全文获取类型
收费全文 | 159篇 |
免费 | 13篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 8篇 |
妇产科学 | 7篇 |
基础医学 | 23篇 |
口腔科学 | 15篇 |
临床医学 | 12篇 |
内科学 | 26篇 |
皮肤病学 | 4篇 |
神经病学 | 10篇 |
外科学 | 11篇 |
综合类 | 2篇 |
预防医学 | 21篇 |
眼科学 | 8篇 |
药学 | 16篇 |
中国医学 | 4篇 |
肿瘤学 | 4篇 |
出版年
2023年 | 2篇 |
2022年 | 8篇 |
2021年 | 6篇 |
2020年 | 11篇 |
2019年 | 11篇 |
2018年 | 14篇 |
2017年 | 8篇 |
2016年 | 11篇 |
2015年 | 3篇 |
2014年 | 4篇 |
2013年 | 12篇 |
2012年 | 14篇 |
2011年 | 12篇 |
2010年 | 4篇 |
2009年 | 5篇 |
2008年 | 10篇 |
2007年 | 7篇 |
2006年 | 4篇 |
2005年 | 8篇 |
2004年 | 10篇 |
2003年 | 3篇 |
2002年 | 4篇 |
2001年 | 1篇 |
排序方式: 共有172条查询结果,搜索用时 31 毫秒
11.
Moustafa Gibory MSc Jennifer L. Dembinski PhD Elmira Flem MD PhD Ildri Haltbakk MSc Susanne G. Dudman MD PhD 《Journal of medical virology》2020,92(12):3151-3156
Acute gastroenteritis (AGE) is a common illness in both adults and children worldwide and is caused by several microorganisms including viruses, bacteria, and parasites. Rotavirus (RV), which is the main cause of AGE, can occur as a mixed infection with other viruses. The aim of this study is to assess the molecular epidemiology of viral enteric viruses and assess RV coinfections with other enteric viruses and their influence on disease severity before and after RV vaccine introduction in children under 5 years of age. A total of 600 samples collected from children hospitalized for AGE in five large hospitals in Norway, and were analyzed for viral gastroenteritis agents by enzyme immunoassay and quantitative real-time polymerase chain reaction (qRT-PCR). Positive results confirmed either by Sanger sequencing or genotyped by multiplex semi-nested RT-PCR. In total, 243 of the 300 (81%) samples, collected from the prevaccine cohort, were positive for at least one of the four viruses tested in this study. RV was most frequently identified in 82.6% of the samples. In the postvaccine cohort, 114 of the 300 (38%) samples were positive for at least one of the viruses tested. RV found in 36.5% of the samples. Coinfections found less frequently in the postvaccine cohort. Among circulating enteric viruses in Norway, RV is the most important cause of viral gastrointestinal infection. As expected, there were fewer RV positive and fewer coinfections after RV vaccine implementation. The results provide valuable data that can aid in further evaluation of the vaccine impact. 相似文献
12.
Background
Estimates of baseline incidence of childhood intussusception could help safety monitoring after the introduction of rotavirus vaccines. We studied the incidence of intussusception in Uzbekistan, a GAVI-fund eligible state in Central Asia. 相似文献13.
Isakbaeva ET Khetsuriani N Beard RS Peck A Erdman D Monroe SS Tong S Ksiazek TG Lowther S Pandya-Smith I Anderson LJ Lingappa J Widdowson MA;SARS Investigation Group 《Emerging infectious diseases》2004,10(2):225-231
To better assess the risk for transmission of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV), we obtained serial specimens and clinical and exposure data from seven confirmed U.S. SARS patients and their 10 household contacts. SARS-CoV was detected in a day-14 sputum specimen from one case-patient and in five stool specimens from two case-patients. In one case-patient, SARS-CoV persisted in stool for at least 26 days after symptom onset. The highest amounts of virus were in the day-14 sputum sample and a day-14 stool sample. Residual respiratory symptoms were still present in recovered SARS case-patients 2 months after illness onset. Possible transmission of SARS-CoV occurred in one household contact, but this person had also traveled to a SARS-affected area. The data suggest that SARS-CoV is not always transmitted efficiently. Routine collection and testing of stool and sputum specimens of probable SARS case-patients may help the early detection of SARS-CoV infection. 相似文献
14.
Talay Kudaiberdiev Gulzad Imanalieva Gulbarchin Usupbaeva Rahat Kalieva Elmira Tukusheva 《Central European Journal of Medicine》2010,5(3):369-371
Hypothyroidism in pregnancy is associated with serious maternal and fetal risk. Rarely, it is manifested by life-threatening
cardiac complications, such as gross pericardial effusion and tamponade. We present a case of successfully treated gross pericardial
effusion and tamponade in a 22-week pregnant woman with hypothyroidism. The patient was treated by pericardial drainage with
further treatment of hypothyroidism with levothyroxine. During the follow-up pregnancy was uncomplicated without recurrence
of pericardial effusion and successful delivery of full-term baby. We conclude that careful monitoring of thyroid functional
tests and proper management should be performed in pregnant women with hypothyroidism to prevent cardiac complications of
the disease, like pericardial effusion and tamponade. 相似文献
15.
