全文获取类型
收费全文 | 16700篇 |
免费 | 931篇 |
国内免费 | 39篇 |
专业分类
耳鼻咽喉 | 231篇 |
儿科学 | 697篇 |
妇产科学 | 254篇 |
基础医学 | 1920篇 |
口腔科学 | 274篇 |
临床医学 | 1166篇 |
内科学 | 4640篇 |
皮肤病学 | 634篇 |
神经病学 | 1327篇 |
特种医学 | 303篇 |
外科学 | 2613篇 |
综合类 | 66篇 |
一般理论 | 4篇 |
预防医学 | 1331篇 |
眼科学 | 430篇 |
药学 | 962篇 |
中国医学 | 45篇 |
肿瘤学 | 773篇 |
出版年
2023年 | 98篇 |
2022年 | 46篇 |
2021年 | 346篇 |
2020年 | 148篇 |
2019年 | 399篇 |
2018年 | 670篇 |
2017年 | 386篇 |
2016年 | 352篇 |
2015年 | 386篇 |
2014年 | 405篇 |
2013年 | 617篇 |
2012年 | 1087篇 |
2011年 | 1158篇 |
2010年 | 641篇 |
2009年 | 462篇 |
2008年 | 972篇 |
2007年 | 1024篇 |
2006年 | 1031篇 |
2005年 | 1049篇 |
2004年 | 909篇 |
2003年 | 967篇 |
2002年 | 880篇 |
2001年 | 633篇 |
2000年 | 785篇 |
1999年 | 578篇 |
1998年 | 76篇 |
1997年 | 47篇 |
1996年 | 56篇 |
1995年 | 46篇 |
1994年 | 42篇 |
1993年 | 28篇 |
1992年 | 197篇 |
1991年 | 178篇 |
1990年 | 155篇 |
1989年 | 108篇 |
1988年 | 106篇 |
1987年 | 94篇 |
1986年 | 79篇 |
1985年 | 83篇 |
1984年 | 33篇 |
1983年 | 36篇 |
1981年 | 14篇 |
1979年 | 26篇 |
1978年 | 16篇 |
1974年 | 15篇 |
1973年 | 19篇 |
1972年 | 14篇 |
1970年 | 20篇 |
1969年 | 16篇 |
1968年 | 23篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
INK4a/ARF locus alterations in human non-Hodgkin's lymphomas mainly occur in tumors with wild-type p53 gene 总被引:3,自引:0,他引:3
下载免费PDF全文
![点击此处可从《The American journal of pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Pinyol M Hernández L Martínez A Cobo F Hernández S Beà S López-Guillermo A Nayach I Palacín A Nadal A Fernández PL Montserrat E Cardesa A Campo E 《The American journal of pathology》2000,156(6):1987-1996
INK4a/ARF locus codes for two different proteins, p16(INK4a) and p14(ARF), involved in cell cycle regulation. p14(ARF) is considered an upstream regulator of p53 function. To determine the role of these genes in the pathogenesis of human non-Hodgkin's lymphomas we have analyzed exon 1beta, 1alpha, and 2 of the INK4a/ARF locus and p53 gene aberrations in 97 tumors previously characterized for p16(INK4a) alterations. p53 alterations were detected in four of 51 (8%) indolent lymphomas but in 15 of 46 (33%) aggressive tumors. Inactivation of p14(ARF) was always associated with p16(INK4a) alterations. Exon 1beta was concomitantly deleted with exon 1alpha and 2 in eight tumors. One additional lymphoblastic lymphoma showed deletion of exon 1alpha and 2 but retained exon 1beta. No mutations were detected in exon 1alpha and 1beta in any case. Two of the three mutations detected in exon 2 caused a nonsense mutation in the p16(INK4a) reading frame and a missense mutation in the ARF reading frame involving the nucleolar transport domain of the protein. The third mutation was a missense mutation in the p16(INK4a) reading frame, but it was outside the coding region of p14(ARF). Aggressive lymphomas with p14(ARF) inactivation and p53 wild type showed a significantly lower p53 protein expression than tumors with no alteration in any of these genes. In this series of tumors, inactivation of the INK4a/ARF locus mainly occurred in tumors with a wild-type p53 gene because only two lymphomas showed simultaneous aberrations in these genes. Tumors with concomitant alterations of p16(INK4a) and p14(ARF)/p53 genes seem to exhibit a worse clinical behavior than lymphomas with no alterations or isolated inactivation of any of these genes. These findings indicate that p14(ARF) genetic alterations occur in a subset of aggressive NHLs, but they are always associated with p16(INK4a) aberrations. Concomitant disruption of p16(INK4a) and p14(ARF)/p53 regulatory pathways may have a cooperative effect in the progression of these tumors. 相似文献
72.
Summary The phenotypically wild strain I3 of Chlamydomonas reinhardtii, carrying a cryptic mutation at the nit-6 locus, was distinguished from strains 21gr (cryptic mutant at nit-5) and 6145c (wild type) because of the ability of I3 to grow on nitrate media containing 2mM tungstate.Molybdopterin-cofactor (MoCo) mutants 102 (double mutant at nit-5 and nit-6) and 104 (mutant at nit-4) grew on nitrate media supplemented with high concentrations of molybdate, although final cell densities were 40–60% lower and generation times 3.5 to six fold longer than for wild type. Under these conditions, nitrate reductase (NR) activity of the mutants, when measured either in situ or in vitro, was practically undetectable. The MoCo defective mutant 307 (nit-3) was not molybdate repairable. Although NR activity was not restored in vitro by molybdate in any of the MoCo– mutant strains, their extracts had free NR-diaphorase subunits together with NR-subunits assembled into high molecular weight species.Our results indicate that: a) nit-4, nit-5 and nit-6 loci are responsible for molybdate processing in the cell; b) nit-3 may encode a component of the pterin moiety biosynthetic route; c) NR subunits can assemble in the presence of an inactive MoCo; d) high concentrations of molybdate can replace partially in vivo but not in vitro the function of nit-4 and the combined function(s) of the nit-5 and nit-6 gene products. 相似文献
73.
