氧应激在大鼠乙醇及四氯化碳致慢性肝损伤中的作用

目的:复制大鼠乙醇及四氯化碳(CCl4)致慢性肝损伤模型,观察氧应激反应在其发生中的作用.方法:50只,♂ Wistar大鼠平均分为A-E 5组,实验持续8 wk.A组生理盐水灌胃;B组乙醇灌胃(560 mL/L);C组在B组基础上同时PGE1腹腔内注射;D组乙醇(560 mE/L,)灌胃4 wk,然后CCl4腹腔注射4 wk.E组在D组基础上同时PGE1腹腔内注射.实验4、8 wk结束时分别检测全血GSH-Px,血清ALT、AST、ALP、TBA、TG、CHO、MDA.8 wk结束时处死动物,留取肝脏标本,行光镜及电镜检查.结果:5组大鼠的原始体质量在统计学上无显著性差异,实验结束时,与A组相比,B组和D组的最终体质量和增加体质量明显降低(249.00 g±18.83 g,258.50 g±20.28 g vs319.00 g±29.61 g;65.00 g±15.28 g,76.50 g±15.82 g vs 134.00 g±21.58 g,均P<0.01),肝质量占体质量百分比明显增高(3.267%±0.3165%,4.735%±0.7567%vs2.736%±0.1988%.均P<0.01),相应PGE1干预后(C组和E组),大鼠的生长缓慢、肝脏肿大情况得到明显改善(均P<0.01);B组和D组血清中的ALT、AST、ALP、TBA、TG和CHO含量发生不同程度的升高(P<0.01或0.05),PGE1干预后增高水平明显下降;乙醇和CCl4能引起外周血GSH-Px降低和MDA升高(均P<0.01),PGE1干预后能够得到明显改善,而PGE1的干预则可明显减轻乙醇和CCl4引起的肝脏病理学改变.结论:氧应激反应可能在大鼠乙醇及CCl4致慢性肝损伤过程中发挥重要作用,抑制氧应激反应可能是缓解肝损伤的重要措施.     

Apolipoprotein A5 gene polymorphisms are associated with non-alcoholic fatty liver disease

Background: Several studies have reported that apolipoprotein A5(APOA5) is involved in the development of non-alcoholic fatty liver disease(NAFLD). However, no research has been performed regarding the association between APOA5 polymorphisms and the risk of NAFLD. This study aimed to explore the association between APOA5 gene polymorphisms and NAFLD in a Chinese Han population.Methods: Genotypes of the SNPs(rs10750097, rs1263173, rs17120035, rs3135507 and rs662799) of APOA5 in 232 NAFLD patients and 188 healthy controls were determined using polymerase chain reaction(PCR)analysis. Clinical characteristics were measured using biochemical methods.Results: The five single nucleotide polymorphisms(SNPs)(rs10750097, rs1263173, rs17120035, rs3135507 and rs662799) of APOA5 showed no significant association with NAFLD(P 0.05). The rs10750097 with G allele showed a higher serum level of alkaline phosphatase(ALP) compared with C allele in overall series and NAFLD patients(P 0.05). The rs1263173(A/A) carriers showed a higher level of glucose compared to the non-carriers in overall series(P 0.05). The rs17120035(T/T) carriers showed a lower plasma TG level in overall series and NAFLD patients(P 0.05), and the rs662799(G/G) carriers showed higher levels of plasma triglyceride(TG), ALP, and lower level of high-density lipoprotein(HDL) compared to non-carriers in NAFLD patients(P 0.05). No significant difference were observed on the clinic parameters of APOA5 rs3135507(T/T) carriers in both group of overall series and NAFLD patients(P 0.05).Conclusions: The five SNPs(rs10750097, rs1263173, rs17120035, rs3135507 and rs662799) of APOA5 gene are not associated with the risk of NAFLD in the Chinese Han population. The genotypes of rs10750097(G/G), rs1263173(A/A), rs17120035(T/T), and rs662799(G/G) performed a significant effect on clinic characteristics in overall series and NAFLD patients, indicating that these polymorphisms may be associated with NAFLD.     

