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11.
Intramolecular Alkylations of Aromatic Compounds, XVII1): — Synthesis of trans-3,10b-Dimethyl-1,2,3,4,4a,5,6,10b-octahydrobenzo(f)isoquinoline From the cyanhydrine 5b , easily obtained from 4 and trimethylsilyl cyanide, the lactone 6 is prepared, hydrogenation of which gives a mixture of the lactam 7a and the lactone 8 . Compound 7a is reduced to the secondary base 9 , which by treatment with acid is dehydrated to 10 and rearranges to give the isomer 11 , too. Attempted cyclization of the aminoalcohol 12 fails to give 9 or 10 . Rather, the furan 13 is isolated as the final product. N-methylation 10 → 14 and subsequent hydrogenation furnish the 4:1 trans/cis-mixture 2a, b , from which the title compound is separated by column chromatography. From 11 the stereomers 18a, b can be prepared analogously. 相似文献
12.
Hoschek Stefan Hoschek-Risslegger Ursula Fiegl Michael Zabernigg August Pall Georg Auberger Thomas Gunsilius Eberhard Schmid Thomas Jamnig Herbert Hilbe Wolfgang 《Wiener Medizinische Wochenschrift》2007,157(21):562-568
Wiener Medizinische Wochenschrift - Aggressives Tumorwachstum, frühe Metastasierung und hohe Assoziation mit intensivem Nikotinkonsum sind die Charakteristika des kleinzelligen... 相似文献
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A. Pansky F. Peng M. Eberhard L. Baselgia W. Siegrist J. B. Baumann A. N. Eberle C. Beglinger & P. Hildebrand 《European journal of clinical investigation》1996,27(1):69-76
Bombesin was originally isolated from amphibian skin, wherease its mammalian counterpart, gastrin-releasing peptide (GRP), was first identified in the nervous system of the gastrointestinal tract. Whether GRP is present in the human skin is not known. Bombesin-like peptides are also known to modulate growth. We therefore investigated whether human melanoma cell lines express functional GRP-preferring bombesin receptors and whether they alter growth or other specific cellular functions of these tumour cells. GRP receptor mRNA was found in HBL, D-10, Me-28 and A375-6 cell lines, but only A375-6 cells express a large number of high-affinity binding sites for [125 I]-[Tyr4 ] bombesin ( K d 0.31 ± 0.04 nmol L−1 , 3880 ± 429 binding sites per cell). Bombesin dose-dependently increased cytosolic calcium, but did not alter interleukin (IL) 1β-induced reduction of cell viability or IL-6 secretion, both A375-6-specific cell functions. Growth of A375-6 cells was not altered by bombesin or the specific GRP receptor antagonist BIM26226 as measured by [3 H]-thymidine incorporation or methylene blue assay, whereas insulin alone or in combination with other potential growth factors dose-dependently stimulated growth of these cells. The newly characterized GRP-preferring bombesin receptors on highly malignant human melanoma cells could initiate studies of growth effects on solid tumours or in vivo scanning using radiolabelled tracers. 相似文献
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Alexander Mülsch Eberhard Bassenge Rudi Busse 《Naunyn-Schmiedeberg's archives of pharmacology》1989,340(6):767-770
Summary Release of nitric oxide (NO) from endothelial cells critically depends on a sustained increase in intracellular free calcium maintained by a transmembrane calcium influx into the cells. Therefore, we studied whether the free cytosolic calcium concentration directly affects the activity of the NO-forming enzyme(s) present in the cytosol from freshly harvested porcine aortic endothelial cells. NO was quantified by activation of a purified soluble guanylate cyclase coincubated with the cytosol. In the presence of 1 mM L-arginine, 0.1 mM NADPH and 0.1 mM EGTA, endothelial cytosol (0.2 mg of cytosolic protein per ml) stimulated the activity of guanylate cyclase 5.0 + 0.5-fold (from 31 + 9 to 153 + 15 nmol cyclic GMP formed per min per mg guanylate cyclase). Calcium chloride increased this stimulation further in a concentration-dependent fashion by up to 136 + 15% (with 2 M free calcium; EC50 0.3 M). The calcium-dependent and -independent activation of guanylate cyclase was enhanced by superoxide dismutase (0.3 M) and was inhibited by the stereospecifically acting inhibitor of L-arginine-dependent NO formation NG-nitro-L-arginine (1 mM) and by LY 83583 (1 M), a generator of superoxide anions. Our findings suggest a calcium-dependent and -independent synthesis of NO from L-arginine by native porcine aortic endothelial cells.
