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981.
Steinfeld R Heim P von Gregory H Meyer K Ullrich K Goebel HH Kohlschütter A 《American journal of medical genetics》2002,112(4):347-354
We examined 26 individuals with clinical and electron microscopic signs of late infantile neuronal ceroid lipofuscinosis (LINCL). In 22 cases, we found both pathogenic alleles. Sixteen patients exclusively carried either one or a combination of the two common mutations R208X and IVS5-1G > C. In the remaining cases, four missense mutations could be detected, of which R127Q, N286S, and T353P represent novel, previously not described alleles. A clinical performance score was developed by rating motor, visual, and verbal functions and the incidence of cerebral seizures in 3-month intervals during the course of the disease. A Total Disability Score was derived by summing up the single scores for motor, visual, and verbal functions. The 16 individuals with the two common mutations were grouped together (referred to as standard patients), and the 5th, 50th, and 95th centiles were calculated and graphically depicted over time. The scores for motor function and language ability dropped earliest and progressed very similarly in the standard patients. The performance curves of two children with the N286S mutation slightly diverged from the 95th centile. However, the performance curves of one patient with atypical LINCL carrying the R127Q mutation fell far beyond the 95th centile. The presented performance rating clearly and quantitatively delineates the disease course of the LINCL patients and hence offers a useful tool for clinical evaluation of future therapeutic interventions. In addition, the described performance score system can be applied to other types of neuronal ceroid lipofuscinoses and could be adapted to various other neurodegenerative diseases of childhood. 相似文献
982.
Wollmer MA Papassotiropoulos A Streffer JR Grimaldi LM Kapaki E Salani G Paraskevas GP Maddalena A de Quervain D Bieber C Umbricht D Lemke U Bosshardt S Degonda N Henke K Hegi T Jung HH Pasch T Hock C Nitsch RM 《Psychiatric genetics》2002,12(3):155-160
Tissue inhibitor of metalloproteinases 1 (TIMP-1) inhibits several proteinases including a disintegrin and metalloproteinase 10 (ADAM10), a major alpha-secretase that cleaves the beta-amyloid precursor protein within its amyloidogenic Abeta domain. The gene encoding TIMP-1 (TIMP 1) maps to the short arm of the X chromosome, in a region previously suggested as conferring genetic susceptibility for Alzheimer's disease (AD). To determine whether genetic variability of TIMP 1 contributes to the pathogenesis of AD, we analysed one single nucleotide polymorphism within TIMP 1 and one single nucleotide polymorphism in the 5'-untranslated region of TIMP 1 in patients with AD and control subjects from two independent and ethnically different populations. We did not observe any association between TIMP 1 genotypes and the diagnosis of AD in men or women. We also measured TIMP-1 protein levels in the cerebrospinal fluid of patients with AD, healthy control subjects, and patients with other neurological disorders. TIMP-1 levels were similar in all groups. In addition, no significant differences were observed after stratification for TIMP 1 genotypes. Our data show that neither genetic variability nor protein levels of TIMP-1 are associated with AD. 相似文献
983.
Valvatne H Steinsland H Sommerfelt H 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2002,110(9):665-672
A considerable proportion of enterotoxigenic Escherichia coli (ETEC) do not possess identifiable colonization factors (CFs). Genetic fingerprint analyses based on repetitive sequence-based polymerase chain reaction (rep-PCR) showed that 9 of 10 such CF-negative isolates which produced the thermolabile and the porcine thermostabile enterotoxin could be divided into three clusters. Following transformation with a plasmid harbouring the gene encoding CfaR, a positive regulator for several ETEC adhesins, three of the six strains in the first cluster expressed coli surface antigen 20 (CS20). No CFs were identified on the two transformed strains in the second cluster while the transformants of the two strains in the last cluster expressed CS12, the N-terminal amino acid sequence of which was deciphered. The study illustrates the potential of using genetic fingerprinting to group ETEC into clusters of strains with genes encoding different CFs and confirms the ability of CfaR to induce the expression of several different CFs. 相似文献
984.
985.
