原位末端标记检测实验性变态反应性神经炎胸腺细胞的凋亡

目的为了探讨地塞米松对实验性变态反应性神经炎（ＥＡＮ）小鼠胸腺细胞的影响。方法应用原位末端标记法和电镜检测ＥＡＮ小鼠胸腺细胞的凋亡。结果以上两种方法均发现地塞米松处理组胸腺细胞凋亡率明显高于自然病程组和正常对照组。结论地塞米松可明显增强ＥＡＮ小鼠胸腺细胞凋亡。     

Lysosomal dysfunction results in lamina-specific meganeurite formation but not apoptosis in frontal cortex

An inhibitor of cathepsins B and L was used to test if lysosomal dysfunction in cultured slices of rat frontal cortex induces pathological features that develop in the human cortex during aging and Alzheimer's disease (AD). Incubation for 6 days with N-CBZ-L-phenylalanyl-L-alanine-diazomethylketone (ZPAD) resulted in a massive proliferation of endosomes-lysosomes in all cortical layers. Slices additionally exposed to a washout of 4 days had numerous meganeurites, blister-like structures in the region of the axon hillock, in layer III but not in other cortical laminae. Meganeurites are a characteristic feature of the human frontal cortex after age 50 and are largely restricted to layer III. Tests for apoptosis were carried out at two intervals following meganeurite formation. TUNEL-labeled neurons were confined to layers II/III on the surface of the slices but there was no evidence for a ZPAD effect. In all, 6 days of lysosomal dysfunction reproduces characteristic effects of normal aging in neocortex without generating some key features of AD.     

c-fos基因在乐果中毒后肌无力大鼠肌组织中的表达

采用ＲＴ－ＰＣＲ、ＷｅｓｔｅｒｎＢｌｏｔ技术,检测了有机磷农药乐果对大鼠骨骼肌细胞中即刻早期反应基因ｃ－ｆｏｓ表达的影响。结果表明,在乐果作用下,骨骼肌细胞ｃ－ｆｏｓ基因表达在ｍＲＮＡ以及蛋白质水平均明显增高。然而骨骼肌细胞ｃ－ｆｏｓ的表达是否与毒物刺激有关,其在农药中毒机制上的生物学意义有待深入研究。     

胃癌增殖细胞核抗原表达的研究

目的 探讨增殖细胞核抗原（ＰＣＮＡ）与胃癌生物学行为的关系。方法 应用流式细胞术,对６８例胃癌及癌旁组织细胞中ＰＣＮＡ的表达进行了检测,结果 ＰＣＮＡ在胃癌中的表达明显高于正常胃粘膜及癌旁组织;ＰＣＮＡ指数与胃癌分化化程度及肿瘤浸润深度有关（Ｐ〈０．０５）,结论 检测ＰＣＮＡ的表达有助于发现早期癌变及有判断胃癌的生物学选择性。     

血糖监测仪测定微血管全血糖的临床研究

目的：研究血糖仪测定微血管全血糖与生化法测定静脉血清糖之间的差别以及相关性,验证血糖仪的准确性及稳定性。方法：用ＯＮＥＴＯＵＣＨⅡ血糖仪测定微血管全血糖（ＣＢＧ）３３７ 例（全部为２ 型糖尿病患者）,同时用葡萄糖氧化酶法测定静脉血清糖（ＶＳＧ）。结果：ＶＳＧ 高于ＣＢＧ１０．８％ ,ＶＳＧ 与ＣＢＧ 之间存在显著相关性,ｒ 为０．９５,Ｐ＜ ０．００１。结论：轻便血糖监测仪测ＣＢＧ快速,简便,对操作者技术要求不高,适用于糖尿病人血糖自我监测和糖尿病筛选、普查     

几种生长因子在人表皮细胞无血清培养中的作用

目的为生长因子在烧伤创伤面的应用提供理论依据。方法采用无血清培养系统,以3H－TdR掺入法测定表皮生长因子（EGF）、牛垂体提取物（BPE）和乙醇胺（Etha）对人表皮细胞DNA合成的影响。结果EGF和BPE在无血清培养基中对培养表皮细胞DNA合成有明显的促进作用（P＜0．01）;Etha对培养表皮细胞DNA合成也有促进作用（P＜0．05）;EGF和Etha在本培养体系中具有协同作用（P＜0．05）。结论生长因子在烧伤创面的治疗中具有应用价值。     

试论提高乡镇医院效益

通常乡镇医院的经济效益是由病人的经济支付能力,医院提供卫生服务的可及性和医生的作用三方面因素决定的。对此从理论和实践方面进行了探讨,得出”减轻病人负担,上门为群众提供多功能医疗服务,优势的医疗服务是提高乡镇医院效益的有效途径。     

Stereoselective synthesis of (±)-epibatidine analog: (±)-2β-(2-chloro-5-pyridinyl)-8-azabicyclo[3.2.1]octane

Stereoselective synthesis of (+/-)-epibatidine analog 2, which contains the 8-azabicyclo[3.2.1]octane ring system, was achieved by using palladium-catalyzed Heck-type coupling reaction from 3.     

Glutathione conjugates of 2- or 6-substituted 5,8-dimethoxy-1,4-naphthoquinone derivatives: Formation and structure

Thirty-four glutathione conjugates of 5,8-dimethoxy-1,4-naphthoquinones (DMNQ) were synthesized and their structure was determined. The yield of GSH conjugate was dependent on size of alkyl group; the longer the size of alkyl group was, the lower was the yield. It was also found that the length of alkyl side chain influenced the chemical shift of quinonoid protons; the quinonoid protons of 2-glutathionyl DMNQ derivatives with R=H to propyl, 6.51-6.59 ppm vs. other ones with R=butyl to heptyl, 6.64-6.68 ppm. This was explained to be due to a folding effect of longer alkyl group. Glutathione (GSH) reacted with DMNQ derivative first to form a 1,4-adduct (2- or 3-glutathionyl-1,4-dihydroxy-5,8-dimethoxynaphthalenes) and then, the adduct was autooxidized to 2- or 3-glutathionyl-DMNQ derivatives. Moreover, GSH reduced DMNQ derivatives to their hydrogenated products. It was suggested that such an organic reaction might play an important role for a study of metabolism or toxicity of DMNQ derivatives in the living cells.