肿瘤免疫治疗时代的多重免疫组织化学/免疫荧光技术

常规免疫组织化学(immunohistochemistry,IHC)是组织病理学中广泛应用的诊断技术.但该技术存在诸多局限性,包括观察者间的变异性高,每张组织切片仅能标记一个标志物.本文详细描述了各种多重检测技术,这些技术克服了上述局限性,可在单张切片上同时检测多个标志物,可对细胞组成、细胞功能和细胞间相互作用进行整合...     

Biological variation and reference change values of serum Mac‐2–binding protein glycosylation isomer (M2BPGi)

BackgroundLimited data are available with regard to biological variations of the Mac‐2–binding protein glycosylation isomer (M2BPGi), a liver fibrosis biomarker.MethodsLong‐term biological variation of M2BPGi was investigated using longitudinally measured M2BPGi test results from healthy Korean adult subjects. One‐way analysis of variance (ANOVA) tests were used to calculate the reference change value (RCV) of M2BPGi based on biological variation estimates. Furthermore, asymmetric RCV was calculated according to a recent publication of the European Federation of Clinical Chemistry and Laboratory Medicine Working Group on Biological Variation and Task Group for the Biological Variation Database (EFLM TG‐BVD).ResultsA total of 363 test results from 174 Korean subjects undergoing general health checkups were requested from 13 local clinics and hospitals during a 38‐month period. The within‐subjects biological variation (CV_I), between‐subject biological variation (CV_G), analytical variation (CV_A), RCV, and individuality index (II) values for serum M2BPGi were 23.3%, 30.0%, 4.3%, 65.6%, and 0.78, respectively. Asymmetric RCV calculated using formulae by a recent EFLM TG‐BVD publication ranged from −41.9 to 72.0%. Desirable analytical performance specifications for M2BPGi derived from biological variation were as follows: imprecision 11.6%, bias 9.6%, and total allowable error 28.7%.ConclusionsRCV based on biological estimates may be helpful for evaluating and interpreting serial M2BPGi measurements by physicians and in clinical laboratories.     

LncRNA SLC16A1‐AS1 contributes to the progression of hepatocellular carcinoma cells by modulating miR‐411/MITD1 axis

BackgroundHepatocellular carcinoma (HCC) is a common malignancy with high morbidity. The current study aimed to explore the molecular mechanism of lncRNA SLC16A1‐AS1 in the tumorigenesis of HCC.Material and methodsThe expression of SLC16A1‐AS1 and miR‐411 was examined in clinical HCC tissues. HCC cell lines Hep3B and Huh‐7 were employed and transfected with si‐SLC16A1‐AS1. The correlation between SLC16A1‐AS1 and miR‐411 was verified by luciferase reporter assay. Cell viability was detected by CCK‐8 assay. Cell migration and invasion capacity were examined by transwell assay. The protein level of MITD1 was analyzed by western blotting.ResultsThe expression of SLC16A1‐AS1 markedly increased in HCC tissues and cell lines. Subsequent studies identified SLC16A1‐AS1 as a downstream target of miR‐411. In addition, SLC16A1‐AS1 knockdown and miR‐411 overexpression significantly stagnated the progression of HCC cells. SLC16A1‐AS1 knockdown also downregulated MITD1 levels.ConclusionOur findings showed that SLC16A1‐AS1 was overexpressed in HCC cells and tissues. SLC16A1‐AS1 promoted the malignant characteristics of HCC cells and acted as an oncogene. Its regulatory effect may be associated with miR‐411/MITD1 axis. Therefore, SLC16A1‐AS1 has the potential to be used as a biomarker or therapeutic target for the treatment of HCC.     

动脉顺应性评价降压疗效的临床意义

目的 探讨动脉顺应性评价降压疗效的临床意义。方法 受试者均为男性，分为正常对照组72例；高血压控制和未控制组各35例，使用美国产HDI－DO－2020动脉测试仪同步记录血压和动脉顺应性（大动脉弹性C1，小动脉弹性C2）。结果 高血压未控制组：收缩压（SBP），舒张压(DBP），平均动脉压（MAP）和脉压（PP）均明显高于正常对照组和高血压控制组（P均＜0．01）；而对照组和高血压控制组之间无统计学差异（P＞0．05）。高血压未控制组的C1和C2顺应性明显降低，与对照组和高血压控制组比较有统计学差异（P均＜0．01）；同样后两组之间无学差异（P＞0．05）。结论 动脉面应性可作为评价降低疗效的敏感指标。     

高三尖杉酯碱对Ph+树突状细胞成熟与功能的影响

目的：观察不同浓度的高三尖杉酯碱(HHT)对慢性粒细胞白血病(CML)Ph^ 树突状细胞(DC)成熟与功能的影响。方法：采用Ficoll密度离心法分离制备单个核细胞(MNC)，在体外诱导扩增DC，在培养体系中添加不同浓度的HHT，通过细胞化学染色、流式细胞仪检测细胞表面分子表达水平、^3H—TdR掺入法检测细胞功能等方法，对所得细胞进行鉴定。结果：CML DC存在表型及功能障碍。在培养体系中添加HHT，能获得较多成熟CML DC，提高其细胞表面共刺激分子CD80以及黏附分子LFA-2的表达水平，并可改善其刺激淋巴细胞增殖的能力。结论：适合浓度的HHT可影响Ph^ DC的分化和成熟，并可改变DC细胞表面免疫分子的表达，增强其功能，     

