Diastematomyelia--clinical manifestation and treatment outcome.

Diastematomyelia is a rare congenital anomaly characterized by a division of the spinal cord or the filum terminale into two parts. In Korea, only one case has been reported. The authors have operated on 5 cases of diastematomyelia with septum since July, 1978. The ages ranged from 1 to 44 years (median; 11 years). There were 2 boys, 2 girls and an adult man. The disease manifested by cutaneous abnormalities and neurological or orthopedic deficits. Pain was a chief complaint in the adult patient. The symptoms had progressed in 3 cases. The diagnosis was made correctly by CT myelography or MRI in 4 cases. The median septum was located at the lumbar area in 4 cases and at the lumbosacral region in 1 case. Associated abnormalities included low lying conus (5 cases), lipoma (2 cases), thickened filum terminale (1 case), hemilipomyelomeningocele (1 case) and syrinx (1 case). The median septum was removed. The dural sleeve adjoining the septum was resected and the dural sac was reconstructed. The role of MRI in the diagnosis and planning of surgery and the high frequency of associated low lying conus were emphasized. Though the surgical treatment relieved pain, it did not reverse the neurological deficits or orthopedic deformities significantly, which suggests the beneficial effects of early surgical intervention in the cases with progressive symptoms.     

纯化的结核杆菌多肽抗原刺激人γδT细胞的效应分析

目的：探讨从结核杆菌多肽抗原(Mtb-Ag)纯化的多肽(C主肽)对人新鲜外周血γδT细胞的促增殖效应以及对已活化扩增的T细胞再次刺激的激活效应。方法：采用不同剂量的C主肽体外刺激健康人外周血PBMC，培养10天时经流式细胞仪检测细胞表型；另外以γδT细胞活化标志分子CD69的表达为指标，分析C主肽对已被Mtb-Ag激活扩增13天的γδT细胞再次刺激的激活效应。同时以MTT法检测C主肽的再刺激对已活化扩增的γδT细胞的促增殖活性。结果：结核杆菌C主肽对人新鲜的γδT细胞具有显著扩增效应；当C主肽再刺激已经活化的γδT细胞时，可使其显著表达CD69分子，同时对γδT细胞具有显著促增殖活性。结论：结核杆菌C主肽可能是Mtb-Ag发挥特异性激活人γδT细胞的有效成分。     

抗骨肉瘤药物纳米载体的研究进展

骨肉痛是最常见的恶性骨肿瘤,病死率较高。而生物医用纳米材料是纳米材料和生物材料交叉的一个全新领域,在生物医学上有着十分诱人的、广泛的应用前景。本文对纳米无机生物材料、纳米高分子生物材料、纳米复合生物材料作为抗骨肉瘤药物载体的研究进展作了较全面的综述。     

Nucleotide sequence of a Japanese isolate of Squash mosaic virus. Brief report

Summary.  The complete nucleotide sequence of the RNA-1 of Squash mosaic virus (SqMV) was determined using a Japanese isolate (Y-SqMV). The sequence consisted of 5865 nucleotides excluding the poly (A) at the 3′ terminus and contained a single long open reading frame with a coding capacity for a protein of M_r209971. Analysis of the deduced amino acid sequence suggested a genomic organization typical of comoviruses. The nucleotide sequence of the RNA-2 of Y-SqMV was also determined and compared with the SqMV isolates from the United States. The larger and smaller capsid protein (CP) coding region was compared to those of K-SqMV and Z-SqMV, which represent two subgroups of SqMV. The larger CP gene of Y-SqMV showed 93.0% and 88.0% identities with those of K-SqMV and Z-SqMV, respectively at the nucleotide level. The smaller CP gene of Y-SqMV was 94.1% and 88.4% identical with those of K-SqMV and Z-SqMV. The results suggested that the Japanese SqMV isolate (Y-SqMV) is distinct from those in the United States, and might represent a third subgroup. Received May 7, 2001 Accepted August 1, 2001     

Recombinant Helicobacter bilis Protein P167 for Mouse Serodiagnosis in a Multiplex Microbead Assay

Infection of mice with Helicobacter bilis is widespread in research and commercial mouse colonies. Therefore, sensitive, specific, and high-throughput assays are needed for rapid and accurate testing of mice in large numbers. This report describes a novel multiplex assay, based on fluorescent microbeads, for serodetection of H. bilis infection. The assay requires only a few microliters of serum to perform and is amenable to a high-throughput format. Individual microbead sets were conjugated to purified, H. bilis-specific, recombinant proteins P167C and P167D and bacterial membrane extracts from H. bilis and Helicobacter hepaticus. For detecting H. bilis infection in the microbead multiplex assay, P167C and P167D provided significantly higher sensitivities (94 and 100%, respectively) and specificities (100 and 95%, respectively) than membrane extract (78% sensitivity and 65% specificity). Microbead multiplex assay results were validated by enzyme-linked immunosorbent assay. Purified recombinant proteins showed low batch-to-batch variation; this feature allows for ease of quality control, assay robustness, and affordability. Thus, recombinant antigens are highly suitable in the multiplex microbead assay format for serodetection of H. bilis infection.     

Flat depressed early colon cancer--a case report.

