全文获取类型
收费全文 | 4420篇 |
免费 | 392篇 |
国内免费 | 32篇 |
专业分类
耳鼻咽喉 | 15篇 |
儿科学 | 130篇 |
妇产科学 | 62篇 |
基础医学 | 700篇 |
口腔科学 | 115篇 |
临床医学 | 532篇 |
内科学 | 858篇 |
皮肤病学 | 187篇 |
神经病学 | 127篇 |
特种医学 | 342篇 |
外科学 | 667篇 |
综合类 | 106篇 |
一般理论 | 3篇 |
预防医学 | 389篇 |
眼科学 | 93篇 |
药学 | 373篇 |
中国医学 | 3篇 |
肿瘤学 | 142篇 |
出版年
2021年 | 46篇 |
2015年 | 55篇 |
2014年 | 68篇 |
2013年 | 132篇 |
2012年 | 120篇 |
2011年 | 85篇 |
2010年 | 81篇 |
2009年 | 78篇 |
2008年 | 82篇 |
2007年 | 131篇 |
2006年 | 134篇 |
2005年 | 102篇 |
2004年 | 88篇 |
2003年 | 104篇 |
2002年 | 95篇 |
2001年 | 105篇 |
2000年 | 87篇 |
1999年 | 86篇 |
1998年 | 104篇 |
1997年 | 110篇 |
1996年 | 129篇 |
1995年 | 79篇 |
1994年 | 96篇 |
1993年 | 85篇 |
1992年 | 109篇 |
1991年 | 98篇 |
1990年 | 96篇 |
1989年 | 126篇 |
1988年 | 111篇 |
1987年 | 112篇 |
1986年 | 100篇 |
1985年 | 81篇 |
1984年 | 108篇 |
1983年 | 58篇 |
1982年 | 61篇 |
1981年 | 65篇 |
1980年 | 87篇 |
1979年 | 75篇 |
1978年 | 73篇 |
1977年 | 63篇 |
1976年 | 61篇 |
1975年 | 73篇 |
1972年 | 61篇 |
1970年 | 56篇 |
1969年 | 48篇 |
1965年 | 60篇 |
1964年 | 58篇 |
1963年 | 53篇 |
1962年 | 46篇 |
1960年 | 68篇 |
排序方式: 共有4844条查询结果,搜索用时 312 毫秒
61.
62.
The Charcot-Mane-Tooth disease type 1A (CMTlA) phenotype is most often associated with a 1.5 megabase (mb), tandem duplication of chromosome 17 band p12 (17˜12). The prevailing hypothesis is that the demyelinating neuropathy results from a dosage effect of the peripheral myelin protein gene PMP22 which is included within this duplication. We present a patient with clinical and electrophysiological features ofCMTlA in whom an extra PMP22 gene resulted from a rare unbalanced translocation of 17p to the X chromosome. This finding further supports the hypothesis of gene dosage as the basis for CMTlA. More-over, this case highlights the importance of fluorescence in siiu hybridization (FISH) as an alternative molecular technique in the diagnosis of CMTlA. 相似文献
63.
Inhibition of the Mixed Lymphocyte Culture Response by Antibody Following Successful Human Renal Transplantation 总被引:7,自引:0,他引:7
Immunoglobulin G, appearing after several months in the serum of a recipient of a successful kidney transplant from a closely matched sibling donor, was demonstrated to progressively inhibit unidirectional mixed lymphocyte cultures when donor lymphocytes were used either in responding or stimulating cell populations. The active recipient IgG had no effect in cultures in which donor cells were not used, nor did IgG obtained from other individuals show nonspecific inhibitory effects on cultures containing donor cells. It is suggested that the MLC inhibitory immunoglobulin may serve an immunoregulatory function after renal transplantation. 相似文献
64.
Detection of Legionella pneumophila using a real-time PCR hybridization assay 总被引:5,自引:0,他引:5
下载免费PDF全文
![点击此处可从《Journal of clinical microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Ballard AL Fry NK Chan L Surman SB Lee JV Harrison TG Towner KJ 《Journal of clinical microbiology》2000,38(11):4215-4218
A real-time PCR hybridization assay for Legionella pneumophila is described; the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water sample. The assay, including DNA extraction and confirmation of product identity, is completed within 90 min of receipt of a sample. 相似文献
65.
