基于Web和SQL／ASP技术构建胆石病数据挖掘系统

目的探讨基于Web和SQL/ASP技术、采用B/S模式构建胆石病数据查询和挖掘系统的方法,验证胆石病中医病因病机诊断标准和结果的准确性.方法以胆石病患者的临床病案资料为基础,构建胆石病数据查询和挖掘系统,同时运用SQL Server 2005建立胆石病中医病因病机Logistic Regression模型,对胆石病临床症状进行分析,预测中医病因病机的分布.结果建立了基于Web & SQL/ASP技术、采用B/S模式结构的胆石病数据查询和挖掘系统,设计了胆石病中医病因病机Logistic Regression模型.结论基于Web和SQL/ASP技术设计的医用信息系统,不仅能高效地管理和利用医院各类信息资料,便于归档和查询,同时通过对不断累积的数据进行挖掘,能为理论研究和临床实践提供更精准的信息支持.     

Autologous bone marrow transplantation in children with acute lymphoblastic leukemia

We report 25 children with acute lymphoblastic leukemia (ALL) treated with purged autologous bone marrow transplantation (ABMT) at a single center. Two children with high-risk ALL were transplanted in first remission and 23 with relapsing ALL were transplanted in second (n = 21) or third (n = 2) remission. There was no procedure-related mortality. The median time to engraftment (i.e. to reach a polymorphonuclear cell count of 0.5 x 10(9)/l) was 25 days (range 16-45 days). Seven children relapsed, four within five months after ABMT: 18 of 25 children (72%) are in continuous complete remission after a median follow-up period of 50 months (range 5-71 months). The predicted long-term disease-free survival is 65% in the whole group and 61% in those transplanted after relapse. Relapse-free children returned to normal activities within three months after ABMT. The major side effects were development of cataract and gonadal insufficiency. We consider the results promising, but our data do not allow comparison with results reported from treatment with chemotherapy alone, since some of our patients were referred from other centers and represent a selected patient group. Long-term follow-up of well-defined patient populations is necessary to evaluate the effect of ABMT.     

羊膜和角膜干细胞移植治疗眼表疾病临床观察

目的探讨羊膜移植、自体角膜干细胞移植及自体体外培养角膜干细胞移植重建眼表治疗眼表疾病的疗效。方法眼表疾病43例46眼（瘢痕性结膜囊狭窄、睑球粘连6例6眼，角结膜化学、热烧伤10例13眼，原发和复发性翼状胬肉切除术后结膜缺损27例27眼）进行羊膜移植、自体角膜干细胞移植及自体体外培养角膜干细胞移植，观察植片及角结膜上皮愈合、组织浸润和新生血管情况。结果羊膜移植术重建结膜囊效果满意，睑球粘连松解。羊膜、自体体外培养角膜干细胞移植术治疗角结膜烧伤均可使眼表上皮化，基质炎性浸润减轻，新生血管明显减少；自体体外培养角膜干细胞移植对角膜缘干细胞损伤严重的患者效果显著。自体和自体体外培养角膜干细胞移植治疗翼状胬肉均有良好效果，术后不适症状轻时间短，角膜创面愈合快，随访（1～2）y两种术式各复发1眼。自体体外培养角膜干细胞移植对复发性翼状胬肉切除术后较大的结膜缺损更为适合。结论羊膜移植、自体角膜干细胞移植和自体体外培养角膜干细胞移植对重建眼表都有重要的功效，手术方式的选择必须根据角膜干细胞损伤的程度、范围进行选择。     

胃肠道无线内窥镜中可吞服摄像药丸的研究

介绍无线摄像药丸直径11mm,长30 mm,包括CMOS图像传感器、光学系统、电池、发射电路及天线等.利用MEMS工艺将CMOS图像传感器和驱动电路微型化,也可将发射电路集成化.图像信号有模拟和数字两种传输方式.数字方式使用串行数据接口传输图像.最后介绍药丸外壳的加工工艺.     

