抗蝮蛇毒磷酸二酯酶单克隆抗体的制备及鉴定

从蝮蛇毒中提取磷酸二酯酶,用此酶免疫BALB/c小鼠,取其脾细胞与小鼠骨髓瘤细胞Fo融合,以间接ELISA检测杂交瘤细胞培养上清液和小鼠腹水中的特异性抗体,其效价分别为1∶128和1∶51 200.抗原阻断试验结果表明,此抗体对蛇毒磷酸二酯酶具有特异性.该杂交瘤细胞株定为G_8,该株单抗属鼠IgG_(2a)亚型,经体外持续培养6个月,其分泌抗体性能稳定.     

Ebstein畸形82例心血管造影分析

报道我院1958～1992年期间的 Ebstein 畸形82例。平片表现可归纳为球型、方型和不典型4种心脏形态。介绍该畸形的心血管造影方法,所见征象和相应的病理基础。主要造影征象有:1.心脏膈面双切迹征;2.帆样征:3.房化心室:4.三尖瓣闭锁不全。本文结合文献对本病病理,X 线表现进行了系统的讨论,并着重突出了我们的观点。     

两种根管弯曲度测量法间数学关系的推导

目的:研究2种根管弯曲度测量法测值(Schneider法的测值为θS,Weine法的测值为θW)间的数学关系。方法:将弯曲根管长轴简化为依次由相切的线段(长度为l1)、圆弧(半径为r、圆心角为θ)、线段(长度为l2)组成的连线,根据2测量法确定简化弯曲根管长轴测量角时不同的定点、连线方式,推导θS、θW间的函数方程以及与l1、l2、r、θ间的数学关系,分析方程与函数图形的特征。结果:θS与θW间存在以下数学关系:(1)tanθS=(1-cosθW ksinθW)/(sinθW kcosθW),k=l2/r;(2)θW/2≤θS≤θW。k取不同值时函数曲线的形态具有不同的特点。θW在0,π的区间内,θS与θW间呈近似的线性关系;k越大,θS越接近θW,k越小,θS越接近θW/2。结论:Schneider法与Weine法测值间存在着复杂的函数关系,根管根尖部直段的长度(l2)与弯曲段曲率半径(r)之比值是影响2种方法测值间差异的重要因素。     

Induction of FMLP-mediated calcium mobilization requires crosslinking of surface immunoglobulin in Daudi cells.

We have explored the requirements for the induction of the N-formyl-methionyl-leucyl-phenylalanine (FMLP) response in Daudi cells after anti-immunoglobulin treatment. Our results indicate that (a) induction of responsiveness to FMLP was observed in Daudi only after crosslinking of surface immunoglobulin by anti-immunoglobulin; (b) this induced responsiveness was not observed in Ramos or Wil-2 cells; (c) the F(ab')2 fragment was sufficient for the induction of the FMLP response, but the Fab fragment and the Fc fragment were ineffective; (d) of the many agents active in B lymphocyte regulation which were tested, none were as effective as anti-immunoglobulin in the induction of the FMLP response; and (e) three inhibitors of calcium mobilization (W-7 (N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide), PMA (phorbol 12-myristate 13-acetate), and colchicine), acting on distinct mechanisms, inhibited both the calcium mobilization due to anti-immunoglobulin and the induction of responsiveness to FMLP. Our results suggest important determinants in the induction of a calcium-mobilizing FMLP response in cells of B lymphocyte lineage include (a) the cell type, (b) a selective requirement for activation via surface immunoglobulin, and (c) crosslinking of the surface immunoglobulin.     

