正确鉴别甲型H1N1流感与普通感冒

正确鉴别甲型H1N1流感与普通感冒,有助于全民普及卫生知识,培养对待流行病的科学态度,增强防病意识,养成良好的卫生习惯,有助于鉴别诊断和科学应对疫情.     

PETHEMA ALL-93研究结果:成人高危急性淋巴细胞白血病缓解后维持治疗、异基因和自体造血干细胞移植的疗效比较

4背景 近年，成人急性淋巴细胞白血病（acute lymphoblastic leukemia，ALL）诱导完全缓解（complete remission，CR）率可达80％～85％．5年无病生存率（diseases-free survival，DFS）30％-40％。成人ALL预后的改善可能与采用了包括以大剂量甲氨蝶呤联合阿糖胞苷的强烈巩固治疗方案有关．并与根据疾病危险度进行分级分层治疗、根据疾病亚型制定个体化的治疗方案有关。     

肝移植术后早期黄疸的原因分析

目的 探讨肝移植术后早期黄疸的原因。方法 回顾性分析87例肝移植患者术后早期黄疸发生的原因及临床特征。结果 87例患者中，术后46例（52．87％）发生黄疸，共发生21种并发症，有17种（80．95％）并发症伴发黄疸，导致黄疸发生的常见原因依次为缺血．再灌注损伤（n=25，28．73％）、胆道并发症（n=23，26．44％）、急性排斥反应（n=19，21．84％）、药物性肝损害（n=4，4．60％）、移植肝病毒感染（n=4，4．60％）、腹腔内或全身严重感染等（n=3，3．45％），有2例（2．30％）原因不明；46例中，部分病例同时存在多个原因。不同原因所致的黄疸，在发生时间、胆红素水平的变化等方面均呈现相应的特征。结论 黄疸是肝移植术后早期较常见的临床表现，导致黄疽的原因复杂，甄别其主要原因，对指导治疗至关重要。     

NF-κB靶向性哑铃形“圈套”寡核苷酸对体外肾小球系膜细胞细胞因子表达的影响

目的:设计合成靶向NF-κB的哑铃形“圈套”ODNs,并检测其对NF-κB转录活性和肾小球系膜细胞细胞因子(TNF-α和IL-6)表达的抑制效应。方法:以NF-κB顺式作用元件κB序列为模板,设计包含两个拷贝的κB序列,长度为58个碱基的环状ODNS,合成后部分序列做FAM标记,行转集效率鉴定实验。采用电泳迁移率改变实验(EMSA)体外检测哑铃形圈套ODNs对NF-κB转录活性的抑制效应。提取大鼠肾小球系膜细胞,随机分为正常对照组、LPS刺激组、哑铃形圈套ODNs处理组、无关圈套ODNs处理组和脂质体处理组。采用阳离子脂质体将2、4、8mg/L不同剂量哑铃形圈套ODNs转染大鼠肾小球系膜细胞,6h后用LPS刺激,收集转染后8、12、18上清和细胞。用酶联免疫吸附试验(ELISA)法检测上清细胞因子蛋白表达,逆转录PCR(RT-PCR)法检测细胞因子mRNA转录。结果:阳离子脂质体可将哑铃形圈套ODNs高效转集入肾小球系膜细胞,哑铃形圈套ODNs在体外能有效地抑制NF-κB与其顺式元件的结合;4、8mg/L剂量哑铃形圈套ODNs可明显抑制LPS诱导的大鼠肾小球系膜细胞TNF-α和IL-6转录与表达。结论:靶向N...     

