内镜腔内手术的实验研究

目的探讨内镜腔内手术的可行性. 方法 6只家猪经皮建立胃内通道,腹腔镜引导下胃内模拟缝合止血操作实验. 结果 6只家猪均很好地耐受了手术.术后开腹检查所有胃壁缝合处无渗漏及其它脏器损伤.建立胃内通道时间、胃内缝合时间及总手术时间分别为(60.5±12.2)min、(26.5±5.78)min和(92.1±15.0)min. 结论内镜腔内手术在技术上是可行的.     

细胞移植的人嗅鞘细胞培养方法的研究

目的探讨适用于细胞移植的人嗅鞘细胞（OECs）分离与培养的方法。方法无菌条件下取自愿捐献孕5个月流产胎儿的完整嗅球进行嗅鞘细胞提取分离、纯化，并对嗅鞘细胞进行免疫组化染色及纯度检测，观察其形态学特点，检测其免疫组化染色的阳性率。结果本方法分离、培养的嗅鞘细胞大多呈三角形、梭形，有些突起较长，生长活跃，增殖迅速，P75、胶质纤维酸性蛋白（GFAP）免疫组化染色呈阳性，嗅鞘细胞阳性率为95．3％。结论该方法简便易行且不影响细胞功能，适用于细胞移植的研究。     

4种黏结剂对牙本质黏结强度的比较研究

目的:比较两种第六代黏结剂-XenoⅢ、AdaperPrompt与两种第五代黏结剂-Singlebond、Singlebond2两两之间黏结强度的差异,为临床选择黏结剂提供参考依据。方法:暴露新鲜拔除的牛牙根牙本质,按照上述4种黏结剂各自的操作要求进行黏结,测试标本的微拉伸强度(μTBS)和剪切强度。结果:XenoⅢ、AdaperPrompt、Singlebond和Singlebond2的微拉伸强度(MPa)依次为:20.87±4.88,24.81±5.24,30.41±3.48,31.61±6.95;剪切强度依次为:14.85±4.26,17.23±2.57,19.18±5.65,20.92±3.73。结论:第五代黏结剂较第六代黏结剂具有更高的黏结强度,推荐使用。     

Antisense Smad2 inhibits high glucose-stimulated production of extracellular matrix in cultured rat glomerular mesangial cells

Transforminggrowthfactor-β1(TGF-β1)is amultifunctionalpolypeptidethatregulatesanum-berofcellularprocesses,includingcellprolifera-tion,differentiation,apoptosis,migration,matrix synthesis,andtheimmuneresponse[1,2].Inchron-icrenaldiseases,TGF-β1isakeymediatorofex-tracellularmatrix(ECM)accumulation[3].Oneof thetargetrenalcellsforTGF-β1isglomerular mesangialcellsthatarecapableofproducingcom-ponentsofECM,suchascollagens,lamininand fibronectin[4,5].Recentstudiesindicatedthatinhi-bitionofT…     

Effects of the AMPA receptor antagonist NBQX on the development and expression of behavioral sensitization to cocaine and amphetamine

 We examined the effect of 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (NBQX), an antagonist of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) subtype of glutamate receptor, on the development and expression of behavioral sensitization to amphetamine and cocaine in rats. A single injection of NBQX (12.5 mg/kg) administered 30 min prior to cocaine during the induction phase (days 1–5) prevented the development of cocaine sensitization, assessed by responsiveness to cocaine challenge on day 8. This NBQX regimen did not affect development of amphetamine sensitization. Two pretreatment injections of NBQX, one 20 min before and one 70 min after amphetamine on each day of the induction phase (days 1–6), did not affect sensitization of stereotypy but prevented sensitization of post-stereotypy ambulatory hyperactivity (both assessed by responsiveness to amphetamine challenge on day 8). The effect of NBQX on ambulatory sensitization was dose-dependent (attenuation with 12.5 mg/kg, complete prevention with 25 mg/kg). In contrast to its effects on development, NBQX (25 mg/kg) did not prevent expression of sensitization to cocaine or amphetamine. NBQX itself exerted no significant effects on locomotor activity in either drug-naive rats or rats that had received either NBQX or amphetamine repeatedly. These findings support a requirement for AMPA receptor stimulation in the development of locomotor sensitization to cocaine and amphetamine, but suggest a different mechanism for sensitization of amphetamine stereotypy. Received: 14 January 1997 / Final version: 24 June 1997     

