Adrenal cortical carcinoma with hepatic metastasis: preoperative radiological evaluation

Preoperative evaluation of a patient with adrenal carcinoma and hepatic metastases was performed by arteriography, computed tomography (CT) and computed tomographic arteriography (CTA). Early and complete demonstration of tumor involvement by these imaging modalities allowed an accurate assessment of tumor extension and subsequent aggressive surgical removal of the primary as well as the metastasis in the liver.     

Characterization of an acidic polysaccharide from the seeds of Malva verticillata stimulating the phagocytic activity of cells of the RES

An acidic polysaccharide, designated as MVS-IVA, was isolated from the seeds of Malva verticillata. It showed a remarkable reticuloendothelial system potentiating activity in the carbon clearance test. Chemical and spectroscopic studies demonstrated that the polysaccharide is rich in alpha-L-arabinofuranose residues and possesses mainly an alpha-1,5-linked L-arabino-beta-3,6-branched-D-galactan structure accompanied with rhamnogalacturonan type units. Both alpha-1,3-linked L-arabinopyranose and beta-1,4-linked D-xylopyranose residues were also identified as component units. MVS-IVA contains a small amount of protein (3.3%).     

Towards a genetically validated new affective temperament scale: a delineation of the temperament phenotype of 5-HTTLPR using the TEMPS-A

BackgroundAlthough it has been described that affective temperaments are associated with the 5-HTTLPR, less attention was paid to the association between this polymorphism and subscales and items related to each affective temperament. The aim of our study was to investigate the association of affective temperament subscales and individual items with the s allele of the 5-HTTLPR.Method138 psychiatrically healthy women completed the TEMPS-A questionnaire and were genotyped for 5-HTTLPR. Scores of subjects on the temperament scales, subscales and items in the three genotype and the two phenotype groups were compared using ANOVA. We selected items with significantly different mean scores between the three genotype groups and the two phenotype groups separately and performed item analysis.ResultsSubjects in the different 5-HTTLPR genotype and phenotype groups have significantly different score on scales measuring depressive, cyclothymic, irritable and anxious temperaments, and several subscales composing these temperamental scales. Subjects in the three genotype groups scored significantly different on 11 items, 8 of these remained in a derived genotype scale after item analysis. Subjects in the two phenotype groups had significantly different scores on 12 items, 9 of them were retained in a derived phenotype scale after item analysis.LimitationsOur sample was relatively small and included only women.ConclusionsOur data provide support for the association of affective temperaments with the s allele. Although the cyclothymic temperament shows the strongest association, all temperaments within the depressive superfactor have a similar share in this association. The newly derived 5-HTTLPR Phenotype Scale shows strong association with 5-HTTLPR genotype and phenotype, therefore this scale should be further investigated in relation to psychiatric disorders, as well as psychological traits and temperaments.     

Affective temperaments and self-harm in adolescents: A cross-sectional study from a community sample

Background

Adolescent self-harm (SH) is a major health problem potentially associated with poor outcomes including reduced life expectancy and risk of completed suicide in adulthood. Several studies point to the role of possible constitutional vulnerabilities that could predispose to this behavior. This study sets out to assess the relationship between SH and affective temperaments (AT) in adolescents.

Methods

A cross-sectional sample of public school students (n=1713), with age limits between 12 and 20, was examined using anonymously completed self-report instruments including ‘The Lifestyle & Coping Questionnaire’ and the ‘Temperament Evaluation of Memphis, Pisa, Paris and San Diego—auto-questionnaire’ (TEMPS-A). SH was defined according to strict criteria through a two-stage procedure. Statistical significance of associations with SH for categorical variables was assessed in bivariate analysis. AT predictors of lifetime SH were examined in multivariate logistic regression analyses.

Results

Lifetime SH was reported by 7.3%, being about three times more frequent in females. SH was associated, in both genders, with a significant deviation on depressive, cyclothymic and irritable dimensions of TEMPS-A. After multivariate logistic regression adjusted to family typology, smoking status, alcohol and drug consumption, only depressive temperament remained significantly associated as a predictor of SH in both genders.

Limitations

The use of self-rating instruments and the cross-sectional nature of the study limit our results.

Conclusions

Cyclothymic, irritable and especially depressive temperament might represent an important marker of vulnerability to SH in both male and female adolescents.     

A transmembrane chemokine, CXC chemokine ligand 16, expressed by lymph node fibroblastic reticular cells has the potential to regulate T cell migration and adhesion

Stromal cells in lymphoid tissues provide microenvironmental fields required for the triggering of efficient immune responses. Fibroblastic reticular cells (FRCs) are one of the integral constituents of such stromal fields; they construct the reticular network and are considered to regulate immune cells' behavior. However, the factors that mediate the interaction between lymphocytes and FRCs are poorly understood. Here we show that a mouse lymph node (LN)-derived FRC cell line, BLS4, expresses a transmembrane chemokine, CXC chemokine ligand (CXCL) 16, in response to tumor necrosis factor alpha (TNFalpha) and IFNgamma. TNFalpha-induced expression of CXCL16 depends on NFkappaB, p38 MAPK and PKA. Matrix metalloproteinase activity is required for producing soluble CXCL16 in the culture supernatant, likely via shedding at the juxtamembrane region of the extracellular domain. IL-12 enhances the expression of CXCR6 in anti-CD3/CD28-stimulated CD8+ T cells and their adhesion to the BLS4 cell surface in a TNFalpha-dependent fashion. The adherence is significantly inhibited in the presence of both anti-CXCL16 and anti-vascular cell adhesion molecule 1 (VCAM-1) antibodies. CXCL16 expression is also detected in the FRCs in LN sections and in gp38+VCAM-1+ FRCs isolated from LNs. Taken together, these findings suggest that CXCL16 is an important mediator of lymphocyte-stromal interaction within lymphoid tissues.