碱性成纤维细胞生长因子对新生鼠缺氧缺血性脑损伤胶质纤维酸性蛋白表达的影响

目的探讨外源性碱性成纤维细胞生长因子（bFGF）对新生大鼠缺氧缺血性脑损伤（HIBD）后海马胶质纤维酸性蛋白（GFAP）表达的影响。方法通过结扎并剪断7日龄新生Wistar大鼠右侧颈总动脉，吸入8％氧气和92％氮气2h制备新生鼠HIBD模型，设假手术组、生理盐水对照组、bFGF治疗组。通过免疫组织化学方法和计算机图像分析技术检测3组大鼠不同时点（术后d4、7、10、17、24）海马CAI区GFAP表达强度变化。结果假手术组海马内GFAP阳性细胞数量和染色强度术后d7达高峰：对照组、治疗组GFAP表达较假手术组增多，治疗组增多更明显，术后d10达高峰，GFAP阳性细胞主要分布于海马CAI、CA3区．术后d4、10、17组比较差异有显著性（P均〈0．05）。结论1．新生鼠HIBD后脑缺血易损伤区GFAP表达增加，可能与脑损伤后神经细胞再生有关；2．外源性给予bFGF可增强新生鼠脑缺氧缺血后中枢神经系统GFAP表达，在神经细胞损伤的修复中发挥一定保护作用。     

静脉血营养静脉皮瓣的实验研究

目的：研究静脉血营养静脉皮瓣的成活机理。方法：20只成年兔被分成实验组和对照组，实验组制成阻隔式皮瓣。站果：阻隔式静脉皮辩的成活率与原位缝合静脉皮瓣差异无显著性（P＞0．05）。站论：受区与静脉皮瓣之间的侧支吻合对静脉皮瓣成活的影响不大。     

紫花茄皂苷Ⅰ-2对人肝癌细胞的细胞毒影响

目的 探讨紫花茄皂苷Ⅰ-2对人肝癌BEL-7402细胞的细胞毒作用.方法 用酸性磷酸酶(APA)法测定不同浓度紫花茄皂苷Ⅰ-2处理人肝癌BEL-7402细胞后的增殖抑制率和IC50.结果 紫花茄皂苷Ⅰ-2对BEL-7402细胞有明显的增殖抑制作用,在皂苷作用72 h后,实验组与对照组的A405比较差异有显著性(P＜0.05),半数抑制浓度IC50为6.2 μg/ml.结论 紫花茄皂苷Ⅰ-2对人肝癌细胞的细胞毒作用呈明显的浓度依赖性.     

两种侧卧法纠正枕横位的临床观察

目的探讨产程中产妇采用两种不同侧卧位纠正枕横位的临床效果。方法选择活跃期经内诊确诊为枕横位的初产妇192例,随机分成对侧卧位组(A组)和同侧卧位组(B组)各96例,分别采用对侧和同侧卧位法纠正胎方位,观察两组分娩方式及胎方位纠正为枕前位成功率、宫口开全所需时间、产后出血发生率、新生儿窒息发生率。结果①对侧卧位组阴道分娩87例(90·62%),胎儿转至枕前位72例(75·00%),同侧卧位组阴道分娩74例(77·08%),胎儿转至枕前位58例(60·42%),两组比较,有显著性差异(p<0·05)。②宫口开全所需时间比较,对侧卧位组比同侧卧位组短,有显著性差异(p<0·05)。③产后出血发生率、新生儿窒息发生率比较,无显著性差异(p>0·05)。结论产程中指导产妇取对侧卧位矫正枕横位,是提高阴道分娩率、加快产程进展的有效方法,值得推广。     

血清中异丙酚的HPLC测定

建立了检测血清中异丙酚的反相高效液相色谱-荧光法.血清样品经含内标百里酚的丙酮溶液直接沉淀后,采用KR100-5 C18柱,甲醇-0.1%三氟醋酸溶液(80:20)为流动相,荧光激发波长为276nm,发射波长为307nm.异丙酚在0.05～12.8μg/ml范围内与峰面积比线性关系良好.检测限为150pg,异丙酚和百里酚的提取回收率均大于90%,批内、批间精密度为1.11%～6.88%.     

中药川芎的指纹图谱分析

本研究对川芎原药材进行了提取处理,水提取物经萃取后获得化学组分。采用高效液相-质谱联用法(HPLC-MS)分析发现,在本实验条件下,用HPLC-MS能对指纹图谱的主要特征峰对应的化学成分进行分析,发现有相同的分子离子峰[M+H]+(m／z 225),易形成碎片离子m／z 207,并进一步阐明洋川芎内酯成分主要碎片离子峰的形成过程。     

不同灸法治疗原发性痛经的临床研究进展

目的:通过对近十年不同灸法治疗原发性痛经的临床研究文献进行整理分析,了解各种灸法治疗原发性痛经的临床疗效,以期择优选择适宜灸法治疗原发性痛经。方法:以"灸""原发性痛经""功能性痛经"为主题词,在中英文数据库上检索近十年(2010年1月—2020年2月)灸法治疗原发性痛经临床研究文献。结果:临床上灸法治疗原发性痛经的方法众多,主要以温和灸、温针灸、热敏灸为主,应用灸法治疗原发性痛经最多的证型是寒凝血瘀型(或寒湿凝滞型),但大部分临床研究未进行辨证给予艾灸治疗痛经以及同种证型痛经应用不同灸法的疗效差异未进行比较。结论:各种灸法治疗原发性痛经的临床疗效均较显著,但疗效差异不详,故今后要开展更多高质量的临床研究不同灸法治疗痛经之间疗效差异,明确不同证型痛经所适宜的灸法以提高临床疗效。     