Summary Replication of a CELO large plaque (LP) mutant and that of its wild type small plaque (SP) parent was studied in the chorioallantoic membrane (CAM) and in the amnion of 11-day-old embryos. Although both strains produced essentially the same amount of virus in the tissue fluids, they differed in their rates of replication. Replication of the SP parent was maximal in the CAM 24 to 48 hours before that of the LP mutant. Whereas inclusions were observed in SP inoculated CAM 48 hours PI and were present during the course of study; LP inclusions were rare at 72 hours PI and thereafter; LP inclusions were seen at 72 hours PI. Fewer SP than LP particles were required to produce inclusions. No inclusions were seen in sections of the trachea and liver removed at 96 hours PI from embryos inoculated via the amniotic sac with LP and SP virus.Contribution 1466 of the Rhode Island Agriculture Experimental Station. 相似文献
66.
67.
Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila 总被引:10,自引:0,他引:10
下载免费PDF全文
![点击此处可从《Journal of clinical microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Gaia V Fry NK Afshar B Lück PC Meugnier H Etienne J Peduzzi R Harrison TG 《Journal of clinical microbiology》2005,43(5):2047-2052
A previously described sequence-based epidemiological typing method for clinical and environmental isolates of Legionella pneumophila serogroup 1 was extended by the investigation of three additional gene targets and modification of one of the previous targets. Excellent typeability, reproducibility, and epidemiological concordance were determined for isolates belonging to both serogroup 1 and the other serogroups investigated. Gene fragments were amplified from genomic DNA, and PCR amplicons were sequenced by using forward and reverse primers. Consensus sequences are entered into an online database, which allows the assignment of individual allele numbers. The resulting sequence-based type or allelic profile comprises a string of the individual allele numbers separated by commas, e.g., 1,4,3,1,1,1, in a predetermined order, i.e., flaA, pilE, asd, mip, mompS, and proA. The index of discrimination (D) obtained with these six loci was calculated following analysis of a panel of 79 unrelated clinical isolates. A D value of > 0.94 was obtained, and this value appears to be sufficient for use in the epidemiological investigation of outbreaks caused by L. pneumophila. The D value rose to 0.98 when the results of the analysis were combined with those of monoclonal antibody subgrouping. Sequence-based typing of L. pneumophila is epidemiologically concordant and discriminatory, and the data are easily transportable. This consensus method will assist in the epidemiological investigation of L. pneumophila infections, especially travel-associated cases, by which it will allow a rapid comparison of isolates obtained in more than one country. 相似文献
68.
Soluble IL-2 receptor and CD25 cells in psoriasis: effects of cyclosporin A and PUVA therapy. 总被引:3,自引:0,他引:3
下载免费PDF全文
![点击此处可从《Clinical and experimental immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
J I Duncan C Horrocks A D Ormerod A V Powles P H Whiting L Fry A W Thomson 《Clinical and experimental immunology》1991,85(2):293-296
A study was conducted to quantify soluble IL-2 receptor (sIL-2R) levels in sera of 57 chronic plaque psoriasis patients and correlate these measurements with disease activity and the number of IL-2R-positive (CD25+) lymphocytes in lesional biopsies of 11 cyclosporin A (CsA) and 13 psoralen plus ultraviolet radiation (PUVA) treated patients. Levels of sIL-2R showed a strong correlation with the psoriasis area and severity index (PASI). CsA and PUVA significantly reduced the PASI and sIL-2R levels to a similar degree after 4 weeks of treatment. Although the majority of CsA-treated patients who were biopsied showed reductions in lesional CD25+ cells, these did not reach statistical significance; in five patients biopsied who had PUVA treatment, no consistent effect on the numbers of CD25+ cells was observed. A significant correlation was found between CD25+ cells in lesional biopsies and the PASI score. 相似文献
69.
70.
J. A. D. Anderson Carole Buck Kate Danaher John Fry 《The British journal of general practice》1977,27(176):155-159
Sixty patients who visited their general practitioner were matched with 60 patients registered with the same doctor, who were of the same sex and in the same ten-year age group, and who had not visited the doctor for at least one year, but had recently experienced symptoms similar to those presented by the attending patients.
Comparison of the 60 pairs revealed the following differences, all substantial although not all statistically significant. The patients who visited the doctor perceived themselves as less healthy, fewer had attempted self-treatment, more reported serious personal problems, and fewer reported obstacles to visiting the doctor.
Differences between the pairs were negligible for total number of current ailments, effectiveness of self-treatment, if used, optimism about the healing powers of doctors, and fear of troubling their doctor with trivia.
相似文献