Treatment of 30 cases of vitiligo by cupping method plus external application of Chinese herbs

在皮损区拔罐,然后外涂中药治疗白癜风患者30例,对照组予西药治疗,3个疗程后,治疗组总有效率96.7%,对照组总有效率76.7%.治疗组疗效好于对照组(P＜0.05).     

再谈肺癌中血管生成与其预后的关系

目的:研究肺癌中血管内皮生长因子(vascularendothelialgrowthfactorVEGF)、激酶插入结构域受体(ki鄄naseinsertdomainingreceptorKDR)表达水平和微血管密度(Micro-vasculardensityMVD)及其与肺癌预后的关系。方法:采用免疫组化方法(LSAB法),检测114例肺癌组织中的VEGF、KDR表达水平及MVD。结果:肺癌组织中VEGF、KDR高表达组患者5年生存率分别为18.18%和21.19%,显著低于低表达组61.19%和52.74%(P<0.01);MVD高密度组患者5年生存率为17.27%,显著低于低密度组52.74%(P<0.01)。VEGF、KDR表达水平及MVD与生存期均呈显著负相关(P<0.01),其相关强度依MVD、VEGF、KDR顺序逐渐降低。多因素分析表明,pTNM分期仍是预测肺癌预后最有价值的指标,但VEGF、KDR表达水平,MVD也是判断肺癌预后较有价值的指标。VEGF、KDR表达水平越高,MVD越大,其无瘤生存期和5年生存率越短。结论:肺癌中VEGF、KDR基因的表达异常及MVD的增大在肺癌的发生、发展、转移和预后中可能起重要作用,检测肺癌中VEGF、KDR基因表达水平及MVD有助于预测肺癌预后。     

Isolation and characterization of propagable cell lines (HUNC) from the androgen-sensitive Dunning R3327H rat prostatic adenocarcinoma

The Dunning H rat prostate tumor (R3327H) is a widely used experimental model of human prostatic adenocarcinoma (CaP). The Dunning H tumor has been characterized as androgen-sensitive, androgen-receptor (AR) positive, prostate-specific antigen and prostatic acid phosphatase (PAP) positive. To date, the tumor has been maintained by serial passage in vivo because of the lack of an in vitro cell line that retains the characteristics of the in vivo tumor. The objective of the present study was to establish a propagable cell line from R3327H adenocarcinoma that maintained androgen sensitivity and expression of AR, PSA and PAP. Tissue harvested from an in vivo R3327H tumor was dissociated with collagenase and placed into Richter's improved media (with supplements). A cytokeratin-positive epithelial cell line (HUNC- E) and a vimentin-positive stromal cell line (HUNC-S) were generated from the primary culture, subcultured continuously for >300 days, and passaged >50 times. Survival of the HUNC-E cell line in vitro depended on several media supplements, including nicotinamide, insulin, transferrin, selenium and epidermal growth factor (EGF). HUNC-E cells expressed AR and produced PSA and PAP throughout the culture period, as confirmed by immunocytochemistry and Western blot analyses. Addition of 14 nM testosterone (T) or dihydrotestosterone (DHT) to HUNC-E cells, stimulated DNA synthesis as well as anchorage-independent growth and PSA production, which demonstrated the androgen-sensitive nature of the cells in vitro. When HUNC-E and HUNC-S cells were combined in a 3:1 ratio and introduced subcutaneously into syngeneic male hosts, tumors formed in 2/3 animals with an average latency of 7 months. RT-PCR and immunocytochemical characterization of the HUNC cell lines revealed that the cells expressed several growth factors and their cognate receptors, including HGF, TGF-alpha and the TGF-betas, indicating the establishment of potential autocrine loops in the neoplastic cells. The HUNC-E and HUNC-S CaP cell lines, which retain the characteristics of the epithelial and stromal components of the in vivo R3327H tumor, will allow a more thorough and informative molecular and biological analysis of prostatic adenocarcinoma.      

Zinc replenishment reduces esophageal cell proliferation and N- nitrosomethylbenzylamine (NMBA)-induced esophageal tumor incidence in zinc-deficient rats

Previous work has shown that sustained increased and decreased cell proliferation, induced by dietary zinc deficiency and caloric restriction respectively, influence the course of N- nitrosomethylbenzylamine (NMBA)-induced esophageal carcinogenesis in rats. The present study considered whether the increased cell proliferation and esophageal tumor incidence induced by zinc deficiency are reversed upon zinc replenishment. Weanling rats were maintained initially on a deficient diet containing 4 p.p.m. zinc. After 5 weeks, carcinogen-treated animals were given six intragastric doses of NMBA (2 mg/kg twice weekly). Controls were untreated. After the second NMBA dose, the rats were divided into three dietary groups. One group was continued on the deficient diet, while the other two groups were switched to diets containing either 75 or 200 p.p.m. zinc, with half of the members in each group fed ad libitum and half pair-fed with deficient rats. NMBA-untreated controls were similarly replenished. At various time points, esophageal cell proliferation was assessed in five animals from each group by immunohistochemical detection of cells in S phase, with in vivo 5-bromo-2'deoxyuridine labeling. At 11 weeks after the first dose, esophageal tumor incidence was greatly reduced, from 100% in the deficient group to 26 and 14% respectively in the replenished groups fed ad libitum 75 and 200 p.p.m. zinc and to 14 and 11% respectively in the replenished groups pair-fed 75 and 200 p.p.m. zinc. In addition, the number of tumors per esophagus was reduced from 9.93 +/- 4.25 in deficient rats, to a range of 0.11 +/- 0.31-0.30 +/- 0.54 in replenished animals. Following zinc replenishment, esophageal cell proliferation, as measured by labeling index (LI), the number of labeled cells and the total number of cells, was markedly decreased in NMBA-untreated and -treated esophagi as compared with those in corresponding deficient esophagi. Thus, the esophageal cell proliferation induced by zinc deficiency is reversed by zinc replenishment and replenished animals have a markedly lower incidence of esophageal tumors.      

