普罗布考对体外培养的主动脉平滑肌细胞NF—kB活性的影响

目的：观察普罗布考（probucol)对血管平滑肌细胞（VSMCs)核因子kB(NF-kB)活性的调控作用,以探讨probucol在动脉粥样硬化（AS）和经皮腔内冠状动脉成形术后再狭窄（RS）防治中的某些可能的作用机制。方法：采用电泳迁移位移测定法观察probucol对VSMCsNF-kB活性的影响。结果：H2O2或胎牛血清（NCS）处理VSMCs72h后,NF-kB活性均明显增强;而加入100umol/Lprobucol后,NF-kB活性受到部分抑制,抑制率分别为37.1%和14.8%。结论：Probucol抑制NCS、H2O2诱导的VSMCsNF-kB的活性,可能是其临床有效防治RS及AS的作用机制之一。     

人工破膜对诊断羊水过少的价值

目的 :探讨人工破膜在诊断羊水过少中的意义。方法 :观察组是根据人工破膜时所流出的羊水量判断羊水多少 ;对照组则用同期产前超声检查所见判断羊水多少。以产时所见羊水量作为最终诊断。结果 :人工破膜和超声诊断羊水过少的符合率分别是 79 73 %和 82 76 % ;两者无显著差异 (P >0 0 5 ) ;人工破膜漏诊率 2 84% ,超声是 6 15 % ,两者无显著差异 (P >0 0 5 )。结论 :人工破膜是诊断本病的一种有价值的方法 ,结合超声检查可作为诊断本病的依据     

数字减影泪道造影术的应用

目的：评价数字减影泪道造影术在临床检查泪道阻塞中的应用价值。方法：利用数字减影泪道造影术对82只正常眼及56只溢泪眼进行检查。并对图像进行分析。结果：数字减影泪道造影术能够提供清晰的泪道图像并对泪道阻塞的部位及泪道改变作出准确的判断。结论：数字减影泪道造影术是一种快速、有效和无损伤的检查方法。在泪道阻塞的诊断和治疗中将起重要作用。     

孤立性蝶窦病变的诊断与治疗

目的;提高孤立性蝶窦病变的诊断和治疗率。方法;总结使用鼻窦内窥镜诊治孤立性蝶窦病变１２例的全过程。结果：１２例孤立性蝶窦病变的患者的均获准确诊断,使用鼻窦镜行经鼻腔蝶窦手术,有效率为８３．３％,随访６￣２４个月,１０例无复发,复发２例二次手术后治愈;结论：孤立性蝶窦病变患者可用鼻窦内窥镜诊断与治疗。     

血管运动性鼻炎激光治疗鼻腔粘膜致敏区的筛选

目的 :随访和对比激光治疗血管运动性鼻炎鼻腔粘膜不同区域的致敏性 ,以明确最佳治疗点。方法 :随机选择确诊的血管运动性鼻炎 1 0 3例 ,分为下鼻甲、中鼻甲、中鼻甲前 1 /3段 3组进行激光治疗。结果 :下鼻甲组总有效率为 66.7% ,中鼻甲组为 97.1 % ,中鼻甲前 1 /3段为 94.4% ,两中鼻甲组与下鼻甲组总有效率差异有显著性 (P<0 .0 0 5) ,中鼻甲组与中鼻甲前 1 /3段组总有效率差异无显著性 (P>0 .0 5)。结论 :中鼻甲前 1 /3段是血管运动性鼻炎患者鼻粘膜最敏感的区域 ,是最佳激光治疗点。     

足底板对类风湿性关节炎患者足底压的影响

目的 :测定类风湿性关节炎病人足底压 ,评价足底板的生物力学效应。方法 :12名女性类风湿性关节炎病人和 8名健康女性进行年龄和体重匹配。用 F- Scan系统进行动态足底压测量 ,Kistler床反力平台用以校正测量精确性。测量足底峰压和垂直分力 ,评价足底板的生物力学表现。结果 :类风湿性关节炎病人中足底压明显高于健康人。使用足底板后 ,足底总的峰压明显降低 ,前足、后足峰压减低 ,中足峰压增高。而垂直分力改变不大。结论 :足底板能显著降低足底压力 ,使足底压力重分布 ,减轻类风湿足痛。特殊设计的足底板对类风湿足痛的治疗能起重要的作用     

