A morphological study of the M-protein of Sendai virus.

A purification scheme is described for the M-protein of Sendai virus and an electron microscope study of the isolated protein is presented. The protein exists as subunits of 6 nm in diam., which possess a central hole; the subunits may be dimers of the polypeptide. They are able to form filamentous aggregates which wind around one another to form a helical structure. It is suggested that these filaments may be the form adopted by the protein in the virus, the filaments lying parallel to one another just beneath the virus membrane to form a shell, but that the helical form is likely to be a property only of the isolated protein.     

Potentiation of carbon tetrachloride-induced hepatotoxicity by 1,3-butanediol.

The hepatotoxic respone to a small acute challenge dose of carbon tetrachloride was evaluated in rats following production of metabolic ketosis by 1,3-butanediol (BD) administration. Neither BD alone or CCl₄ alone produced an appreciable degree of hepatotoxicity. In contrast, CCl₄ administration produced marked hepatotoxicity in rats rendered ketotic by BD.     

Blunt injury to intrahepatic vena cava and hepatic veins with survival

Injuries to the intrahepatic vena cava and hepatic veins are extremely lethal, particularly when caused by blunt trauma. Repair of the vena cava and hepatic veins often will require liver resection before adequate exposure can be obtained. To prevent lethal hemorrhage during resection and vascular repair, total vascular isolation of the liver may be necessary. Anoxic injury under these circumstances may be minimized by local hypothermia. The two patients reported here were successfully treated by direct suture of the vascular injuries without hepatic resection; however, vascular isolation was utilized in one patient.