儿童体外循环内源性类洋地黄物质变化

目的 探讨内源性类洋地黄物质与儿童心功能之间的关系及其在体外循环期间变化的临床意义。方法 80例先天性心脏病患儿根据心功能分级分为4组，按先天性心脏病分类分为2组，于术前，主动脉阻断后，转流30min，心脏复苏后和术后24h测定血浆EDLS浓度。结果 (1)儿童血浆EDLS浓度在心功能代偿期与正常对照组无显著性差异，而心功能不全各级分别显著高于正常组，并随心功能不全程度加重而增加；(2)体外循环期间以循环阻断后EDLS浓度最低，随着心脏复苏EDLS浓度逐渐升高，至术后24h EDLS浓度恢复至术前水平；(3)青紫型先天性心脏病与非青紫型先天性心脏病比EDLS血清浓度有显著性差异，而转流期间变化不大。结论 (1)EDLS血浆浓度与心功能不全的严重程度呈密切相关，成为维持心脏功能代偿的因素之一；(2)EDLS随容量负荷增加而增加；(3)儿童心脏病术后适当应用洋地黄制剂是安全的。     

Cytofluorescence localization of adriamycin in the nervous system

Summary By a fluorescence-microscopic technique, the distribution of the antineoplastic glycoside adriamycin (doxorubicin) was studied in the peripheral nervous system (PNS) of normal adult mice after i.v. injection. Doses comparable to those used in patients for treatment of malignant diseases were used.The orange-red fluorescence of the drug was observed in dorsal root ganglia, in the trigeminal ganglia, and in the superior cervical sympathetic ganglia where it was preferentially accumulated in the nuclei of satellite cells. This nuclear labeling was a very quick process which occurred in the superior cervical ganglion within 15 s after the injection. Adriamycin-fluorescent nuclei were also observed in the suprarenal medulla.Fluorescent nuclei were present within the pre- and postganglionic sympathetic nerve trunks close to the superior cervical ganglion but not in the endoneurium of the trigeminal and the sciatic nerves or in the spinal nerve roots. In such structures labeled cells appeared in the connective tissue sheaths covering the nerves and the roots. No adriamycin-induced fluorescence was detected in the myenteric plexus of the intestine.Our study thus shows that i. v. injected adriamycin is distributed preferentially within areas of the PNS where the blood vessels are known to be highly permeable.Supported by grants from the Swedish Medical Research Council Project 12X-03020     

Autoradiographic study of distribution patterns of metals which occur as corrosion products from dental restorations.

Radioactive chromium, cobalt, gold and mercury, all of which may occur as corrosion products from dental restorations, were injected in single doses via the tail vein of mice and an in vivo analysis of their distribution was performed using whole-body autoradiography. Gold seemed to be inert and went mainly to the liver and bones. The other elements studied were distributed widely in the body and remained for a long period of time in specific target organs and tissues.     

Normal spontaneous cortisol secretion in children after autologous bone marrow transplantation

AIM: To describe spontaneous cortisol secretion in children after autologous bone marrow transplantation (BMT) for acute leukaemia and lymphoma. METHODS: Spontaneous cortisol secretion was analysed in 39 children before and after BMT. Thirteen patients were conditioned with chemotherapy only (group 1), and 26 patients also with total body irradiation (TBI). In the TBI group, 14 patients had received no additional irradiation (group 2), whereas 12 patients had received cranial irradiation (CRT) previously (group 3). RESULTS: Before BMT, in comparison with group 1, mean morning cortisol was significantly lower in group 2 (252 vs 415 mmol/l, p = 0.004), but not in group 3 (vs 312 mmol/l, p = 0.12). There was no change in group 1 six months after BMT (to 379 nmol/l), whereas morning cortisol increased significantly in group 2 and group 3 (to 386 and 343 nmol/l, respectively; p < 0.05). The change in mean morning cortisol correlated negatively with pretransplant morning cortisol (r = -0.63, p < 0.001). Neither TBI nor CRT were associated with changes in morning cortisol. CONCLUSION: Spontaneous cortisol secretion is maintained after BMT irrespective of whether cranial or total body irradiation has been given or not.     

中国20个少数民族青少年体质发育状况的分析

目的分析我国20个少数民族青少年体质发育状况。方法利用与体质发育密切相关的生长发育指标(身高、体重、胸围、肺活量和身体质量指数)作20个民族间的聚类分析。结果20个民族聚合成主要的三大类,各类群间的体质发育存在明显差异。结论20个民族青少年的体质发育水平整体较低。