Karalyan ZA Djaghatspanyan NG Gasparyan MH Hakobyan LA Abroyan LO Ter-Pogossyan ZR Zakaryan HS Karalova EM 《Microbial pathogenesis》2011,50(5):243-251
The resistance to picornaviral infection cells of susceptible lines has similar changes in the phenotype. They have decreased number of nucleoli and increased percentage of euploidy. Also the percentage of euploid cells those were resistant to the picornaviral infection increased in all highly transformed cultures. In resistant cells of all cultures has been found reduction of DNA. RNA amount also decreased both in nucleus and in cytoplasm. All these data correlated with the increased euploidy of the resistant population. The resistant cells had a less transformed phenotype, and decreased proliferative activity. Decreased nucleolar status became apparent by reduction of absolute and relative nucleolar indices. Consequently the reduction of viral titer (viral titters reduction) in resistant cells could be the direct result of diminished activity of the RNA synthesis machinery. It is important to note that the cells lose resistance while another type of virus, even from the same family, infects the culture once. 相似文献
16.
17.
Navimipour EJ Oskoee SS Oskoee PA Bahari M Rikhtegaran S Ghojazadeh M 《Lasers in medical science》2012,27(2):305-311
Success in sandwich technique procedures can be achieved through an acceptable bond between the materials. The aim of this
study was to compare the effect of 35% phosphoric acid and Er,Cr:YSGG laser on shear bond strength of conventional glass-ionomer
cement (GIC) and resin-modified glass-ionomer cement (RMGIC) to composite resin in sandwich technique. Sixty-six specimens
were prepared from each type of glass-ionomer cements and divided into three treatment groups as follows: without pretreatment,
acid etching by 35% phosphoric acid for 15 s, and 1-W Er,Cr:YSGG laser treatment for 15 s with a 600-μm-diameter tip aligned
perpendicular to the target area at a distance of 1 mm from the surface. Energy density of laser irradiation was 17.7 J/cm2. Two specimens in each group were prepared for evaluation under a scanning electron microscope (SEM) after surface treatment
and the remainder underwent bonding procedure with a bonding agent and composite resin. Then the shear bond strength was measured
at a crosshead speed of 0.5 mm/min. Two-factor analysis of variance and post-hoc Tukey test showed that the cement type, surface
treatment method, and the interaction of these two factors significantly affect the shear bond strength between glass-ionomer
cements and composite resin (p < 0.05). Surface treatment with phosphoric acid or Er,Cr:YSGG laser increased the shear bond strength of GIC to composite
resin; however, in RMGIC only laser etching resulted in significantly higher bond strength. These findings were supported
by SEM results. The fracture mode was evaluated under a stereomicroscope at ×20. 相似文献
18.
Kujundzic E Matalkah F Howard CJ Hernandez M Miller SL 《Journal of occupational and environmental hygiene》2006,3(10):536-546
In-room air cleaners (ACs) and upper-room air ultraviolet germicidal irradiation (UVGI) are engineering control technologies that can help reduce the concentrations of airborne bacteria and fungal spores in the indoor environment. This study investigated six different types of ACs and quantified their ability to remove and/or inactivate airborne bacteria and fungal spores. Four of the air cleaners incorporated UV lamp(s) into their flow path. In addition, the efficacy of combining ACs with upper-room air UVGI was investigated. With the ventilation system providing zero or six air changes per hour, the air cleaners were tested separately or with the upper-room air UVGI system in operation in an 87-m3 test room. Active bacteria cells and fungal spores were aerosolized into the room such that their numbers and physiologic state were comparable both with and without air cleaning and upper-room air UVGI. In addition, the disinfection performance of a UV-C lamp internal to one of the ACs was evaluated by estimating the percentage of airborne bacteria cells and fungal spores captured on the air filter medium surface that were inactivated with UV exposure. Average airborne microbial clean air delivery rates (CADRm) varied between 26-981 m3 hr-1 depending on the AC, and between 1480-2370 m3 hr-1, when using air cleaners in combination with upper-room air UVGI. Culturing, direct microscopy, and optical particle counting revealed similar CADRm. The ACs performed similarly when challenged with three different microorganisms. Testing two of the ACs showed that no additional air cleaning was provided with the operation of an internal UV-C lamp; the internal UV-C lamps, however, inactivated 75% of fungal spores and 97% of bacteria cells captured in the air filter medium within 60 min. 相似文献
19.
Kamyab AR Shahrokhi F Shamsian E Hayat Nosaied M Dibajnia P Hashemi M Mahdian R 《Clinical biochemistry》2012,45(3):267-271
ObjectiveTo compare the gene dosage results achieved by a novel multiplex quantitative assay with cytogenetic and quantitative fluorescent polymerase chain reaction (QF-PCR) analysis for prenatal screening of trisomy 21 syndrome on corresponding fetal samples.Design and methodsFetal samples (n = 134) were collected from pregnant women considered high risk for having trisomy 21 affected fetus. Cytogenetic analysis and QF-PCR were performed. Then, the relative gene dosage of DSCAM and DYRK1A2 genes was determined on corresponding samples using comparative delta cycle of threshold (ΔCT) method.ResultsThe mean gene dosage ratio was 1.55 ± 0.11 (95% CI:1.51–1.58) in trisomy 21 cases and 1.01 ± 0.12 (95% CI:0.98–1.03) in normal samples (p value < 0.001). The results were in agreement to the results of cytogenetic and QF-PCR analysis with the overall specificity of 0.96 (95% CI:0.91–0.98) and the sensitivity of 0.80 (95% CI:0.49–0.94).ConclusionsThis gene dosage assay is appropriate for the screening of high risk pregnant women and is readily amenable to automation. 相似文献
20.
Latipov R Kuatbaeva A Kristiansen O Aubakirova S Akhanaeva U Kristiansen IS Flem E 《Vaccine》2011,29(24):4175-4180