Effect of human osteopontin on proliferation, transmigration and expression of MMP-2 and MMP-9 in osteosarcoma cells

Background To explore the effect of human osteopontin (hOPN) on the proliferation, transmigration and expression of matrix metallproteinase-2 (MMP-2) and matrix metallproteinase-9 (MMP-9) in osteosarcoma (OS) cells in vitro.Methods The prokaryotic-expression vector of hOPN was produced, hOPN was then subcloned into E. coli BL21 (DE3) cells and purified with ProBond^TM Columns. The proliferation, cell cycle and the expression of cycUn A in OS cells were investigated by using MTT assay, flow cytometry and Western blot respectively. The transmigration of OS cells was checked by using transwell cell culture chamber.The micro-pore-filter-membrane system was used to study the chemiotaxis of hOPN to OS cells. The levels of total protein were examined according to Coomassie Brilliant Blue manuals. The expression of MMP-2 and MMP-9 were evaluated by detecting the volume of degradation of gelatin on SDS-PAGE gel.Results The prokaryotic-expression vector of hOPN and purified hOPN protein were achieved hOPN promoted OS cells proliferation in a dose-dependent manner, and stimulated cyclin A expression in OS cells to accelerate cell division cycle, hOPN facilitated the trans-membrane migration of OS cells.hOPN also enhanced the secretion of MMP-2 and MMP-9 in OS cells.Conclusion hOPN could stimulate cyclin A expression in OS cells, hOPN has chemiotaxis to OS cells and increases their transmigration, hOPN enhances the secretion of MMP-2 and MMP-9 in OS cells.     

高血压性脑梗塞血压与梗塞部位及范围的联系

报道了高血压脑梗塞患者190例,其中老年人134例,占70.5％。研究表明患者过去最高血压(平均动脉压18.0l±2.62kPa)与脑梗塞数目(2.47±1.18)和面积(1.37±0.89cm)之间呈显著正相关(分别γ=0.4180,γ=0.4086;p<0.01)。而入院时血压水平(平均动脉压15.02±2.10kpa)与其梗塞面呈显著负相关(γ=-0.2398,P70岁组与<60岁组,其梗塞数目与面积相关也显著(γ=0.3524,γ=0.3662,γ=0.3295;p<0.01)。梗塞以基底节区和侧脑部位最多,分别为43.41％(201/463个);23.33％(108/463个),均较其余两组有显著差别,(t=3.29,t=2.28;P<0.01)。脑腔隙性梗塞26.84％(51/190例),非腔隙型71.16％(139/190例);有症状者较无症状者多见。结果表明:过去最高血压与脑梗塞部位和范围关系密切,入院时血压水平可能是诱发脑梗塞的直接原因之一,各组梗塞数目与面积趋向一致;基底节区、侧脑是老年高血压性脑梗塞多发部位。     

全机器人心脏手术麻醉中体外循环对肺功能的影响

目的观察机器人心脏手术麻醉中,在单肺通气（OLV）和二氧化碳气胸的基础上,体外循环（CPB）对肺功能的影响。方法 98例行机器人心脏手术的患者,根据手术是否需要行体外循环分为两组,体外循环组（On-pump组）58例,非体外循环组（Off-pump组）40例。两组分别于麻醉诱导后双肺通气20 min（T1）、OLV 25 min（T2）、On-pump组体外循环后OLV 25 min或Off-pump组完成主要手术操作后OLV 20 min（T3）、手术结束前双肺通气20 min（T4）四个时间点,记录机械通气参数、气道峰压（Ppeak）,并抽取动脉血做血气分析,根据公式计算动态肺顺应性（Cdyn）、肺泡-动脉氧分压差（PA-aDO2）、动脉-肺泡氧分压比率（a/A）、氧合指数（OI）等指标。结果术中On-pump组T3比T2时间点呼气末二氧化氮分压（PETCO2）、Ppeak、二氧化碳分压（PaCO2）、PA-aDO2高,脉搏氧饱合度（SpO2）、Cdyn、氧分压（PaO2）、OI、a/A低（P〈0.01）;Off-pump组T3比T2时间点无明显变化（P〉0.05）;两组T3时刻组间比较,On-pump组比Off-pump组Ppeak、PaCO2、PA-aDO2高,SpO2、Cdyn、PaO2、OI、a/A低（P〈0.01）。结论在机器人心脏手术麻醉中,体外循环将使肺通气功能和换气功能明显降低,耐受OLV的能力下降。     

分期辨治糖尿病足的临床研究

采用中医分期辨治为主的综合疗法并与山莨菪碱(654-2)作对照,观察60例非胰岛素依赖型糖尿病足患者（治疗组30例,对照组30例）的临床疗效及血脂和载脂蛋白变化。结果治疗组可明显降低胆固醇（CHOL）、甘油三酯（TG）和升高高密度脂蛋白（HDL-C）,治疗前后相比具有非常显著性差异（P＜0.01）,载脂蛋白A     

Acupuncture Treatment for Multiple Aortitis ——A Clinical Report of 40 Cases

In order to evaluate the therapeutic effects of acupuncture-moxibustion on multiple aortitis, 80 cases （with involvement of the main arteries in the head and arms） were randomly divided into a treatment group treated with acupuncture-moxibustion, and a control group treated with TCM and western drugs. The therapeutic effects were compared. The total effective rate was 95% in the treatment group （including 15% of the cure rate and 62.6% of the marked relief rate）, and the total effective rate was 75% in the control group （including 12.5% of the marked relief rate）. There was a significant difference in the therapeutic effects （P〈0.01） between the two groups. It can be concluded that acupuncture-moxibustion is obviously superior to the routine drug therapy for treatment of multiple aortitis.     