α-三噻吩对白纹伊蚊幼虫的毒杀作用

目的　研究α三噻吩对白纹伊蚊幼虫的杀灭效果、影响因素及其对白纹伊蚊幼虫生长发育的影响。　方法　在实验室特定近紫外光 (UVA)下,计数不同药物浓度、不同黑暗处理时间的α三噻吩作用后蚊幼虫的死亡数、化蛹数及蛹的羽化数;在户外自然光下,观测不同药物浓度、不同施药时间的α三噻吩作用后蚊幼虫的死亡数。　结果　实验室特定UVA下,α 三噻吩对白纹伊蚊幼虫的半数致死量为2.37μg/L;药物与幼虫在黑暗中作用3h后再施以UVA照射能发挥其最大毒杀作用;α三噻吩能够显著抑制白纹伊蚊幼虫的发育和蛹的羽化。户外自然光下,高浓度α 三噻吩在强烈阳光辐射下可以快速杀灭白纹伊蚊幼虫,上午5时施药后24h的毒杀作用显著优于上午10时和下午1时。　结论α三噻吩是一种高效、实用、并能够抑制蚊幼虫生长发育的杀虫剂。     

Significance of inducible ventricular flutter/fibrillation in risk stratification in patients with coronary artery disease

BACKGROUND: Although inducible ventricular fibrillation (VF) has been used as an indication for prophylactic implantation of cardioverter-defibrillators (ICDs) in patients with coronary artery disease (CAD), the significance of inducible VF remains controversial. METHODS: Among 364 CAD patients who underwent electrophysiologic (EP) study for risk stratification, 23 patients, 12 without any history of VF or cardiac arrest (group A) and 11 with previously documented VF or cardiac arrest (group B), exhibited inducible ventricular flutter (VFL) or VF and subsequently underwent ICD implantation. Additionally, 11 CAD patients without previous VF or cardiac arrest, who had no inducible ventricular tachyarrhythmias but received an ICD, were included for comparison (group C). RESULTS: During 2 years of follow-up, 1 (8%), 5 (45%), and 1 (9%) patients had appropriate ICD shocks in groups A, B, and C, respectively. The survival free from appropriate ICD shocks was significantly lower in group B compared to groups A and C (p<0.05). There were no significant differences in age, sex, ejection fraction (EF), or induction protocol between groups A and B or between groups A and C. CONCLUSIONS: In CAD patients with inducible VFL/VF, patients without any history of VF or cardiac arrest had significantly lower incidence of appropriate ICD shocks when compared to those with such clinical events. Conversely, in CAD patients without any history of VF or cardiac arrest, incidence of appropriate ICD shocks was similar regardless of inducible VFL/VF. Inducible VFL/VF is therefore not useful as an indication for prophylactic ICD implantation in this patient population.     

70岁以上老年急性髓性白血病的治疗及预后分析

目的探讨70岁以上老年急性髓性白血病临床及生物学特征、影响预后的因素及个体化治疗方案。方法对我院1994~2005年收治58例老年AML患者(≥70岁)回顾性分析了其临床及生物学特征、影响预后的因素及治疗方案的比较。结果亚标准剂量组(A组)33例,减量组(B组)9例,支持治疗组(C组)8例。A组与B组缓解率差异无统计学意义(P>0.05),分别为39.4%,22.2%。生存期明显异常(P=0.01),分别为167天和68天。C组生存期176天,与亚标准化疗组差异无统计学意义(P>0.05),但化疗引起副反应明显减少,平均住院时间缩短(P<0.05)。预后因素分析表明:生存状态,继发性,PBWBC>10×109/L,骨髓原始细胞比率>50%,染色体核型,免疫表型是预后的显著影响因素。结论70岁以上AML患者有特殊的临床和生物学特征,治疗应个体化,其预后与多种因素有关。     

First-trimester fetal limb biometry in Chinese population

OBJECTIVES: To establish reference ranges for fetal limb biometry obtained by transabdominal ultrasound examination at 12-14 weeks of gestation in Chinese women. METHODS: A total of 1489 normal singleton fetuses between 12(+0) and 14(+6) weeks of gestation were examined transabdominally by two experienced observers. The gestation was ascertained by measurement of fetal crown-rump length (CRL) or biparietal diameter (BPD) and head circumference (HC). The lengths of the humerus and femur were prospectively worked out. RESULTS: Gestation-specific reference ranges for limb measurements were calculated. The femur length (FL) (mean +/- SD) was 9.15 +/- 1.44 mm at 12-12(+6) weeks, 11.45 +/- 1.35 mm at 13-13(+6) weeks, and 13.64 +/- 1.60 mm at 14-14(+6) weeks; the corresponding humerus length (HL) (mean +/- SD) was 9.63 +/- 1.44 mm, 12.01 +/- 1.41 mm, 14.29 +/- 1.48 mm, respectively. CONCLUSIONS: There was a general increase in fetal limb biometry with gestation. Fetal limb length can be an early marker of early growth restriction as well as fetal skeletal dysplasia among women in the local population.     

长三角一体化背景下智慧互联网医院建设与实践

目的/意义 针对国家长三角区域医疗一体化战略部署及长三角示范区协同发展政策方针,积极整合优质医疗资源保障资源供给、快速提升区域医疗服务水平与质量,设计长三角示范区互联网医院建设架构。方法/过程 以互联网医疗服务平台、远程医疗协同平台以及数据互联互通平台的设计为主要着手点,对现有医疗信息化资源进行合理规划和集成。结果/结论 依据方案建设的长三角(上海)智慧互联网医院运营效果良好。