小型猪急性心肌梗死后心力衰竭模型的构建

目的研究开放拉长塑料细管法（OPS）对HBV转基因小鼠（P21^HBxPHBx）和白内障小鼠（cataract mice,cM）胚胎冷冻效果。方法采用乙二醇作为冷冻保护剂,以昆明小鼠为对照,用OPS法对CM及P21^HBxPHBx两种小鼠3．5d的胚胎进行玻璃化冷冻,并比较KM、CM及P21^HBxPHBx获得胚胎数,解冻后胚胎的复苏率及发育率。结果KM、CM及P21^HBxPHBx小鼠平均获得胚胎数分别为31．12、19．32、9．14枚;解冻后的复苏率分别为95．6％、79-3％、80．3％;发育率分别为94．7％、73．8％、56．6％。表明HBV转基因小鼠的获得胚胎数明显低于昆明小鼠,解冻后三种小鼠的胚胎复苏率之间无显著差别,而解冻后发育率,P21^HBxPHBx小鼠要明显低于KM小鼠。结论转基因小鼠超排数及解冻后发育率低于昆明小鼠,而白内障模型小鼠和昆明小鼠比较不显著。     

Clinical Research on Comprehensive Treatment of Senile Vascular Dementia

Objective:To observe the curative effect of combined Chinese medicine,acupuncture and rehabilitation therapy on vascular dementia(VD),and to compare the Western medicine piracetam.Methods:Total 134 VD patients screened in reference to the standard for VD diagnosis in DSM-IV were randomly divided into a Chinese medicine plus rehabilitation group(32 cases),a Chinese medicine plus acupuncture group(33 cases),a Chinese medicine and acupuncture plus rehabilitation group(37 cases) and a Western medicine piracetam...     

空气消毒方法应用研究进展

本文概述了国内外室内空气消毒方法研究应用进展,主要包括空气消毒方法的种类、各种空气消毒方法。     

病毒性脑膜炎和病毒性脑炎患者脑脊液中一氧化氮的变化及意义

目的 探讨病毒性脑膜炎和病毒性脑炎(即病脑)患者脑脊液中一氧化氮(NO)含量改变的临床意义。方法 应用相应试剂盒对27例病脑患者和15例非感染者(对照组)脑脊液中的NO进行测定。结果 病脑患者脑脊液中治疗前NO含量与治疗后及对照组比较明显增高(P＜0．01)。结论 提示NO确实参与了病脑的病理过程，NO含量测定有助于病脑的诊断及了解病情变化。     

高氧液对单肺通气低氧血症和肺内分流的影响

目的探讨双腔支气管内麻醉时,输入高氧液对单肺通气时低氧血症和肺内分流(Qs/Qt)的影响。方法选择食管中段癌根治手术20例,随机分为两组,N组(对照组)10例单肺通气时输入平衡液;O组(高氧液组)10例单肺通气时输入高氧平衡液。分别于麻醉前、双肺通气(DLV)30min、单肺通气(OLV)30min抽取动脉血和混合静脉血做血气分析,并计算Qs/Qt等。结果OLV时O组动脉血氧饱和度(SaO2)显著高于N组(P<0.01),O组动脉血氧含量(CaO2)显著高于N组(P<0.05)。OLV时,Qs/Qt较DLV时显著增加(N组P<0.01;O组P<0.05),但两组间比较差异无显著性。结论静脉输注高氧液可以明显升高SaO2和CaO2,高氧液对Qs/Qt的影响不明显。单肺通气时输注高氧液可能对低氧血症有利。     