超声复合微泡促进骨髓间充质干细胞归巢对大鼠前列腺炎的治疗作用

目的 研究静脉注射骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)对大鼠前列腺炎的治疗作用.方法 贴壁法分离、培养、扩增BMSCs.将50只SD大鼠按随机数字表法分为慢性细菌性前列腺炎(chronic bacterial prostatitis,CBP)组、CBP+BMSCs组、CBP+超声复合微泡(microbubble-enhanced therapeutic ultrasound,MEUS)组、CBP+ MEUS+ BMSCs组和正常对照组,每组10只.在小动物超声引导下向大鼠前列腺两侧叶内注射大肠埃希菌建立前列腺炎症模型,注入细菌1～14 d为急性炎症期,4～ 12周为慢性炎症期,对照组注入等量PBS.建模28 d后经静脉单纯注入BMSCs、单纯行MEUS及行MEUS+注入BMSCs,活体成像观察BMSCs向前列腺归巢情况.观察移植BMSCs 2周后大鼠前列腺组织病理学变化并作炎症评分,RT-PCR检测炎症因子IL-1β、TNF-α mRNA,ELISA检测IL-1β、TNF-α蛋白含量,统计分析各组表达差异.结果 前列腺组织病理学提示CBP组、CBP+ BMSCs组、CBP+ MEUS组前列腺呈典型炎症病理变化,腺管上皮增生,层次增加,炎细胞浸润,纤维组织增生,而CBP+ MEUS+ BMSCs组大鼠前列腺炎症明显减轻.RT-PCR检测结果提示CBP组炎症因子IL-1β(0.282±0.067)、TNF-α(0.631 ±0.116),CBP+BMSCs组IL-1[β(0.893 ±0.117)、TNF-α(0.876 ±0.137),CBP+ MEUS组IL-1β(0.683 ±0.097)、TNF-α(0.814±0.127)高于正常对照组IL-1β(0.282±0.067)、TNF-α(0.256±0.059)和CBP+MEUS+BMSCs组IL-1β(0.322±0.086)、TNF-α(0.313 ±0.079) (P ＜0.05);ELISA检测结果显示CBP组炎症因子IL-1β[(315.14±86.08) pg/mL]、TNF-α[(86.41 ±24.32) pg/mL],CBP+BMSCs组IL-1 β[(278.87±72.04) pg/mL]、TNF-α[(67.77±16.98) pg/mL],CBP+ MEUS组IL-1 β[(321.24 ±93.12) pg/mL]、TNF-α[(79.14±19.17) pg/mL]含量均显著高于正常对照组[IL-1β(43.36±11.35) pg/mL、TNF-α(13.26±3.97) pg/mL]和CBP+MEUS+BMSCs组[IL-1 β (65.34±17.23) pg/mL、TNF-α(19.94±5.37) pg/mL],差异有统计学意义(P＜0.01),而CBP+ MEUS+ BMSCs组与正常对照组比较差异无统计学意义(P＞0.05).结论 超声复合微泡促进BMSCs向前列腺归巢,能够减轻前列腺炎症反应,可能为前列腺炎的治疗带来新策略.     

润口液药理作用及刺激性的研究

润口液是一种用于解除生理性、病理性口干的制剂。实验研究结果表明，润口液具有促进唾液分泌，保持口腔湿润的功能，并且无毒、无刺激性。     

儿童先天性心脏病并发感染性心内膜炎的外科治疗

目的 探讨儿童先天性心脏病合并感染性心内膜炎的治疗经验。方法 本组患儿均使用多种抗生素,感染性心内膜炎（ＩＥ）未能控制,体温下降。于感染活动期手术治疗,摘除心内赘生物并纠正心内畸形。结果 ４例患儿施行手术治疗无手术死亡,术后效果满意,术后无复发。结论 儿童先天性心脏病合并感染性心内膜炎经内科治疗效果欠佳时,应尽早手术治疗。早期诊断及时手术是控制感染降低死亡率的主要措施。     

人参皂苷Rg1对阿霉素诱导系膜细胞凋亡的影响

目的:探讨人参皂苷Rg1对阿霉素毒性诱导的人肾小球系膜细胞(HRMC)凋亡的影响及其可能的调控机制。方法:实验分为5组:正常对照组、模型组及低、中、高剂量人参皂苷Rg1与阿霉素共孵育干预组。HRMC培养24 h后采用CCK-8法检测细胞的存活率,流式细胞术Annexin V/PI双染法检测细胞凋亡率,Real-Time PCR检测HRMC细胞凋亡相关基因Bcl-2、caspase-3相对表达水平。结果:阿霉素可显著诱导HRMC凋亡;中、低剂量的人参皂苷Rg1可减少HRMC的凋亡率,并可上调Bcl-2基因水平下调caspase-3水平;高剂量的人参皂苷Rg1促进HRMC的凋亡,在降低Bcl-2表达的同时caspase-3呈高表达。结论:阿霉素具有直接的肾毒性作用。通过增加Bcl-2和抑制caspase-3的表达,低、中剂量的人参皂苷Rg1可有效抑制阿霉素诱导的系膜细胞凋亡,且中剂量效果显著。