p38 MAPK在LPS诱导牙龈成纤维细胞表达uPA中作用的研究

目的：研究p38 MAPK在LPS诱导牙龈成纤维细胞表达uPA中的作用。方法：采用Western blotting观察LPS对牙龈成纤维细胞内p38 MAPK活性的影响；蛋白激酶活性实验SB203580地p38 MAPK活性的抑制作用；Northern blotting观察SB203580对LPS诱导uPA表达的影响。结果：LPS能够迅速地激活牙龈成纤维细胞内p38 MAPK的活性；SB203580能够有效地抑制牙龈成纤维细胞内的p38 MAPK的活性；经SB203580处理后，LPS对uPA的诱导作用受到显著的抑制。结论：LPS通过p38 MAPK信号转导途径诱导牙龈成纤维细胞表达uPA。     

减阻牵张正畸牙移动过程中牙周组织超微结构变化的研究

目的：研究减阻牵张正畸牙移动过程中牙周组织超微结构的变化，为此方法矫治正畸牙提供理论依据。方法：将6只杂种犬随机分为2周、3周、6周二组，拔除下颌两侧第二前磨牙，随机选择一侧为实验侧，实施减阻牵张措施加快牙齿移动速度，另一侧为空白对照侧，实验侧加力2周、固定保持1周、4周时，制备移动牙标本，进行透射电镜观察。结果：对照侧无明显成骨及破骨迹象；加力2周组张力侧成骨细胞、成纤维细胞的胞浆内高尔基体、内质网等细胞器非常丰富，分泌功能旺盛；压力侧破骨细胞增多，高尔基体发达，线粒体、溶酶体增多，成纤维细胞细胞器发达，间充质细胞、活跃的巨噬细胞增多；随着固定保持时间的延长，实验侧与对照侧超微结构的差异变小。结论：正畸牙压力侧减阻后，在强、高频率加力方式作片用下，牙周组织细胞功能旺盛、改建活跃，无不可逆的细胞变性坏死变化。     

肝X受体作为肿瘤潜在靶点的研究进展

胆固醇堆积是癌症细胞的普遍特征.肝X受体(LXRs)属于核受体超家族成员和配体依赖性转录因子,是维持细胞内胆固醇稳态的关键分子,在糖代谢和炎症、免疫反应调节中也发挥重要作用.近年来越来越多的证据表明,LXRs参与众多恶性肿瘤的发生与进展病理过程,成为癌症治疗的新靶点.本文就LXRs在各种临床前肿瘤模型中的作用和机制研究作一综述,旨在为LXRs在肿瘤方面的基础及临床转化研究提供理论参考.     

肺肿瘤性磨玻璃结节平均CT值测量相关技术的影响因素研究

目的 分析CT扫描剂量、重建算法、层面选择及胸部不同位置对纯磨玻璃肺结节(pGGN)平均CT值测量偏差度的影响,评价影响肺肿瘤性磨玻璃结节(GGN)平均CT值测量的因素.方法 选用CT值为-650 HU的不同直径球形模拟肺pGGN,应用东芝(佳能)64排螺旋CT,设置4种剂量等级自动管电流调制技术扫描,3种三维权重自适应迭代重建剂量降低(AIDR-3D)算法重建,测量1 mm薄层图像肺结节平均CT值,计算偏差度.结果 低剂量模式扫描时,小于10 mm的球形pGGN平均CT值的偏差度较大.3种AIDR算法重建下,相同直径球形pGGN平均CT值偏差度差异较小;pGGN直径越小,球形pGGN平均CT值偏差度越大.在球形pGGN中心层面测量CT值,平均CT值的偏差度最小;偏离中心层面越远,偏差度越大.胸部3个位置的pGGN平均CT值偏差度均不同,肺实质内平均CT值偏差度最小.结论 除重建算法,CT扫描剂量、测量层面选择及结节所在胸部位置对于pGGN平均CT值测量的精准度均可造成影响.     

Inverted U-Shaped Associations between Glycemic Indices and Serum Uric Acid Levels in the General Chinese Population: Findings from the China Cardiometabolic Disease and Cancer Cohort (4C) Study

Objective The relationship between serum uric acid(SUA)levels and glycemic indices,including plasma glucose(FPG),2-hour postload glucose(2 h-PG),and glycated hemoglobin(HbA1 c),remains inconclusive.We aimed to explore the associations between glycemic indices and SUA levels in the general Chinese population.Methods The current study was a cross-sectional analysis using the first follow-up survey data from The China Cardiometabolic Disease and Cancer Cohort Study.A total of 105,922 community-dwelling adults aged≥40 years underwent the oral glucose tolerance test and uric acid assessment.The nonlinear relationships between glycemic indices and SUA levels were explored using generalized additive models.Results A total of 30,941 men and 62,361 women were eligible for the current analysis.Generalized additive models verified the inverted U-shaped association between glycemic indices and SUA levels,but with different inflection points in men and women.The thresholds for FPG,2 h-PG,and HbA1 c for men and women were 6.5/8.0 mmol/L,11.0/14.0 mmol/L,and 6.1/6.5,respectively(SUA levels increased with increasing glycemic indices before the inflection points and then eventually decreased with further increases in the glycemic indices).Conclusion An inverted U-shaped association was observed between major glycemic indices and uric acid levels in both sexes,while the inflection points were reached earlier in men than in women.     