Effects of arotinoid acid on induction of apoptosis and differentiation and telomerase activity and cell cycle in the HL～60 cell line

Objective To investigate the effects of arotinoid acid (Ro13-7410) on the morphological and functional alterations of leukemia HL-60 cell line and compared with those of RA.Methods Differentiation of HL-60 cells was assessed by morphology and by NBT reduction.Trypan blue exclusion was used to determine viability.Apoptosis was assessed by changes in cell morphology and by measurement of fragmented DNA using the PCD-assay-kit.Telomerase PCR ELISA-kit tested telomerase activity.The cell cycle was analyzed by flow cytometry.Results Incubation of the HL-60 cells with 10(-6)-10(-8) mol/L Ro13-7410 resulted in suppression of cell growth.Apoptotic cells were detected following exposure to 10(-6) mol/LRo13-7410 for 3 hours by measurement of the 'in situ' enzymatic labeling of DNA breaks with biotinylated dUTP.Ultrastructural examination of Ro13-7410-treated samples showed cells with chromatin compaction and cytoplasm condensation and the presence of 'apoptotic bodies'.Cells induced into apoptosis were accompanied by increase of intracellular free Ca[2+].Percentage of HL-60 cells reduced NBT following incubation with Ro13-7410 was lower than with all-trans retinoic acid (RA) (27% vas 85%).Telomerase PCR-ELISA assay showed that HL-60 cells cultured in the absence of inducing agents had significant telomerase activity.Telomerase activity declined gradually after 10(-6) mol/L Ro13-7410 treatment, and changes becoming evident at 1 day.The inhibition of telomerase activity at day 5 of treatment with Ro13-7410 was less effective than with RA.DNA flow cytofluorimetric analysis revealed that Ro13-7410 caused partial cell arrest in the G(2)/M phase after a 2-day treatment and the percentage of cells arrested in the G(2)/M phase increased after 4-days treatment.With RA-treated cells, a reduction in the percentage of cells in the G(2)/M phase was observed after 2-day of treatment.Conclusion Our study shows that Ro13-7410 suppresses HL-60 cells growth mainly via the induction of apoptosis and is less effective than RA in induction differentiation.Ro13-7410 dramatically inhibits telomerase activity during the course of induction and results in G(2)/M arrest within 2 days.These findings suggest that Ro13-7410 is worthy of further study for its effects on leukemic cells.     

定量PCR检测缺失型DMD/BMD携带者的研究

研究DMD/BMD携带者临床诊断的有效手段。方法 :运用双重定量PCR及剂量系数分析 ,检测 2 6例正常对照 ,7例肯定携带者和 2 1例可疑携带者 ,部分结果与短片段重复顺序多态性方法及CK值作了比较。结果 :确定了判定参考标准 ,可疑携带者中 ,患儿母亲检测阳性率为 5 7.9% ,8例经短串联重复顺序多态性分析 ,得到了完全验证。结论 :定量PCR检测DMD/BMD携带者快速、敏感、准确 ,可在临床诊断中应用。     

运用原位DNA末端标记法研究细胞凋亡对乳腺癌预后的影响

研究临床乳腺癌标本中细胞凋亡的发生情况,评价其在预测后在的意义。方法搜集９１例浸润性乳腺癌的石蜡组织切片,运用原位ＤＮＡ断裂位点的末端标记法,检测细胞凋亡,计算凋亡细胞占肿瘤细胞的百分比,求得凋亡指数。结果本组病例细胞凋亡发生率为９１．２％,凋亡指数分为两组：０～０．２１和０．２８～０．６２,凋亡指数高低与腋淋巴结转称相关（Ｐ＜０．０１）。生存率单因素分析中,凋亡指数高的病例,无病生存率（Ｐ＝０．００９５）及总生存率（Ｐ＝０．０３４８）均优于凋亡指数低的病例。但Ｃｏｘ模型多因素分析末能指示凋亡指数是一个独立的预后指标。结论在乳腺癌组织中,细胞凋亡是一种自发现象,其发生情况各有不同,研究初步提示了细胞凋亡在乳腺癌预后中的作用,了解了凋亡和腋淋巴结转移的相关性。