全文获取类型
收费全文 | 2032篇 |
免费 | 135篇 |
国内免费 | 45篇 |
专业分类
耳鼻咽喉 | 16篇 |
儿科学 | 95篇 |
妇产科学 | 26篇 |
基础医学 | 141篇 |
口腔科学 | 42篇 |
临床医学 | 284篇 |
内科学 | 326篇 |
皮肤病学 | 44篇 |
神经病学 | 58篇 |
特种医学 | 288篇 |
外科学 | 292篇 |
综合类 | 205篇 |
预防医学 | 139篇 |
眼科学 | 16篇 |
药学 | 119篇 |
2篇 | |
中国医学 | 32篇 |
肿瘤学 | 87篇 |
出版年
2022年 | 30篇 |
2021年 | 32篇 |
2020年 | 30篇 |
2018年 | 21篇 |
2017年 | 22篇 |
2016年 | 18篇 |
2015年 | 33篇 |
2014年 | 48篇 |
2013年 | 74篇 |
2012年 | 77篇 |
2011年 | 85篇 |
2010年 | 91篇 |
2009年 | 104篇 |
2008年 | 69篇 |
2007年 | 78篇 |
2006年 | 81篇 |
2005年 | 52篇 |
2004年 | 48篇 |
2003年 | 49篇 |
2002年 | 46篇 |
2001年 | 36篇 |
2000年 | 27篇 |
1999年 | 28篇 |
1998年 | 82篇 |
1997年 | 74篇 |
1996年 | 72篇 |
1995年 | 67篇 |
1994年 | 41篇 |
1993年 | 50篇 |
1991年 | 19篇 |
1990年 | 14篇 |
1989年 | 30篇 |
1988年 | 42篇 |
1987年 | 39篇 |
1986年 | 29篇 |
1985年 | 33篇 |
1984年 | 19篇 |
1983年 | 24篇 |
1982年 | 16篇 |
1980年 | 16篇 |
1977年 | 15篇 |
1976年 | 14篇 |
1963年 | 17篇 |
1960年 | 14篇 |
1959年 | 22篇 |
1958年 | 29篇 |
1957年 | 19篇 |
1956年 | 30篇 |
1955年 | 42篇 |
1954年 | 35篇 |
排序方式: 共有2212条查询结果,搜索用时 15 毫秒
951.
952.
953.
Structural and mutational analysis of a conserved gene (DGSI) from the minimal DiGeorge syndrome critical region 总被引:2,自引:1,他引:2
Gong W; Emanuel BS; Galili N; Kim DH; Roe B; Driscoll DA; Budarf ML 《Human molecular genetics》1997,6(2):267-276
The majority of patients with DiGeorge syndrome (DGS), velocardiofacial
syndrome (VCFS), conotruncal anomaly face syndrome (CTAFS) and some
individuals with familial or sporadic conotruncal cardiac defects have
hemizygous deletions of chromosome 22. Most patients with these disorders
share a common large deletion, spanning > 1.5 Mb within 22q11.21-q11.23.
Recently, the smallest region of deletion overlap has been narrowed to a
250 kb area, the minimal DGS critical region (MDGCR), which includes the
locus D22S75 (N25). We have isolated and characterized a novel, highly
conserved gene, DGSI, within the MDGCR. DGSI has 10 exons and nine introns
encompassing 1702 bp of cDNA sequence and 11 kb of genomic DNA. The encoded
protein has 476 amino acids with a predicted mol. wt of 52.6 kDa. The
intron-exon boundaries have been analyzed and conform to the consensus
GT/AG motif. The corresponding murine Dgsi has been isolated and localized
to proximal mouse chromosome 16. The mouse gene contains the same number of
exons and introns, and the predicted protein has 479 amino acids with 93.2%
identity to that of the human DGSI gene. By database searching, both genes
have significant homology to a Caenorhabditis elegans hypothetical protein,
F42H10.7. Further, mutation analysis has been performed in 16 patients, who
have no detectable 22q11.2 deletion and some of the characteristic clinical
features of DGS/VCFS. We have detected eight sequence variants in DGSI.
