重型肝炎TGF-α、TNF-α、IL-1β改变的初步研究

研究重型肝炎病程中TGF-α、TNF-α、IL-1β的改变。23例进入研究,用放免法集中检测血清标本。急性期的三种细胞因子均显著高于正常对照(P<0.001)。恢复的TGF-α高于急性期(P=0.092)。TNF-α、IL-1β升高不明显(P=0.944,P=0.73s)。存活组14例,未存活组9例。急性期、恢复期或病情恶化时的三种细胞因子均显著高于正常对照(P<0.05)。存活组恢复期的TGF-α显著高于急性期(P=0.042),TNF-α、IL-1β比急性期升高,但差异不显著(P=0.948,P=0.992)。未存活组病情恶化时的TGF-α、IL-1β也明显高于急性期(P=0.050,P=0.062),TNF-α略升高(P=0.567)。两种炎症细胞因子在重型肝炎的发生、发展中起重要作用。TGF-α的血清浓度似乎影响病情转归。     

Behçet's syndrome coexisting with clinically occult ankylosing spondylitis

The case of a 36-year old man with Beh?et's disease (BS) for 16 years had low back pain and stiffness in the cervical and lumbar spine. He was diagnosed as having ankylosing spondylitis (AS). In this report we wish to emphasize the clinically occult co-existence of AS in BS and revelation of AS after a long time of BS diagnose.     

LAK细胞和单克隆抗体MGb2的协同抗胃癌作用

在LAK细胞对胃癌细胞KATOⅢ的杀伤过程中,加入单克隆抗体MGb2能产生明显协同作用。我们认为此作用的原理是抗体依赖细胞介导的细胞毒性作用(ADCC)。此作用随着制备LAK细胞时白细胞介素2(IL-2)浓度的增加而增高.与LAK活性呈平行关系:制备LAK细胞时,IL-2的诱导时间既影响LAK活性,也影响相应的ADCC作用。     

并殖吸虫尾蚴直接感染动物的实验研究

本实验为尾蚴直接感染终末宿主而患肺吸虫病的可行性研究。从动物选择、尾蚴感染数量与活动力和感染方法三个方面,做了适当的改进,使尾蚴直接感染的6只猫和5只犬中,有3只猫、1只犬感染成功。其感染发病率为36.3％,并取得了感染发病的完整资料。     

Brucella endocarditis: the importance of surgical timing after medical treatment (five cases)

INTRODUCTION: Brucella endocarditis is a disease that is hard to treat medically and has a high mortality. Immediate surgery after medical treatment is very important because delaying surgery may lead to that are difficult to repair. METHODS: Five patients who were admitted to our institution with a diagnosis of Brucella endocarditis were medically treated with doxycycline (200 mg/d), rifampin (600 mg/d), and ceftriaxone (2 g/d). Preoperative mean medical treatment time was 5.2 weeks (range, 4-6 weeks). The patients were taken for operation when their general status improved. We report in this study the results of these patients. RESULTS: Three patients had aortic valve replacement whereas 2 had both aortic and mitral valve replacements. No mortality or morbidity was encountered in the patients. Mean postoperative hospitalization time was 15 days (range, 12-19 days). The patients were discharged with doxycycline (200 mg/d) and rifampin (600 mg/d) but without antipyretic medication. Postoperative antibiotherapy was continued up to a mean of 3.6 months (range, 2-6 months). Mean postoperative follow-up time was 15.8 months. None of the patients needed hospitalization in their follow-up time. CONCLUSION: Adequate preoperative antibiotherapy, immediate surgery, and continuation of postoperative antibiotherapy according to clinical progress seem to be a convenient treatment strategy for Brucella endocarditis.     

A Novel In Vivo Rabbit Model of Abdominal Aortic Aneurysm Induced by Periarterial Incubation of Papain

PurposeThe objective of this study was to determine the possibility of creating a novel animal model of abdominal aortic aneurysm (AAA) in rabbits by the periarterial application of papain.Materials and MethodsTwelve New Zealand white rabbits were randomized into two groups: (1) the papain group, which received 2 mg of papain (n=8) and (2) the control group, which received physiologic saline solution (n=4). A 1-cm aortic segment proximal to the bifurcation was isolated, and its adventitia was incubated with papain for 20 minutes. The rabbits underwent intravenous digital subtraction angiography (IVDSA) 5 and 21 days after the operation. The animals were then humanely killed for histomorphometric and immunohistochemical studies.ResultsAll animals in the papain group developed AAA, with an average aneurysm diameter of 4.0±0.6 and 4.1±0.4 mm on days 5 and 21, respectively. No aneurysms were seen in the control group. On day 5, the papain-incubated aortas exhibited thinned and disorganized aortic walls, with decreased smooth muscle cells (SMCs) and fragmented and almost nonexistent elastic lamella. Media thickening, intimal hyperplasia, and smooth muscle cell regeneration were obvious on day 21. Immunostaining of matrix metalloproteinase (MMP)-9 and RAM11 showed strong expression in the papain group. On the contrary, the control group did not present histologic alterations and showed almost no expression of MMP-9 and RAM11.ConclusionsA novel in vivo rabbit model of AAA can be induced through periarterial application of papain for 20 minutes. This model is similar to an elastase-induced aneurysm model and could be useful to clarify AAA pathogenesis and endovascular treatment intervention.     

Imaging diagnosis, transurethral endoscopic observation, and management of 43 cases of persistent and refractory hematospermia

The goal of this study was to explore minimally invasive transurethral imaging and surgery for the treatment of severe, persistent hematospermia in cases that were refractory to conservative treatments. The study included 43 patients (aged 22-77 years; average, 44.6 years) with long-lasting, severe hematospermia, accompanied by discomfort or pain in the lumbosacral or perineal region, dysuria, frequent micturition, decreased semen volume, and/or azoospermia. Patient symptoms had persisted for 1 to 10 years (average, 5.3 years). Computed tomography or magnetic resonance imaging of each patient was evaluated, and transurethral surgery was performed. The causes of hematospermia were identified in all 43 patients, and their ejaculatory duct obstruction or seminal vesiculitis was successfully treated. No serious intraoperative or postoperative complications occurred. Pathologic analyses revealed that all of the resected or biopsied seminal vesicle tissues had chronic nonspecific inflammation in the seminal vesicle wall, and no tumors were identified. Preoperative symptomology of hematospermia disappeared in all patients followed up for 2 to 30 months (average, 16 months). A single patient experienced recurrence at 11 months and had a second minimally invasive surgery that was curative. A total of 95.3% (41 of 43) of the patients experienced normal orgasmic intensity after surgery. Magnetic resonance imaging is a valuable and accurate diagnostic method for the identification of causative factors underlying hematospermia. Transurethral dilation of ejaculatory ducts, incision of the verumontanum or the distal end of the ejaculatory ducts, and incision or resection of the relevant cysts represent simple, safe, and reliable approaches for the management of refractory cases of hematospermia that do not respond to conservative treatments.