Do Differences in Panton-Valentine Leukocidin Production among International Methicillin-Resistant Staphylococcus aureus Clones Affect Disease Presentation and Severity?

Panton-Valentine leukocidin (PVL) production by methicillin-resistant Staphylococcus aureus (MRSA) was determined in vitro using the enzyme-linked immunosorbent assay (ELISA), and associations with clinical presentation and bacterial genetic characteristics were examined. PVL production ranged from 0.02 to 4.865 μg/ml and correlated with a multilocus sequence type (MLST) clonal complex associated with specific PVL phage types. A relationship between PVL production and clinical presentation or patient demographics could not be demonstrated.     

Prior peritraumatic dissociative experiences affect autonomic reactivity during trauma recall

To better understand the psychophysiological correlates of peritraumatic dissociation (PD), the present study examined the relationship between reports of prior PD and sympathetic and parasympathetic functioning in response to a laboratory stress paradigm in 39 traumatized female undergraduates. Participants were asked to talk about their most distressing traumatizing experience while continuous measures of heart rate, pre-ejection period, and respiratory sinus arrhythmia were taken. Overall, high dissociators had significantly larger increases in heart rate and larger decreases in pre-ejection period and respiratory sinus arrhythmia during trauma recall. In opposition to our hypothesis, these results suggest that female trauma victims reporting high levels of PD during a prior trauma may be more reactive to traumatic reminders than victims who report lower levels of PD.     

Unusual and rare tumours of the liver

While adenoma, haemangioma, hepatocellular carcinoma and metastasis constitute the majority of tumours encountered in the liver, the liver may also be the primary site for a range of more unusual tumours, the diagnosis of which may not be possible without sampling for histology. This review highlights the clinical and morphological features of five such unusual tumours: calcifying nested stromal-epithelial tumour, solitary fibrous tumour, epithelioid haemangioendothelioma, inflammatory myofibroblastic tumour and PEComa, with particular emphasis on their distinction from the more common tumours of the liver.     

Systematic search for neutropenia should be part of the first screening in patients with poikiloderma

Poikiloderma occurs in a number of hereditary syndromes, the best known of which is Rothmund-Thomson syndrome (RTS). Differential diagnoses include Dyskeratosis Congenita (DC) with high genetic heterogeneity and Clericuzio-type Poikiloderma with Neutropenia (CPN) due to mutations in the C16orf57 gene. Mutations in the RECQL4 gene are only observed in two thirds of RTS patients. In this study, 10 patients referred for syndromic poikiloderma and negative for RECQL4 sequencing analysis were investigated for C16orf57 mutations. Two C16orf57 heterozygous nonsense mutations (p.W81X and p.Y89X) were identified in a 5-year-old female child presenting with generalized poikiloderma, dental dysplasia, gingivitis, nail dystrophy, palmoplantar keratoderma and pachyonychia of the great toenails. Previously undetected and silent neutropenia was evidenced after C16orf57 molecular analysis. Neutropenia was absent in the C16orf57-negative patients. This report confirms that neutrophil count should be performed in all patients with poikiloderma to target the C16orf57 gene sequencing analysis, prior to RECQL4 analysis.     

McGill Brisbane Symptom Score for patients with resectable pancreatic head adenocarcinoma

AIM: To evaluate the ability of the McGill Brisbane Symptom Score (MBSS) to predict survival in resectable pancreatic head adenocarcinoma (PHA) patients.METHODS: All PHA patients (n = 83) undergoing pancreaticoduodenectomy at the McGill University Health Center, Quebec between 1/2001-1/2010 were evaluated. Data related to patient and cancer characteristics, MBSS variables, and treatment were collected; univariable and multivariable survival analyses were performed. We obtained complete follow-up until February 2011 in all patients through the database of the provincial health insurance plan of Quebec. The unique health insurance numbers of these patients were used to retrieve information from this database which captures all billable clinical encounters, and ensures 100% actual survival data.RESULTS: Median survival was 23 mo overall: 45 mo for patients with low MBSS, 17 mo for high MBSS (P = 0.005). At twelve months survival was 83.3% (95%CI: 66.6-92.1) vs 58.1% (95%CI: 42.1-71.2) in those with low vs high MBSS, and24 mo survival was 63.8% (95%CI: 45.9-77.1) and 34.0% (95%CI: 20.2-48.2) respectively. In the multivariate Cox model (stratified by chemotherapy), after addition of clinically meaningful covariates, MBSS was the variable with the strongest association with survival (HR = 2.63; P = 0.001). Adjuvant chemotherapy interacted with MBSS category such that only high MBSS patients accrued a benefit. In univariate analysis we found a lower mortality in high MBSS but not low MBSS patients receiving adjuvant chemotherapy. This interaction variable, on Cox model, resulted in an adjusted mortality HR for the high MBSS (compared to low MBSS) of 4.14 (95%CI: 1.48-11.64) without chemotherapy and 2.11 (95%CI: 1.06-4.17) with chemotherapy.CONCLUSION: The MBSS is a simple prognostic tool for resectable PHA. Preoperative categorization of patients according to the MBSS allows effective stratification of patients to guide therapy.     

Midwives and Obstetrician-Gynecologists Collaborating for Native American Women's Health

Certified Nurse-Midwives (CNMs) and Obstetrician-Gynecologists (OBGs) have a long history of successful collaborative practice serving Native American women from the 1960s. CNMs provide holistic, patient-centered care focusing on normal pregnancy and childbirth. OBGs support CNMs with consultation services focusing on complications during pregnancy and specialty gynecology care. Collaborative care in Indian Health Service and Tribal sites optimizes maternity care in a supportive environment, achieving excellent outcomes including low rates of cesarean deliveries and high rates of successful vaginal birth after cesarean.     

Mullerian agenesis with vaginal vault prolapse following mechanically created neovagina

A 21-year-old with mullerian agenesis, presented with vaginal mass. Previously, she developed a neovagina by the use of vaginal dilators. Examination revealed vaginal vault prolapse. She was surgically treated with vaginal vault suspension using a mesh.