Inactivation of the Rho-ROCK signaling pathway to promote neurologic recovery after spinal cord injuries in rats

Background After injury,axonal regeneration of the adult central nervous system （CNS） is inhibited by myelin-derived growth-suppressing proteins.These axonal growth inhibitory proteins are mediated via activation of Rho,a small GTP-binding protein.The activated form of Rho,which is bound to GTP,is the direct activator of Rho kinase （ROCK） through serial downstream effector proteins to inhibit axonal regeneration.The objective of this study was to observe the therapeutic effect of inactivation of the Rho-ROCK signaling pathway to promote neurologic recovery after spinal cord injuries in rats.Methods One hundred and twenty adult female Sprague-Dawley rats were randomly divided into three groups.Laminectomies alone were conducted in 40 rats in the sham group.Laminectomies and spinal cord transections were performed in 40 rats in the control group （treated with normal saline administered intraperitoneally）.Laminectomies and spinal cord transections were performed in 40 rats in the fasudil-treated group （treated with fasudil administered intraperitoneally）.Neurologic recovery was evaluated before surgery and 3 days,and 1,2,3,and 4 weeks after surgery using the Basso-Beattie-Bresnahan （BBB） scale of hind limb movement.At the same time,the expression of RhoA mRNA was determined with RT-PCR.Histopathologic examinations and immunofiuorescence staining of NF were performed 1 month after surgery.Results Compared with the control group,the BBB scores of the fasudil-treated group were significantly increased and the expression of RhoA mRNA was significantly decreased.In the fasudil-treated group,a large number of NF-positive regenerating fibers was observed; some fibers crossed the slit of the lesion.Conclusion Inactivation of the Rho-ROCK signaling pathway promotes CNS axonal regeneration and neurologic recovery after spinal cord injuries in rats.     

Huge trichilemmal carcinoma of the scalp

To the editor： Trichilemmal carcinoma （TLC） is an uncommon cutaneous adnexal malignant tumor that develops from the external root sheath of the hair follicle. It usually appears as an exophytic nodule measuring less than 3 cm with ulcer in the middle. Here, we report a case of a patient who developed a huge TLC on his scalp in a 10-year long course, which had been palliatively excised for three times. Clinical characteristics, differential diagnosis and surgical treatment are discussed.     

帕瑞昔布钠用于腹腔镜胆囊切除术多模式镇痛的疗效分析

目的 探讨帕瑞昔布钠用于多模式镇痛对腹腔镜胆囊切除术后疼痛的影响.方法 采用前瞻、双盲、随机、对照研究的方法,选择于2011年3至6月在温州医学院附属第一医院腔镜外科择期行腹腔镜胆囊切除术患者80例,随机均分为两组,试验组术前30 min静脉注射帕瑞昔布钠40 mg,术后12、24、36、48 h分别静脉注射帕瑞昔布钠40 mg,对照组于相同时点静脉注射生理盐水各2 ml.两组患者均于手术结束时予罗哌卡因局部浸润麻醉手术切口.术后1、2、4、8、12、24、36、48 h采用视觉模拟评分法(VAS)评估两组患者疼痛程度,并记录术后24h内哌替啶用量.结果 试验组术后各时点VAS评分(1 h:1.0±0.6、8 h:2.5±1.4、24 h:1.6±0.8、48 h:1.0 ±0.5)明显低于对照组(1 h:1.8±0.6、8 h:5.0 ±1.8、24 h:2.5 ±1.4、48 h:1.5±0.6)(P＜0.01),且术后24h内试验组哌替啶的用量明显少于对照组(P＜0.01).结论 静脉注射帕瑞昔布钠40 mg用于多模式镇痛可有效减轻腹腔镜胆囊切除术后疼痛.     

神灯联合多磺酸黏多糖乳膏治疗老年患者外周血管硬化的效果观察

目的观察神灯联合多磺酸黏多糖乳膏（喜疔妥）在治疗老年患者外周血管硬化中的效果。方法选取2012年6月～2013年1月在本院住院治疗的伴有外周血管硬化的患者34例,随机分成两组．实验组18例,用神灯照射联合喜疗妥外敷进行治疗：对照组16例,单纯采用喜疗妥外敷治疗,观察并记录两组患者的治疗效果。结果实验组总有效率为94．4％。对照组总有效率为62．5％．实验组的治疗效果明显优于对照组,差异具有统计学意义（P〈0．01）;实验组中血管壁厚度及血管腔狭窄的改善程度明显优于对照组（P〈0．05或0．01）。结论在老年患者外周血管硬化的治疗中,神灯照射联合喜疗妥疗效果明显优于单纯采用喜疗妥。     

A new sesquiterpene lactone from the fruits of Hlicium henryi

AIM： To study the chemical constituents of the fruits oflllicium henryi. METHOD： Chromatographic separations on silica gel, Sephadex LH-20 gel and MCI gel were used to isolate the compounds. The structures were elucidated based on extensive spectroscopic data analyses. RESULTS： Seven compounds were obtained and their structures were identified as 10-benzoyl-cycloparvifloralone （1）, cyclopar- vifloralone （2）, 2a-hydroxycycloparvifomlone （3）, henl-ylactone B （4）, merrillianone （5）, henrylactone C （6） and 7, 14-ortholactone- 3 -hydroxyfloridanolide （7）. CONCLUSION： Compound 1 is a new sesquiterpene lactone. The tested compounds showed weak anti-HBV activities on HBV surface antigen （HBsAg） secretion and HBV e antigen （HBeAg） secretion using Hep G2.2.15 cell line.     

