中药穴位敷贴治疗儿童哮喘基础和临床研究现状

从基础研究和临床研究2个方面,包括中医理论研究、实验研究、总体疗效、改善症状、调理体质、疗程时间等,总结了中药穴位敷贴治疗儿童哮喘的研究现状。指出今后研究可设计大样本以便随机对照临床试验研究,同时可在穴位敷贴对儿童中医体质的改善方面开展,以期探讨穴位敷贴的中医作用机制。     

呼吸减痛法配合一对一陪伴分娩临床研究

目的探讨呼吸减痛法配合一对一陪伴分娩的临床效果。方法选取在孕期接受呼吸减痛法培训的初产妇50例做为观察组,选取未接受呼吸减痛法培训的初产妇50例作为对照组,临产时观察组由一名专职助产士有针对性地进行呼吸减痛法的指导,对照组不做呼吸减痛法的指导,仅采用传统的方法进行待产。两组孕妇均按常规一对一陪伴分娩,按正常方法观察产程,收集相关资料后进行统计学分析。结果观察组中出现Ⅰ级与Ⅱ级分娩疼痛的产妇较对照组增加,而Ⅲ级分娩疼痛者明显少于对照组;第一产程、第二产程和总产程时间明显短于对照组,产后出血率、新生儿窒息率明显少于对照组,差异均有统计学意义(P<0.05)。结论呼吸减痛分娩法是一种安全、有效的减痛分娩方法,可以缩短总产程,减少产后出血及新生儿窒息的发生。     

小儿肺炎支原体肺炎合并心肌损害的临床分析

目的 探讨肺炎支原体肺炎（MPP）合并心肌损害的临床特点.方法 选择我院2012年1月至2013年6月确诊为MPP的患儿共478例,按年龄进行分组,分别比较不同年龄组间心肌损害的发生率.结果 0～1岁患儿中合并心肌损害的有75例,占0～1岁患儿的28.4％;2～3岁患儿中有27例,占20.5％;4～5岁患儿中有9例,占16.4％;大于6岁患儿中有4人,占14.8％.MMP患儿合并心肌损害的发生率与年龄呈负相关（r=-0.722,P＜0.001）.结论 MPP在婴幼儿中并不少见,且更易合并心肌损害.     

Vitamin D and the Risks of Depression and Anxiety: An Observational Analysis and Genome-Wide Environment Interaction Study

Previous studies have suggested that vitamin D (VD) was associated with psychiatric diseases, but efforts to elucidate the functional relevance of VD with depression and anxiety from genetic perspective have been limited. Based on the UK Biobank cohort, we first calculated polygenic risk score (PRS) for VD from genome-wide association study (GWAS) data of VD. Linear and logistic regression analysis were conducted to evaluate the associations of VD traits with depression and anxiety traits, respectively. Then, using individual genotype and phenotype data from the UK Biobank, genome-wide environment interaction studies (GWEIS) were performed to identify the potential effects of gene × VD interactions on the risks of depression and anxiety traits. In the UK Biobank cohort, we observed significant associations of blood VD level with depression and anxiety traits, as well as significant associations of VD PRS and depression and anxiety traits. GWEIS identified multiple candidate loci, such as rs114086183 (p = 4.11 × 10⁻⁸, LRRTM4) for self-reported depression status and rs149760119 (p = 3.88 × 10⁻⁸, GNB5) for self-reported anxiety status. Our study results suggested that VD was negatively associated with depression and anxiety. GWEIS identified multiple candidate genes interacting with VD, providing novel clues for understanding the biological mechanism potential associations between VD and psychiatric disorders.     

芒果苷对胰岛素抵抗HepG2细胞糖脂代谢的影响

目的:分析芒果苷(MGF)对胰岛素抵抗HepG2细胞(IR-HepG2细胞)糖脂代谢的影响,并探讨潜在机制.方法:以人肝癌HepG2细胞为对象,以1 mmol/L棕榈酸+2 mmol/L油酸联合培养建立IR-HepG2细胞模型.以盐酸二甲双胍为阳性对照,分别检测低、中、高浓度MGF(125、250、500μmol/L)...     

Protective effect of resveratrol on arsenic trioxide-induced nephrotoxicity in rats

BACKGROUD/OBEJECTIVES

Arsenic, which causes human carcinogenicity, is ubiquitous in the environment. This study was designed to evaluate modulation of arsenic induced cancer by resveratrol, a phytoalexin found in vegetal dietary sources that has antioxidant and chemopreventive properties, in arsenic trioxide (As₂O₃)-induced Male Wistar rats.

MATERIALS/METHODS

Adult rats received 3 mg/kg As₂O₃ (intravenous injection, iv.) on alternate days for 4 days. Resveratrol (8 mg/kg) was administered (iv.) 1 h before As₂O₃ treatment. The plasma and homogenization enzymes associated with oxidative stress of rat kidneys were measured, the kidneys were examined histologically and trace element contents were assessed.