Alberto Utrero-Rico Javier Ruiz-Hornillos Cecilia González-Cuadrado Claudia Geraldine Rita Berta Almoguera Pablo Minguez Antonio Herrero-González Mario Fernández-Ruiz Octavio Carretero Juan Carlos Taracido-Fernández Rosario López-Rodriguez Marta Corton José María Aguado Luisa María Villar Carmen Ayuso-García Estela Paz-Artal Rocio Laguna-Goya 《The Journal of allergy and clinical immunology》2021,147(5):1652-1661.e1
74.
Carreiro-Rodríguez M Hernández-Blanca D Fernández LM Montes de Oca I Castillo E Sánchez JL 《Investigación clínica》1999,40(4):267-276
We describe a case of hypertrophic pulmonary osteoarthropathy (HPOA) in an adult patient with acquired immunodeficiency syndrome (AIDS). This is the ninth case of HPOA associated with AIDS in adults, reported in the literature. The presence of pulmonary tuberculosis was also suspected, based on clinical grounds. Cases of clubbing associated with AIDS infection are reviewed. 相似文献
75.
Cristina Benito Eloína González-Mancebo M Dolores Alonso Díaz de Durana Rosa M Tolón Montserrat Fernández-Rivas 《Annals of allergy, asthma & immunology》2007,98(6):580-584
BACKGROUND: Coconut (Cocos nucifera) is a monocotyledonous plant of the Arecaceae family. Allergy to coconut is infrequent, with only 5 cases reported so far in the medical literature. OBJECTIVE: To identify coconut allergens in 2 patients allergic to this food. METHODS: We describe 2 patients allergic to coconut: an adult pollen-allergic patient monosensitized to coconut who presented with severe oropharyngeal symptoms and a child with a previous allergy to walnut, not allergic to pollen, who developed anaphylaxis on coconut ingestion. Both patients had positive skin prick test results and serum specific IgE (CAP) to coconut. IgE sodium dodecyl sulfate-polyacrylamide gel electrophoresis immunoblotting was performed to identify the allergens involved, and a strong IgE binding band detected in both patients was further analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS). Stability to pepsin digestion of the coconut extract and its cross-reactivity with tree nuts were studied. RESULTS: An immunoblot showed an almost identical profile of IgE binding proteins in the coconut extract in both patients who reacted strongly to a band of approximately 29 kDa. The peptide analysis by MALDI-TOF MS of this band obtained the sequence GHGKREDPEKR. The protein with the highest correlation with this peptide was found to be a 7S globulin from Elaeis guineensis, another oil palm species also belonging to the Arecaceae family. The 29-kDa band was digested by pepsin in less than 1 minute. Cross-reactivity among coconut, walnut, and hazelnut was demonstrated by CAP inhibition in patient 2. CONCLUSION: We have identified a 7S storage protein as a novel coconut allergen. 相似文献
76.
Concepción Núñez Diana Alecsandru Jezabel Varadé Isabel Polanco Carlos Maluenda Miguel Fernández-Arquero Emilio G de la Concha Elena Urcelay Alfonso Martínez 《BMC medical genetics》2006,7(1):32-5
Background
Celiac disease (CD) is a chronic disorder characterized by a pathological inflammatory response after exposure to gluten in genetically susceptible individuals. The HLA complex accounts for less than half of the genetic component of the disease, and additional genes must be implicated. Interleukin-10 (IL-10) is an important regulator of mucosal immunity, and several reports have described alterations of IL-10 levels in celiac patients. The IL-10 gene is located on chromosome 1, and its promoter carries several single nucleotide polymorphisms (SNPs) and microsatellites which have been associated to production levels. Our aim was to study the role of those polymorphisms in susceptibility to CD in our population. 相似文献77.
Development of DNA vaccines against hemolytic-uremic syndrome in a murine model 总被引:2,自引:0,他引:2
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Capozzo AV Pistone Creydt V Dran G Fernández G Gómez S Bentancor LV Rubel C Ibarra C Isturiz M Palermo MS 《Infection and immunity》2003,71(7):3971-3978
Shiga toxin type 2 (Stx2) produced by Escherichia coli O:157H7 can cause hemolytic-uremic syndrome in children, a disease for which there is neither a vaccine nor an effective treatment. This toxin consists of an enzymatically active A subunit and a pentameric B subunit responsible for the toxin binding to host cells, and also found to be immunogenic in rabbits. In this study we developed eukaryotic plasmids expressing the B subunit gene of Stx2 (pStx2B) and the B subunit plus the gene coding for the A subunit with an active-site deletion (pStx2 Delta A). Transfection of eukaryotic cells with these plasmids produced proteins of the expected molecular weight which reacted with specific monoclonal antibodies. Newborn and adult BALB/c mice immunized with two intramuscular injections of each plasmid, either alone or together with the same vector expressing the granulocyte and monocyte colony-stimulating factor (pGM-CSF), elicited a specific Th1-biased humoral response. The effect of pGM-CSF as an adjuvant plasmid was particularly notable in newborn mice and in pStx2B-vaccinated adult mice. Stx2-neutralizing activity, evaluated in vitro on VERO cell monolayers, correlated with in vivo protection. This is the first report using plasmids to induce a neutralizing humoral immune response against the Stx2. 相似文献
78.
79.