单纯缺血预处理对未成熟兔心脏的作用研究

目的 探讨缺血预处理（Ｉｓｃｈｅｍｉｃ Ｐｒｏｃｏｎｄｉｔｉｏｎｉｎｇ ＩＰＣ）对未成熟心脏全心缺血再灌注损伤的影响。方法 对经历５Ｍｉｎ缺血、１０Ｍｉｎ再灌注的幼兔（１４～２１天）心脏进行离体灌注,观察其在生理温度（３９℃）下接受３０Ｍｉｎ缺血、４０Ｍｉｎ再灌注的心肌酶释放、心肌能量及病理变化。结果 ＩＰＣ组全心缺血后心脏停跳持续时间显明延长（Ｐ〈０．０１）,心肌ＡＴＰ含量明显减少（Ｐ〈０．００     

甲硝唑磷酸酯钾的家兔药物动力学研究

:目的 :研究甲硝唑磷酸酯钾 (PMP)及甲硝唑 ((MTZ)在家兔血中的药物动力学参数。方法 :家兔iv和im等量的PMP、MTZ(10 0 μmol/kg)后 ,用HPLC苦味酸内标法测定家兔血浆中PMP和MTZ的浓度。 结果 :家兔iv等量PMP和MTZ后 ,其家兔血中PMP和MTZ药 -时曲线均符合二室开放模型 ,但其PMP释出的MTZ药 -时曲线较MTZ的药 -时曲线稍低 ,前者的AUC为后者的 47 4%。家兔im等量PMP和MTZ后 ,PMP释出的MTZ药 -时曲线为一室模型 ,MTZ的药 -时曲线为二室模型。前者的Cmax较后者低 ,其AUC为后者的38 9%。结论 :家兔ivPMP和MTZ后 ,二者末端的消除速率接近 ;家兔imPMP和MTZ后 ,二者的Tp 相近。     

天麻丸中天麻的显微定量研究

目的 :制定天麻在天麻丸中的显微定量标准。方法 :用显微定量法 ,以天麻的木化厚壁细胞为显微特征物 ,对天麻丸中的天麻进行测定。结果 :天麻含量与每mg特征数呈显著的线性正相关。回归方程为 y =6 2 35x 1 80× 10 -3 (γ =0 9999,a =0 0 5 )。结论 :显微定量法测定天麻丸中天麻的含量是可行的 ,结果可靠。     

477例患者血液透析中留置中心静脉导管的临床分析

目的：总结留置中心静脉导管的经验,探讨如何防治中心静脉留置导管的并发症。方法：分析１９９２年７月！￣１９９８年７月我院血液净化中心对４７７例肾功能不全患,留置中心静脉导管共５６６例次。     

ATP生物发光法检测卵巢癌细胞对化疗药物的敏感性

目的 探讨ＡＴＰ生物发光法药物敏感试验在卵巢癌中的应用的可行性。方法 以ＡＴＰ生物发光法检测卵巢癌ＨＯ－８９１０细胞对１１种常用化疗药物的敏感性,并与ＭＴＴ法相比较。结果 ＡＴＰ浓度、细胞浓度的对数与发光强度（ＲＬＵ）的对数值之间有较好的线性关系,并存在直线回归关系。两种方法测定的抑制率基本符合,但ＡＴＰ法测定的抑制率数值大,变异系数小,结果稳定。结论 ＡＴＰ生物发光法是一种高度敏感的化疗药物敏感     

重型颅脑损伤66例死因分析

回顾分析２３６例重型颅脑损伤患者中６６例死亡病人的临床资料,结果显示,受伤原因中车祸伤占４５．８％,堕落伤占２４．２％,跌伤占１８．１％,ＣＴ扫描见脑挫裂伤并颅内血肿占６３．６％,单纯脑挫裂伤和颅内血肿各占９．０％,２７．３％。采用亚低温加常规综合治疗后死亡率仅为１８．２％,而非亚低温加常规综合治疗死亡率为２９．２％。     

26例狼疮性肾炎并发急性肾功能衰竭的临床病理分析

为探讨狼疮性肾炎并发急性肾功能衰竭的危险因素,收集了符合狼疮性肾炎并发急性肾功能衰竭的病例２６例,与未发生急性肾功能衰竭的狼疮性肾炎３０例进行临床病理分析。结果：狼疮性肾炎并发急性肾功能衰竭常发生在具有临床活动指标及病理活动指标的患者,病理类型以Ⅳ型、Ⅴ型为主,其诱因有感染、不规则的激素治疗、劳累及日晒。结果表明：控制狼疮性肾炎的活动,避免诱发因素,是防止急性肾功能衰竭发生的重要环节。     

Optimal immobilization of penicillinase for lon-selective electrode

Penicillin sensor was prepared by immobilizing penicillinase (Pcase) on H⁺-selective carboxylated poly (vinyl chloride) (PVC-COOH) membrane or cellulose filter membrane. The immobilization techniques are as follows. Pcase was immobilized with GTH on H⁺-selective PVC-COOH membrane or some amount of BSA was dropped on that membrane. Another method to make immobilization is to mix type I Pcase with GTH and drop on a cellulose filter membrane. According to immobilization techniques, there were some differences in response properties of enzyme electrodes, however, all electrodes responded to Pcase-resistant penicillin derivatives. Pcase immobilized on cellulose filter membrane with H⁺-selective PVC membrane eletrode was more stable and more sensitive to penicillinase-resistant penicillin derivatives than any other immobilization techniques.     

Epimerization and Hydrolysis of Dalvastatin,a New Hydroxymethylglutaryl Coenzyme A (HMG-CoA) Reductase Inhibitor

In aqueous solutions, dalvastatin (1) undergoes epimerization as well as hydrolysis. The transformation of the drug was studied as a function of pH at 25°C in aqueous solutions containing 20% acetonitrile. At all pH values, first-order plots for the conversion are biphasic, indicating rapid equilibration of 1 with its epimer (2) and slower hydrolysis of 1 to the corresponding -hydroxy acid (3). Apparent first-order rate constants for the biexponential equation are given as a function of pH. The alkyl–oxygen cleavage of the lactone ring results in the epimerization of 1 to 2, whereas the acyl–oxygen cleavage results in the hydrolysis of 1 to 3. The epimerization is an S_N1 reaction reaching an equilibrium of [l] _eq/[2] _eq = 1.27. The epimerization rate is increased with an increase in the water content of the solvent. The hydrolysis of 1 to 3 is acid and base catalyzed. The hydrolysis is reversible in acidic media and irreversible in neutral and basic media. At pH values greater than 9, the hydrolysis reaction proceeds more rapidly than the epimerization.