桡骨远端骨折180例诊断分析

本文分析我院1997-2000年180例诊断、诊疗结果并对并发症进行讨论. 　　1 临床资料 　　1.1 病例 180例中男84例,女96;年龄18～89岁.左侧76例,右侧98例,双侧6例,皆因外伤.……     

人毛乳头细胞凝集性生长差异表达基因cDNA文库的构建

目的 构建毛乳头细胞凝集性生长状态下差异表达基因的消减cDNA文库。方法 分别提取凝集性生长和非凝集性生长状态下毛乳头细胞中的总RNA，应用SMART cDNA合成技术和抑制性消减杂交技术分离毛乳头细胞凝集性生长状态下差异表达基因的cDNA片段并建立消减cDNA文库；利用PCR对随机挑选的100个白色菌落进行插入片段的验证，对其中证实有插入片段的30个克隆进行cDNA斑点杂交验证。结果 所构建的消减cDNA文库扩增后得到320个阳性克隆，随机挑选的100个阳性克隆中95％的均有长度在100—600bp的插入片段，cDNA斑点杂交验证显示27个(90．0％)克隆为阳性。结论 结合SMART cDNA合成技术，应用抑制性消减杂交成功地构建了毛乳头细胞凝集性生长差异表达基因消减cDNA文库，为进一步筛选和克隆毛乳头细胞凝集性生长相关基因奠定了基础。     

Inhibition on Telomerase Activity and Cytotoxic Effects by Cisplatin in Cultured Human Choroidal Melanoma Cells

Purpose: To study the changes of telomerase activity and cytotoxic effects by Cisplatin; cis-dichlorodiamine platinum (CDDP) in cultured human choroidal melanoma. Material and Methods: The primary cultured human choroidal melanoma cells were cultured in the presence and absence of CDDP with different concentration and time respectively. The toxic effects were evaluated by MTT and the level of telormarse was detected by PCR-ELISA assay. And the relationship between telomerase activity and cytotoxic effects were analyzed by a correlation analysis.Results: Following the increase of the concentration and the time of CDDP, gradually repressed telomerase activity was detected in cultured cells. Meanwhile, the restrain rate of the cells increased. The telomerase activity at 24h and 1μg/ml was repressed significantly compared with the control cells. However, the appearance of cell death lagged behind the decreasing of telomerase.Conclusions: CDDP is an effective telomerase inhibitor in cultured choroidal melan     

Psychoactive substance use in three sites in China: gender differences and related factors

One year prevalence rates far psychoactive substance use are reported based on a community survey in Hunan, Helongjiang and Jiangsu. Data were gathered on more than 14 000 respondents (15–65 years old). The drinking rates ranged from 58.3% to 82.6% for men and from 16.3% to 31.4% for women. Smoking rates ranged from 64.9% to 68.1% for men and from 0.1% to 20.5% for women. Most drinkers were light users and most smokers were heavy users, Illicit drug use was found in the Hunan site. For both frequency and quantity of psychoactive substance use, women are lighter consumers than males (except for the use of minor tranquilizers and analgesics). Psychosocial factors related to drinking and smoking are also investigated in this study.     

LncRNA-MAFG-AS1在结直肠癌中的表达及临床意义

目的 探讨LncRNA-MAFG-AS1在结直肠癌中的表达及临床意义。方法 选取我院行手术切除的结直肠癌组织及对应的癌旁正常组织各80例,应用定量即时聚合酶链锁反应(quantitative real time polymerase chain reaction, qRT-PCR)检测结直肠癌组织及癌旁正常组织中LncRNA-MAFG-AS1的表达水平,并分析其与结直肠癌临床病理特征之间的关系,所有患者随访截止日期到2019年12月31日,应用Kaplan-Meier(K-M)法进行生存分析,采用Cox回归模型分析结直肠癌预后的危险因素。结果 结直肠癌组织中LncRNA-MAFG-AS1相对表达量为2.398±0.214,高于癌旁正常组织的1.032±0.132,差异有统计学意义(P<0.05)。当患者TNM分期更高、中高分化及存在淋巴结转移和远期转移时,LncRNA-MAFG-AS1过表达(P<0.05)。K-M生存分析显示高表达患者无进展生存时间(progression-free survival, PFS)为(20.36±3.21)个月,低表达患者为(29.71±4....