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51.
Motohiro Akagi K. Inui Toshinori Nishigaki Takashi Muramatsu Chikara Kokubu L. Fu Hisao Fukushima Itaru Yanagihara Hiroko Tsukamoto Hiroki Kurahashi Shintaro Okada 《Journal of human genetics》1999,44(5):323-326
Fucosidosis is a rare autosomal recessive disorder resulting from a deficiency of α-L-fucosidase. Recently, various mutations
have been reported in this disease, but it is difficult to elucidate the phenotype from the genetic mutations. We report a
patient with chronic infantile type fucosidosis, with a compound heterozygote of a nonsense mutation (W148X, Trp at codon
148 to stop codon) and a large deletion, including all exons. This is the first report of a large deletion demonstrated in
fucosidosis. It is interesting that this patient has a relatively mild clinical course despite the absence of the mRNA. This
case also indicates the difficulty in determining the phenotype from the genotype in fucosidosis.
Received: February 19, 1999 / Accepted: April 16, 1999 相似文献
52.
Clinicopathological features of gastric carcinoma in younger and middle-aged patients: A comparative study 总被引:2,自引:0,他引:2
Chikara Kunisaki M.D. Ph.D. Hirotoshi Akiyama M.D. Ph.D. Masato Nomura M.D. Goro Matsuda M.D. Yuichi Otsuka M.D. Hidetaka Andrew Ono M.D. Ph.D. Ryo Takagawa M.D. Yutaka Nagahori M.D. Ph.D. Masazumi Takahashi M.D. Ph.D. Fumihiko Kito M.D. Ph.D. Hiroshi Shimada M.D. Ph.D. 《Journal of gastrointestinal surgery》2006,10(7):1023-1032
Gastric carcinoma is relatively rare in patients under the age of 40. This study was undertaken to clarify the clinicopathological
characteristics and surgical outcomes of gastric carcinoma in younger patients compared with those of middle-aged patients.
The surgical results from 131 younger patients (aged ⩽40 years) and 918 middle-aged patients (aged 55–65 years) were compared
retrospectively. Female gender, undifferentiated tumor type and lymphatic invasion were significantly more common in the younger
patients. Survival time did not differ between the two groups. The depth of tumor invasion was the only prognostic factor
in younger patients, whereas macroscopic appearance, tumor diameter, depth of invasion, lymph node metastasis, and venous
invasion were all significant prognostic factors in middle-aged patients. Peritoneal recurrence was significantly more common
in younger patients. A family history of gastric adenocarcinoma was observed in 25.9% of younger patients, but this did not
affect survival outcomes. As depth of invasion affects prognosis independently, and peritoneal metastasis is the predominant
pattern of recurrence, it is essential to establish an optimal prophylactic treatment for peritoneal metastasis to improve
surgical outcomes in younger patients with advanced gastric cancer. 相似文献
53.
Shinichi Hasegawa MD Takaki Yoshikawa MD PhD Yasushi Rino MD Takashi Oshima MD PhD Toru Aoyama MD Tsutomu Hayashi MD Tsutomu Sato MD Norio Yukawa MD Yoichi Kameda MD PhD Takeshi Sasaki MD PhD Hidetaka Ono MD PhD Kazuhito Tsuchida MD Haruhiko Cho MD Chikara Kunisaki MD PhD Munetaka Masuda MD PhD Akira Tsuburaya MD 《Annals of surgical oncology》2013,20(13):4252-4259
Objective
The purpose of this study was to clarify the priority of nodal dissection in Siewert types II and III adenocarcinoma of the esophagogastric junction (AEG).Methods
The priority of nodal dissection was evaluated based on the therapeutic value index calculated by multiplying of the frequency of metastasis to each station and the 5-year survival rate of patients with metastasis to that station.Results
A total of 176 patients (95 type II and 81 type III) were examined. Among the lymph nodes that had a metastatic incidence exceeding 10 %, the stations showing the first to fourth highest index were the paracardial and lesser curvature nodes (Nos. 1, 2, and 3) and the node at the root of the left gastric artery (No. 7) in the total cohort, as well as in each type. The next station was the lower thoracic paraesophageal lymph node (No. 110), followed by the nodes along the proximal splenic artery (No. 11p) in type II, whereas it was the nodes along the proximal splenic artery (No. 11p) followed by the para-aortic nodes (No. 16a2), the nodes at the celiac artery (No. 9), and the nodes around the splenic hilum (No. 10) in type III.Conclusions
These results suggest that the highest priority nodal stations to be dissected were the paracardial and lesser curvature nodes (Nos. 1, 2, and 3) and the nodes at the root of the left gastric artery (No. 7), regardless of the Siewert subtype, but the subsequent priority was different depending on the subtype. 相似文献54.