These occurred in the 5'- untranslated region, the coding region and the
intronic regions adjacent to the intron-exon boundaries of the gene. Seven
of the eight variants were also present in normal controls or unaffected
family members, suggesting they may not be of etiologic significance.
相似文献
954.
955.
956.
Kelly CL; Rhead WJ; Kutschke WK; Brix AE; Hamm DA; Pinkert CA; Lindsey JR; Wood PA 《Human molecular genetics》1997,6(9):1451-1455
We report the therapeutic effects of liver-specific expression of a
short-chain acyl-CoA dehydrogenase (SCAD) transgene in the SCAD- deficient
mouse model. Transgenic mice were produced with a rat albumin
promoter/enhancer driving a mouse SCAD minigene (ALB-SCAD) on both the SCAD
normal genetic background and a SCAD-deficient background. In three
transgenic lines produced on the SCAD-deficient background, recombinant
SCAD activity and antigen in liver mitochondria were found up to 7-fold of
normal control values. All three lines showed a markedly reduced organic
aciduria and fatty liver, which are sensitive indicators of the metabolic
abnormality seen in this disease found in children. We found no detrimental
effects of high liver SCAD expression in transgenic mice on either
background. These studies provide important basic and practical therapeutic
information for the potential gene therapy of nuclear-encoded mitochondrial
enzyme deficiencies, as well as insights into the mechanisms of the
disease.
相似文献
957.
Dyment DA; Sadovnick AD; Ebers GC; Sadnovich AD$corrected to Sadovnick AD 《Human molecular genetics》1997,6(10):1693-1698
Multiple Sclerosis (MS) is a common chronic central nervous system disease
in young adults. Relative familial risk appears to be determined largely by
genes while population risk is strongly influenced by environmental
factors. This is supported by genetic epidemiological studies which also
suggest an oligogenic inheritance of susceptibility. The HLA DRB1*1501,
DQA1*0102, DQB1 0602 haplotype is associated with the disease but HLA
contributes only modestly to overall susceptibility. The results of three
genomic searches are concordant with the genetic epidemiology and imply a
number of genes with interacting effects will be found. Importantly, no
single region has been identified with a major influence on familial risk.
相似文献
958.
丙氧鸟苷对转TK基因的人外周血单个核细胞的毒性作用 总被引:1,自引:0,他引:1
目的 探讨减少异体干细胞移植中移植抗宿主病(GVHD),同时最大限度提高移植抗白血病(GVL)效应的有效方法。方法 采用逆转录病毒介导的基因转移方法将I型单纯疱疹病毒胸苷激酶(HSV-TK)基因、绿色荧光蛋白(GFP)基因及抗新霉素(NeoR)基因插入人外周血单个核细胞(PBMC),MTT法测定丙氧鸟苷(GCV)对转化细胞抑制率。结果 转化细胞表达GFP,且主要为T淋巴细胞,占11.4%,而且被转化的T淋巴细胞中,CD4阳性细胞转化率较高,占7.6%,CD8,CD19,CD33阳性细胞转化率分别为2.9%,2.1%和4.7%,PCR鉴定表明,转染的人外周血单个核细胞DNA中整合中有NeoR基因,MTT法测定丙氧鸟苷(GCV)对转化细胞与未转化细胞抑制率,显示转化细胞生长明显受抑。结论 异体干细胞移植后,如产生严重GVHD应用GCV选择性清除HSV-TK基因转导的T淋巴细胞,使控制GVHD已成可能。 相似文献
959.
A gradual loss of cells occurs within the humantrabecular meshwork (TM) during normal aging andappears to be increased in patients with primary openangle glaucoma (POAG) [1] .The exactmechanism bywhich cells are lost in either condition is not known,however phagocytosis has been suggested[2 ] .It hasbeen found that,when compared with the non- POAGpatients,the level of transforming growth factor- β2(TGF- β2 ) which may be involved in the pathogenesisof POAG increased in the aqueous humo… 相似文献
960.
This article has no abstract. To view the article, select the "View Print Version (PDF)" link above. 相似文献