长春西汀治疗血管性痴呆的临床疗效观察

目的:探讨长春西汀对血管性痴呆(VD)疗效和作用机制。方法:96例VD患者随机分成长春西汀治疗组和胞二磷胆碱对照组,分别在治疗第10、21d进行简易精神状态评分(MMSE)和长谷川评分(1DS),同时测定与记忆有关的乙酰胆碱酯酶(AchE)的活性。结果:长春西汀治疗组患者第21d的MMSE和IDS评分均高于治疗前和一般治疗组(P＜0．01),且长春西汀治疗组VD患者的乙酰胆碱酯酶(AchE)活性降低和血浆乙酰胆(Ach)含量升高(P＜0．05)。结论:长春西汀治疗VD疗效肯定,其机制可能与胆碱能神经系统功能增强有关。     

综合评价医院工作的指标加权百分位次累加法

本文提出了综合评价医院工作的一种方法——指标加权百分位次累加法。本方法分五个步骤:1.建立评价指标体系;2.制定评价标准值;3.对指标值进行标准化;4.根据指标重要程度赋予相应权重;5.进行综合评价。并通过实际资料对本方法进行了验证。     

组织芯片技术与人工智能神经网络在大肠肿瘤诊断中的应用

目的：构建组织原位检测指标预测诊断大肠肿瘤的人工智能神经网络（ANN）模型,探讨组织芯片技术与ANN结合应用的可行性。方法：应用组织芯片技术检测ST13等8种组织原位检测指标在大肠肿瘤演进过程各阶段的表达,同时采用ANN构建相应的诊断模型。结果：采用Matlab6．5软件中提供的Kruskal-wal-lis H秩和检验函数,对这8种指标在正常大肠组织、大肠腺瘤和大肠癌组织中的阳性表达差异进行统计学检验,其中ST13、Bcl-2、Survivin和HSFlm RNA的表达,差异有统计学意义,P〈0．01;将8种指标随机组合,分别构建相应的ANN诊断模型,评价其各自的诊断效率,发现ST13、Bcl-2、Survivin与HSFl mRNA组合的ANN-BP模型预测准确率最高,其对正常大肠组织、大肠腺瘤和大肠癌训练集的预测准确率分别高达92.895％、94.163％和92.013％,对该ANN-BP网络诊断模型的盲法测试结果也分别高达85.714％、79.412％和72％。结论：组织芯片技术与ANN相结合,可以大大提高组织原位检测指标对大肠肿瘤的预测诊断效率。     

The frequency of assessment tools in arthroscopic training: a systematic review

BackgroundMultiple assessment tools are used in arthroscopic training and play an important role in feedback. However, it is not fully recognized as to the standard way to apply these tools. Our study aimed to investigate the use of assessment tools in arthroscopic training and determine whether there is an optimal way to apply various assessment tools in arthroscopic training.MethodsA search was performed using PubMed, Embase and Cochrane Library electronic databases for articles published in English from January 2000 to July 2021. Eligible for inclusion were primary research articles related to using assessment tools for the evaluation of arthroscopic skills and training environments. Studies that focussed only on therapeutic cases, did not report outcome measures of technical skills, or did not mention arthroscopic skills training were excluded.ResultsA total of 28 studies were included for review. Multiple assessment tools were used in arthroscopic training. The most common objective metric was completion time, reported in 21 studies. Technical parameters based on simulator or external equipment, such as instrument path length, hand movement, visual parameters and injury, were also widely used. Subjective assessment tools included checklists and global rating scales (GRS). Among these, the most commonly used GRS was the Arthroscopic Surgical Skill Evaluation Tool (ASSET). Most of the studies combined objective metrics and subjective assessment scales in the evaluation of arthroscopic skill trainingConclusionsOverall, both subjective and objective assessment tools can be used as feedback for basic arthroscopic skill training, but there are still differences in the frequency of application in different contexts. Despite this, combined use of subjective and objective assessment tools can be applied to more situations and skills and can be the optimal way for assessment.Level of EvidenceLevel III, systematic review of level I to III studies.

Key messages

• Both subjective and objective assessment tools can be used as feedback for basic arthroscopic skill training.
• Combined use of subjective and objective assessment tools can be applied to more situations and skills and can be the optimal way for assessment.