Molecular Characterization of the First Alternavirus Identified in Fusarium oxysporum

A novel mycovirus named Fusarium oxysporum alternavirus 1(FoAV1) was identified as infecting Fusarium oxysporum strain BH19, which was isolated from a fusarium wilt diseased stem of Lilium brownii. The genome of FoAV1 contains four double-stranded RNA (dsRNA) segments (dsRNA1, dsRNA 2, dsRNA 3 and dsRNA 4, with lengths of 3.3, 2.6, 2.3 and 1.8 kbp, respectively). Additionally, dsRNA1 encodes RNA-dependent RNA polymerase (RdRp), and dsRNA2- dsRNA3- and dsRNA4-encoded hypothetical proteins (ORF2, ORF3 and ORF4), respectively. A homology BLAST search, along with multiple alignments based on RdRp, ORF2 and ORF3 sequences, identified FoAV1 as a novel member of the proposed family “Alternaviridae”. Evolutionary relation analyses indicated that FoAV1 may be related to alternaviruses, thus dividing the family “Alternaviridae” members into four clades. In addition, we determined that dsRNA4 was dispensable for replication and may be a satellite-like RNA of FoAV1—and could perhaps play a role in the evolution of alternaviruses. Our results provided evidence for potential genera establishment within the proposed family “Alternaviridae”. Additionally, FoAV1 exhibited biological control of Fusarium wilt. Our results also laid the foundations for the further study of mycoviruses within the family “Alternaviridae”, and provide a potential agent for the biocontrol of diseases caused by F. oxysporum.     

Effects of traditional Chinese medicine exercise therapy on cancer-related fatigue,anxiety and sleep quality in cancer patients: A protocol for systematic review and network meta-analysis

Background:Cancer-related fatigue (CRF) is one of the most common adverse events of anticancer therapies, with an incidence of up to 90%, which seriously affects the quality of life in cancer patients. Complementary and alternative therapies for CRF include acupuncture, Chinese herbal medicine, Tai Chi, Qigong, and massage therapy. Several studies have shown that traditional Chinese medicine (TCM) exercise therapies, such as Tai Chi, Ba Duan Jin, the classics of tendon changing, Six Healing Sounds, and Wu Qin Xi, can improve CRF, anxiety symptoms, and sleep quality in cancer patients. However, their specific efficacy and which TCM exercise therapy is the best remain controversial. In this study, we assess and compare the effects of different TCM exercise therapies on CRF, anxiety, and sleep quality in cancer patients by network meta-analysis (NMA).Methods:Randomized controlled trials reporting TCM exercise therapies for CRF, anxiety and sleep quality in cancer patients published before October 2021 will be searched in the PubMed, Web of Science, Scopus, Cochrane Library, Embase, China Scientific Journal Database, China National Knowledge Infrastructure, Chinese Biomedical Literature Database, and Wanfang Data. Two researchers will be independently responsible for literature screening, data extraction and assessment of their quality. Standard pair-wise and NMA will be performed to compare the efficacy of different TCM exercise therapies on CRF, anxiety and sleep quality in cancer patients using Stata 14.0 software.Results:The results of this meta-analysis will be submitted to a peer-reviewed journal for publication.Conclusions:This NMA will contribute to identify the optimal TCM exercise therapy for CRF and provide evidence-based bias for clinical decision-making.Ethics and dissemination:Ethical approval was not required for this study. The systematic review will be published in a peer-reviewed journal, presented at conferences, and shared on social media platforms.OSF REGISTRATION NUMBER:DOI 10.17605/OSF.IO/MJ8DA.     

Efficacy of sequential N-butylphthalide therapy on psychiatric and behavioral functions in acute ischemic stroke

Background:Stroke can cause physical and mental problems. This study examined how the sequential therapy of N-butylphthalide (NBP) could effectively improve physical movement, life activities, and psychological disorders in stroke patients.Methods:This double-blind, randomized controlled trial included middle-aged or elderly patients with acute ischemic stroke that had commenced within 48 hours before enrolment in the study. The experimental group was administered 100 mL NBP injections twice a day in the first 14 days, and a sequential 200 mg NBP soft capsule 3 times a day for the next 76 days. The control group was administered 100 mL NBP placebo injections twice a day in the first 14 days and 200 mg sequential NBP placebo soft capsule 3 times a day for the next 76 days. Primary outcomes were the National Institutes of Health Stroke Scale, the Barthel Index of activities of daily living, and Modified Rankin Scale which were evaluated at day 0, day 14, and month 1 or at day 14, month 3, and month 6. Secondary outcomes included the Hamilton Anxiety Scale and the Hamilton Depression Scale, all were evaluated on day 0, month 3, and month 6. Moreover, the adverse reaction of NBP or other serious adverse events were evaluated at each time.Results:Our therapy significantly increased the Barthel Index of activities of daily living scores, decreased the National Institutes of Health Stroke Scale and Modified Rankin Scale scores, and the incidence of the Hamilton Anxiety Scale and the Hamilton Depression Scale of ischemic stroke patients (P < .05).Conclusion:Our results indicated that 90 days’ sequential therapy with NBP as an additional therapy in the treatment of ischemic stroke can better improve patients’ psychological and behavioral functions without significant side effects.     

