抗人甲状腺球蛋白单克隆抗体的制备及其对抗原决定簇特异性的研究

用杂交瘤技术建立三株分泌抗甲状腺球蛋白(TG)单克隆抗体的杂交瘤细胞株。所获三种单克隆抗体经兔抗小鼠IgG亚类、IgM及IgA血清鉴定表明,两种为IgG_1,一种为IgA;三种含单克隆抗体小鼠腹水的滴度(ELISA法)均为51200以上;以竞争性固相抗体结合试验测定它们对抗原决定簇的特异性差异,结果发现三种单克隆抗体的抗原结合部位是不同的,但对抗原的结合有一定的相互竞争抑制现象。     

RESEARCH ON A REAL-TIME CONTROL SYSTEM OF LOW POWER DISSIPATION FOR CONTINUOUS CYCLIC PERITONEAL DIALYSIS(CCPD) CYCLER

FOREWORDPeritonealdialysisrepresentssoluteandfluidexchangemainlybetweenperitonealcapillarybloodanddialysissolutionintheperitonealcavity'CombinedwithhemodialySisandrenaltransplantation,peritonealdialysisisusedmainlyforthetreatmentofrenalfailure,particularlyforchronicfailure'In1976,Prof'Popovichdevelopedcontinuousambulatoryperitonealdialysis(CAPD)basingontheconceptofcontinuousequilibrationdialysis('1'Fromthenon,peritonealdialysisfounditsclinicaluse'Continuouscyclicperitonealdialysis(CCPD…     

心血管外科术后急性肾功能衰竭的处理

目的：总结两组心血管外科术后急性肾功能衰竭病例的治疗经验，提出心血管外科术后急性肾功能衰竭的特点、早期诊断标准及处理原则。方法：１７例心血管外科术后急性肾功能衰竭病例，以入院先后分为两组。第１组８例，第２组９例。回顾第１组病例的急性肾功能衰竭的治疗经验，总结腹膜透析、血液透析和血液滤过的优缺点。在第２组病例治疗中，以急性肾功能衰竭的早期诊断为前题，早期开始腹膜透析。同时，强调综合治疗的重要性。结果：第１组病例均死亡。第２组病例，７例存活（７８％）。结论：心血管外科术后急性肾功能衰竭的早期诊断和早期肾功能替代疗法，是治疗成败的关键。腹膜透析简便易行，在急性肾功能衰竭的早期开始腹膜透析，可以收到良好效果，并以此作为心血管外科术后急性肾功能衰竭的首选肾功能替代疗法。     

不同浓度氢氟酸对大鼠血钙变化的影响

OBJECTIVE: To evaluate the effects of different concentration of hydrofluoric acid (HF) on local skin and calcium level of serum. METHODS: SD rats were divided into two groups which were wounded by 20% and 40% percent of HF respectively. Samples of blood and wound tissue were harvested at different postburn time for the analysis of the calcium level of the serum and histological study. RESULTS: It was found that twenty percent of HF was enough to cause a skin damage, and might bring about fatal hypocalcemia after a prolonged contact. High concentration of HF (40%) could cause deep tissue necrosis within a short time, and result in a fatal hypocalcemia within 24 hour even in the case of a small area injury. CONCLUSION: It is important to treat the patient with HF injury as early as possible. Sufficient calcium must be applied guided by laboratory study in order to prevent the fatal hypocalcemia.     

Stress signaling through Ca2+/calmodulin-dependent protein phosphatase calcineurin mediates salt adaptation in plants

Calcineurin (CaN) is a Ca²⁺- and calmodulin-dependent protein phosphatase (PP2B) that, in yeast, is an integral intermediate of a salt-stress signal transduction pathway that effects NaCl tolerance through the regulation of Na⁺ influx and efflux. A truncated form of the catalytic subunit and the regulatory subunit of yeast CaN were coexpressed in transgenic tobacco plants to reconstitute a constitutively activated phosphatase in vivo. Several different transgenic lines that expressed activated CaN also exhibited substantial NaCl tolerance, and this trait was linked to the genetic inheritance of the CaN transgenes. Enhanced capacity of plants expressing CaN to survive NaCl shock was similar when evaluation was conducted on seedlings in tissue culture raft vessels or plants in hydroponic culture that were transpiring actively. Root growth was less perturbed than shoot growth by NaCl in plants expressing CaN. Also, NaCl stress survival of control shoots was enhanced substantially when grafted onto roots of plants expressing CaN, further implicating a significant function of the phosphatase in the preservation of root integrity during salt shock. Together, these results indicate that in plants, like in yeast, a Ca²⁺- and calmodulin-dependent CaN signal pathway regulates determinants of salt tolerance required for stress adaptation. Furthermore, modulation of this pathway by expression of an activated regulatory intermediate substantially enhanced salt tolerance.     

挫伤性脉络膜破裂出血

应用荧光血管造影方法观察118例眼挫伤病人的眼底改变。其中46例(39.0％)有脉络膜破裂去血。46例中视盘附近脉络膜破裂出血26例(56.5％);黄斑部脉络膜破裂出血13例(28.3％),其余部位脉络膜破裂出血7例(15.2％),三者间差异非常显著(P<0.01)。视盘颞侧部位脉络膜破裂出血22例(47.8％),鼻侧部位18例(39.1％),其余6例为视盘的上、下或环周破裂出血。     

固体废物处理和处置设施的选址

新建废物处理和处置设施的选址是一项综合性很强的工作，涉及自然环境，社会政治和经济、安全和技术等诸多方面，选址的主要目标通过一系列的选址技术的步骤来具体体现。     

新疆塔城地区多房棘球蚴的鼠类宿主考察

1989～1991年由新疆的塔城、裕民、额敏和托里等4个县市采集各类鼠类4科11属14种共9832只。剖检结果发现有0.014％(1/6980)小家鼠(Mus musculus)和0.09％(2/2211)赤颊黄鼠(Citellus erythrogenys)自然感染多房棘球蚴。二者发育的多房棘球蚴均系不育囊,囊内没有原头节。小家鼠和赤颊黄鼠均是本虫在我国的鼠类宿主新记录,而赤颊黄鼠又是本虫鼠类宿主的首次报道。     

Quantitative morphometry and apotosis of colorectal carcinoma

目的：探讨大肠疾病形态计量学和凋亡规律。方法：应用图像自动分析系统，检测了大肠疾病形态计量学参数和凋亡指数（AI）。结果：大肠腺癌AI明显高于大肠腺瘤和炎性增生组（P分别＜O．05和＜O．001）；细胞核面积、周长、凸状周长、等效直径、周长面积因子和核平均灰度六项参数，在腺癌组明显高于腺瘤和炎性增生，而灰度标准差明显低于其他两组，大肠腺癌AI与PM2／A相关系数为O．51（P＜0．05）。结论：形态计量学参数和凋亡指数在鉴别大肠疾病中具有一定参考价值。     

高原肺心病急性发作期286例临床特点分析

本文对高原地区(海拔2260～3200m)286例慢性肺心病急性发作期病人作了临床特点分析。结果表明:①PaO_2和PaCO_2(分别为5.38±0.98和6.20±1.09kPa)均明显低于平原肺心病;②肺性脑病并发率(3.8％)较平原低;③血液粘度较平原高,高粘血症组呼衰、右心衰和肾功异常的发生率(分别为94.5、84.6、29.2％)明显高于血粘度正常组(分别为52.3、45.2、14.2％)。对其机理和临床意义进行了讨论。