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31.
Impact of the International Continence Society (ICS) report on the standardisation of terminology in nocturia on the quality of reports on nocturia and nocturnal polyuria: a systematic review 下载免费PDF全文
32.
Sandeep K. Mallipattu Sylvia J. Horne Vivette D’Agati Goutham Narla Ruijie Liu Michael A. Frohman Kathleen Dickman Edward Y. Chen Avi Ma’ayan Agnieszka B. Bialkowska Amr M. Ghaleb Mandayam O. Nandan Mukesh K. Jain Ilse Daehn Peter Y. Chuang Vincent W. Yang John C. He 《The Journal of clinical investigation》2015,125(3):1347-1361
33.
Stamatis Adamopoulos Jean‐Paul Schmid Paul Dendale Daniel Poerschke Dominique Hansen Athanasios Dritsas Alexandros Kouloubinis Toon Alders Aggeliki Gkouziouta Ilse Reyckers Vasiliki Vartela Nikos Plessas Costas Doulaptsis Hugo Saner Ioannis D. Laoutaris 《European journal of heart failure》2014,16(5):574-582
34.
Differential role of NK cells against Candida albicans infection in immunocompetent or immunocompromised mice 下载免费PDF全文
Jessica Quintin Jessica Voigt Robbert van der Voort Ilse D. Jacobsen Ineke Verschueren Bernhard Hube Evangelos J. Giamarellos‐Bourboulis Jos W. M. van der Meer Leo A. B. Joosten Oliver Kurzai Mihai G. Netea 《European journal of immunology》2014,44(8):2405-2414
Little is known regarding the role of NK cells during primary and secondary disseminated Candida albicans infection. We assessed the role of NK cells for host defense against candidiasis in immunocompetent, as well as immunodeficient, hosts. Surprisingly, depletion of NK cells in immunocompetent WT mice did not increase susceptibility to systemic candidiasis, suggesting that NK cells are redundant for antifungal defense in otherwise immunocompetent hosts. NK‐cell‐depleted mice were found to be protected as a consequence of attenuation of systemic inflammation. In contrast, the absence of NK cells in T/B/NK‐cell‐deficient NSG (NOD SCID gamma) mice led to an increased susceptibility to both primary and secondary systemic C. albicans infections compared with T/B‐cell‐deficient SCID mice. In conclusion, this study demonstrates that NK cells are an essential and nonredundant component of anti‐C. albicans host defense in immunosuppressed hosts with defective T/B‐lymphocyte immunity, while contributing to hyperinflammation in immunocompetent hosts. The discovery of the importance of NK cells in hosts with severe defects of adaptive immunity might have important consequences for the design of adjunctive immunotherapeutic approaches in systemic C. albicans infections targeting NK‐cell function. 相似文献
35.
Pancreatic ductal adenocarcinoma (PDAC) has long been considered to arise from pancreatic ducts on the basis of its morphology, the occurrence of dysplasia in putative preneoplastic ductal lesions, and the absence of acinar dysplasia in the pancreas of patients with PDAC. However, evidence gathered through both in vitro studies and--more importantly--genetic mouse models of PDAC shows that ductal-type tumours can arise from acinar cells. These findings raise new important questions related to PDAC pathophysiology and call for in-depth studies of acinar cell differentiation in order to better understand PDAC biology. The authors review these issues and discuss how the novel findings should impact on future work aiming at early diagnosis and improved outcome of patients with PDAC. 相似文献
36.
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38.
Herrmann H Cerny-Reiterer S Gleixner KV Blatt K Herndlhofer S Rabitsch W Jäger E Mitterbauer-Hohendanner G Streubel B Selzer E Schwarzinger I Sperr WR Valent P 《Haematologica》2012,97(2):219-226
Background
CD33 is a well-known stem cell target in acute myeloid leukemia. So far, however, little is known about expression of CD33 on leukemic stem cells in chronic leukemias.Design and Methods
We analyzed expression of CD33 in leukemic progenitors in chronic myeloid leukemia by multi-color flow cytometry and quantitative polymerase chain reaction. In addition, the effects of a CD33-targeting drug, gemtuzumab/ozogamicin, were examined.Results
As assessed by flow cytometry, stem cell-enriched CD34+/CD38−/CD123+ leukemic cells expressed significantly higher levels of CD33 compared to normal CD34+/CD38− stem cells. Moreover, highly enriched leukemic CD34+/CD38− cells (>98% purity) displayed higher levels of CD33 mRNA. In chronic phase patients, CD33 was found to be expressed invariably on most or all stem cells, whereas in accelerated or blast phase of the disease, the levels of CD33 on stem cells varied from donor to donor. The MDR1 antigen, supposedly involved in resistance against ozogamicin, was not detectable on leukemic CD34+/CD38− cells. Correspondingly, gemtuzumab/ozogamicin produced growth inhibition in leukemic progenitor cells in all patients tested. The effects of gemtuzumab/ozogamicin were dose-dependent, occurred at low concentrations, and were accompanied by apoptosis in suspension culture. Moreover, the drug was found to inhibit growth of leukemic cells in a colony assay and long-term culture-initiating cell assay. Finally, gemtuzumab/ozogamicin was found to synergize with nilotinib and bosutinib in inducing growth inhibition in leukemic cells.Conclusions
CD33 is expressed abundantly on immature CD34+/CD38− stem cells and may serve as a stem cell target in chronic myeloid leukemia. 相似文献39.
Yasemin Erbas Eva Ceulemans Johanna Boonen Ilse Noens Peter Kuppens 《Research in autism spectrum disorders》2013,7(10):1221-1227
Autism spectrum disorder (ASD) is commonly associated with reduced ability to recognize emotions in others. It is less clear however, whether ASD is also associated with impaired knowledge of one's own emotions. In the current study we present a first examination of how much knowledge individuals with ASD have about their emotions by investigating their ability to differentiate between emotions. Across two lab tasks that measured to what extent and how people differentiate between their own feeling states and semantic emotion terms, results showed that ASD individuals differentiated less than typically developing individuals. Yet, both groups of participants similarly categorized emotions according to previously established theoretical categories. These findings indicate that while both give similar meaning to emotions, individuals with ASD make less subtle distinctions between emotions. With low levels of emotion differentiation being linked to reduced well-being, these findings may help to better understand the high prevalence of internalizing problems associated with ASD. 相似文献
40.
Siemerink MJ Klaassen I Vogels IM Griffioen AW Van Noorden CJ Schlingemann RO 《Angiogenesis》2012,15(1):151-163
The functional shift of quiescent endothelial cells into tip cells that migrate and stalk cells that proliferate is a key
event during sprouting angiogenesis. We previously showed that the sialomucin CD34 is expressed in a small subset of cultured
endothelial cells and that these cells extend filopodia: a hallmark of tip cells in vivo. In the present study, we characterized
endothelial cells expressing CD34 in endothelial monolayers in vitro. We found that CD34-positive human umbilical vein endothelial
cells show low proliferation activity and increased mRNA expression of all known tip cell markers, as compared to CD34-negative
cells. Genome-wide mRNA profiling analysis of CD34-positive endothelial cells demonstrated enrichment for biological functions
related to angiogenesis and migration, whereas CD34-negative cells were enriched for functions related to proliferation. In
addition, we found an increase or decrease of CD34-positive cells in vitro upon exposure to stimuli that enhance or limit
the number of tip cells in vivo, respectively. Our findings suggest cells with virtually all known properties of tip cells
are present in vascular endothelial cell cultures and that they can be isolated based on expression of CD34. This novel strategy
may open alternative avenues for future studies of molecular processes and functions in tip cells in angiogenesis. 相似文献