RESULTS

Rats treated with As₂O₃ had significantly higher oxidative stress and kidney arsenic accumulation; however, pretreatment with resveratrol reversed these changes. In addition, prior to treatment with resveratrol resulted in lower blood urea nitrogen, creatinine and insignificant renal tubular epithelial cell necrosis. Furthermore, the presence of resveratrol preserved the selenium content (0.805 ± 0.059 µg/g) of kidneys in rats treated with As₂O₃. However, resveratrol had no effect on zinc level in the kidney relative to As₂O₃-treated groups.

CONCLUSIONS

Our data show that supplementation with resveratrol alleviated nephrotoxicity by improving antioxidant capacity and arsenic efflux. These findings suggest that resveratrol has the potential to protect against kidney damage in populations exposed to arsenic.     

活血化瘀法治疗肢体闭塞性动脉硬化症136例效果探讨

目的探讨活血化瘀法治疗肢体闭塞性动脉硬化症临床应用效果。方法对照组接受临床常规西医治疗,研究组在西医常规治疗基础上加用中医活血化瘀法,记录两组肢体闭塞性动脉硬化症患者临床疗效。结果两组肢体闭塞性动脉硬化症患者均顺利完成相应治疗,研究组临床治疗总有效率(86.03%)显著高于对照组(61.76%),数据对比P0.05。结论肢体闭塞性动脉硬化症患者在常规西医治疗基础上加用中医活血化瘀法可显著提高其临床疗效,有利于保障患者生活质量及身心健康。     

DNA甲基转移酶1与甲基-CpG-结合蛋白2异常表达在宫颈病变中的作用及相互关系

目的 探讨DNA甲基转移酶1(DNMTl)和甲基-CPG-结合蛋白2(MecP2)与宫颈癌发生发展的关系及其在宫颈癌变中的相互作用。方法 选择经病理学确诊的宫颈鳞状细胞癌(SCC)患者74例、低度宫颈上皮内瘤样变(CIN I)患者52例、高度宫颈上皮内瘤样变(CINⅡ一Ⅲ)患者60例和宫颈炎(CI)患者58例为研究对象。在收集全部对象人口学特征、人乳头瘤病毒感染、生殖因素等相关资料同时,检测DNMTl和MeCP2蛋白(Western blot法)和mRNA(real-time PCR法)的相对表达量。采用SPSS 17.0分析软件,计算相关资料的t检验、X²检验、因素与疾病之间关联强度指标(0R值及其95%CI)及其交互作用。结果 随着宫颈病变的加重,DNMTl和MeCP2蛋白表达水平逐渐增高(H=94.33,PF=21.580,P<0.001),DNMTl和MeCP2 mRNA的表达水平亦逐渐增高(F=4.758,P=0.003;F=7。804,Pr=0.287,P<0.001)和mRNA(r=0.179,P=0.005)表达均存在正相关关系。交互作用分析显示,DNMTl与MeCP2蛋白和mRNA高表达在SCC组和CINl/-Ⅲ组存在正相加交互作用。结论 DNMTl及MeCP2高表达可增加宫颈癌和癌前病变发生的风险,两者在C1N II-Ⅲ组和SCC组存在正相加交互作用。     

先天性心脏病合并肺动脉高压患者的麻醉处理

肺动脉高压常见于肺血流增多的先天性心脏缺损,其发生率和严重程度与缺损的性质有关。总体而言,肺动脉高压是一类预后很差的综合征,尽管药物治疗有所发展,但至今仍以手术治疗为主。此类手术宜尽早在婴幼儿时期施行,以免病情随年龄增长而恶化。手术及麻醉过程中的很多因素可使肺血管阻力增高,如缺氧、手术刺激、炎性介质等,对于先天性心脏病合并肺动脉高压的患者选择合适的麻醉方式和药物维持循环稳定,防止肺循环阻力进一步增高至关重要。现就国内对先心病合并肺动脉高压患者的麻醉管理的研究进展作一综述。     

氢杨梅素改善高脂饮食诱导的肥胖小鼠肝脏脂肪沉积及机制（英文）

有文献报道, SIRT1-AMPK信号通路可能在DHM改善肝脏细胞甘油三酯蓄积、胰岛素抵抗等作用中发挥作用。为此,本课题拟进一步观察DHM对高脂饮食诱导的肥胖小鼠肝脏脂肪沉积的影响,并探讨其可能机制。C57BL/6J小鼠采用普通饲料和高脂饲料喂养,同时分别用或不用低剂量(125 mg/kg)或高剂量(250 mg/kg)的DHM处理16周。实验期间,每两周检测体重一次。16周后,眼眶静脉取血并处死动物,同时取肩胛下、附睾与腹股沟的脂肪并用电子秤进行称重,并记录脂肪重量。全自动生化分析仪检测:血清甘油三酯(triglyceride,TG)、血清总胆固醇(totalcholesterol,TC)、血清高密度脂蛋白(high-densitylipoprotein,HDL)、血清低密度脂蛋白(low-densitylipoprotein,LDL)。取肝脏甲醛固定、HE和油红O染色检测肝脏脂肪沉积情况;比色法检测肝脏MDA和SOD含量; Realtime PCR检测相关指标的基因表达:IL-6、IL-8、TNF-α、乙酰辅酶A羧化酶(acetyl-Co A carboxylase, ACC)、固...