TNFα-Tumstatin45-132分子构建、生物学特征预测和活性验证

目的 构建人重组TNFα-Tumstatin45-132融合蛋白的表达载体，预测接头的合理性和可行性．并对融合蛋白活性进行验证。方法 采用基因融合序列重叠延伸（s0E）策略，构建新型TNFα-Tumstatin45-132表达载体；应用核酸和蛋白质序列软件Antheprot和PepTool对融合基因及接头部位翻译后在二级结构水平上的柔性、抗原性、亲水性等生物特性加以预测；用细胞毒试验和内皮细胞增殖试验测定表达的融合蛋白活性。结果 构建新型重组TNFα-Tumstatin45-132融合基因．经DNA测序证实与设计完全一致；TNFa-Tumstatinml32融合基因的氨基酸序列经软件分析，并与TNFα和Tumstatin45-132单独分析的结果比较，未出现新的抗原性，亲水性没有改变，接头部位具有很低的抗原性，接头部位呈中性；表达的融合蛋白经证实具有杀伤L929细胞和抑制ECV304细胞增殖的作用。结论 通过计算机软件对TNFα-Tumstatin45-132融合蛋白的预测，并与TNFα和Tumstatin45-132分别对比分析，有利于合理设计融合蛋白，最大程度地保留TNFα和Tumstatin45-132各自的生物活性和功能；生物活性分析证实设计具有一定的科学性和合理性。     

冠心病病人A型行为研究进展

从A型行为对冠心病的影响、冠心病病人A型行为的评估、心理干预3方面对冠心病病人A型行为的研究进展进行综述。     

坤泰胶囊联合来曲唑治疗多囊卵巢综合征不孕症的效果

〔摘 要〕 目的：探讨多囊卵巢综合征不孕症患者采用坤泰胶囊联合来曲唑治疗的临床效果。 方法：对重庆市万州区妇 幼保健院 2019 年 1 月至 2020 年 6 月期间收治的 60 例多囊卵巢综合征不孕症患者进行分组,以随机数字表法分成对照组与 观察组,各 30 例,观察组患者采用坤泰胶囊联合来曲唑治疗,对照组患者采用来曲唑治疗,比较两组患者治疗前后性激素 水平、卵巢卵泡质量、子宫内膜厚度、排卵率、妊娠率。 结果：治疗后,观察组患者的睾酮、雌二醇、促卵泡激素、促黄 体生成素水平均低于对照组,差异具有统计学意义（P ＜ 0.05）。观察组患者的直径＞ 18 mm 成熟卵泡数多于对照组,子 宫内膜厚度大于对照组,差异具有统计学意义（P ＜ 0.05）。治疗 50 d 后,观察组患者排卵率、妊娠率均高于对照组患者, 差异具有统计学意义（P ＜ 0.05）。 结论：对多囊卵巢综合征不孕症患者采用坤泰胶囊联合来曲唑治疗的效果显著,可以 有效改善患者性激素水平及其卵巢卵泡质量,增加子宫内膜厚度,提高妊娠率。     

异丙酚对大鼠内侧膝状体腹侧核团持续钠电流的影响

目的:研究异丙酚对大鼠内侧膝状体腹侧核团(MGBv)持续钠电流的影响. 方法: 42只SD大鼠,随机分为7组:0.9%氯化钠组(对照组)、5.6 μmolL-1、16.8 μmolL-1、56 μmolL-1、168 μmolL-1、560 μmolL-1异丙酚组和脂肪乳剂组(异丙酚溶媒组).采用脑片膜片钳技术,观察给药前后大鼠MGBv内持续钠电流的变化. 结果:6 μmolL-1异丙酚、0.9%氯化钠组(对照组)和10%脂肪乳剂(异丙酚溶媒组)对持续钠电流无影响(P<0.05),16.8 μmolL-1、 56 μmolL-1、 168 μmolL-1、560 μmolL-1异丙酚对持续钠电流有一定的的抑制作用.结论:5.6、16.8、56、168、560和1 800 μmolL-1异丙酚对大鼠MGBv内持续钠电流产生浓度依赖性抑制作用.     