超声诊断腮腺类肿瘤型舍格伦综合征1例

病例 男,35岁,因右侧面部肿块于2012年3月13日到我院口腔科就诊.患者20天前发现右侧面部耳前下方处一肿块,无疼痛,在其他医院诊断为腮腺炎症,用抗菌药物治疗1周肿块无消退后到我院就诊.患者无口下唾液减少的症状,无眼睛干燥症状.查体:右侧面部耳前下方处触及一肿块,直径约30 mm,肿块质地较硬,无活动度,无明显压痛.     

屋尘螨提取液致敏BALB/c与C57BL/6小鼠肺部变应性炎症模型的比较

目的研究以屋尘螨提取液复制BALB/c和C57BL/6小鼠肺部变应性炎症模型的方法.方法BALB/c和C57BL/6小鼠各分为对照组、加强组和常规组.加强组在第1天采用皮下注射,以后隔日采取腹腔注射进行致敏,再用提取液多次滴鼻激发(第15～17天).最后1次滴鼻24 h后对小鼠作肺泡灌洗.常规组则于第1、8天腹腔注射2次,第15～17天滴鼻3次.对照组用PBS作注射和滴药.进行灌洗液细胞计数和分类,肺组织病理计分,灌液洗用ELISA测定IL-2、IL-4、IL-5和IFN-γ含量.结果①C57BL/6加强组肺部病理计分明显升高,呈明显变应性炎症病理改变;灌洗液中细胞计数和嗜酸性粒细胞计数明显升高(P＜0.05);灌洗液IL-4、IL-5明显升高(P＜0.05),IFN-γ、IL-2无明显变化;②BALB/c加强组、腹腔注射致敏组和C57BL/6腹腔注射致敏组可见炎性细胞浸润,但嗜酸性细胞较少,BALF中IL-4、IL-5升高较加强组为弱,灌洗液中细胞计数增高,但嗜酸性细胞计数较对照组无明显差异,③单剂量组上述指标与对照组比较无显著差异.结论与BALB/c相比,在屋尘螨致敏小鼠哮喘模型的复制中,C57BL/6更易取得成功,且使用皮下注射加强致敏C57BL/6小鼠所复制屋尘螨致敏哮喘模型更为典型.     

重组牛碱性成纤维细胞生长因子联合藻酸盐敷料治疗老年人慢性溃疡创面

目的:观察外用重组牛碱性成纤维细胞生长因子(rb-bFGF)联合藻酸盐敷料治疗老年人慢性溃疡创面的临床效果.方法:将90例有慢性溃疡创面的老年患者按收治的先后顺序随机分为联合组(采用rb-bFGF联合藻酸盐敷料治疗)、bFGF组(采用rb-bFGF治疗)和常规组,每组30例.比较3组治疗第1、7、14、21天创面渗液量...     

沉默视黄酸核受体α损伤大鼠原代海马神经元的钙兴奋性

目的:了解视黄酸核受体α (RARα)对大鼠神经元功能的必要性.方法:采用组织消化原代贴壁法分离培养大鼠原代海马神经元,利用腺病毒载体特异沉默RARα;利用Real-Time PCR分析沉默RARα对神经元视黄酸(RA)信号各受体以及神经细胞标志物的影响;利用活细胞钙影像分析沉默RARα对神经元钙兴奋性的影响.结果:免...     

实时心肌超声造影微泡再充盈参数评价心肌梗死患者心肌灌注

目的 应用实时心肌超声造影分析收缩末期与舒张末期再充盈参数,探讨其评价心肌梗死患者心肌微循环灌注的可行性.方法 对21例陈旧性心肌梗死及急性心肌梗死亚急性期的患者及6例正常对照组进行实时心肌超声造影.用时间强度曲线分析左室各节段收缩末期及舒张末期的微气泡再充盈过程.根据灌注的函数公式y(t)=A[1 - e-kt]+B,脱机心电图触发方式选择收缩末期(T波结束)帧和舒张末期(R波顶点)帧分别执行灌注曲线拟合,得到微气泡信号强度增加的高度(A值)、信号强度增加的速率(k值)并计算A×k值,比较收缩末期与舒张末期相应节段心肌血容量、心肌血流速度、心肌血流量的差异.结果 正常对照组中收缩末期的A值、k值及A×k值均小于舒张末期各值,差异有统计学意义[分别为(6.21±2.69)dB对(7.93±3.66)dB,P＜0.05;0.36±0.15对0.42±0.19,P＜0.01;2.88±1.29对3.39±1.61,P＜0.05].与收缩末期相比,舒张末期A值,k值及A×k值具有更大的变异性(变异系数分别为A值46.2％对43.3％,k值45.2％对41.4％,A×k值47.5％对44.8％,P值均＜0.05).病例组中梗死相关冠状动脉供血节段心肌的收缩末期再充盈参数明显小于非梗死冠状动脉供血心肌各参数值,差异有统计学意义[A值、k值及A×k值分别为(3.99±1.02) dB对(4.77±1.24)dB,P＜0.05;0.31±0.13对0.35±0.16,P＜0.05;1.25±0.74对2.39±1.11,P＜0.01].结论 收缩末期与舒张末期再充盈参数可以评价心肌微循环灌注,收缩末期各参数值具有更小的变异性,其中收缩末期k值是一个较好的评价指标.然而,显著的变异性提示这些指标适用于自身前后对照研究.