＝单链构象多态性分析法，统计学处理采用Ｘ＾２检验。结果：Ｐ５３基因总的突变率为４４．０％，其中第５外显子突变率为２８．０％，第６外显子突变率１２．０％，第７外显子突变率为１２．０％，第８外显子突变率１６．０％；病理分级Ⅱ级及其以上突变为７２．７％，     

石棉相关肿瘤p53基因突变的免疫组化及PCR—SSCP研究

为了分析石棉相关肿瘤ｐ５３基因的突变特点，对石蜡包埋的石棉相关肿瘤ｐ５３基因突变体蛋白的表达进行了免疫组化观察，提取染色体ＤＮＡ，对ｐ５３基因的第５、７、８外显子进行ＰＣＲ－ＳＳＣＰ及测序分析。免疫组化观察发现：在分析的１０例病例中有５例阳性。ＰＣＲ－ＳＳＣＰ分析发现７例（８处）发生突变，其中４处集中在第８外显子上。５例腺癌中有４例发生ｐ５３基因突变。测序发现热点区突变。提示：石棉相关肿瘤ｐ５３基因突变率高。     

雾化吸入对新生儿肺炎患儿血氧饱和度的影响

采用动脉血氧饱和度仪对５２例新生儿肺炎患儿，在雾化吸入治疗过程中的动脉血氧饱和度（ＳａＯ２）进行了监测，对比了吸氧与否对ＳａＯ２改变的影响。结查表明：与基础值比较，雾化吸入时患儿的ＳａＯ２显著下降（Ｐ＜０．０１），而雾化吸入同时吸氧者ＳａＯ２无明显变化（Ｐ＞０．０５），但两者比较具有显著性差异（Ｐ＜０．０１）。结果提示雾化吸入可使新生儿肺炎患儿ＳａＯ２下降；同时吸氧，对防止ＳａＯ２的下降有一定作用。     

甲基强的松龙冲击疗法治疗重型脱髓鞘脑病患者的护理

采用大剂量甲基强的松龙治疗脱髓鞘脑病患者１５例。在治疗过程中，积极预防应激性溃疡，加强呼吸道及人工冬眠护理。经５次治疗，１４例症状明显改善。     

Region-specific growth properties and trophic requirements of brain- and spinal cord-derived rat embryonic neural precursor cells

To determine whether neural precursor cells have region-specific growth properties, we compared the proliferation, mitogenicity, and differentiation of these cells isolated from the embryonic day 16 rat forebrain and spinal cord. Neural precursor cells isolated from both regions were cultured in growth medium supplemented with epidermal growth factor, basic fibroblast growth factor, or epidermal growth factor+basic fibroblast growth factor. Under all three conditions, both neural precursor cell populations proliferated for multiple passages. While spinal cord-derived neural precursor cells proliferated moderately faster in epidermal growth factor-enriched growth medium, brain-derived cells proliferated much faster in basic fibroblast growth factor-enriched growth medium. When exposed to both epidermal growth factor and basic fibroblast growth factor, the two neural precursor cell populations expanded and proliferated more rapidly than when exposed to a single factor, with brain-derived neural precursor cells expanding significantly faster than spinal cord-derived ones (P<0.0001). Differentiation studies showed that both neural precursor cell populations were multi-potent giving rise to neurons, astrocytes, and oligodendrocytes. However, neuronal differentiation from brain-derived neural precursor cells was greater than spinal cord-derived ones (11.95+/-5.00% vs 1.92+/-1.13%; passage 2). Further, the two neural precursor cell populations differentiated into a similar percentage of oligodendrocytes (brain: 8.66+/-5.85%; spinal cord: 7.69+/-3.91%; passage 2). Immunofluorescence and Western blot studies showed that neural precursor cells derived from both regions expressed receptors for basic fibroblast growth factor and epidermal growth factor. However, brain-derived neural precursor cells expressed higher levels of the two receptors than spinal cord-derived ones in growth medium containing epidermal growth factor+basic fibroblast growth factor. Thus, our results showed that neural precursor cells isolated from the two regions of the CNS have distinct properties and growth requirements. Identifying phenotypic differences between these neural precursor cell populations and their growth requirements should provide new insights into the development of cell therapies for region-specific neurological degenerative diseases.