沙美特罗-丙酸氟替卡松治疗慢性阻塞性肺疾病的疗效分析

目的 分析沙美特罗-丙酸氟替卡松治疗慢性阻塞性肺疾病(COPD)的临床疗效及对STAT通路蛋白的影响;方法 纳入我院2017年4月至2020年11月期间诊治的COPD患者76例,随机分为沙美特罗组25例、丙酸氟替卡松组27例和沙美特罗联合丙酸氟替卡松联合组24例.沙美特罗组给予沙美特罗治疗,丙酸氟替卡松组给予丙酸氟替卡...     

载脂蛋白E基因敲除模型小鼠转化生长因子β1表达与尼古丁的刺激

背景：研究表明尼古丁可以诱导动脉粥样硬化,但其具体作用机制尚不明确。目的：探讨不同剂量尼古丁刺激下,载脂蛋白E基因敲除小鼠主动脉粥样斑块形成与外周血转化生长因子β1水平的关系。方法：载脂蛋白E基因敲除小鼠腹膜下注射2种不同剂量的尼古丁[2mg/（kg·d）及0.5mg/（kg·d）]12周,对照组注射等量生理盐水。用ELISA法检测外周血转化生长因子β1水平,苏木精-伊红染色观察动脉粥样斑块的病理变化。结果与结论：尼古丁干预12周后,低剂量尼古丁组的外周血转化生长因子β1水平低于对照组（P〈0.05）,高剂量尼古丁组的外周血转化生长因子β1水平低于对照组及低剂量尼古丁组（P〈0.05）。高剂量尼古丁组的血管狭窄程度最严重,低剂量尼古丁组次之,对照组最低,各组间比较差异均具有显著性意义（P〈0.05）。低剂量尼古丁组及高剂量尼古丁组的斑块个数多于对照组（P〈0.05）,高剂量尼古丁组的斑块个数与低剂量尼古丁组比较差异无显著性意义（P〉0.05）;血管狭窄率与转化生长因子β1水平呈负相关（r=-0.920,P=0.000）。结果提示尼古丁刺激增加载脂蛋白E基因敲除小鼠动脉粥样硬化斑块数量,这种作用可能与尼古丁抑制转化生长因子β1水平相关。     

脊髓损伤后截瘫病人建立家庭护理病房的可行性研究

[目的]研究脊髓损伤后截瘫病人建立家庭护理病房的可行性。[方法]将53例本市脊髓损伤后截瘫病人按时间顺序分为对照组和研究组,两组出院时按常规进行出院指导,对照组定期电话随访;研究组上门进行康复护理及相关技能指导,建立家庭护理病房。[结果]两组并发症发生情况及日常生活自理能力比较差异有统计学意义。[结论]家庭护理病房对脊髓损伤后截瘫病人康复有较好的促进作用,能有效改善肢体运动功能,增强其活动能力,降低并发症发生率,提高病人生活质量。     

护生与临床带教老师人际沟通影响因素研究

人际沟通中的障碍会阻止或歪曲信息的传递,障碍可能来源于环境,也可能来源于信息发送者或接受者[1].师生沟通是指老师与护生在教学环境中发生的信息传递、交流和理解过程,是教学活动的主要表现形式和手段,是完成教学任务的重要保证[2].因此,寻找护生与带教老师在人际沟通中存在的问题,进行归纳、总结,并采取措施避免这些问题的出现,对改进带教方式、提高临床带教质量有重大的意义.为此,我们对2009年—2011年在我院临床实习的100名护生和100名带教老师进行调查.现报告如下.     

磁共振乳腺动态增强曲线在乳腺疾病诊断中的价值

目的:探讨磁共振乳腺动态增强曲线在磁共振检查乳腺疾病诊断中的应用价值。材料与方法:对25例乳腺疾病的患者进行磁共振平扫及动态增强扫描检查,扫描采用GE公司HDe 1.5T磁共振机配备8通道乳腺线圈进行检查,动态增强采用团注12时相扫描法检查。结果:25例乳腺病变均经过病理证实,其中恶性病变6例,动态增强曲线表现为Ⅲ型4例,Ⅱ型2例,良性病变19例,Ⅰ型曲线17例,Ⅱ型曲线2例。结论:乳腺动态增强曲线在磁共振乳腺疾病的诊断中提供了可靠的依据,结合动态增强后的病变征象,对提高乳腺疾病诊断的正确性起到了重要的价值。