双头加压螺纹钉贴股骨距内固定治疗股骨颈骨折

在Ｘ光电视下采用双头加压螺纹钉贴股骨距内固定治疗新鲜股骨颈骨折４２例，随访平均２．５年，其中１６例随访３年以上，骨折愈合率９５．２％，骨折不愈合并股骨头缺血性坏死率４．７６％。认为加压螺纹钉贴股骨距内固定可使钉的受力由较大剪应力变为较大轴向力承受，减少骨折处的剪应力，并加压于骨折端能促进骨折愈合。具有操作简单、进钉准确、创伤小、固定牢靠、并发症少等优点。     

Stimulation of locus coeruleus increases arterial pressure in rabbits

目的：研究电刺激和化学刺激兔蓝斑（ＬＣ）对动脉血压（ＡＰ）和肾交感神经传出活动（ＲＳＡ）的影响．方法：电刺激ＬＣ，ＬＣ微量注射ＬＧｌｕ、盐酸吗啡、ＧＡＢＡ、电解毁损ＬＣ，记录ＡＰ和ＲＳＡ．结果：电刺激ＬＣ和ＬＣ注射ＬＧｌｕ均引起ＡＰ升高（分别为１３５±０３ｖｓ１９５±０８ｋＰａ和１３８±０４ｖｓ１７５±０８ｋＰａ）和ＲＳＡ增加．ＬＣ注射吗啡、ＧＡＢＡ对ＡＰ和ＲＳＡ无明显影响．电解毁损ＬＣ后电刺激ＬＣ区、ＬＣ区注射ＬＧｌｕ对ＡＰ和ＲＳＡ无明显影响．结论：兔ＬＣ兴奋引起ＡＰ升高和ＲＳＡ增加，但ＬＣ不是ＡＰ和ＲＳＡ的紧张性中枢．     

外伤性急性颈椎间盘突出症(附19例报告)

本文旨在阐明MRI对急性颈椎间盘突出的诊断价值。19例均有明显的外伤史,X线片无骨折脱位者12例。其中C_(5～6),髓核突出7例,C_(5～6)、C_(1～5),突出5例。有小关节绞锁半脱位者7例,MRI均示损伤间隙有髓核突出。19例均为不全瘫。全部病例经前路椎间盘切除、植骨融合。平均随访15个月,植骨块平均于12周愈合。除1例骨块退出4～5mm外,余位置良好。脊髓功能均有明显恢复。结论:外伤是急性颈椎间盘突出的主要原因,而颈椎间盘突出又是SCIWORA综合征的主要原因。对小关节绞锁半脱位者应考虑同时有椎间盘突出的可能。手术治疗一般效果良好。     

海南省第四次全国结核病流行病学抽样调查分析

目的：评价世行贷款结核病项目实施效果，掌握疫情现状，为制度2001－2010年防治规划提供理论依据。方法：按照《第四次全国结核病流行病学抽样调查工作手册》对2个随机抽样点人群进行流调。结果：活动性肺结核13例，患病率和涂阳患病率434．8／10万，33．4／10万，较1990年流设疫情分别下降了43．4％和27．3％，BCG接种率34．9％，较1996年流调提高了一倍。结论：以控制传染源为目的：以DOTS为核心的结核病控制策略是成功的，可行的，政府的行政干预及保持必要的经费投入，对结核病控制可持续发展具有重要意义。农村儿童及新生儿BCG接种工作亟待改善。