中国第9次北极科学考察期间科考队员睡眠质量分析

目的 了解北极特殊环境对科考队员睡眠质量的影响.方法 应用匹兹堡睡眠质量指数(PSQI)量表对参加我国第9次北极科学考察的科考队员(131人)在出行前、北极考察期间、考察结束后的睡眠情况进行调查,并记录队员的入睡时间点、晨起时间点、入睡时间、睡眠时间.结果 共发放问卷131份,回收有效问卷88份.88名队员中男77名、女11名,年龄为22～50(48.1±17.9)岁,包括船员18人、科考队员70人.考察期间队员的中位晨起时间点比出行前早180 min,入睡时间长于出行前及考察结束3个月后[(45.23±25.84)min vs(18.45±14.34)min、(19.50±12.57)min,P均<0.01],夜晚睡眠时间短于出行前及考察结束3个月后[(6.83±2.24)h vs(8.36±1.45)h、(8.14±1.45)h,P均<0.01].根据PSQI量表评分,考察期间队员的睡眠质量下降、入睡时间延长、睡眠时间缩短、睡眠效率下降、催眠药物使用增加,与出行前相比差异均有统计学意义(P均<0.01).结论 极地极昼及低温环境显著影响北极科学考察时科考队员的睡眠节律,导致入睡时间延长、睡眠时间缩短、助眠药物使用增加,这可能影响到队员的工作状态.     

National Nutrition and Health Systematic Survey for Children 0-17 Years of Age in China

The main purpose of the National Nutrition and Health Systematic Survey for children 0-17 years of age in China (CNHSC) was to collect basic data on the nutrition, development, and health status for children in different regions across China using evidence-based, reliable, and cost-effective approaches. Children and their parents or guardians from seven regions (south, southwest, north, northwest, eastern, central, and northeast China) in China were recruited. A multi-stage stratified randomized sampling method was used. Two provinces were randomly sampled from each of the seven regions, from which one urban district and one rural country were also randomly sampled, resulting in a total of 28 survey counties/districts. Dietary surveys, health examinations, laboratory testing, and questionnaires were used to collect dietary intake, nutritional status, child development, and health status information. Nutrition, health, and lifestyle assessment of children and their parents was determined using the Knowledge Attitude Practice (KAP) survey. Greater than 100,000 children (38,000 children < 6 years of age and 66,000 children 6-17 years of age) completed the survey. The survey provided comprehensive data on child nutrition and health status for future studies and will serve as the basis for an integrated nutrition and health improvement strategies proposal for children in China.     

Identification of Boseongicola sediminum sp. nov., a Novel Decabromodiphenyl Ether (BDE-209)-tolerant Strain Isolated from Coastal Sediment in Xiamen,China

Polybrominated diphenyl ethers (PBDEs) are a group of aromatic organic bromine compounds, which have been used in a broad array of polymeric materials (plastics, foams, resins, and adhesives) as brominated flame retardants. They are used in commercial and household products, including textiles, electronic equipment, airplanes, and automobiles, especially in China and the USA, because they are inexpensive with excellent flame-retardant effects[1]. During the past decade, over 14,000 tonnes of PBDEs have entered the market, and have become ubiquitous environmental contaminants[2].     

乌司他丁与内质网分子伴侣免疫球蛋白结合蛋白的分子对接和结合作用

目的 通过测定乌司他丁与免疫球蛋白结合蛋白（Bip）在脑缺血再灌注模型大鼠血清、大脑皮层中的浓度,以及两者的体外结合实验、模拟分子对接模型,探讨两者之间的相互作用。方法将36只雄性SD大鼠随机分为两组。正常组(n=18)大鼠经静脉注射乌司他丁20万IU/kg作为对照。实验组(n=18)大鼠在建立大脑中动脉栓塞模型（MCAO）后随即静脉注射乌司他丁20万IU/kg。采用酶标仪法、ELISA法分别测定乌司他丁及Bip在大鼠脑缺血后05、3、24 h中血清、大脑皮层中的浓度。通过乌司他丁与Bip的结合实验计算两者结合率,并利用软件模拟乌司他丁与Bip的分子对接模型。结果在患侧大脑皮层中,实验组乌司他丁浓度在3 h升高,24 h下降,而Bip浓度则在3 h中下降,24 h升高。乌司他丁与Bip的结合率为44%～62%,分子对接结果显示乌司他丁与Bip中的残基可形成范德华力及共轭作用,两者可形成不稳定复合物。结论在缺血大脑皮质中乌司他丁可与Bip形成不稳定复合物,其可能通过影响Bip的表达而在脑缺血再灌注中发挥着一定作用。     

高龄女性累积胚胎后移植与非累积胚胎移植的妊娠结局分析

目的 比较40岁以上高龄女性累积胚胎后移植与非累积胚胎进行移植的妊娠结局。方法选择40岁以上且使用自身卵子进行冻融胚胎移植助孕229例不孕症妇女共338个周期资料,根据患者是否累积胚胎移植分为两组分析妊娠结局。 结果40~49岁女性随着年龄增高,基础卵泡刺激素水平升高（P<0.05）,获卵数减少（P<0.05）,临床妊娠率和持续妊娠率下降（P<0.05）。在40岁妇女中,累积胚胎移植组的临床妊娠率和持续妊娠率均高于非累积胚胎移植组（42.9% vs. 24.6%,P<0.05）,对于41岁、42岁、≥43岁,两组的临床妊娠率、持续妊娠率及早期流产率差异均无统计学意义（P＞0.05）。结论对于40岁不孕妇女,多次取卵累积胚胎后移植有助于提高临床妊娠率,对41岁以上女性累积胚胎对于助孕结局的益处有限。