Send of fprint requests to A. Mülsch, at the above address 相似文献
17.
Epidemiology of Cyclospora cayetanensis and other intestinal parasites in a community in Haiti
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Lopez AS Bendik JM Alliance JY Roberts JM da Silva AJ Moura IN Arrowood MJ Eberhard ML Herwaldt BL 《Journal of clinical microbiology》2003,41(5):2047-2054
We conducted an exploratory investigation in a community in Haiti to determine the prevalence of Cyclospora cayetanensis infection and to identify potential risk factors for C. cayetanensis infection. In 2001, two cross-sectional stool surveys and a nested case-control study were conducted. In 2002, a follow-up cross-sectional stool survey was conducted among children < or =10 years of age. Stool specimens from study participants and water samples from their wells were examined for Cyclospora and other intestinal parasites. In stools, the prevalence of infection with Cyclospora in persons of all ages decreased from 12% (20 of 167 persons) in February 2001 to 1.1% (4 of 352 persons) in April 2001, a 90.8% decrease. For children < or =10 years of age, the prevalence rates were 22.5% (16 of 71 children) in February 2001, 3.0% (4 of 135 children) in April 2001, and 2.5% (2 of 81 children) in January 2002. Use of the water from the artesian well in the northern region of the community versus the one in the south was the only risk factor associated with Cyclospora infection in multivariate analyses (odds ratio, 18.5; 95% confidence interval, 2.4 to 143.1). The water sample from one of the nine wells or water sources tested (one sample per source) in January 2001, shortly before the investigation began, was positive for Cyclospora by UV fluorescence microscopy and PCR. None of the water samples from the 46 wells or water sources tested during the investigation (one sample per source per testing period, including the artesian wells) were positive for Cyclospora. Further studies are needed to assess the role of water as a possible risk factor for Cyclospora infection in Haiti and other developing countries. 相似文献
18.
Heinz Gögelein Eberhard Schlatter Rainer Greger 《Pflügers Archiv : European journal of physiology》1987,409(1-2):122-125
Besides the larger Cl– channel with a single channel conductance of about 45 pS, a small channel was observed in the luminal membrane of the dogfish rectal gland [9]. In cell excised (inside out) patches with NaCl solution on both sides, the latter channel had a single channel conductance of 11±1 pS (n=21), and its current-voltage relationship was linear in the voltage range+90 to –90 mV. The open state probability increased moderately with negative clamp potentials. Ionic replacement studies revealed a high selectivity of Cl– over gluconate, sulfate, and iodide, whereas bromide was permeable to some extent. Also the channel is impermeable for Na+. The Cl– channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoate did not affect this small conductance Cl– channel. It can be concluded that the luminal membrane of stimulated rectal gland cells possesses two types of Cl– channels, which differ markedly in their characteristics.Supported by Deutsche Forschungsgemeinschaft Gr 480/8 and by NSF and NIH grants to the MDIBL 相似文献
19.
20.
The first and second internal transcribed spacers (ITS1, ITS2) as well as the intervening 5.8S coding region of the rRNA
gene were characterized in eight Babesia canis isolates of differing geographic origin, vector specificity, and pathogenicity to dogs. The genotypes determined by sequencing
segregated into three clearly separated groups close to or near the species level and correspond to the previously proposed
subspecies B. canis canis, B. canis vogeli, and B. canis rossi. The three genotypes can be distinguished by Sau96I digestion of the polymerase chain reaction (PCR)-amplified rDNA target.
Received: 12 December 1997 / Accepted: 5 January 1998 相似文献