Role of cyclin E and p53 expression in progression of early gastric cancer

Background. To elucidate the role that cyclin E overexpression plays in the progression of early gastric cancer, we examined the expression of cyclin E and p53, as abnormal p53 expression is linked with cyclin E overexpression in exerting adverse affects on the cell cycle. Methods. Specimens from 108 early gastric cancers were stained by an immunohistochemical method, using anti-cyclin E and anti-p53 antibodies. Results. The positivity rate of cyclin E expression in early gastric cancer was 33% (36/108). Cyclin E-positive tumors invaded more deeply (P < 0.05), infiltrated lymphatic vessels more frequently (P < 0.01), showed a higher incidence of differentiated cancer (P < 0.01), and more often expressed p53 (P < 0.01) than cyclin E-negative tumors. Differentiated cancers showing coexpression of cyclin E and p53 were more likely to metastasize to the lymph nodes. Conclusions. Overexpression of cyclin E may promote the progression of early gastric cancer. Received for publication on Apr. 27, 1998; accepted on Nov. 17, 1998     

早期隔上霍奇金淋巴瘤斗篷野与累及野放疗联合化疗比较

目的:比较早期隔上霍奇金淋巴瘤(HD)斗篷野与累及野放疗联合化疗的疗效.方法:对35例早期HD患者,先予化疗4个疗程,然后随机分为常规斗篷野放疗(20例)或累及野放疗(15例),剂量为大野30Gy,局部肿块区加至40Gy,放疗后再化疗2个疗程.结果:中位随访58个月.3年总生存率为94.3%.无复发生存率为80.0%.两组3年总生存率及无复发生存率无明显差异.结论:早期隔上HD在化疗基础上,累及野与斗篷野放疗疗效相似,并发症较低.     

肿瘤转移相关基因与周围型肺癌CT征象的关系研究

摘要目的：探讨VEGF和nm23-H1基因表达水平与周围型肺癌的病理生理学特征和CT征象之间的关系。方法：回顾性分析确诊的47例周围型肺癌患者CT表现．同时采用免疫组化方法(LSAB法)检测47例肺癌组织中的VEGF和nm23-H1表达水平。结果：1)VEGF、nm23-H1表达水平与原发肿瘤大小、淋巴结转移状态，pTNM分期均有密切关系(P＜0．05)。2)VEGF与nm23-H1表达水平呈负相关(P＜0.05)。3)VEGF基因的高表达和nm23-H1基因的低表达均与分叶征、纵隔淋巴结转移有关(P＜0．05)，且VEGF的高表达也与空洞征有关：二者表达水平与毛刺征、空泡征、胸膜凹陷征和支气管征均无关(P＞0．05)。4)深分叶征、空泡征和支气管征与肿瘤大小有关(P＜0．05)，而与毛刺征、胸膜凹陷征和纵隔淋巴结转移均无关(P＞0.05)。结论：VEGF过度表达和nm23-H1低表达均与周围型肺癌的分叶征、空洞坏死征和纵隔淋巴结转移有密切关系。     

人白细胞介素1β(IL-1β)在诱导人黑色素瘤A375-S2细胞凋亡过程中的细胞周期阻滞作用和对线粒体Bcl-2家族蛋白的调节

目的研究人白细胞介素1β(interleukin-1β,IL-1β)体外诱导人黑色素瘤A375-S2细胞凋亡的机制。方法通过Hoechst33258荧光染色,观察A375-S2细胞在IL-1β作用下的形态学变化。使用琼脂糖凝胶电泳法检测IL-1β对细胞DNA降解的影响。应用流式细胞分析技术研究IL-1β对A375-S2细胞周期的影响。利用Westernβlot方法检测IL-1β对细胞线粒体内Bcl-2家族蛋白表达的调节作用。结果IL-1β(1nM)能够诱导A375-S2细胞凋亡,72h时细胞体变小,细胞核呈现固缩、断裂,并出现因凋亡引起的DNA梯状条带。IL-1β可将细胞周期阻滞在G0/G1期,并具有时间依赖性。IL-1β诱导细胞凋亡过程中,抗凋亡蛋白Bcl-2和Bcl-xL的表达减少,促凋亡蛋白Bax的表达增加。结论IL-1β通过将细胞周期阻滞在G0/G1期,上调线粒体内Bax/Bcl-2和Bax/Bcl-xL的表达比例诱导A375-S2细胞凋亡。     

芙蓉Cu200C宫内节育器系统评估

目的：系统评估芙蓉（FR）Cu200C IUD的安全性、有效性和可接受性。方法：通过电子和手工检索查阅1988年4月～2006年5月国内外发表的相关文献，按照循证医学的原则进行筛选和评估。结果：共检索到22篇相关文献，按标准纳入5篇。纳入文献的结果显示。置器后1年，FRCu200C IUD的累积妊娠率略高于母体乐（ML）Cu375 IUD，因症取出率也趋向高于MLCu375 IUD；FRCu200C IUD的经量增多发生率低于MLCu375 IUD；置器后1、2、5年FRCu200C IUD的累积续用率均趋向低于MLCu375 IUD。结论：FRCu200C IUD的临床效果，尤其是有效性，接近或略差于MLCu375 IUD。建议在推广使用FRCu200C IUD前，对其开展多中心随机对照临床研究，进一步证实其安全性、有效性和可接受性；建议由非FRCu200C IUD研制单位主持临床试验，观察年限应≥5年。