A flat depressed early colon cancer (FDEC) is characterized by non-polypoid growth pattern, no association of adenomatous tissues and a tendency of even small lesions toward submucosal invasion and lymph node metastasis. It supports de novo carcinogenesis of colorectal cancer, although most colorectal cancers arise in pre-existing adenoma (adenoma-carcinoma sequence). There have been few reports of small depressed cancers because of the difficulty in colonoscopic detection and the rapid development to ulcerating advanced cancers. We report a case of flat depressed early colon cancer confined to mucosa detected by indigo carmine contrast colonoscopy.     

咀嚼肌的应用解剖学研究——I.咬肌的动脉与神经

在30具(男23.女7)共60例成人尸体上观察与测量了咬肌的动脉与神经来源、咬肌神经的分支以及肌内构筑、咬肌由上颌动脉咬肌支和邻近动脉肌支供血,面动脉与面横动脉的咬肌支为其主要营养动脉.咬肌神经在肌内可分成单干和双干两型,前者居多,两型中其肌内分支间均有吻合.就结果对有关颌面外科应用问题进行了探讨.     

两种兔源内毒素结合蛋白的分离、纯化及其体外生物学活性的对比研究

目的　从烧伤合并内毒素血症致多器官损害家兔血清中分离纯化内毒素结合蛋白 ,并初步对比研究它们的生物学活性。方法　兔血清蛋白质通过二步离子交换层析 (Bio Rex 70Resin、Mono Q)及凝胶过滤分离纯化 ,并经流式细胞分析实验、绵羊红细胞凝聚实验、氨基末端氨基酸残基测序等方法鉴定 ,再将所获蛋白质作用于体外培养的人单核细胞 (U937) ,观测其分泌TNFα等细胞因子的生物效应。结果　获得两种具有促进内毒素 (LPS)与外周血单核细胞 (PBMC)结合的蛋白质 ,其中一种相对分子质量为 6 0× 10 4 ,其N端 10个氨基酸序列为TNPGLITRIT ,证实为兔内毒素结合蛋白 (LBP) ;另一种蛋白质相对分子质量为 4 8× 10 4 ,其N端 10个氨基酸序列为GSQGTFTSEE ,与NCBI蛋白质库中的氨基酸序列进行比较 ,未发现相同序列 ,命名为p48。结论　p48与LBP具有相似的生物学功能 ,p48的生物学活性稍弱于LBP。提示体内除LBP外 ,p48也同样起着介导LPS的毒性作用。这可能是近年国外研究中阻断LBP的活性后 ,机体毒性反应并无明显减轻的原因之一。     

LPS对大鼠肺微血管内皮细胞SSeCKS的表达影响

目的研究细菌脂多糖（LPS）对大鼠肺微血管内皮细胞（rat pulmonary microvascular endothelial cell，RPMVEC）Src抑制的蛋白激酶C底物（Src-suppressed C kinase substrate，SSeCKS）表达和细胞内定位的影响，探讨SSeCKS参与细胞骨架结构改变的可能机制。方法用植块培养法体外培养大鼠肺微血管内皮细胞，用抗大鼠CD31抗体进行细胞鉴定。LPS刺激体外培养的RPMVEC，用定量PCR、免疫印迹方法检测LPS刺激RPMVEC不同时间SSeCKS mRNA和蛋白的表达情况；用0．05μmol／L蛋白激酶C（PKC）抑制剂（Calphostin C）预处理RPMVEC30min后再用LPS刺激6h，免疫荧光细胞化学法观察Calphostin C对LPS诱导SSeCKS与纤维状肌动蛋白（filamentous—actin，F-actin）细胞内定位和结构改变的影响。结果定量PCR结果显示LPS刺激RPMVEC1h后SSeCKS表达水平达到最高，Westernblot结果与定量PCR结果相一致，同时，LPS以时间依赖的方式诱导SSeCKS磷酸水平增加。免疫荧光结果显示LPS刺激后，F-actin发生重构，细胞内形成应力纤维，SSeCKS向核周、细胞膜纤维、板状伪足末端聚集；Calphostin C部分抑制LPS对内皮细胞F-actin和SSeCKS细胞内定位改变的影响。结论LPS能够诱导内皮细胞SSeCKS表达增加和细胞内定位改变，PKC参与I娲诱导内皮细胞F-ac，6n的重构和SSeCKS重新分布；提示SSeCKS可能与LPS诱导内皮细胞F-actin的重构有关。     

核素唾液腺显像的定量分析软件开发与应用

目的开发定量分析唾液腺功能及其图象显示的程序。方法应用Aladdin语言编写ROI模块提取各唾液腺及甲状腺区域的摄取数据及本底,用曲线函数生成各ROI的时间-放射性活度曲线,对曲线数据处理,计算唾液腺摄取、排泌指数等指标,然后把图象、曲线及计算结果显示在一个模板内。结果同一幅输出界面图可显示唾液腺的形态、ROI示意图、唾液腺的摄取与排泌功能数值及其曲线,以标准化的显示格式提供给临床医师。不同操作者处理同一资料的功能指标结果基本一致,软件计算的功能指数与图象显示结果及临床诊断基本相符。结论开发的唾液腺功能定量分析软件,程序运行稳定,结果重复性好。