Chin Han Chan Claudia Kummerlwe Hans‐Werner Kammer 《Macromolecular chemistry and physics.》2004,205(5):664-675
Summary: Blends of high molecular weight poly(R‐3‐hydroxybutyrate) (PHB) ( = 352 000 g · mol?1), comprising of either low molecular weight poly(R‐3‐hydroxybutyrate) (D‐PHB) ( = 3 900 g · mol?1) or poly[(R‐3‐hydroxybutyrate)‐co‐(R‐3‐hydroxyvalerate)] (PHBV) ( = 238 000 g · mol?1) with 12 mol‐% hydroxyvalerate (HV) content as a second constituent, were investigated along with the thermal properties and morphologies. After isothermal crystallization, a lowering of the melting temperature of PHB can be observed with increasing content of the second component in the blends. This behavior points towards miscibility of the constituents both in the liquid and the solid state. Crystallization kinetics was studied under isothermal and non‐isothermal conditions. The overall kinetics of isothermal crystallization was analyzed in terms of the Avrami equation. Only one crystallization peak is observed in all cases for the PHB/D‐PHB and PHB/PHBV blends under the conditions studied. This demonstrates co‐crystallization of the constituents. The addition of D‐PHB or PHBV to PHB reduces the rate of crystallization of the blends compared to that of neat PHB. The corresponding activation energies of crystallization also decrease with an increasing concentration of the second constituent. Non‐isothermal crystallization, carried out with different cooling rates held constant, is discussed in terms of a quasi‐isothermal approach. The corresponding rate constants as functions of reciprocal undercooling show Arrhenius‐like behavior in a certain range of temperatures. At sufficiently high undercooling, the rate constants of crystallization for the isothermal process exceed those reflecting non‐isothermal conditions, whereas in the limit of low undercoolings, the rate constants become similar. Ring‐banded morphologies are observed when PHB is in excess. When the respective second component is the major component, fibrous textures of the spherulites develop.
986.
Carsten Frenz Andreas Fuchs Hans‐Werner Schmidt Ulrich Theissen Dietrich Haarer 《Macromolecular chemistry and physics.》2004,205(9):1246-1258
Summary: Diblock copolymers with a photoaddressable dispersed phase containing p‐methoxy substituted azobenzene side groups and a polystyrene matrix were synthesized and characterized. The block copolymers were prepared by a sequential living anionic polymerization of butadiene and styrene. The poly(1,2‐butadiene) segment was hydroborated and the hydroxy‐functions converted by a polymeranalogous reaction with the azo chromophore as side groups. The block copolymers were synthesized with different compositions by varying the length of the polystyrene segment and the length of the functionalized segment in order to obtain different morphologies. In this paper, for the first time a comparison of the cis‐trans photo‐isomerization behavior and photoaddressing with respect to different morphologies of the block copolymers is presented. To complete the comparison, the corresponding homopolymer and a statistical copolymer were also synthesized and investigated. A different photoaddressing behavior between homopolymer, statistical copolymer and the block copolymers was observed. One principal difference and advantage for photo addressable block copolymers is the lack of a formation of surface gratings which occurs in homopolymers and statistical copolymers.
987.
Characterization, expression and complex formation of the murine Fanconi anaemia gene product Fancg 总被引:3,自引:0,他引:3
van de Vrugt HJ Koomen M Berns MA de Vries Y Rooimans MA van der Weel L Blom E de Groot J Schepers RJ Stone S Hoatlin ME Cheng NC Joenje H Arwert F 《Genes to cells : devoted to molecular & cellular mechanisms》2002,7(3):333-342
BACKGROUND: Fanconi anaemia (FA) is an autosomal recessive chromosomal instability disorder. Six distinct FA disease genes have been identified, the products of which function in an integrated pathway that is thought to support a nuclear caretaker function. Comparison of FA gene characteristics in different species may help to unravel the molecular function of the FA pathway. RESULTS: We have cloned the murine homologue of the Fanconi anaemia complementation group G gene, FANCG/XRCC9. The murine Fancg protein shows an 83% similarity to the human protein sequence, and has a predicted molecular weight of 68.5 kDa. Expression of mouse Fancg in human FA-G lymphoblasts fully corrects their cross-linker hypersensitivity. At mRNA and protein levels we detected the co-expression of Fancg and Fanca in murine tissues. In addition, mouse Fancg and Fanca proteins co-purify by immunoprecipitation. Upon transfection into Fanca-deficient mouse embryonic fibroblasts EGFP-Fancg chimeric protein was detectable in the nucleus. CONCLUSIONS: We identified a murine cDNA, Fancg, which cross-complements the cellular defect of human FA-G cells and thus represents a true homologue of human FANCG. Spleen, thymus and testis showed the highest Fancg expression levels. Although Fancg and Fanca are able to form a complex, this interaction is not required for Fancg to accumulate in the nuclear compartment. 相似文献
988.