Individual and joint effects of borderline ankle-brachial index and high plasma total homocysteine on all-cause death in hypertensive adults

BACKGROUNDThe cardiovascular hazards of total homocysteine (tHcy) are long known. In addition, despite the acknowledgment on the importance of low ankle-brachial index (ABI) (< 0.9), borderline ABI (0.91-0.99) was once commonly overlooked. This study aims to explore the independent and joint effect of tHcy level and borderline ABI on all-cause death in hypertensive population.METHODSThis study included 10,538 participants from China H-type Hypertension Registry Study. ABI was described into two groups: normal ABI (1.00-1.40) and borderline ABI. tHcy level was also divided into two groups: < 15.02 and ≥ 15.02 μmo/L. Four groups were analyzed, using COX proportional hazard regression model, separately and pairwise to observe the independent and joint effect on all-cause death.RESULTSA total of 126 (1.2%) deaths were observed in the 1.7 years follow-up time. Borderline ABI has a higher predicted risk of death than normal ABI (HR = 1.87, 95%CI: 1.17-3.00) after adjusting for potential covariates. Compare with tHcy level < 15.02 μmo/L (low tHcy), those with tHcy ≥ 15.02 μmo/L (high tHcy) had higher risk to event outcome (HR = 1.99, 95% CI: 1.30-3.05). According to the cumulative hazard curve, group with borderline ABI and high tHcy level has significantly higher altitude and larger increasing rate over follow-up period compare to other groups. Among those with borderline ABI, participants with high tHcy had higher death risk than those with low tHcy, nevertheless, no significant different between borderline and normal ABI among those with low tHcy levels.CONCLUSIONSBorderline ABI and tHcy level both have independent predictive value on all-cause death. The combined group of borderline ABI and high tHcy has highest risk factor of outcomes, which suggested the mutual additive value of borderline ABI and tHcy. More attention should be given to the importance of borderline ABI in hypertensive population, especially with elevated tHcy level.

Homocysteine (Hcy) is a sulfur-containing, non-proteinogenic amino acid synthesized through the transmethylation of amino acid methionine from one-carbon metabolism. Elevated plasma total homocysteine (tHcy) level is associated with endothelial dysfunction, increased blood coagulation, and metabolic disturbance, promoting cardiovascular diseases, stroke, and coronary artery disease.^[1,2] Notably, patients with high Hcy levels and concomitant hypertension were suggested to be at particularly higher risk.^[3] Moreover, increasing studies have explored a positive association between advanced Hcy level with all-cause mortality. According to a recent dose-response meta-analysis, for each 5-μmol/L increment of tHcy levels, the risk for all-cause mortality increased by 33.6%.^[4]The ankle-brachial index (ABI) is an effective, well-established measure that is commonly used in the diagnosis of peripheral artery disease (PAD),^[5] meanwhile was well studied as an important indicator of atherosclerosis and CVD events.^[6] Although ankle-brachial index (ABI) ≤ 0.90 has been recognized as the threshold value for abnormal/low ABI, which was proven to increase the risk of all-cause mortality,^[7] a study from the American Heart Association has suggested ABI between 0.91 and 1.00 should be considered as “borderline area” in terms of cardiovascular risks,^[8] considering of prior probability and sensitivity of ABI calculation. Emerging studies have aimed to explore the predictive value of borderline ABI,^[9-11] however, controversy remains because of limited and inconsistent data. The current study aimed to explore the individual and joint effect of borderline ABI and tHcy on all-cause mortality among hypertensive adults. Although ABI level ≤ 0.90 has been and is going to remain significant in clinical practice, we believe broader concern should be placed on borderline ABI, especially for its value in risk differentiation and identification. To the best of our knowledge, there are no similar previous studies.     

牙乳头细胞基本特征及其研究进展

牙乳头细胞来源于外胚间充质,具有多向分化潜能,是体内唯一分化为成牙本质细胞的前体细胞。该细胞在牙发育和牙体牙髓损伤修复过程中起重要作用。牙乳头细胞相关的组织工程化研究是近年来的热点。下面就牙乳头细胞的形态及其功能特征、三维细胞培养、组织工程化牙研究进展等方面的研究作一综述。     

从玄府气液理论探讨偏头痛的病机及治疗

偏头痛是一种常见的神经-血管功能障碍性疾病。玄府是遍布机体各处的一种至微至小的组织结构,其主要功能包括流通气液、渗灌气血和运转神机。脑玄府开阖不利是头痛各种证候形成的共同基础,气郁玄府、浊停玄府、血瘀玄府或玄府气血不足,是头痛重要的发病机制。治以开通玄府法,以畅达人体阻滞之气、血、津、液,用药多选用祛风、理气、活血、化痰、通络之品。