国人新生儿心脏的解剖学研究

本文对已固定的50例(男30.女20)新生儿心脏外径、重量、体积、内部结构等进行了观测,取得了有关心脏的大量数据,为年龄解剖学和临床提供资料。     

培养更多高质量的妇产科医师

1955年我毕业于同济医学院，留校工作至今已52年，一直从事医疗工作。师承全国著名的金问淇教授和蔡桂茹教授，金教授的广博学识和敏锐思维；蔡教授的精湛医疗水平和技术，对我的从医的道路有很大的影响。目睹同辈或下一代很多优秀的妇产科医师的成长、发展过程直到取得杰出业绩，会经常思考“如何做好妇产科医师”这一问题。     

Althin/TIAN System1000 HDF血透机常见故障及检修

Althin／TIAN System1000 HDF血透机是美国Baxter公司较为先进的血液透析滤过装置。它采用的是平恒腔式的定容超滤控制系统．使得超滤量的控制更为精确。非在线式血液滤过的设计彻底保证了滤过液的安全性。人性化的液晶触摸屏给操作和维修带来及大的方便。由于血透机特殊的工作环境．年久的机器会出现一些问题。现将几例常见故障的检修提供给同行，供同行参考。     

乳腺囊性增生病癌变过程中部分因素变化的意义

检测乳腺囊性增生病（ＦＣＤ）经不典型增生到癌变部分因素的变化。结果提示：从因明显ＦＣＤ症状活检至癌变为２～１０年；从Ⅱ级以上不典型增生到临床癌变需２～７年；癌变率为３．１％。ＦＣＤ患者存在性激素分泌调控失常，血浆雌激素和催乳素含量增加，导致上皮细胞增生。乳腺一般性增生细胞的ＤＮＡ含量和超微结构与正常乳腺上皮细胞相似；无肿瘤相关抗原及异常基因产物表达。而发生在一般性增生基础上的不典型增生则呈现细胞基因物质ＤＮＡ含量增加，部分为超４Ｃ的多倍体细胞；同时出现细胞膜和细胞核超微结构异常；雌激素受体含量增加，对性激素的依赖性和敏感性增强；部分不典型增生细胞出现胚胎性肿瘤相关抗原和异常基因产物表达。随不典型增生程度加重至乳腺癌，上述诸因素的变化趋势具有明显规律性。提示ＦＣＤ上皮细胞从一般性增生经不典型增生至乳腺癌为细胞生物学连续逐渐变化的过程。部分不典型增生细胞中具有癌倾向的细胞生物学行为异常和表型变化与乳腺癌发生密切相关。细胞核ＤＮＡ含量等异常变化及程度可作为乳腺癌前病变发展程度的客观标志     

Evaluation of peripheral blood involvement of mantle cell lymphoma by fluorescence in situ hybridization in comparison with immunophenotypic and morphologic findings.

Mantle cell lymphoma is non-Hodgkin's B-cell lymphoma characterized by the t(11;14)(q13;q32) translocation. Peripheral blood involvement of mantle cell lymphoma is usually associated with a poor prognosis and therefore, its identification is clinically important. In this study, we performed cyclin D1/IgH-probe fusion fluorescence in situ hybridization analysis on 223 peripheral blood samples: 185 from 125 mantle cell lymphoma patients, and 38 normal controls. The cutoff values for the test were established using normal controls. Flow cytometry on peripheral blood and corresponding bone marrow samples was used to evaluate this test. In all, 26% of the 185 peripheral blood samples and 27% of the 161 corresponding bone marrow samples were flow cytometry positive for mantle cell lymphoma. The mean numbers of single and- double-fusion signals and the mean number of CD5/CD19-positive cells, absolute blood lymphocyte count, and white blood cell count were significantly higher in peripheral blood and corresponding bone marrow samples with mantle cell lymphoma-positive flow cytometry. Double-fusion signals were more specific than single-fusion ones. Fluorescence in situ hybridization was far more likely to be positive for mantle cell lymphoma when the peripheral blood and the corresponding bone marrow samples had positive flow cytometry results or morphology (P<0.01). Our study indicates that cyclin D1/IgH-fusion fluorescence in situ hybridization analysis could be used to determine the presence and character of circulating mantle cell lymphoma cells in peripheral blood, thus enhancing our ability to evaluate leukemic mantle cell lymphoma and minimum residual disease.