Fukuda N Nakayama M Jian T Satoh C Nakayama T Soma M Izumi Y Kanmatsuse K 《American journal of hypertension》2003,16(2):129-134
Insulin resistance is involved in the pathogenesis of type 2 diabetes, hypertension, and atherosclerosis. Angiotensin (Ang) converting enzyme inhibitors and Ang II type 1 receptor antagonists improve insulin resistance in patients with essential hypertension, which suggest that tissue Ang II is involved in insulin resistance in patients with hypertension. To investigate the participation of tissue Ang II in insulin resistance associated with hypertension, we evaluated the Ang II-generating system in leukocytes and its relation to insulin resistance in patients with essential hypertension. Eighteen patients with essential hypertension participated in this study. Ang II was separated from leukocytes by reversed-phase high-performance liquid chromatography and measured by radioimmunoassay. Insulin resistance was evaluated by determining the steady-state of plasma glucose (SSPG) concentration. The Ang I- and Ang II-generating activities were evaluated in human leukocytes. Human leukocytes have Ang I- and Ang II-generating activities. The Ang II-generating activity was significantly inhibited by pepstatin A. Leukocyte Ang II level does not correlate with BP or plasma Ang II level in patients with essential hypertension. Leukocyte Ang II level strongly correlates with SSPG concentration, and significantly correlates with body mass index and plasma insulin, and with leptin levels in patients with essential hypertension. Leukocyte Ang II may be directly associated with insulin resistance. 相似文献
55.
The biphasic effect of extracellular glucose concentration on carbachol‐induced fluid secretion from mouse submandibular glands 下载免费PDF全文
Momomi Terachi Chikara Hirono Michinori Kitagawa Makoto Sugita 《European journal of oral sciences》2018,126(3):197-205
Cholinergic agonists evoke elevations of the cytoplasmic free‐calcium concentration ([Ca2+]i) to stimulate fluid secretion in salivary glands. Salivary flow rates are significantly reduced in diabetic patients. However, it remains elusive how salivary secretion is impaired in diabetes. Here, we used an ex vivo submandibular gland perfusion technique to characterize the dependency of salivary flow rates on extracellular glucose concentration and activities of glucose transporters expressed in the glands. The cholinergic agonist carbachol (CCh) induced sustained fluid secretion, the rates of which were modulated by the extracellular glucose concentration in a biphasic manner. Both lowering the extracellular glucose concentration to less than 2.5 mM and elevating it to higher than 5 mM resulted in decreased CCh‐induced fluid secretion. The CCh‐induced salivary flow was suppressed by phlorizin, an inhibitor of the sodium–glucose cotransporter 1 (SGLT1) located basolaterally in submandibular acinar cells, which is altered at the protein expression level in diabetic animal models. Our data suggest that SGLT1‐mediated glucose uptake in acinar cells is required to maintain the fluid secretion by sustaining Cl? secretion in real‐time. High extracellular glucose levels may suppress the CCh‐induced secretion of salivary fluid by altering the activities of ion channels and transporters downstream of [Ca2+]i signals. 相似文献
56.
57.
Yoshitomo Kashiwagi Chikara Kikuchi Jun-ichi Anzai 《Journal of electroanalytical chemistry (Lausanne, Switzerland)》2002,518(1):51-55
Electrocatalytic dehalogenation of organohalides was studied using a nickel(II) tetraazamacrocyclic complex-modified graphite felt electrode. The nickel(II) tetraazamacrocyclic complex-modified graphite felt electrode was prepared by attaching nickel(II) (6-(2′-hydroxyethyl)-1,4,8,11-tetraazacyclotetradecane)perchlorate chemically to the carboxyl groups of a thin poly(acrylic acid) layer coated on the graphite felt. The modified electrode gave a reversible electron transfer for the nickel(II)/nickel(I) redox couple in cyclic voltammetry at ?0.95 V versus Ag/AgCl. A preparative electrocatalytic dehalogenation of organohalides was successfully achieved on the modified electrode with an adequate current efficiency (55.6–94.8%), conversion (34.2–100%) and turnover number of the Ni catalyst (667–3333). 相似文献
58.
59.
Kitamura C Takahashi M Kondoh Y Tashiro H Tashiro T 《Journal of neuroscience research》2007,85(11):2385-2399
Activity-dependent gene expression is one of the key mechanisms of synaptic plasticity that form the basis of higher order functions such as learning and memory. In the present study, we surveyed for activity-dependent genes by analyzing gene expression changes accompanying reversible inhibition of synaptic activity by tetrodotoxin (TTX) using two types of DNA microarrays; our focused oligo DNA microarray "Synaptoarray" and the commercially available high-density array. Cerebral cortical cells from E18 rat embryos were cultured for 14 days to ensure synaptogenesis, then treated with 1 muM TTX for 48 hr without detectable effect on cell viability. Synaptic density estimated by the amount of Synapsin I and Synaptotagmin I was decreased 21-24% by TTX treatment, but recovered to the control level 48 hr after TTX withdrawal. Comparison of gene expression profiles by competitive hybridization of fluorescently labeled cRNA from TTX-treated and control cells showed an overall downregulation of the genes on the Synaptoarray by TTX-treatment with different recovery rates after TTX withdrawal. With 16 representative genes, microarray data were validated by real-time PCR analysis. Genes most severely downregulated by TTX and upregulated above the control level at 5 hr after TTX withdrawal were munc13-1 (involved in docking and priming of synaptic vesicles) and Shank2 (involved in the postsynaptic scaffold). In addition, comprehensive screening at 5 hr after TTX withdrawal using high density arrays resulted in additional identification of Rgs2, a regulator of trimeric G-protein signaling, as an activity-dependent gene. These three genes are thus likely to be key factors in the regulation of synaptic plasticity. (c) 2007 Wiley-Liss, Inc. 相似文献