前列腺素E1对大鼠卵泡壁发育和分化影响的形态学观察

本文应用组织学方法和电镜对PGE1引起未成熟大鼠卵泡颗粒细胞和卵泡膜外层细胞的形态学变化作了研究。结果表明:外源性PGE1刺激了颗粒细胞的发育和黄体化,同时也促进了卵泡膜外层细胞的发育和从成纤维细胞向平滑肌细胞分化。文中对有关PGE1在排卵过程中的作用机制等问题进行了讨论。     

HLA-DPA1 promoter haplotypes are differently distributed in southern Chinese ethnic groups

DPA1 gene is one of the human leukocyte antigen (HLA) class II genes and its promoter is highly polymorphic. From comparative studies among five southern Chinese populations, Jing, Li, Bai, Lahu, and Meizhou Han, we describe their single-nucleotide polymorphism (SNP)/haplotype frequency data of HLA-DPA1 gene promoter in this study. Within the 760-bp promoter region, we have identified 21 SNPs and nine possible haplotypes. Pair-wise comparisons show similar frequencies distribution of the HLA-DPA1 promoter haplotypes among Jing, Li, and Bai, whereas all pair-wise comparisons involved with Lahu or Meizhou Han and other ethnic groups show remarkable difference. The differences in frequencies of HLA-DPA1 promoter alleles may reveal different ethnic origins and demographic histories of the five populations. Our study may help distinguishing each of these populations by sequence variations of HLA-DPA1 promoter, which may be served as functional molecular markers for clinical and immunological studies involving the DPA1 locus.     

Cloning and characterization of a novel gene which encodes a protein interacting with the mitosis-associated kinase-like protein NTKL

NTKL is an evolutionarily conserved kinase-like protein. The cell-cycle-dependent centrosomal localization of NTKL suggested that it was involved in centrosome-related cellular function. The mouse NTKL protein is highly homologous with human NTKL. A novel mouse protein was identified as an NTKL-binding protein (NTKL-BP1) by yeast two-hybrid screening, and the full-length cDNA was amplified based on the result of a sequence data analysis cloning strategy. The full-length cDNA sequence of the NTKL-BP1 gene consists of 2,537 bp, which encode 368 amino acids. A database search revealed that homologues of NTKL-BP1 exist in different organisms, including Arabidopsis thaliana, Drosophila melanogaster, Plasmodium falciparum, Geobacter metallireducens, Anopheles gambiae and human. It suggests that NTKL-BP1 is an evolutionarily conserved protein. The expression of NTKL-BP1 was observed in multiple normal mouse tissues. The interaction of the two proteins was confirmed by co-immunoprecipitation. Moreover, immunofluorescent staining indicated that NTKL and NTKL-BP1 were all localized in the cytoplasm.     

NMDA receptor mediated dendritic plasticity in cortical cultures after oxygen-glucose deprivation

Dendrites and spines undergo dynamic changes in physiological and pathological conditions. Dendritic outgrowth has been observed in surviving neurons months after ischemia, which is associated with the functional compensation. It remains unclear how dendrites in surviving neurons are altered shortly after ischemia, which might reveal the mechanisms underlying neuronal survival. Using primary cortical cultures, we monitored the dendritic changes in individual neurons after oxygen-glucose deprivation (OGD). Two to four hours of OGD induced approximately 30–50% cell death in 24 h. However, the total dendritic length in surviving neurons was significantly increased after OGD with a peak at 6 h after re-oxygenation. The increase of dendritic length after OGD was mainly due to the sprouting rather than the extension of the dendrites. The dendritic outgrowth after 2 h of OGD was greater than that after 4 h of OGD. Application of NMDA receptor blocker MK-801 abolished OGD-induced dendritic outgrowth, whereas application of AMPA receptor antagonist CNQX had no significant effects. These results demonstrate a NMDA receptor-dependent dendritic plasticity shortly after OGD, which provides insights into the early response of surviving neurons after ischemia.     

LHRH药代动力学试验

目的:研究黄体激素释放激素(Luteinizinghormone releasinghormone,LHRH)在大鼠体内的药动学规律。方法:用氯胺T法将125I标记到LHRH分子上(放化纯度96.2%),14只SD大鼠随机分为大剂量、小剂量组,每组7只,分别肌肉注射125I LHRH(小剂量组0.5mg.kg,大剂量组1.00mg.kg),给药后在21个不同时间点逐一取各鼠尾动脉血做放射性测定。结果:大鼠试验结果显示,大剂量组t1/2平均为67.83±20.84h;小剂量组半衰期平均为64.68±22.90h。LHRH的药—时曲线符合二室模型,大剂量LHRH和小剂量LHRH的主要药动学指标差别不大。结论:LHRH在大鼠体内的消除模式为二室模型,为一级动力学消除。     

微波辐射免疫比浊法加速测定免疫球蛋白

本文应用微波辐射免疫比浊法加速测定免疫球蛋白(IgG、IgA、IgM).结果表明:用本法测定的线性范围较宽:IgG从3.24～51.92/L;IgA从0.49～7.92g/L;IgM从0.36～5.75/L.批内、批间精密度(CV):IgG从44～7.4%;IgA从4.1～9.5%;IgM从4.0～8.8%.本法与传统免疫比浊法和进口试剂自动免疫比浊法(Monarch 2000)测定比较,其结果相关良好,r从0.950至0.996,P>0.05.本法的特点是操作简便、快速,可明显缩短抗原和抗体反应达到终点的时间.     

高血压鼠动脉壁去甲肾上腺素含量及形态变化研究

取不同年龄组自发性高血压鼠的胸主动脉，颈劝脉和基底动脉，用高效液相色谱－电化学检测仪测定其去甲肾上腺素含量，并取脑软膜血管作超微结构观察，结果显示：各组高血压鼠动有脉壁去甲肾上腺素含量均下降，颈内动脉更明显；脑血管内弹性膜部分断裂，平滑肌细胞有空不包变性等。     

近场射频辐射条件下大白鼠共振吸收频率的研究

在Ｅ极化、辐射电平１ｍｗ／ｃｍ２条件下，在４０．６８ＭＨｚ～４ＧＨｚ频段内６个频率点上，利用红外热图术观察了６０只Ｗｉｓｔａｒ成年大白鼠，连续辐射１ｈ后，体表及各部位体表温度变化。结果表明，体表温度与肛温变化有很好的一致性，并在１．５ＧＨｚ有最大值。它表明在该频率下，实验鼠吸收射频能量最大，表现出明显的谐振吸引特性。     

鲎试验测定复方氨基酸(15)双肽(2)注射液中的细菌内毒素

目的:检测复方氨基酸(15)双肽(2)注射液中的细菌内毒素。方法:供试品干扰试验后,采用凝胶鲎试验对样品中的细菌内毒素进行检测。结果:三批复方氨基酸(15)双肽(2)注射液稀释至28倍时对细菌内毒素的检测无干扰作用,所测样品的细菌内毒素含量均小于7 EU.ml-1。结论:所建立方法检查复方氨基酸(15)双肽(2)注射液中的细菌内毒素可行。