对一种强化碱性戊二醛消毒剂的毒理学评价

为了解四环牌强化碱性戊二醛的安全性,进行了急性经口毒性、蓄积毒性、皮肤变态反应、小鼠骨髓嗜多染红细胞微核和精子畸形试验。结果,该剂对小鼠经口LD50为6329mg/kg（体重）,蓄积系数K>5;豚鼠皮肤变态反应试验未出现过敏反应;试验用戊二醛剂量诱导PCE微核细胞率与阴性对照组差异无统计学意义（P>0.05）,对小鼠精子畸形发生率无影响。因此,在本实验条件下,强化碱性戊二醛为实际无毒、弱蓄积毒性。极轻致敏性物质,没有致突变作用。     

阴囊湿疹样癌的围手术期护理

我院2002年1月-2005年12月采用自体大隐静脉移植显微外科修复治疗19例闭合性胭动脉损伤患者，术后通过早期细致的观察与护理，及时采取有效的措施，取得了满意效果，现报道如下。     

1例心脏原发嗜铬细胞瘤根治术的手术配合

原发心脏肿瘤的发病率很低，其部位、大小及活动度决定了临床表现。体外循环心脏停搏下手术切除是治疗心脏肿瘤最有效的方法。而嗜铬细胞瘤的病例异位于胸腔的病例不到1％，大多见于后纵隔，心脏原发嗜铬细胞瘤更少见。我院2007年3月收治1例，并于2007年4月行体外循环下嗜铬细胞瘤切除，取得了良好的效果。现将手术配合体会总结如下。     

Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats

The mitochondrial calcium uniporter (MCU) transports Ca²⁺ from the cytoplasm to the mitochondrial matrix and thus maintains Ca²⁺ homeostasis. Previous studies have reported that inhibition of MCU by ruthenium red (RR) protects the brain from ischemia/reperfusion (I/R) injury and that mitochondrial fission plays an important role in I/R injury. However, it is still not known whether MCU affects mitochondrial fission. In the present study, treatment with RR was found to decrease the concentration of free calcium in the mitochondria, calcineurin enzyme activity and dynamin-related protein 1 expression, and treatment with spermine was found to have the opposite effect in organisms subjected to occlusion of the middle cerebral artery lasting 2 h followed by 24 h reperfusion. These results indicate that MCU may be related to mitochondrial fission via modulating mitochondrial Ca²⁺ uptake and this relationship between MCU and mitochondrial fission may protect the brain from I/R injury.     

Clinical Outcomes in Patients with Non-Diagnostic Thyroid Fine Needle Aspiration Cytology: Usefulness of the Thyroid Core Needle Biopsy

Background

Patients with non-diagnostic thyroid fine needle aspiration cytology (FNAC) results undergo repeat FNAC or core needle biopsy (CNB) for definite diagnosis or surgical resection, or are followed up by clinical and ultrasound surveillance. We aimed at evaluating the risk of malignancy in patients with non-diagnostic FNACs and their clinical outcomes according to the follow-up modality.

Methods

We retrospectively reviewed 1,496 (8.8 %) cases with a non-diagnostic result on a first aspiration among 17,045 thyroid FNACs performed between October 2008 and August 2012. Of the non-diagnostic FNACs, 389 patients underwent a second FNAC; 125, CNB; and 89, thyroidectomy by clinical indication. The remaining patients were clinically followed up.

Results

The rate of a second non-diagnostic result was significantly higher on repeat FNAC than on CNB (33.2 vs. 2.4 %; p < 0.001). There was no significant difference in the malignancy risk among patients initially non-diagnostic, twice non-diagnostic, and thrice or more non-diagnostic, nor did this differ from the rate following CNB. No further malignancy was found in cases with ≥2 non-diagnostic CNBs. The malignancy risk was 51 % in those who underwent thyroidectomy. The sensitivity for detecting malignancy was 65 and 70 % for repeat FNACs and first CNBs, respectively, with no false positives seen in either test.

Conclusions

Approximately one-third of repeat FNACs after an initial non-diagnostic aspirate are non-diagnostic on repeat examination, and the malignancy risk may not reduce following repetitively non-diagnostic FNACs. However, a single CNB may be enough to exclude malignancy risk for patients with a non-diagnostic aspirate.