Quantitative expansion of structural genomic alterations in the spectrum of neuroendocrine lung carcinomas 总被引:3,自引:0,他引:3
Gugger M Burckhardt E Kappeler A Hirsiger H Laissue JA Mazzucchelli L 《The Journal of pathology》2002,196(4):408-415
The pathogenesis and interrelationships of neuroendocrine lung carcinomas are not well understood. Tissue macro-arrays prepared from surgical resection specimens from 35 patients with typical carcinoid (TC), six with atypical carcinoid (AC), 13 with large cell neuroendocrine carcinoma (LCNEC), and 15 with small cell lung carcinoma (SCLC) were investigated by fluorescence in situ hybridization (FISH) and immunohistochemistry. Hybridizations with locus-specific DNA probes demonstrated a high incidence of deletion for the tumour suppressor genes p53 and retinoblastoma (Rb), and for the oncogene cyclin D1, comparable in all carcinoma types. Similarly, an increase of DNA copy number for the Her-2/neu and c-myc oncogenes was noted in all neoplasms. A more detailed quantitative analysis of the results, however, demonstrated increasing numbers of cells harbouring these genomic alterations, from low-grade TC to highly malignant SCLC, with the exception of cyclin D1 deletion. Mutations of the p53 and Rb genes, as assayed by immunohistochemical studies, were observed at high incidence in high-grade carcinomas, compared with a low incidence in the low-grade carcinomas. Conversely, in all carcinoma types, neither membrane-bound Her-2/neu nor nuclear cyclin D1 was detected. It is concluded that structural genomic alterations are frequent in neuroendocrine lung carcinomas and that their occurrence may be underestimated by immunohistochemical studies alone. The quantitative expansion of the Rb, p53, c-myc, and Her-2/neu alterations towards high-grade carcinomas suggests common pathogenetic mechanisms in the spectrum of these neoplasms. 相似文献
989.
Second family with hearing impairment linked to 19q13 and refined DFNA4 localisation 总被引:3,自引:0,他引:3
Mirghomizadeh F Bardtke B Devoto M Pfister M Oeken J König E Vitale E Riccio A De Rienzo A Zenner HP Blin N 《European journal of human genetics : EJHG》2002,10(2):95-99
Until now, over 30 loci have been identified by linkage analysis of affected families that segregate non-syndromic and dominantly inherited forms of hearing impairment (DFNA). A German family with a non-syndromic progressive hearing impairment transmitted in autosomal dominant mode was linked to 19q13.3-q13.4 by a genome-wide scan. Due to the low lod-score (1.85 at theta=0.05) for APOC2-locus we extended the fine mapping attempt with further markers in the same chromosomal region. This resulted in significant evidence for linkage to the markers D19S246 and D19S553 (two-point lod-score of 4.05 and 3.55 at theta=0.0) and a candidate critical region of 14 cM between markers D19S412 and D19S571. This region shows partial overlap with the previously reported DFNA4 critical region. The human gene BAX is orthologous to the rodent Bcl2-related apoptosis gene that is temporally expressed during the postnatal period in the developing inner ear of the mouse. BAX, mapping at a distance of no more than 0.73 cM distally to marker D19S553 appeared a likely candidate in our pedigree but genomic sequencing of coding regions and exon/intron boundaries excluded disease-related mutations. However, additional ESTs in the same region remain to be tested. 相似文献
990.
Effect of micro-roughness produced by TiO2 blasting--tensile testing of bone attachment by using coin-shaped implants 总被引:2,自引:0,他引:2
The aim of the present study was to examine bone response to micro-rough titanium implants. Forty coin-shaped implants were divided into eight groups according to their surface roughness. The first group had electropolished surfaces. The surfaces of implant groups 2-8 were blasted with TiO2 particles with incremental grain sizes ranging from 7.5-12.5 to 270-330 microns. Five implants from each group were placed into the cortical bone of the proximal tibia in New Zealand Black rabbits. To avoid bone overgrowth during the retention phase the implants were fitted into tight polytetrafluoroethylene (PTFE) caps leaving only the flat test surface exposed to bone. The healing period was set to 10 weeks, and implants with attached bone were evaluated using a tensile testing machine. In groups 1-7 a significant correlation between the micro-roughness of the implant surfaces and retention strength was observed. Maximum bone bonding was observed with implants blasted with 180-220 microns grain size (group 7). Blasting with larger TiO2 particles appeared to decrease the effect. The findings suggest that the best grain size of TiO2 particles for optimising retention of titanium implants in cortical bone should be in the 180-220 microns range. 相似文献